60 results about "Marine oil pollution" patented technology

The invention relates to the technical field of marine environment management, in particular to an offshore oil degrading microbial inoculum and a preparation method thereof. The invention discloses an offshore oil degrading microbial inoculum, which comprises a petroleum degrading flora and a biological fixation carrier, and is characterized in that the microbial inoculum is prepared by attaching the petroleum degrading flora on the floating degradable biological fixation carrier. According to the invention, the biological fixation carrier takes an expanding degradable material as a core, and an outer layer is coated with a degradable composite membrane with positive electricity; the biological fixation carrier has floating property through the expanding degradable material; the degradable composite membrane with positive electricity and a thallus with negative electricity can attract mutually, so that the bacterium are firmly attached on the carrier, and the composite membrane is a porous loose structure, so that the degrading flora can be attached on the wall of the porous structure of the composite membrane; in comparison with the simple petroleum degrading flora, the degrading efficiency of the degrading microbial inoculum is remarkably improved. The offshore oil degrading microbial inoculum, provided by the invention, is expected to be used for bio-remediation of marine oil pollution.

The present invention discloses a petroleum degrading strain CN-3, which is preserved in China Center For Type Culture Collection, and the accession number is CCTCC M 2015537. A screening medium and a degradation medium used in experiments both use petroleum as a sole carbon source and sole energy, and the screened-out CN-3 strain is highly efficient in petroleum degradation. The petroleum degrading strain CN-3 has good petroleum degradation effect in the neutral to slightly alkaline environment, can withstand high salinity environment, and is especially suitable for the marine environment, so that the range of application of the petroleum degrading strain CN-3 in marine oil pollution treatment is expanded, and the petroleum degrading strain CN-3 has more application prospects.

The invention belongs to the technical field of bioremediation of marine oil pollution, and relates to an immobilized algal-bacterial symbiotic system for processing petroleum pollutants. The immobilized algal-bacterial symbiotic system is prepared by the following steps: (1) selection and axenic cultivation of algae species, firstly carrying out lysozyme and sodium dodecyl sulfate (SDS) sterile pretreatment on chlorella algae fluid, and then inoculating into a BG11 culture solution containing antibiotics to carry out sterile cultivation, so as to obtain sterile chlorella algae fluid for later use; (2) selection and cultivation of petroleum degrading bacteria; (3) preparation of composite carrier bacteria algae gel balls, namely immobilizing the sterile chlorella algae fluid obtained in the step (1) and the petroleum degrading bacteria obtained in the step (2) by adopting sodium alga acid and polyvinyl acetate (PVA) as immobilized carriers, and finally obtaining the immobilized algal-bacterial symbiotic system. The immobilized algal-bacterial symbiotic system can float on the water to carry out degradation of petroleum pollution, and is free of secondary pollution, free of toxic action on microorganisms, rapid in reaction speed, stable and reliable in operation, less in microorganism loss, and free of separation.

The invention provides a method for screening and domesticating marine oil pollutant microorganisms, which comprises: acquiring a microorganism source sample in a marine oil polluted area; performing enrichment culture of one generation, second generation and third generation to obtain a culture solution; screening, enriching and performing enrichment culture of one generation, second generation and third generation, obtaining an enriched culture solution; and adding nutritional materials into a designed device to domesticating the microorganisms and obtaining domesticated microorganisms. Thescreened microorganisms can be used for degrading oil pollutant in areas where marine oil pollution accidents happen, and can be for biological remediation of oil overflowing coastal beach and petroleum degradation. The method has the characteristics of high speed, economy, high efficiency and environment friendliness, can be used quickly for handling marine oil pollution accidents and is an ideal choice for screening and domesticating marine oil pollutant microorganisms.

The invention relates to a preparation method for an embedding floating bacteria agent of nutritive salt, expandable graphite and microorganism together. The preparation method for the embedding floating bacteria agent of the nutritive salt, the expandable graphite and the microorganism together comprises the steps of using a sodium alginate solution as an embedding frame, adding a nutritive salt solution, the expandable graphite and petroleum degrading bacteria to the sodium alginate solution, mixing evenly, dripping into a calcium chloride solution to embed, crosslinking for the night under the condition of 4 DEG C, and obtaining an embedding bacteria agent capable of strengthening bioremediation effect. The bacteria agent prepared by the preparation method for the embedding floating bacteria agent of the nutritive salt, the expandable graphite and the microorganism together is suitable for in-situ remediation of marine petroleum pollution, and has the advantages of being long in floating time, good in mass transfer performance, high in petroleum degrading efficiency, and the like. The method that the nutritive salt and bacteria solutions are embedded together is adopted by the embedding bacteria agent, defects that nutrition of marine environment is poor and additionally the nutritive salt is diluted quickly are overcome, and floating of the bacteria agent is achieved by addition of the expandable graphite. When the embedded bacteria agent is used, the embedded bacteria agent can be directly added to the surface of a polluted water body, effective contact with petroleum pollutant is ensured, biodegradation efficiency is effectively improved, and recycling is facilitated, and risk of a secondary pollution does not exist.

The invention relates to the technical field of marine oil pollution treatment, in particular to an air blocking oil fencing device. The air blocking oil fencing device comprises an oil fence mechanism and a guide mechanism. The oil fence mechanism comprises an oil fence floating body, a skirt body connected to the lower end of the oil fence floating body, and an air supply component connected with the skirt body. The guide mechanism is arranged at the upper end of the oil fence floating body and comprises a plurality of fixed rods fixedly connected with the upper end of the oil fence floating body, and fan blade assemblies rotationally connected with the fixed rods. By means of the air blocking oil fencing device, the air oil fencing effect can be improved, and the wave riding property of the air blocking oil fencing device can be enhanced.

The invention discloses a method for degrading petroleum by pseudomonas aeruginosa having a high rhamnolipid yield. The method comprises (1) acquisition of pseudomonas aeruginosa having a high rhamnolipid yield, (2) enlarge cultivation: inoculating a liquid medium with the pseudomonas aeruginosa obtained by the step 1, carrying out culture under shake culture conditions of 30+ / -1 DEG C and 150-180 rpm for 50-60h, carrying out centrifugation and filtration, collecting mycelia and drying the mycelia to obtain bacterial powder, and (3) degradation of petroleum: putting the bacterial powder obtained by the step 2 to the sea surface polluted by petroleum according to a ratio of the bacterial powder to the petroleum of 20-30g: 100g and carrying out natural degradation for 15-30 days. The method has the advantages of fast degradation rate, no residual hazard and low cost.

The invention discloses pseudoalteromonas sp.QN-1 with a low-temperature petroleum degradation function, and the collection number is CGMCC No. 11635. The pseudoalteromonas is selected from petroleum-polluted seas in north China, can degrade petroleum at a low temperature, and particularly, can degrade naphthalene in the petroleum at the extreme temperature of 0 DEG C. The pseudoalteromonas QN-1 can be applied to biological remediation of ocean petroleum pollution, and particularly, can be applied to naphthalene degradation in low-temperature environments, such as in winter (including glacial periods), at seas in north China.

The invention discloses a method for fixing oil decomposing bacteria by polyurethane foam and relates to fixation of a group of oil decomposing bacteria. The method for fixing oil decomposing bacteria by polyurethane foam can increase the oil decomposition rate. The method comprises the following steps of: pelletizing the polyurethane foam, boiling the polyurethane foam in boiled water, removing polyether type substances which are contained in the polyurethane foam and harmful to cells, drying the polyurethane foam, sterilizing the polyurethane foam together with a 2216 culture medium, and inoculating the polyurethane foam in the oil decomposing bacteria for shake-flask culture so that the oil decomposing bacteria enters or is adsorbed on the inner wall of the polyurethane foam to grow in order to form polyurethane foam with high-density oil decomposing bacteria. According to the invention, polyurethane foam with certain density and size, free of tox and pollution, good in mass transfer effect and energy transfer effect and also convenient and low-cost is adopted to fix the oil decomposing bacteria, so that the application performance in actual sea oil pollution abatement is remarkably improved, and the used polyurethane foam can be recycled after special treatment, therefore good economic benefit is achieved.

The present invention relates to a method for screening and immobilizing efficient petroleum hydrocarbon degradation flora, wherein the method is applicable for offshore petroleum pollution environment remediation. According to the method, a lot of petroleum hydrocarbon degradation bacterial are enriched, acclimatized and separated from petroleum pollution sea area sediments, the n (n is more than or equal to 3) strains of the bacterial with strong growth activity, different colony morphologies and different cell morphologies are selected, a petroleum hydrocarbon degradation rate of the single strain of the bacterial is determined, 16SrDNA molecule identification is performed on the bacterial, the n strains of the petroleum hydrocarbon degradation bacterial are subjected to mixing culture, the (n-1) strains of the bacterial are randomly selected from the n strains of the bacterial, and are combined to form n groups of different combinations to carry out mixing culture, the optimal combination of the petroleum hydrocarbon degradation bacterial is determined, and the petroleum hydrocarbon degradation flora with the optimal combination is prepared into immobilized bacterial balls to be stored so as to provide a spare use for offshore petroleum pollution environment remediation. The method is an efficient petroleum hydrocarbon degradation flora screening method with characteristics of science, rationality, simplness, feasibility, and meeting of optimal combination principle, wherein the efficient petroleum hydrocarbon degradation flora can be immobilizedly embedded into the ball shape, petroleum pollution impact can be buffered, and petroleum pollution biodegradation efficiency can be increased.

The invention discloses a microorganism composite bacterial preparation, and a preparation method and applications thereof, and belongs to the field of environment microorganism. The microorganism main component of the microorganism composite bacterial preparation comprises Alcanivorax dieselolei, Pseudomonas pachastrellae, Acinetobacter venetianus, and Marinobacter hydrocarbonoclasticus; the microorganism composite bacterial preparation can be used for degrading petroleum hydrocarbons, and degrading petroleum hydrocarbons in sewage, is capable of realizing comprehensive effective degradationof the large amounts of straight chain alkanes, branched-chain hydrocarbons, and aromatic hydrocarbons in petroleum hydrocarbons, is high in removing rate, is capable of solving a sea petroleum pollution pollution environment problem through degradation of petroleum hydrocarbons. The microorganism composite bacterial preparation is prepared via inoculation of a bacterial preparation culture mediumwith the Alcanivorax dieselolei, Pseudomonas pachastrellae, Acinetobacter venetianus, and Marinobacter hydrocarbonoclasticus, and co-culturing; the preparation process is simple; equipment requirements are low; operation is simple; needed environment conditions are mild; and industrial popularization and applications are convenient to realize.

The invention relates to the technical field of marine environment petroleum pollution control, in particular to an efficient screening method of marine petroleum degrading bacteria and preparation and application of a composite microbial agent. The composite microbial agent comprises mixed strains of Pseudoxanthomonas sp. S1-2, Acinetobacter sp. HC8-3S and Dietzia sp. CN-3. The marine petroleum degrading composite microbial agent has a better degradation effect on petroleum in a neutral alkaline environment, can resist to a high-salt environment, is particularly adaptive to the marine environment, and expands the application range and prospect of the petroleum degrading composite microbial agent in the marine petroleum pollution control.

The invention provides a Exiguobacterium marinum of which the collection number is CGMCC No.8841. The invention also provides a microbial inoculant which comprises a culture medium and a thallus, wherein the thallus comprises the Exiguobacterium marinum of which the collection number is CGMCC No.8841. The invention also provides application of the Exiguobacterium marinum and the microbial inoculant in degradation of petroleum hydrocarbons. The technical scheme of the invention can enhance the ocean petroleum pollution treatment efficiency more effectively.

The invention provides bacillus aerophilus with collection number of CGMCC No.8840. The invention also provides a microbial agent. The microbial agent comprises culture media and a thallus, wherein the thallus comprises bacillus aerophilus with collection number of CGMCC No.8840. The invention also provides applications of bacillus aerophilus and the microbial agent to degrading petroleum hydrocarbon. The marine petroleum pollution control efficiency can be more effectively improved by adopting bacillus aerophilus and the microbial agent.

The invention relates to an embedded type device for monitoring marine petroleum pollution in real time and belongs to the field of embedded type safety monitoring. The embedded type device for monitoring marine petroleum pollution in real time comprises a petroleum concentration detection head, a signal amplification circuit I and the like; a storage battery camera is connected with a controllable rotating bracket and is fixed on a gunwale in the front of a ship body; a degrading agent discharge opening penetrates through the ship body to be connected with a petroleum degrading agent storagecabin, and is arranged behind the ship body; an electromagnetic valve is arranged at the tail end of the degrading agent discharge opening; a control chamber is arranged behind the ship body and is insulated from the outside; and the signal amplification circuit I, a storage battery, a singlechip control module, a direct current motor I, a relay control circuit, a wireless communication module andan electromagnetic valve control circuit are arranged in the control chamber. The embedded type device for monitoring marine petroleum pollution in real time is simple in structure and low in cost, can monitor the petroleum pollution condition of each passing place in real time, and can treat floating petroleum in time. When the petroleum concentration of the certain passing place of the ocean bythe device exceeds a set threshold value, the device gives alarm to a user terminal.

The invention provides a method for screening and domesticating marine oil pollutant microorganisms, which comprises: acquiring a microorganism source sample in a marine oil polluted area; performing enrichment culture of one generation, second generation and third generation to obtain a culture solution; screening, enriching and performing enrichment culture of one generation, second generation and third generation, obtaining an enriched culture solution; and adding nutritional materials into a designed device to domesticating the microorganisms and obtaining domesticated microorganisms. The screened microorganisms can be used for degrading oil pollutant in areas where marine oil pollution accidents happen, and can be for biological remediation of oil overflowing coastal beach and petroleum degradation. The method has the characteristics of high speed, economy, high efficiency and environment friendliness, can be used quickly for handling marine oil pollution accidents and is an ideal choice for screening and domesticating marine oil pollutant microorganisms.

The invention discloses Pseudoalteromonas sp.DC-2 having a low-temperature petroleum degrading function and collected under CGMCC No.11633. The Pseudoalteromonas sp.DC-2 is screened from a sea area polluted by petroleum in North China, can efficiently degrade petroleum at low temperature, especially in extreme environment of 0 DEG C. The Pseudoalteromonas sp.DC-2 can be applied in bioremediation of sea petroleum pollution, especially in petroleum spilling bioremediation at low temperature in the sea area in North China in winter (including glacial period).

The invention provides a marine oil pollution boom, which belongs to the technical field of decontamination equipment. The utility model solves the problem that the existing oil boom is inconvenient to use. The offshore oil pollution containment boom includes several inflatable strip-shaped bags, several of which are arranged horizontally and transversely in turn, and two adjacent bags are connected by reinforcing cloth, and the lower edge of the reinforcing cloth is fixed. There are two rod joints connected, and the two rod joints are connected, and the two rod joints can swing relatively. The bottom of the bag body is also provided with a counterweight bar. The left end of the counterweight bar and the The rod joint on the reinforcing cloth is detachably connected, and the right end of the counterweight rod is detachably connected to the rod joint on the reinforcing cloth on the right side of the counterweight rod. The offshore oil pollution boom is easy to use and has a stable structure.

The invention discloses marine oil pollution treating equipment aims at overcoming the defect that an existing overflow oil collection and treating device cannot thoroughly collect and treat sea surface overflow oil. The marine oil pollution treating equipment comprises a floating body. The floating body is provided with an oil passing groove which runs through the front end of the floating body. The two sides of the oil passing groove at the front end of the floating body are each connected with an oil guide belt. The two oil guide belts are arranged in an inverted V shape. The front end of the oil passing groove is provided with a primary oil collection mechanism which comprises a primary oil groove and oil scooping discs. The two sides of the primary oil groove are each provided with an installation shaft. The installation shafts are in fastened connection with the floating body. Each installation shaft is rotationally sleeved with a rotating sleeve driven by a motor. The axes of the shaft sleeves are horizontally set. Each rotating sleeve is evenly provided with a plurality of supporting rods in a connected mode, and the supporting rods on the two rotating sleeves are arranged in a one-to-one opposite mode. The ends of the supporting rods are provided with supports. The supports are provided with arc balance sliding grooves concaved inwards. The lower surfaces of the two ends of each oil scooping disc are provided with semi-circular balance bases. The lower surfaces of the balance bases on the same oil scooping disc are in sliding connection with the interiors of the balance sliding grooves of the two corresponding oppositely-arranged supporting rods.

The invention discloses a self-cleaning sphere for cleaning marine oil pollution, and belongs to the field of marine oil pollution cleaning devices. When the self-cleaning sphere for cleaning the marine oil pollution is put into a sea area polluted by petroleum, seawater mixed with the oil pollution can enter an inner cavity of an outer spherical shell through spherical shell through holes, and various dissolved solutes and oil pollution in the seawater cannot enter the seawater through semipermeable membrane spherical sleeve, so that the various solutes and oil pollution are greatly gatheredin filled fiber. Purified water entering the semipermeable membrane spherical sleeve can dissolve sodium chloride crystalline in the semipermeable membrane spherical sleeve to form a high-concentration sodium chloride solution, because the inner-side hydraulic pressure and the outer-side hydraulic pressure of the semipermeable membrane spherical sleeve are different, the oil pollution and the seawater can be continuously sucked into the outer spherical shell and the semipermeable membrane spherical sleeve, the situation that the auxiliary operation is carried out in crude oil leakage areas bytechnical staff can be avoided, the technical staff can be prevented from being eroded by crude oil in the oil pollution cleaning work process, and the technical staff is not prone to being seriouslythreatened.

The invention belongs to the field of marine pollution degradation, and particularly relates to a repairing method for degrading marine petroleum pollution. After a large number of functional microorganisms are cultivated in a fermental manner, a bacteria solution is adsorbed and fixed on zeolite by using an immobilization technology and is wrapped in a PGA packaging mode to form a microbial agent product, a protecting agent (nutritional agent) wraps the surface of the zeolite, in a settlement process, loss of the microorganisms in apertures of the zeolite is reduced as much as possible, meanwhile, a wrapping agent serves as a nutrient source of the microorganisms, and secondary pollution to water is avoided. The throwing mode is a brand-new throwing line, throwing coverage wide reaches 9 meters, microbial agents are spaced from one another uniformly, throwing efficiency is high, and by the high-quality microbial agents and accurate throwing, the efficient repairing method for degrading marine petroleum pollution is formed.

The invention discloses a marine oil pollution recovery device, and relates to an oil pollution recovery device. The marine oil pollution recovery device comprises a horizontal floating plate, whereinan upper part of the horizontal floating plate is fixedly connected with an installation frame, a right side below the horizontal floating plate is fixedly connected with a connecting column, a bottom of the connecting column is fixedly connected with a cover body, a right side of the cover body is fixedly connected with an arc-shaped connecting pipe, a tail end of the arc-shaped connecting pipeis fixedly connected with a guide sleeve, an inner side of the guide sleeve is slidingly connected with an oil collecting funnel, an the outer side of the oil collecting funnel is fixedly connected with an annular airbag, a top of the installation frame is fixedly connected with a motor, an output end of the motor is fixedly connected with a cam, a right side above the horizontal floating plate isfixedly connected with an inflator, and the inflator is matched with the cam. When using, oil can be separated from seawater completely for twice, so the oil pollution floating on the surface of thesea can be recovered, the separation effect is good, the oil pollution can be separated well, the seawater is avoided being polluted seriously, and adequate protection is provided to the leakage of marine oil pollution.

The present invention discloses a petroleum degrading strain CN-3, which is preserved in China Center For Type Culture Collection, and the accession number is CCTCC M 2015537. A screening medium and a degradation medium used in experiments both use petroleum as a sole carbon source and sole energy, and the screened-out CN-3 strain is highly efficient in petroleum degradation. The petroleum degrading strain CN-3 has good petroleum degradation effect in the neutral to slightly alkaline environment, can withstand high salinity environment, and is especially suitable for the marine environment, so that the range of application of the petroleum degrading strain CN-3 in marine oil pollution treatment is expanded, and the petroleum degrading strain CN-3 has more application prospects.

The invention provides a rapid recovery device for offshore oil pollution. The rapid recovery device comprises a ship body, the ship body is connected with an oil storage bin and connected with a scraper through a lifting mechanism, the scraper is obliquely arranged, the front end of the scraper is used for collecting floating oil dirt, and an oil collecting groove is formed in the rear end of the scraper and communicates with the oil storage bin through an oil pump. The emulsion breaker is suspended in an emulsion layer through specific gravity adjustment, so that the emulsion layer is separated into oil and water. An emulsion layer is broken into oil and water, and the problem that only an oil layer can be collected in the market is solved. The scraping plate inclines relative to the water surface, so that when the upstream end of the scraping plate is located in oil dirt, the ship body operates at a low speed, and the oil dirt can flush the scraping plate to enter the oil collecting groove. The top overflow position of the overflow groove is lower than the top of the oil storage bin. The water overflows and is discharged from the top of the overflow groove under the action of gravity until the storage cabin is full of the oil dirt, the content of the water mixed in the oil storage cabin is reduced, the oil dirt water removal procedure in the later stage of oil dirt collection is reduced, and the oil dirt collection efficiency of the whole structure is improved.

The invention relates to a loofah complex oil absorb material. The preparation process is free from harmful and toxic pollutant and has the advantages of simple operation and high efficiency. Loofah is combined with grapheme, so as to obtain the complex oil absorb material with large pore structure and good air permeability, and can be reused for many times. The loofah complex oil absorb materialprepared by the invention has high oil absorbing property and reuse effect, and can be applied to marine oil pollution treatment and oil fume exhaust gas purification.

The invention belongs to the technical field of pollutant degradation equipment, and particularly relates to a microorganism hosting type degradation device for an offshore oil pollution area. The device comprises a plurality of degradation units, each degradation unit comprises a floating disc and a cleaning device, and the floating discs of every two adjacent degradation units are connected through a connecting pipe; the cleaning device comprises a shell, an oil absorption layer, a living ball and a connecting pipe; and a plurality of meshes are formed in the shell, the oil absorption layer is arranged in the shell, a distance is reserved between the oil absorption layer and the shell, a plurality of screen holes are formed in the oil absorption layer, the living ball is located on the inner wall of the oil absorption layer and attached to the inner wall of the oil absorption layer, microorganisms capable of degrading petroleum are loaded on the living ball, one end of the connecting pipe is connected to the living ball, and the other end sequentially penetrates through the oil absorption layer and the shell and is finally connected with the floating disc. The device provided by the invention provides a relatively stable living place for survival of microorganisms and degradation of petroleum, prevents the microorganisms from being dispersed by seawater, and improves the petroleum degradation effect.