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Disease control strain adopting quorum sensing signal molecules AHLs as target and application thereof

A quorum sensing signal, disease control technology, applied in the direction of application, bacteria, microorganism-based methods, etc., to achieve the effect of huge application potential

Active Publication Date: 2019-02-05
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus cereus has a significant degradation effect on the quorum sensing signal molecule AHLs, and has great potential in the prevention and treatment of pathogens that rely on AHLs to cause disease, and has great practical significance for solving the problems of chemical pesticide or antibiotic abuse and pathogenic bacteria resistance

Method used

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  • Disease control strain adopting quorum sensing signal molecules AHLs as target and application thereof
  • Disease control strain adopting quorum sensing signal molecules AHLs as target and application thereof
  • Disease control strain adopting quorum sensing signal molecules AHLs as target and application thereof

Examples

Experimental program
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Embodiment 1

[0050] Isolation and screening of embodiment 1 Bacillus cereus XN-42

[0051] 1.1. Soil sample collection: Soil samples collected from the school farm of South China Agricultural University were used as microbial sources.

[0052] The soil samples were collected from the vegetable field of the school farm of South China Agricultural University in Guangzhou City, Guangdong Province on July 30, 2017. The samples were taken here, bagged, and brought back to the laboratory as a source of microorganisms for the isolation of strains.

[0053] 1.2. Enrichment culture of bacterial strains: Prepare basal salt medium (MSM), put 50mL of MSM medium in a 250mL Erlenmeyer flask, sterilize at 121°C for 20min, add OHHL mother solution (concentration: 100mm / L, the solvent is acetonitrile), so that the final concentration of OHHL in the culture medium is 5 μ mol / L, then add 5 g of soil samples, place it at 30 ° C, after 200 rpm shaker culture for 7 days, transfer the samples to the OHHL concen...

Embodiment 2

[0059] Example 2 Identification of Bacillus cereus strain XN-42

[0060] In this example, morphological characteristics and 16S rDNA phylogenetic analysis were carried out on the degrading bacteria XN-42, and the strain was identified as Bacillus cereus. details as follows:

[0061] 2.1 Morphological characteristics of colony: cultured on LB solid plate for 48 hours, the colony is light yellow, round, raised, opaque, with neat edges, such as figure 1 Shown; in LB liquid medium, it is diffuse and turbid, aerobic, and grows well at 30°C.

[0062] 2.2 Morphological characteristics of bacteria: such as figure 2 As shown, the bacteria are rod-shaped, with flagella on the surface.

[0063] 2.3 16S rDNA sequence and phylogenetic analysis: The length of the 16S rDNA gene sequence of strain XN-42 is 1436bp. After comparison with the NCBI database (http: / / www.ncbi.nlm.nih.gov / ), it was found that the strain XN- 42 has a high homology (>99%) with Bacillus cereus, and its phylogeneti...

Embodiment 3

[0064] The antibiotic susceptibility analysis of embodiment 3 strain XN-42

[0065] In order to better study the strain XN-42, this example carried out a variety of antibiotic sensitivity tests on the strain XN-42, the specific results are as follows Figure 4 shown.

[0066] The experimental results showed that the strain XN-42 had a resistance of more than 400 μg / mL to ampicillin, kanamycin, neomycin sulfate and streptomycin, a resistance of 150 μg / mL to gentamicin, and a resistance to carboxyl The resistance to benzyl penicillin reached 350 μg / mL, the resistance to tetracycline reached 10 μg / mL, and the resistance to chloramphenicol all reached below 5 μg / mL. This result can be used as a reference for subsequent research to select appropriate antibiotics.

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Abstract

The invention discloses a disease control strain adopting quorum sensing signal molecules AHLs as a target and application thereof. A preservation number of the strain is GDMCC (Guangdong Microbial Culture Collection Center) 60434. The bacillus cereus has good degradation activity for the quorum sensing signal molecules AHLs, is stable and significant in degradation effect, and has vast application potential in the aspect of preventing AHLs-mediated disease pathogenic bacteria, thereby providing a novel way and method for biological prevention and control of the disease.

Description

technical field [0001] The invention relates to a control bacterial strain, in particular to a disease control bacterial strain targeting quorum sensing signal molecule AHLs and application thereof. Background technique [0002] In agricultural production, efficient prevention and control of pathogenic bacteria and reduction of losses caused by diseases have always been the goals that producers want to achieve. Pectin bacillus (Pectobacterium) is a class of pathogenic bacteria that is harmful to agricultural production. This kind of pathogenic bacteria can secrete a variety of hydrolytic enzymes, such as: pectinase, polygalactase, protease and so on. Pectin bacteria use these hydrolytic enzymes to break the defense system of the host plant, destroy the plant tissue structure, successfully infect and cause the symptoms of soft rot in the host plant, and eventually lead to the reduction or even extinction of the relevant crops. For this type of pathogenic bacteria, there are ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/085
CPCA01N63/00C12N1/20C12N1/205C12R2001/085
Inventor 陈少华郭云帆张译尹叶田单雯艳阳芳张炼辉
Owner SOUTH CHINA AGRI UNIV
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