Method for degrading petroleum by pseudomonas aeruginosa having high rhamnolipid yield

A technology of Pseudomonas aeruginosa and rhamnolipid, which is applied in the field of marine pollution treatment, can solve the problems of high price of microbial substrates, high cost, low yield and the like, and achieves saving experiment cost and time, low cost and low cost Effect

Inactive Publication Date: 2017-07-25
OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Microbes degrade petroleum by producing biosurfactants, but due to the high price of microbial substrates, high cost of

Method used

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  • Method for degrading petroleum by pseudomonas aeruginosa having high rhamnolipid yield
  • Method for degrading petroleum by pseudomonas aeruginosa having high rhamnolipid yield
  • Method for degrading petroleum by pseudomonas aeruginosa having high rhamnolipid yield

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Experimental program
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Embodiment

[0039] 1. Screening and identification of Pseudomonas aeruginosa ZS1

[0040] 1. Dissolve 0.1g of oily sludge from the sea area of ​​Zhoushan in 1L of basal culture medium, culture it on a shaking table at 30°C and 180rpm for 3 days to obtain liquid strains, take 1mL of liquid strains and inoculate 1L of liquid culture medium, and inoculate at 30°C 180rpm rotating speed shaker culture for 50 hours to obtain bacterial liquid.

[0041] The basic medium formula is: Na 2 HPO 4 0.6g / L, KH 2 PO 4 0.2g / L, NaNO 3 4.0g / L, MgSO 4 0.3g / L, CaCl 2 0.01g / L, FeSO 4 0.01g / L, water balance.

[0042] The liquid medium formula is: glucose 20g / L, Na 2 HPO 4 0.6g / L, KH 2 PO 4 0.2g / L, NaNO 3 4.0g / L, MgSO 4 0.3g / L, CaCl 2 0.01g / L, FeSO 4 0.01g / L, water balance.

[0043] 2. Spread the bacterial liquid on the solid medium, incubate at 30°C for 12 hours, randomly select 7 colonies and inoculate them into 1L of liquid medium, culture on a shaker at 30°C and 180rpm for 50 hou...

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Abstract

The invention discloses a method for degrading petroleum by pseudomonas aeruginosa having a high rhamnolipid yield. The method comprises (1) acquisition of pseudomonas aeruginosa having a high rhamnolipid yield, (2) enlarge cultivation: inoculating a liquid medium with the pseudomonas aeruginosa obtained by the step 1, carrying out culture under shake culture conditions of 30+/-1 DEG C and 150-180 rpm for 50-60h, carrying out centrifugation and filtration, collecting mycelia and drying the mycelia to obtain bacterial powder, and (3) degradation of petroleum: putting the bacterial powder obtained by the step 2 to the sea surface polluted by petroleum according to a ratio of the bacterial powder to the petroleum of 20-30g: 100g and carrying out natural degradation for 15-30 days. The method has the advantages of fast degradation rate, no residual hazard and low cost.

Description

technical field [0001] The invention relates to the technical field of marine pollution treatment, in particular to a method for degrading petroleum by using high-yielding rhamnolipid-producing Pseudomonas aeruginosa. Background technique [0002] Oil pollution is often related to oil industry accidents, tank and pipeline transportation, leakage of oil storage tanks, etc. According to statistics, at least 400,000 tons of crude oil leak into the marine environment every year. In addition to the oil spills caused by these maritime accidents, the development of offshore oil fields is also an important source of pollution. With the growth of the global economy, global trade is becoming more and more intense, maritime transportation will become more and more frequent, and there will be more and more maritime accidents, and offshore oil pollution will definitely intensify. The use of biological methods to remediate oil-contaminated environments has attracted more and more attenti...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20C12N1/02C12R1/385C02F101/32C02F103/08
CPCC02F3/34C02F2101/32C02F2103/08C12N1/02C12N1/20
Inventor 刘建华何靖程涛梁冀北胡兴翠
Owner OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
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