29 results about "Cellobiose" patented technology

The invention discloses a cellobiose 2-epimerase mutant as well as a gene, an expression vector, recombinant bacteria, a preparation method and application thereof. The mutant is obtained by performing the following point mutation on an amino acid sequence as shown in SEQ ID No.1: S12A, S24A, G131A, G176A, G224A, K46R, K69R, K72R, K91R, K103R, K201R, K219R, K223R, K241R, K263R, K266R, K273R, K284R, K325R, K335R, K349R and K387R. The invention further discloses a preparation method of the mutant. The invention further discloses a gene segment, a plasmid and a recombinant bacterium for recombinant expression of the mutant, and the gene segment, the plasmid and the recombinant bacterium are used for recombinant expression preparation of the mutant. The mutant can be used for continuously and stably catalyzing and preparing lactulose at high temperature, is relatively good in thermal stability and has a relatively good industrial application prospect.

In the present application, by knocking out the BG gene in Trichoderma reesei PX3 (a strain of cellulase transcription activator that can be activated continuously), the BG activity can be reduced under the condition of not reducing or even increasing the filter paper enzyme activity of the enzyme system, and a low BG enzyme activity and high filter paper enzyme activity strain is constructed. This strategy can avoid the negative impact on cellulase production after BG knockout, and the obtained strain can be used for efficient production of cellobiose or as a chassis strain for producing cellulase.

The invention discloses a flexible glass cutting fluid and a preparation method thereof, and the preparation method comprises the following steps: S1, adding cellobiose into DMF, then adding chloroacetic anhydride and pyridine, and carrying out a stirring reaction to obtain an intermediate; s2, adding the intermediate into DMF (Dimethyl Formamide), and then carrying out constant-temperature reaction on glycerol monocaprylate, 2-(2-hydroxyphenyl) benzothiazole and triethylamine to obtain a modifier; s3, uniformly mixing a modifier, sodium dioctyl sulfosuccinate and a polyoxyethylene polyoxypropylene block copolymer, so as to obtain a composite surfactant; s4, adding the composite surfactant, the propylene glycol, the defoaming agent, the sterilizing agent and the triethanolamine into the water, and uniformly stirring to obtain the cleaning agent. According to the prepared cutting fluid, the protection effect on a flexible glass base material and a surface functional layer of the flexible glass base material is remarkably improved while the excellent wettability is kept, microcrack generation and surface damage in the cutting process are reduced, and the cutting fluid is particularly suitable for precise glass processing of flexible display devices.

The present application relates to a kind of insecticidal compositions containing flonicamid and celangulin, wherein the weight percentage of effective component flonicamid and celangulin is 1-80:10-0.1, which can be further preferred as 10-60:5-0.1.The total mass of two effective components flonicamid and celangulin accounts for 1%-90% of the mass of the entire preparation, which can be further preferred as 10-60%, and the rest is the common adjuvant or filler in pesticides.The insecticidal composition of the present application can be prepared into suspension concentrate, water dispersible granules, water emulsion, microemulsion, emulsifiable concentrate and wettable powder and other dosage forms.The present application carries out binary compounding of flonicamid and celangulin, has obvious synergistic effect, and the quick-acting and long-acting properties of pesticide are good, and reduces production cost and use cost, and is especially suitable for the prevention and treatment of thrips.

The application provides a kind of complex hydrolytic enzyme and its preparation method, it is related to enzyme engineering technical field.The preparation method includes: aspergillus niger is inoculated in culture medium to carry out fermentation, and the fermentation liquor containing beta-glucosidase is collected;The fermentation liquor is subjected to solid-liquid separation to obtain beta-glucosidase preparation;Add alpha-L-arabinofuranosidase and cellobiohydrolase to obtain the complex hydrolytic enzyme.This technology uses specific content of sodium carboxymethyl cellulose and wheat bran to synergistically ferment, the activity and specificity of the main enzyme beta-glucosidase are greatly improved by nutritional induction and conformation simulation;Subsequently, alpha-L-arabinofuranosidase and cellobiohydrolase are compounded, the single enzyme limitation is broken and the feedback inhibition is removed, and the overall catalytic efficiency and conversion rate are significantly improved.

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition and a preparation method and application thereof, active ingredients of the traditional Chinese medicine composition including a fermentation product prepared by subjecting traditional Chinese medicine raw materials to anaerobic solid-state fermentation in combination with Lactobacillus helveticus and enzyme preparation; the traditional Chinese medicine raw materials include ginseng, poria cocos, Chinese yam, white lentil, lotus seed, yiyi seed, amomum villosum, platycodon grandiflorum, licorice and dried tangerine or orange peel; and the enzyme preparation includes cellulase and / or cellobiase. The application effectively promotes dissolution of effective ingredients of Shenlingbaizhu powder, significantly enriches antioxidant active ingredients in the fermentation product, relieves inhibition of oxidative stress on immune cells, and greatly improves survival rate and phagocytic efficacy of macrophages, so that the application shows a significant antibacterial pneumonia effect and has an excellent application prospect.

Solutions and methods are disclosed for the extraction and quantification of cellulose derived from cellulosic biomass samples. Improved extraction yields and selectivities are provided through the use of an extraction solvent mixture comprising an aprotic solvent such as DMSO, a quaternary ammonium salt such as TBAF, and an quaternary ammonium base such as TBAOH. The extracted cellulose can be optionally precipitated using disclosed precipitation solutions to further improve cellulose purity. Extracted cellulose can be measured by hydrolyzing the cellulose to glucose or cellobiose, or by using disclosed spectrophotometric assays of cellulose-salt complexes.

The following methods enhance the sweetness of sugars: A sugar or a sugar-containing sweetening composition is blended with at least one selected from the group consisting of oligosaccharides and megalosaccharides, in which two or more fructose molecules are bonded to glucose, in a proportion that does not exhibit sweetness. The sugar is at least one selected from the group consisting of sucrose, maltose, glucose, fructose, trehalose, and cellobiose.

The present invention relates to the use of cellobiofructose (CBF) as a dietary fiber and / or prebiotic component in food products, food product additives, nutraceuticals and / or beverages or for increasing the growth of probiotic microorganisms. The invention further relates to CBF and compositions containing it for use in the prevention and / or treatment of obesity, constipation, high cholesterol, diabetes, hypertension, and / or heart disease. The invention further encompasses a method for the (recombinant) production of cellobiofructose (CBF), as described herein, using a polypeptide having levansucrase activity which comprises or consists of a polypeptide having at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 1.

A process to hydrolyze cellulose into cellobiose comprising the following steps: - providing a reaction vessel; - providing a Cellulomonas uda (ATCC 491) inoculum; - exposing said Cellulomonas uda (ATCC 491) bacterium to a source of cellulose having a kappa number of less thanin an aqueous medium of pH of about 8 at a temperature ranging from 30 °C to 35 °C for a period of time ranging from 14 to 42 days; - exposing the cellobiose to a bacterium or fungi or yeast, or combination which converts cellobiose to glucose or ethanol.

The invention relates to a preparation method of an icaritin double-cellobiose derivative in the field of medicine and chemical synthesis, which comprises the following steps: icaritin I and bromoacetyl cellobiose II are put into a solvent, and are catalyzed by an inorganic base and a phase transfer catalyst to react to obtain an icaritin double-cellobiose derivative III, and the reaction route is shown in the specification. When the icaritin double-cellobiose derivative III is prepared, icaritin and bromoacetyl cellobiose which are easy to obtain are used as starting raw materials, the price is low, the chemical catalysis process is simple, the reaction condition is mild, the reaction yield is high (36%-76%), and industrial production is easy.

This method for producing hydrogen gas comprises a step for obtaining hydrogen gas by irradiating a mixture containing a substrate containing at least one substance selected from the group consisting of chitin, cellulose, hemicellulose, lignin, glucose, cellobiose, starch, and alcohol and a catalyst containing a base metal component with light, the mixture containing a substrate containing at least one substance selected from the group consisting of chitin, cellulose, hemicellulose, lignin, glucose, cellobiose, starch, and alcohol.

This invention belongs to the field of microbial technology, and discloses a highly efficient anaerobic fungal strain for degrading lignocellulose in the rumen of Dulong cattle, its cultivation method, and its applications. The strain is *Neisseria gonorrhoeae*. Joblinomyces apicalis DLM2, deposited at the China Center for Type Culture Collection (CCTCC) under accession number CCTCC NO: M 20252927, was isolated from the rumen of Dulong cattle and can be stably cultured under strictly anaerobic conditions. This strain possesses a single-centered cell line and filamentous rhizoids, enabling it to efficiently degrade lignocellulose through a synergistic effect of physical colonization and enzymatic degradation. It exhibits broad adaptability to natural straws such as rice straw and bamboo branches, as well as purified substrates such as microcrystalline cellulose and cellobiose. Its enzyme production exhibits substrate-inducible properties, and fermentation produces various metabolites including formic acid, acetic acid, and ethanol, demonstrating high lignocellulose degradation efficiency. This invention relates to an inoculum containing this strain and its application in lignocellulose degradation, roughage fermentation, agricultural straw resource utilization, and biomass conversion, providing new strain resources and technical support for the efficient degradation and resource utilization of lignocellulose.

This invention discloses a reagent kit and method for the joint detection of glucose, cellobiose, and xylose in cellulose hydrolysate, belonging to the field of biomass conversion and analytical detection technology. The method includes the following steps: setting up three types of detection channels: a glucose oxidase detection channel that specifically responds to the glucose component; a β-glucosidase-glucose oxidase combined detection channel that responds to the total signal of glucose and cellobiose; and a pyranose oxidase detection channel that simultaneously responds to the combined signals of glucose, cellobiose, and xylose. Enzymatic colorimetric reactions are performed on the cellulose hydrolysate sample and the multi-component gradient standard solution in the three types of detection channels, respectively. A ternary linear calibration model is constructed based on the absorbance data of the three types of channels of the multi-component gradient standard solution. This method provides a rapid, low-cost, and highly specific detection method for glucose, cellobiose, and xylose in cellulose biomass hydrolysate, and has practical application value.

Provided are a southern corn leaf blight-resistant Thph2 protein from Trichoderma harzianum, a ZmGLP1-17 protein from Zea mays L., and a method for preparing transgenic Zea mays L. Further provided are a cellobiohydrolase protein Thph2 from Trichoderma harzianum T30, a coding gene for the cellobiohydrolase protein, and use of the cellobiohydrolase protein in inducing a response of a GLP ZmGLP1-17 from Zea mays L. against southern corn leaf blight. Trichoderma harzianum strain OEthph2 overexpressing a Thph2 gene is constructed by a transgenic technique. OEthph2 may induce the expression of the GLP ZmGLP1-17 in roots of Zea mays L. and enhance the resistance of leaves of Zea mays L. to the infection of Cochliobolus heterostrophus. ZmGLP1-17 transgenic Zea mays L. material is constructed. The OEthph2 engineered strain may be combined with the ZmGLP1-17 transgenic Zea mays L. material to synergistically improve a control effect for southern corn leaf blight.

The application discloses a method for preparing high-value cellobiose from a medicine and food homologous Radix Millettiae and application of the high-value cellobiose. The method comprises the following steps: extracting cellulose from Radix Millettiae; optimizing a beta-glucanase enzymolysis process by using a response surface method; after the enzymolysis liquid is subjected to selective removal of glucose through yeast fermentation, the high-value cellobiose product is obtained through acetone precipitation and freeze-drying. The process condition is mild and environment-friendly, high-value utilization of agricultural waste is realized, the obtained cellobiose product has high purity, and an economic and effective approach is provided for large-scale production of cellobiose. Animal experiments prove that the cellobiose product has a significant relieving effect on colonitis induced by dextran sulfate sodium in mice, can effectively improve disease symptoms, reduce oxidative stress, repair intestinal barrier and regulate inflammatory response, and has a good application prospect in preparation of functional food or medicine for relieving colonitis and high-value utilization of Radix Millettiae.

According to the method, firstly, high-proportion bamboo powder is used as a carbon source, and by means of comprehensive regulation and control of bamboo flavones, cellobiose synthesized through fermentation and the like in the bamboo powder, cellulase producing genes and the like of bamboo-decomposing bacteria are induced and activated, so that the bamboo-decomposing bacteria are obtained; meanwhile, the cultivation environment, self-space hybridization, fusion, seed liquid, an enzymolysis agent, a permeation stabilizer, strain enrichment and the like of the bamboo degradation strains are regulated and controlled, and combined bacteria such as bacillus subtilis, aspergillus oryzae, lactic acid bacteria, saccharomycetes, rhizopus and the like are prepared; then the bamboo decomposition combined bacteria synergistically and interactively efficiently degrade nitrogen source bamboo fibers in directional fermentation under substrate optimization and multi-dimensional comprehensive regulation and control conditions, the nitrogen source bamboo fibers are converted and synthesized into high-quality mycoprotein, digestible bamboo protein, soluble sugar and other nutrient substances, the repression effect in the bamboo decomposition fermentation process is eliminated, and the bamboo decomposition effect is improved. The problems of low fermentation strain activity, low adaptability, low efficiency, poor synergy, biological safety, stability and the like are solved.

The invention discloses a radix sophorae flavescentis composite traditional Chinese medicine preparation for reducing soft-shell eggs and sand-preserved eggs of laying hens as well as a preparation method and application thereof, and relates to the technical field of traditional Chinese medicine preparations. The radix sophorae flavescentis composite traditional Chinese medicine preparation is prepared by mixing radix sophorae flavescentis, radix astragali, herba epimedii, herba leonuri, pine needles, chestnut shells and licorice roots to serve as a traditional Chinese medicine composition, adopting lactobacillus plantarum, lactobacillus cellobiosus and bacillus lentus as fermentation bacteria liquid, inoculating the traditional Chinese medicine composition with the composite fermentation bacteria liquid, and conducting fermentation, drying and smashing. Through cooperation of the traditional Chinese medicine composition and the zymophyte liquid, the production of soft-shell eggs and sand-preserved eggs can be reduced. In addition, the lactobacillus plantarum, the lactobacillus cellobiosus and the bacillus lentus are combined to serve as the zymophyte liquid, and the zymophyte liquid has a synergistic effect on reducing soft-shell eggs and sand-preserved eggs laid by the laying hens.

本发明公开了一种低温条件下兼具玉米与大豆秸秆降解能力的复合菌剂及其制备方法应用。所述的复合菌剂由保藏编号为CGMCC No.42446的木霉菌（Trichoderma sp.）FS5以及保藏编号为CGMCC No.37567的铜绿假单胞菌（Pseudomonas chlororaphis subsp.）BNT4组成。其中木霉菌FS5能将纤维素分解成较短的纤维寡糖或纤维二糖，铜绿假单胞菌BNT4则可以利用这些中间产物作为碳源，从而避免了这些产物积累对木霉菌FS5产生反馈抑制，间接促进了整个降解过程的效率，从而形成一套高效率的秸秆降解“流水线”。本发明提供的复合菌剂为东北地区主粮作物秸秆的绿色有效处理提供了新的解决方案，对于促进土壤固碳固氮，提高土地肥力及实现农业可持续发展具有重要意义。

The present invention relates to the use of a mono-ester glycolipid or a mixture of mono-ester glycolipids in an automatic dishwasher detergents composition. The mono-ester glycolipid or mixture of mono-ester glycolipids comprises a carbohydrate moiety is selected from the group consisting of maltose, cellobiose, trehalose, isomaltulose, lactulose, isomaltose, and mixtures thereof.

The invention discloses kluyveromyces marxianus and application of the kluyveromyces marxianus in production of single-cell protein. The kluyveromyces marxianus disclosed by the invention is a wild strain separated from soil, and has the characteristics of wide growth temperature range and high temperature resistance; monosaccharide and disaccharide obtained by degrading lignocelluloses such as glucose, xylose and cellobiose and tricarboxylic acid cycle metabolites of microbial cells such as succinic acid, citric acid, malic acid and fumaric acid can be rapidly metabolized, and microbial protein can be accumulated. The invention provides an excellent strain capable of being used for producing single-cell protein, and the strain has wide application value in the field of microbial protein.

The application discloses an engineered Trichoderma reesei strain with high yield of beta-glucosidase, and a construction method and application thereof. In view of the problems of low beta-glucosidase activity of a natural cellulase system of Trichoderma reesei in the prior art and easy accumulation of cellobiose to cause feedback inhibition, the application takes Trichoderma reesei RUT-C30 as a starting strain, and through three-step progressive operation, firstly, a beta-glucosidase gene expression cassette derived from Aspergillus niger is integrated into a URA5 gene locus; then, an expression cassette containing a URA5 back complement unit and a BGL gene is constructed and integrated into an ACE1 gene site; finally, a transcription activator XYR1 expression cassette is integrated into a PEP1 site. Through the above iterative modification, an engineered strain with stable genetic traits, capable of simultaneously high-level secretion of beta-glucosidase and filter paper enzyme is obtained. It is verified through experiments that the beta-glucosidase activity of the engineered strain can be up to 80.6 IU / mL, which is 268.3 times that of the starting strain.

Provided are methods for transducing a molecule(s) of interest into a cell, comprising a step of contacting the cell with the molecule(s) of interest and a solution for transduction, the solution for transduction containing: at least one of the following (A1) to (A5); and (B) a salt: (A1) a compound represented by formula (I) excluding a predetermined compound, or a salt thereof; (A2) a compound represented by formula (II), or a salt thereof; (A3) a nucleic-acid base or the like, or a salt thereof; (A4) a compound represented by formula (III), or a salt thereof, excluding malic acid; and (A5) at least one selected from the group consisting of creatinine, hydroxyproline, 1,3-butanediol, trientine, D-cellobiose, 1,3-dimethylurea, pantolactone and trimethadione, or a salt thereof.

The present application relates to the technical field of food / functional food or health food, in particular to a composition and its preparation method and application.The composition comprises the following components: coffee raw materials, herbal extract; the preparation raw materials of the herbal extract comprise green tea, bitter gourd, licorice, cassia seed and lotus leaf; the preparation method of the herbal extract comprises the following steps: (1) mixing the preparation raw materials according to the proportion, extracting by water to obtain an extract; (2) the extract is subjected to enzymatic hydrolysis treatment by cellulase, pectinase, cellobiase and tannase, and solid-liquid separation, and the liquid phase is the herbal extract.The composition of the present application can effectively inhibit fat absorption, has good weight loss effect, and can reduce body weight, BMI index, waist circumference, hip circumference, abdominal fat thickness and adverse eating behavior.

The invention relates to the technical field of biology, in particular to a 3 'UTR element for enhancing upstream gene expression of zymomonas mobilis and application of the 3' UTR element. The 3 'UTR element provided by the invention can stably improve the expression level of upstream genes. When the 3 'UTR element is applied to a target gene for coding beta-glucosidase, the beta-glucosidase activity of zymomonas mobilis recombinant engineering bacteria can be remarkably improved, so that the requirement of the zymomonas mobilis recombinant engineering bacteria on efficient utilization of cellobiose substrates is met, and the yield of ethanol is finally improved. The invention provides a new key tool for realizing fine regulation and control and metabolic flux optimization of multiple genes in a metabolic pathway, and further effectively improves the application potential of the Zymomonas mobilis as a microbial cell factory.

The invention discloses a multienzyme system for efficiently synthesizing beta-1, 3-glucan oligosaccharide through a one-pot method and application, and belongs to the technical field of gene engineering and modern enzyme engineering. The key point of the method is that various high-activity G1P donor generating enzymes and beta-1, 3-gluco-oligosaccharide phosphorylase are systematically screened and optimally combined, and the beta-1, 3-gluco-oligosaccharide with specific polymerization degree distribution can be directly synthesized in a single reactor through a'one-pot method 'by taking cheap and easily available cane sugar, cellobiose or maltodextrin as raw materials. According to the invention, the molar conversion rate of sucrose can reach 98.24%, and beta-1, 3-glucan oligosaccharide in an enzyme reaction product in the 72h can reach DP18, so that the dependence on expensive nucleotide activated sugar is thoroughly eliminated. By adjusting reaction parameters and directionally enriching a product with a target polymerization degree, a green, economical, efficient and large-scale brand-new production path is provided for the functional sugar.

The application discloses a beta-glucosidase mutant and application thereof in degrading cellulose. The application obtains the beta-glucosidase mutant with improved thermal stability and / or enzyme activity by mutating specific amino acid sites of wild-type beta-glucosidase Ks5A7 and mutant Ks5A7-4R1 based on the wild-type beta-glucosidase Ks5A7. Compared with the parent, the obtained mutant can more efficiently catalyze hydrolysis of cellobiose at a higher temperature, which can accelerate the speed of degrading cellulose into fermentable monosaccharide, and further accelerate the process of producing biofuel and bio-based chemicals by using cellulose. In addition, the application takes the recombinant bacteria expressing the mutant M10 as an example to carry out high-cell-density fermentation, and finds that the mutant M10 has efficient expression characteristics and good industrial application prospect. The application is beneficial to biomass conversion of cellulose and industrialized preparation of biofuel and bio-based chemicals.

The present invention relates to cellobiohydrolase variants and carbohydrate binding module variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

The invention discloses a glucoside hydrolase capable of degrading cellobiose and maltotriose. The glucoside hydrolase is characterized in that the amino acid sequence of the glucoside hydrolase is SEQ ID NO. 1. The strain has relatively high activity on cellobiose and maltotriose, the enzyme activities are respectively 0.1 U and 0.102 U, and the specific activities are respectively 80.4 U / mg and 82.0 U / mg. The invention aims to enrich the existing carbohydrate enzyme gene bank and develop a novel efficient enzyme preparation. According to the present invention, the novel efficient carbohydrate enzyme is explored, and has efficient degradation ability on cellobiose and maltotriose, such that the reference is provided for the exploration of the novel enteric microorganism encoded CAZYmes. CAZymes generated by animal intestinal microorganisms are beneficial to decomposition of complex carbohydrates, the carbohydrates are difficult to decompose in a digestive system of a host, and the carbohydrate active enzyme obtained through excavation is expected to improve the digestion efficiency. By studying CAZymes in intestinal microorganisms, a novel enzyme preparation is expected to be developed and used in food industry, feed industry or as a therapeutic agent.