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Pseudoalteromonas sp.DC-2 having low-temperature petroleum degrading function and application thereof

A technology of alternating Pseudomonas and oil, applied in the field of microorganisms, can solve the problems of ecosystem hazards, slow evaporation of short-chain petroleum hydrocarbons, increase the difficulty of oil spill emergency and ecological restoration work, etc. The effect of strong hydrocarbon degradation ability

Active Publication Date: 2016-08-31
DALIAN MARITIME UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In winter, especially during the glacial period, marine oil spill accidents occur. Due to the slow evaporation of short-chain petroleum hydrocarbons and the condensation of long-chain hydrocarbons into solids, organic pollutants persist, which will cause considerable harm to the local ecosystem and increase the risk of pollution. Increased the difficulty of oil spill response and ecological restoration

Method used

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  • Pseudoalteromonas sp.DC-2 having low-temperature petroleum degrading function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Isolation and identification of bacterial strain Pseudoalteromonas sp.DC-2

[0019] 1. Isolation of strain Pseudoalteromonas sp.DC-2

[0020] (1) Sample collection: surface seawater near the Passenger Roller Wharf in Dagang District, Dalian Port;

[0021] (2) Enrichment and acclimatization: Add 100 mL of MMC medium to a 250 mL Erlenmeyer flask, sterilize at 120°C for 15 minutes, add 5 mL of collected seawater samples and 1 mL of sterilized diesel oil. Put the mixed solution into a biochemical incubator, shake and cultivate at 150r / min and 10°C for 30 days; after the cultivation, take 5 mL of the culture solution for the next round of transfer culture: add 5 mL of the culture solution to the sterilized tank containing 1 mL of diesel oil In 100mL MMC culture medium, cultured at 150r / min for 30d. Repeat the above steps, the culture temperature of each round is 1°C lower than that of the previous round, until 0°C, and acclimate at 0°C for a long time;

[0022] ...

Embodiment 2

[0026] Fermentation of Example 2 bacterial strain Pseudoalteromonas sp.DC-2

[0027] Use a sterile inoculation loop to pick up the purified bacterial lawn into a conical flask filled with 100mL of sterilized 2216E liquid medium, and culture it under low-temperature shaking for 7 days (0°C, 120r / min) to become a seed solution; The liquid was added into the 2216E liquid medium at a volume ratio of 10%, and cultured at 0° C. for 7 days to obtain a fermentation liquid.

Embodiment 3

[0028] Example 3 Determination of Degradation Rate of Petroleum Hydrocarbons of Bacterial Strain Pseudoalteromonas sp.DC-2 at 0°C

[0029] (1) Degrading bacteria seed solution

[0030] Use a sterile inoculation loop to pick up the purified bacterial lawn into a conical flask filled with 100 mL of sterilized 2216E liquid medium, and culture it with shaking at low temperature for 7 days (0°C, 120r / min).

[0031] (2) Degradation culture

[0032] Add 1 mL of diesel oil into the conical flask with 100 mL of sterilized MMC liquid medium, inoculate the seed liquid of bacterial strain Pseudoalteromonas sp. ). The culture medium not inoculated with degrading bacteria was used as the blank group.

[0033] (3) Extraction

[0034] After the degradation culture, 3 mL of 50% sulfuric acid solution was added to the culture medium for acidification. Transfer the acidified culture solution into a 250mL separatory funnel. Clean the empty Erlenmeyer flask with 20mL of n-hexane, then pour t...

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Abstract

The invention discloses Pseudoalteromonas sp.DC-2 having a low-temperature petroleum degrading function and collected under CGMCC No.11633. The Pseudoalteromonas sp.DC-2 is screened from a sea area polluted by petroleum in North China, can efficiently degrade petroleum at low temperature, especially in extreme environment of 0 DEG C. The Pseudoalteromonas sp.DC-2 can be applied in bioremediation of sea petroleum pollution, especially in petroleum spilling bioremediation at low temperature in the sea area in North China in winter (including glacial period).

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a strain of marine low-temperature bacteria and its application in bioremediation of marine oil pollution. The strain has the characteristic of being able to efficiently degrade petroleum hydrocarbons under the extreme condition of 0°C in the marine environment, and can be used for bioremediation of oil spills in ice ages in the northern seas of my country. Background technique [0002] With the rapid development of the marine transportation industry and the rapid rise of the marine mining industry, marine oil spill accidents have become increasingly frequent, causing serious damage to the balance of the marine environment. In winter, especially during the glacial period, marine oil spill accidents occur. Due to the slow evaporation of short-chain petroleum hydrocarbons and the condensation of long-chain hydrocarbons into solids, organic pollutants persist, which will cause consid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/32
CPCC02F3/344C02F2101/32C12N1/205C12R2001/38
Inventor 郭平林建国曹宾霞
Owner DALIAN MARITIME UNIVERSITY
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