The present invention relates to a method for screening and immobilizing efficient petroleum hydrocarbon degradation flora, wherein the method is applicable for offshore petroleum pollution environment remediation. According to the method, a lot of petroleum hydrocarbon degradation bacterial are enriched, acclimatized and separated from petroleum pollution sea area sediments, the n (n is more than or equal to 3) strains of the bacterial with strong growth activity, different colony morphologies and different cell morphologies are selected, a petroleum hydrocarbon degradation rate of the single strain of the bacterial is determined, 16SrDNA molecule identification is performed on the bacterial, the n strains of the petroleum hydrocarbon degradation bacterial are subjected to mixing culture, the (n-1) strains of the bacterial are randomly selected from the n strains of the bacterial, and are combined to form n groups of different combinations to carry out mixing culture, the optimal combination of the petroleum hydrocarbon degradation bacterial is determined, and the petroleum hydrocarbon degradation flora with the optimal combination is prepared into immobilized bacterial balls to be stored so as to provide a spare use for offshore petroleum pollution environment remediation. The method is an efficient petroleum hydrocarbon degradation flora screening method with characteristics of science, rationality, simplness, feasibility, and meeting of optimal combination principle, wherein the efficient petroleum hydrocarbon degradation flora can be immobilizedly embedded into the ball shape, petroleum pollution impact can be buffered, and petroleum pollution biodegradation efficiency can be increased.