The invention belongs to the technical field of microbial degradation of organic pollutants, and discloses a GY2B degrading bacteria immobilizing ball as well as a preparation method and application thereof. The GY2B degrading bacteria immobilizing ball is a gel ball formed by embedding GY2B degrading bacteria by taking a crosslinking polymerization product of polyvinyl alcohol, algin and kaolin as a carrier. The preparation method disclosed by the invention comprises the following steps: (1), sequentially adding polyvinyl alcohol and algin into water, heating, stirring and dissolving respectively, adding kaolin, and stirring and mixing uniformly so as to obtain a mixed solution; (2), cooling the mixed solution to room temperature under irradiation of ultraviolet light, adding a GY2B degrading bacteria suspension, and uniformly stirring to obtain a bacteria embedding solution; and (3), dropwise adding the bacteria embedding solution into a crosslinking solution, and crosslinking, solidifying and condensing to obtain the GY2B degrading bacteria immobilizing ball. The GY2B degrading bacteria immobilizing ball has more thorough, rapid and steady degrading effects to phenol; furthermore, a steady degrading environment can be provided; and the GY2B degrading bacteria immobilizing ball is adaptive to a toxic environment.