68 results about "Pseudomonas cerasi" patented technology

The invention relates to a bacterium S2 for efficiently generating biosurfactant and a fermentation culture medium thereof, belonging to the field of biotechnology. The bacterium S2 is gram-negative bacterium which is rod-shaped, is (6-8)*(8-12) micrometers in size and has no spores and capsules. The bacterial strain belongs to the pseudomonas aeruginosa through identifying by morphological and physio-biochemical characteristics and molecular biology, and the collection code is CGMCC No.3034. The best fermentation culture medium is a novel culture medium which takes 50g / L of rapeseed oil as the only carbon source. The rhamnolipid is the only fermentation product of the bacterial strain and has high yield. The CMC (Critical Micelle Concentration) value of the fermentation liquid is 0.25 g / L; compared with the common chemical surfactant, the bacterium has more obvious emulsification and durability on hydrophobic substances, such as oils and the like.

The invention discloses pseudomonas aeruginosa and an application thereof in degrading zearalenone. The preservation number of the pseudomonas aeruginosa is CGMCC No.8727. As a biologic material for degrading zearalenone, the pseudomonas aeruginosa is good in application prospect in no matter developing a novel biologic degrading bacterium agent or a biologic degrading bacterium-free preparation.

The invention relates to a phage for degrading a Pseudomonas aeruginosa biofilm. The phage is characterized in that: the phage is named C12, the collection number is CGMCC No. 4249, the collection unit is China General Microbiological Culture Collection Center located at No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Beijing City, and the collection date is October 26, 2010. The invention provides a method for amplifying, separating and preserving the phage for degrading Pseudomonas aeruginosa. The capacity of degrading the Pseudomonas aeruginosa biofilm by the phage is also determined, finally the pure phage C12 with high titer and high biofilm degradation capacity is obtained, and the degradation amount of the pure phage C12 reaches 85.3 percent; the phage can degrade biofilms and crack viable bacteria in the biofilms; the clinical application of the phage can be realized; treatment problems of medicine resistance and biofilms of the Pseudomonas aeruginosa are solved; and the method is a feasible biological method.

The invention relates to pseudomonas aeruginosa and an application thereof. The pseudomonas aeruginosa is named as Pseudomonas aeruginosa YM4 according to bacterial strain classification, and has a preservation number of CCTCC NO:M 2017494. The bacterial strain can produce rhamnolipid in a high yield from hydrophilic carbon sources, hydrophilic carbon sources and composite carbon sources, and therhamnolipid has a substrate conversion rate reach 65-80%. In a rhamnolipid product of the YM4 bacterial strain, di-rhamnolipid and single rhamnolipid are main products, and the amount of di-rhamnolipid and single rhamnolipid takes 80-90% of the total amount of glycolipid. According to the carbon source metabolic characteristic of the bacterial strain, a foam-free seed medium formula suitable for industrial application is developed, and the contamination rate of a seed tank is greatly reduced. The bacterial strain is high in rhamnolipid yield, conversion rate and ratio of a diglycolipid component, and has high industrial application value.

The invention relates to Pseudomonas aeruginosa FJAT-346 which is obtained by separating and screening an endosymbiotic bacterium in banana plants cultivated in fields with serious banana blight and has biologic al control action. The stain is Pseudomonas aeruginosa FJAT-346 and is used for preparing a fermentation liquid capable of controlling banana blight. The invention has the advantages that the endosymbiotic bacterium FJAT-346 is obtained by separating from the endosymbiotic bacterium in the banana plants, the fermentation liquid being capable of controlling banana blight and being prepared by using the stain has excellent inhibition action on banana blight bacteria, and the preparation process of the fermentation liquid is simple; and what's more important, the fermentation liquid is completely harmless and has good production application prospect.

The invention relates to an application of high-content dirhamnolipid of pseudomonas aeruginosa in the bio-remediation. The high-content dirhamnolipid produced by the fermentation of pseudomonas aeruginosa is mainly applied in the bio-remediation field of polluted soil or water body. The invention has the following advantages: the content of dirhamnolipid in the dirhamnolipid mixture is as high as 90%; by the use of the high-content dirhamnolipid, water surface tension can be reduced from 71.4 mN / m to 30.5 mN / m; the critical micelle concentration is 48mg / L; the high-content dirhamnolipid has high surface activity; under extreme conditions of high temperature, high salt and high pH and the like, the high-content dirhamnolipid can still maintain high surface activity and emulsifying capacity, can remarkably promote biodegradation of polycyclic aromatic hydrocarbons and has a latent application value in the bio-remediation.

The invention discloses a biocontrol bacterium for mulberry diseases and application thereof. The invention provides a pseudomonas aeruginosa strain for preventing and treating fusarium wilt of mulberry trees. The strain is preserved in the Guangdong Microbial Culture Collection Center on March 19th, 2019, and the preservation number is GDMCC No:60613. The pseudomonas aeruginosa strain with a goodantagonism / prevention and control effect on various pathogens of the fusarium wilt of the mulberry trees is obtained for the first time. It is safe and effective to conduct biological control on bacterial wilt disease of the mulberry trees by using the strain or fermentation broth of the strain, various problems such as soil environment deterioration and pathogenic drug resistance enhancement caused by chemical pesticides can be avoided, a foundation is laid for biological prevention and control of mulberry diseases, application prospects are good, and the biocontrol bacterium is worthy of wide popularization.

The invention relates to the bioengineering field, in particular to application of a phage lyase PlyHSE3 of food-borne pathogen bacillus cereus. The lyase involved in the invention has a nucleotide sequence shown as SEQ NO 1 and an amino acid sequence shown as SEQ NO 2. The phage lyase has high temperature tolerance and pH tolerance, can be used for prevention and control of bacillus cereus florabacteria, and the lyase can prevent and control pathogenic bacillus cereus under the condition of 4DEG C to 45DEG C. In addition, the lyase can also split pseudomonas aeruginosa. The invention provides a new enzyme preparation source for prevention and control of food-borne pathogen bacillus cereus, other bacillus cereus flora bacteria and pseudomonas aeruginosa at present.

The invention discloses a method for degrading petroleum by pseudomonas aeruginosa having a high rhamnolipid yield. The method comprises (1) acquisition of pseudomonas aeruginosa having a high rhamnolipid yield, (2) enlarge cultivation: inoculating a liquid medium with the pseudomonas aeruginosa obtained by the step 1, carrying out culture under shake culture conditions of 30+ / -1 DEG C and 150-180 rpm for 50-60h, carrying out centrifugation and filtration, collecting mycelia and drying the mycelia to obtain bacterial powder, and (3) degradation of petroleum: putting the bacterial powder obtained by the step 2 to the sea surface polluted by petroleum according to a ratio of the bacterial powder to the petroleum of 20-30g: 100g and carrying out natural degradation for 15-30 days. The method has the advantages of fast degradation rate, no residual hazard and low cost.

The invention discloses novel application of pseudomonas aeruginosa fluorescene ironophore, which is characterized in that the fluorescene ironophore can inhibit the growth of vibro splendidus in 2216E culture medium or sea water, and is derived from pseudomonas aeruginosa; the pure fluorescene ironophore is obtained by carrying out affinity chromatography on a copper NTA agarose column; 3 percent volume of purified fluorescene ironophore is added into a 2216E culture medium containing vibro splendidus to culture at 30 DEG C for 24 hours, and the inhibition rate on vibro splendidus can be 50 percent; 1 percent volume of the purified fluorescene ironophore is added into sea water containing vibro splendidus to incubate at 30 DEG C for 24 hours, and the growth of the vibro splendidus in the sea water can be completely inhibited. The pseudomonas aeruginosa fluorescene ironophore is expected to play an important role in inhibiting pathogen vibro splendidus.

The present invention relates to a pseudomonas aeruginosa phage and an application thereof, and particularly discloses the phage capable of entering blood and the application thereof. The phage singlebody is the pseudomonas aeruginosa phage with a Latin name P. Aeruginosaphage, and named as ASP11, and has a broad-spectrum bactericidal ability against pseudomonas aeruginosa. The phage ASP11 is preserved in China General Microbiological Culture Collection Center, a preservation date is September 26, 2018 and a preservation number is CGMCC NO.16395. Both in vivo and in vitro tests show that thephage has a strong cleavage effect on the pseudomonas aeruginosa and provides an effective method for prevention and treatment of pseudomonas aeruginosa infected diseases.

The invention relates to the field of microbial remediation environments, particularly discloses a pseudomonas aeruginosa and a fungicide with the pseudomonas aeruginosa, wherein the preservation number of the pseudomonas aeruginosa is CGMCC No.10849. The pseudomonas aeruginosa can survive and grow in the environments of a sole carbon source containing petroleum and / or petroleum products when inorganic salt culture media are provided, is high in life force, rapid to grow and large in biomass and has high tolerance and degrading capability for the petroleum and / or petroleum products. The pseudomonas aeruginosa can tolerate high-salinity environments and can effectively degrade petroleum and / or petroleum products in the environments.

The invention relates to the technical field of biologic restoration of organic matter pollution, and discloses a Pseudomonas aeruginosa and an inoculant containing the Pseudomonas aeruginosa, an application thereof, and a TNT degradation method. The Pseudomonas aeruginosa is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC NO:10842. The TNT degradation method is characterized in that the Pseudomonas aeruginosa with the preservation number of CGMCC NO:10842 and / or the inoculant containing the Pseudomonas aeruginosa with the preservation number of CGMCC NO:10842 are in contact with a TNT polluted sample to degrade TNT in the TNT polluted sample. The Pseudomonas aeruginosa has high TNT degradation ability, still has good tolerance and high degradability in high-salt environment, and plays a great role in the biological restoration process of TNT polluted soil or TNT-containing wastewater.

The invention provides compositions and fusion proteins comprising a flagellin adjuvant and a Pseudomonas aeruginosa antigen. The invention further provides pharmaceutical formulations and methods for inducing an immune response against P. aeruginosa (e.g., to prevent and / or treat P. aeruginosa infection).

The invention discloses pseudomonas aeruginosa for preventing and treating vertieillium wilt in crops and application of the pseudomonas aeruginosa. The strain is named as pseudomonas aeruginosa KK9a, with preservation number as CGMCC 11637. The invention provides an inoculant containing the pseudomonas aeruginosa. The strain of the invention has an inhibitory effect on both spore germination and mycelial growth of verticillium dahlia and the strain has an efficient antagonistic action. The strain of the invention is capable of preventing and treating crop verticillium wilt caused by the verticillium dahlia, the strain has a significant effect on preventing and treating cotton verticillium wilt and control efficacy of pot experiments reaches 86% or above; therefore, the strain has a great potential in the application to bio-pesticides.

The invention relates to a method for establishing a pseudomonas aeruginosa infectious pneumonia animal model. The method comprises the following steps: 1) deeply anesthetizing a mouse by adopting isoflurane; and 2) dripping nose of the mouse with pseudomonas aeruginosa, so that a pseudomonas aeruginosa infectious pneumonia model is established. The method provided by the invention has the advantages that the mouse can be effectively infected, and the pseudomonas aeruginosa pneumonia model is successfully established; and the mouse model after infection is stable and can be applied to evaluation of vaccines and research of protective mechanisms.

The invention relates to a preparation method and application of a pseudomonas aeruginosa outer membrane protein vaccine. The preparation method comprises the following steps: constructing an Escherichia coli strain BL21(DE)3 / pET28b-His-OprH capable of expressing pseudomonas aeruginosa outer membrane proteins, inducing the strain to express a His-OprH fusion protein by using IPTG (Isopropyl-beta-d-Thiogalactoside), purifying by using Ni-NTA and a molecular sieve, and coating the fusion protein by using DHPC (1,2-Dihexanoyl-sn-glycero-3-Phosphocholine). The protein vaccine prepared by the method disclosed by the invention can be used for immunization of mice, and is capable of producing protective effects on two pseudomonas aeruginosa strains PA14 and PA103 of different serotypes and inducing the mice to produce an IgG antibody targeting at the pseudomonas aeruginosa OprH protein. The antibody is capable of mediating phagocytosis of bone marrow macrophage on the strain PA14.

A method of and device for detecting and diagnosing Pseudomonal aeruginosa in a gaseous, liquid or solid sample, employing Lux-R-like receptor-driven reporter cells.

The invention discloses keratinase generating pseudomonas aeruginosa and an application thereof. The pseudomonas aeruginosa is collected in the CGMCC (China General Microbiological Culture Collection) on March 7th, 2016, with a collection number of CGMCC No.12185. The keratinase generating pseudomonas aeruginosa degrades a keratin-containing substance by the following steps: 1) preparing a liquid inorganic salt medium; 2) fetching a pseudomonas aeruginosa strain and the keratin-containing substance, and adding into the liquid inorganic salt medium prepared in the step 1), wherein the age of the pseudomonas aeruginosa strain is t, 4h<=t<=24h, the inoculation amount of the pseudomonas aeruginosa strain in the liquid inorganic salt culture medium is a, and 0.5%<=a<=5%; and fermenting at 27-47 DEG C at a speed of 150-220rpm to degrade the keratin-containing substance. The pseudomonas aeruginosa can efficiently degrade keratin in a liquid medium taking feather as a unique carbon-nitrogen source.

The invention relates to use of a tail fiber protein of pseudomonas aeruginosa phage for preparing a bacteria detection reagent, and belongs to the technical field of microbiological detection. The preservation number of the pseudomonas aeruginosa phage is CGMCC No. 15569. The tail fiber protein P069 of the pseudomonas aeruginosa phage is used for preparing the bacteria detection reagent. The usedisclosed by the invention has the beneficial effects that the tail fiber protein P069 capable of specifically identifying pseudomonas aeruginosa and is used as a molecular identification reagent to specifically capture the pseudomonas aeruginosa, and other analysis technologies are combined to detect whole cells of the pseudomonas aeruginosa. The molecular identification reagent has the advantages of quick detection, convenience, accuracy, specificity, sensitivity and stability, is expected to being applied to site detection and quick screening of the pseudomonas aeruginosa, and can provide apowerful technological supporting platform for detecting the pseudomonas aeruginosa in the field of clinical diagnosis, food safety, environmental detection and the like.

The invention relates to the technical field of pseudomonas aeruginosa detection and discloses a pseudomonas aeruginosa chromogenic culture medium and a method for quick detection by the same. The pseudomonas aeruginosa chromogenic culture medium comprises a basic medium, a chromogenic substrate and an antibacterial agent. The chromogenic substrate comprises 2,3,5-phenyl tetrazolium chloride, 4-methyl umbelliferone-D-glucuronide, and the antibacterial agent comprises nalidixic acid, cycloserine and bile salt. 2,3,5-phenyl tetrazolium chloride acts on a pseudomonas aeruginosa metabolite to showa red color; 4-methyl umbelliferone-D-glucuronide acts on pseudomonas aeruginosa to show fluorescence under a 366nm ultraviolet lamp; by joint action of 2,3,5-phenyl tetrazolium chloride and the antibacterial agent, growth of common disturbance bacteria can be inhibited, and false positive results are avoided. By adoption of the pseudomonas aeruginosa quick detection method, detection and count of pseudomonas aeruginosa can be finished in 24h, and time saving, labor saving, stability, reliability and high sensitivity are realized.

The invention provides a streptococcus, which is a new species of streptococcus, is named as Streptococcus zengyii sp.nov.HTS25, and is registered and preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC 15344. The Streptococcus zengyii sp.nov.HTS25 is cultured in a variety of fermentation media, and can generate strong bacteriostatic active substances, also has obvious bacteriostatic effect on pseudomonas aeruginosa and salmonella typhi, also has broad development space in control of diseases caused by pseudomonas aeruginosa and salmonella typhi, and has very good development and application prospects in antibacterial agents.

The invention relates to the field of biotechnology, in particular to a screening method for aptamers and an aptamer specifically bond with pseudomonas aeruginosa. The aptamer provided by the invention has good specificity and sensitivity, a biosensor prepared by using the aptamer can realize accurate detection for pseudomonas aeruginosa, the detection range is 10<1> CFU / ml to 10<7> CFU / ml, the limit of detection is as low as 9 CFU / ul, and the aptamer has good detection capacity in the detection of the pollutants such as pseudomonas aeruginosa in a beverage.

The invention relates to a building method of attenuated pseudomonas aeruginosa and application of the attenuated pseudomonas aeruginosa to protein transfection. The building method is characterized in that glutamate racemase gene murI participating in cell wall peptidoglycan synthesis in a pseudomonas aeruginosa Delta8 bacterial strain is deleted, so that a D-glutamate nutritional deficiency typebacterial strain Delta9 is obtained. According to the application, the Delta9 bacterial strain has no cytotoxicity; the complete III type secretory system (T3SS) is reserved; foreign protein is effectively injected into mammalian cells through T3SS and can be applied to mammalian cell protein transfection. The Delta9 cannot grow in culture environment lacking D-glutamate, so that after the transfection, the bacterium is automatically cleared through D-glutamate nutrient limitation. The in vitro and in vivo application safety of the bacterium is interpreted by using HeLa cells and a mouse infection model. The positive significance is realized for developing the safe and efficient mammalian cell protein transfection technology.

The invention relates to the field of biotechnology, in particular to strains, application of the strains, a vaccine and a preparation method of the vaccine, and provides a combined inactivated vaccine for mink parvoviral enteritis and hemorrhagic pneumonia and a preparation process of the combined inactivated vaccine. The (bacterium) virus strains of the vaccine include the mink enteritis parvovirus strain (MEVB strain) and the mink pseudomonas aeruginosa G-type DL15 strain, B-type JL08 strain, C-type WD01 strain, I-type WF05 strain and F-type DL03 strain which are popular in China. The parvoviral enteritis strain and the pseudomonas aeruginosa strains serving as antigens are cultured, inactivated with formaldehyde and then mixed in proportion, aluminium hydroxide colloid is added in proportion, and the vaccine is prepared. The immunity period of the vaccine is 6 months, the immunity protection rate is 80% or above, and the vaccine can be used for preventing mink parvoviral enteritis and mink hemorrhagic pneumonia at the same time, reduces the immunization workload of raising households and the stress response of minks and has good market application prospects.

The invention provides a pseudomonas aeruginosa resistant Magainin peptide modifier and a preparation method thereof. The Magainin peptide can be natural peptide Magainin I or Magainin II; and according to a sequence from C-terminal to N-terminal, the glycine in the 23rd position of the Magainin peptide is connected to 6-(4-morpholine-1,8-naphthalimide)-lysine of the sequence K(nph)KK2(beta-Ala-NH2)4, namely M-MGN-I or M-MGN-II. The provided two novel Magainin peptide modifiers (M-MGN-I and M-MGN-II) both have an excellent broad-spectrum antibacterial effect, especially have an good antibacterial effect on infected cells or pseudomonas aeruginosa in living organisms, and are capable of removing pseudomonas aeruginosa in A549 cells and HBE cells specifically.

The invention discloses an application of three stilbene compounds, namely resveratrol, piceatannol and oxyresveratrol, in inhibiting a pseudomonas aeruginosa quorum sensing system. Pseudomonas aeruginosa is one of the most common opportunistic pathogens which can cause serious acquired infection, and the quorum sensing system of the pseudomonas aeruginosa can regulate and control the generation of various virulence factors. In a sub-lethal concentration, the three stilbene compounds, namely the resveratrol, the piceatannol and the oxyresveratrol, can significantly inhibit the swarming of the pseudomonas aeruginosa and the generation of the virulence factor, namely pyocyanine, under the precondition of avoiding influence on the growth of the bacterium (the pseudomonas aeruginosa). The three stilbene compounds, namely the resveratrol, the piceatannol and the oxyresveratrol, can be prepared into pseudomonas aeruginosa quorum sensing inhibitors so as to effectively inhibit quorum sensing and prevent bacterium infection; and the inhibitors have an important practical value in clinical bacteriostatic application.

The invention discloses a pseudomonas aeruginosa and sphingomonas paucimobilis inactivating method based on a water supply network growth ring and relates to a method for inactivating two kinds of pathogenic bacteria, namely pseudomonas aeruginosa and sphingomonas paucimobilis in drinking water. The method aims to solve the problem that an existing method for inactivating pseudomonas aeruginosa and sphingomonas paucimobilis in drinking water is poor in effect. The method comprises the single step of adding alpha-FeOOH and H2O2 to a clean water reservoir of a city water supply plant. According to the method, the cost of H2O2 is low, alpha-FeOOH is cheap and easy to obtain, is a main constituent of the growth ring and can exist continuously in a city water supply network after being added once, only H2O2 needs to be added in the later period, the requirement for equipment is simple, energy consumption is low, and investment and operation cost are reduced. A Fenton-like body formed by alpha-FeOOH and H2O2 can inactivate pseudomonas aeruginosa and sphingomonas paucimobilis existing in filtered water remarkably.

The invention discloses pseudomonas aeruginosa and application of pseudomonas aeruginosa in preparing antibacterial drugs. The preservation number of pseudomonas aeruginosa No. 31 is CCTCC No. M2013697. The pseudomonas aeruginosa No. 31 has antagonism, so that the pseudomonas aeruginosa No. 31 can be used for preparing preparation for preventing and controlling aquatic vibrio; the preparation for preventing and controlling aquatic vibrio can be used for beneficial microbial preparation to replace chemical medicine, improves water quality, and eliminates aquatic vibrio in an aquatic product cultivation system, so that damage to aquatic product cultivation objects from aquatic vibrio such as assistant hemolysis vibrio or vibrio alginolyticus is prevented, the development of the aquatic product cultivation industry is promoted, aquatic products are prevented from carrying the vibrios, and health and safety of human beings are ensured.