The invention relates the method for preparing rich silver antibiotic film used for medical pyrolytic carbon and TiN film. It solves the antibiosis question. The method comprises the following steps: on the pyrolytic carbon: a buffing and cleaning; b evacuating; c sputtering and cleaning for 5 minutes with 1keV nitrogen ion; d injecting silver ion: 70, 30keV energy, 2-8mA beam current, 2X1014/cm2-3X1014/cm2 dosage, and forming rich silver antibiotic film and antibiosis ratio being 100%; on TiN film: a buffing and cleaning; b evacuating; c sputtering and cleaning for 5 minutes with 1keV nitrogen ion; d sputtering Ti target with 3keV, 80mA Ar+ ion, 20-40keV, beam current: bombarding with 2-8mA high-energy N+ ion for 10-20 minutes; 50-350eV, beam current: bombarding with 10-30mA low-energy N+ ion for 60-180 minutes to deposit, N2 air pressure being 8X10 3Pa; e injecting silver ion: 30-80keV, beam current: 2-8mA, 1X1017/cm2-5X1018/cm2 dosage, forming rich silver antibiotic film and antibiosis ratio being 90%. The film has the advantages of good abradability, strong adhesive force and anti-corrosion, good cell compatibility and long antibiosis time-effect. The film can be used to prepare artificial heart valve and hard tissue alternate material which is implanted in human.