Repairing method for degrading marine petroleum pollution
A remediation method, offshore oil technology, applied in microorganism-based methods, polluted waterway/lake/pond/river treatment, chemical instruments and methods, etc., can solve secondary pollution, cannot form long-term effects, remediation technology It can only be used for a short time to achieve the effect of timely and effective communication, conducive to ecological restoration, and ensuring safety and efficiency.
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[0035] A restoration method for marine oil pollution degradation, comprising the following steps:
[0036] (1) Preparation of seed solution
[0037] a. Preparation of medium: Add 10g of tryptone, 5g of yeast extract and 10g of sodium chloride into 950ml of deionized water, shake the container until the solute is dissolved, adjust the pH to 7.0 with 5mol / L NaOH solution, and then set the pH with deionized water. Make up to 1L, steam sterilize at 15psi high pressure for 21min;
[0038] b. Inoculate marine restoration bacteria into the culture medium in step a, and place it on a shaker to cultivate until the absorbance is greater than 1.0, and there is no miscellaneous bacteria in the microscopic examination to prepare the seed liquid;
[0039] (2) Preparation of bacterial liquid
[0040] a, primary fermentation: in the primary fermentation tank, 450g of tryptone, 120g of yeast extract powder, 1.4g of calcium chloride, 14g of magnesium sulfate, 800g of sodium chloride, 4g of fe...
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