The invention discloses a strain of Pseudoalteromonas marina, and a Pseudoalteromonas marina decolorization flocculant preparation method, wherein the is Pseudoalteromonas marina separated and screened from the mud discharged by Ruditapes philippinarum bred in the marine farm of Zhujiajian, Zhoushan, Zhejiang, China, and the identifying results show that the strain is Pseudoalteromonas sp.. The preparation method comprises: seed liquid culture, liquid culture medium preparation, expansion fermentation culture, and flocculant preparation. According to the present invention, the carbon source inthe culture medium is replaced with the hydrolysis liquid obtained through the crop waste hydrolysis with the inorganic acid, such that the cost is saved; the sandy soil is used as the carrier, and the bacteria are adhered to the carrier, such that the effective components of the flocculant can be simply and completely separated from the culture liquid; and the prepared decolorization flocculanthas advantages of less consumption, high decoloration rate, large floc forming, quick settling, stable performance, good decolorization flocculation effect, no potential toxicity to organisms, healthand environmental protection.