Preparation method for embedding floating bacteria agent of nutritive salt and expandable graphite and microorganism together
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A technology of expanded graphite and nutrient salts, which is applied in the field of water treatment, can solve the problems of hindering the effective contact of high-carbon hydrocarbon slick oil, the lack of nitrogen and phosphorus nutrients, and the low utilization rate of microorganisms, so that there is no risk of secondary pollution and rapid degradation , Overcoming the effect of low microbial degradation efficiency
Inactive Publication Date: 2013-03-20
TONGJI UNIV
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The method of physical restoration uses manual or mechanical means to fence and absorb the floating oil, but the main disadvantages of this technology are high cost, incomplete oil removal, and high operation requirements; the chemical restoration method is to add chemical agents or burn This method is easy to produce secondary pollution and has certain dangers; bioremediation technology and the combination technology around bioremediation will become the future marine overflow due to its safety, economy, high efficiency and no secondary pollution. The focus of oil remediation technology development
[0004] Although microbial remediation technology has obvious advantages, there are still some problems: first, oil-degrading microorganisms are affected by factors such as the living environment and competition among indigenous st
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Embodiment 1
[0018] (1) Preparation of petroleum degrading bacteria liquid
[0019] The strains of Alkanovores, Alcaligenes, Gordonella, Dietzella, Bacillus and Halomonas (the above strains can be obtained from the market) are calculated as 0.5:1:1 by weight fraction: 1.5:1.5:2, take 2g and inoculate in 50mL inorganic salt medium containing crude oil concentration of 1000mg / L, shake and culture in a constant temperature incubator at 30°C and 120 r / min for 5 days, and set aside.
[0020] Described inorganic salt culture medium is NaCl 0.5%, (NH 4 ) 2 SO 4 0.1%, MgSO 4 0.01%, NaNO 3 0.2%, KH 2 PO 4 0.4%, K 2 HPO 4 ·3H 2 O 1.0%, the rest is distilled water, pH 7.2. The sterilization condition is 0.1Mpa damp heat sterilization for 30min.
[0021] (2) Preparation of floating-type embedded bacteria agent
[0022] Add 0.2g of sodium alginate and 10mL of water to a beaker, heat and stir in a water bath at 60°C until the sodium alginate dissolves evenly, take it out and cool to room...
Embodiment 2
[0027] (1) Preparation of petroleum degrading bacteria liquid
[0028] Alkanovora, Alcaligenes, Gordonella, Dietzella, Bacillus and Halomonas (all of the above strains can be purchased from the market) are calculated as 0.5:0.5:1:1.5 by weight fraction: 2:2, take 2g and inoculate in 50mL of inorganic salt medium containing crude oil concentration of 1000mg / L, shake and culture in 30°C, 120 r / min constant temperature incubator for 5 days, and set aside.
[0029] Described inorganic salt culture medium is NaCl 0.5%, (NH 4 ) 2 SO 4 0.1%, MgSO 4 0.01%, NaNO 3 0.2%, KH 2 PO 4 0.4%, K 2 HPO 4 ·3H 2 O 1.0%, the rest is distilled water, pH 7.2. The sterilization condition is 0.1Mpa damp heat sterilization for 30min.
[0030] (2) Preparation of floating-type embedded bacteria agent
[0031] Add 0.4g of sodium alginate and 10mL of water into a beaker, heat and stir in a water bath at 80°C until the sodium alginate dissolves evenly, take it out and cool to room temperatur...
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Abstract
The invention relates to a preparation method for an embedding floating bacteria agent of nutritive salt, expandable graphite and microorganism together. The preparation method for the embedding floating bacteria agent of the nutritive salt, the expandable graphite and the microorganism together comprises the steps of using a sodium alginate solution as an embedding frame, adding a nutritive salt solution, the expandable graphite and petroleum degrading bacteria to the sodium alginate solution, mixing evenly, dripping into a calcium chloride solution to embed, crosslinking for the night under the condition of 4 DEG C, and obtaining an embedding bacteria agent capable of strengthening bioremediation effect. The bacteria agent prepared by the preparation method for the embedding floating bacteria agent of the nutritive salt, the expandable graphite and the microorganism together is suitable for in-situ remediation of marine petroleum pollution, and has the advantages of being long in floating time, good in mass transfer performance, high in petroleum degrading efficiency, and the like. The method that the nutritive salt and bacteria solutions are embedded together is adopted by the embedding bacteria agent, defects that nutrition of marine environment is poor and additionally the nutritive salt is diluted quickly are overcome, and floating of the bacteria agent is achieved by addition of the expandable graphite. When the embedded bacteria agent is used, the embedded bacteria agent can be directly added to the surface of a polluted water body, effective contact with petroleum pollutant is ensured, biodegradation efficiency is effectively improved, and recycling is facilitated, and risk of a secondary pollution does not exist.
Description
Technical field [0001] The present invention is a water treatment technology field, which involves a preparation method for consolidating floating bacteria for oil pollution water purification in situ purification of water pollution water. Background technique [0002] With the rapid development of the industry, the market's demand for oil and its derivative products has increased year by year. The development of the oil mining industry and the prosperity of the shipping industry have led to more and more petroleum hydrocarbons entering the water body each year.It has become a hot spot in the environmental field. [0003] At present, the repair technology of oil pollution mainly includes physical repair, chemical repair, biological repair, and combination repair technology.The method of physical repair uses artificial or mechanical means to fence and adsorb floating oil, but the cost, incomplete oil removal, and high operating requirements have become the main disadvantages of th...
Claims
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