Pseudoalteromonas sp., dextran enzyme-producing method using same and dextran enzyme product

A technology of alternating Pseudomonas and dextran, which is applied in biochemical equipment and methods, enzymes, bacteria, etc., and can solve the problems of insufficient research on medicinal enzymes and insufficient research on marine-derived enzyme preparations.

Inactive Publication Date: 2011-08-17
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Although my country's enzyme preparation market is growing rapidly, the research on marine enzyme preparation is insufficient
Research on enzyme preparations in my country is mostly concentrated in food, industry, and agriculture, but research on enzymes used in medicine is insufficient

Method used

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  • Pseudoalteromonas sp., dextran enzyme-producing method using same and dextran enzyme product
  • Pseudoalteromonas sp., dextran enzyme-producing method using same and dextran enzyme product
  • Pseudoalteromonas sp., dextran enzyme-producing method using same and dextran enzyme product

Examples

Experimental program
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Effect test

Embodiment 1

[0070] Example 1. A Pseudomonas alternata LP602 ( Pseudoalteromonas sp. LP602) CGMCC N0.4319. The strain has the following characteristics: the strain is a Gram-negative rod-shaped bacterium with capsules and no spores, and its size is about 1.7-2μm×0.8-1.2μm; the colony characteristics on the solid medium containing dextran: colony diameter 4 mm-7 mm, round, milky white, moist, smooth edges, and protruding in the middle, add 95% ethanol after the colony grows, freeze at -20°C for 3~4 hours, a transparent circle appears around the colony; the oxidase is positive, and can ferment glucose , methyl red, negative V-P test, cannot grow without sodium chloride, can liquefy gelatin, cannot produce indole; can utilize glycerin, alanine, glutamic acid, pyruvic acid, but cannot utilize lactose, galactose, fructose, Cellobiose, maltose. The growth characteristics of Pseudomonas alternata LP602 are: the growth temperature range of the strain is 4-37°C, the growth pH range is 6.0-11.0...

Embodiment 2

[0071] Example 2. Pseudomonas alternata LP602 described in a kind of embodiment 1 ( Pseudoalteromonas sp. LP602) CGMCC No.4319 The method for producing dextranase, the steps are as follows: Inoculate Pseudomonas alternata LP602 strain into 2216E medium, rotate at 180r / min, fill with 20%, cultivate for 12 hours, and obtain seed solution ; Inoculate the seed solution with 2% inoculum in the fermentation medium, culture at 180r / min, 25°C for 24h, centrifuge at 10000r / min for 5min, and take the supernatant as the crude dextranase solution.

Embodiment 3

[0072] Example 3. A dextranase produced by the method described in Example 2, the dextranase has the following characteristics: the properties of the dextranase are: the suitable action temperature of the dextranase is 20°C, 5°C There is still 45% of the enzyme activity, and it has a high catalytic activity in the temperature range of 5°C to 40°C. The thermal stability of the produced dextranase is good, and the enzyme activity can still be maintained after incubation at 50°C for 2.5 hours More than 74%, the enzyme activity can still maintain 50% after incubation at 80°C for 2.5 h. Dextranase is stable in the pH range of 7.0-9.0.

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Abstract

The invention relates to a Pseudoalteromonas sp. LP602 CGMCC No.4319. The strain is characterized in that the strain is a Gram-negative rod-shaped bacterium, and has capsules but no spores; and the growth temperature range of the strain is 4-37 DEG C, the growth pH range is 6.0-11.0, and the grown NaCl concentration range is 0.5-10%. The invention also discloses a method for producing a dextran enzyme by using the Pseudoalteromonas sp. and a dextran enzyme product prepared by the method. The dextran enzyme plays an important role in the aspects of saprodontia prevention and treatment, medicaldextran production and the like.

Description

technical field [0001] The present invention relates to a kind of microorganism, especially a kind of Pseudomonas alternata LP602 ( Pseudoalteromonassp .LP602) CGMCC No.4319 (preserved in CGMCC, General Microorganism Center of China Microbiological Culture Collection Management Committee on November 10, 2010, and the preservation number is CGMCC No.4319); the present invention also relates to the strain producing dextranase methods and products. Background technique [0002] Dextran is mainly glucose linked by α-1,6-glucosidic bonds. Dextranase (Dextranase, α-D-1,6-Glucan-6-D-Glucanohydrolase, EC3.2.1.11), also known as α-glucanase, is a specific cleavage of α-1 in dextran , 6 glycosidic bond hydrolase. [0003] At present, the dextranase-producing microorganisms reported at home and abroad are mainly Penicillium ( Penicillium sp. ), Star Lipoyeast ( Lipomyces starkeyi ), Chaetomium Diagonum ( Chaetomium gracile ), Aspergillus ( Aspergillus ustus ), Bacillus ( B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/26C12N9/44
Inventor 吕明生王淑军房耀维刘姝焦豫良李瑛曹茜
Owner HUAIHAI INST OF TECH
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