114 results about "Cellobiose" patented technology

The invention discloses a method for preparing cellobiose and comprehensively utilizing a by-product of cellobiose. The method is characterized by specifically comprising the following steps: (1) dilution, (2) enzymolysis, (3) flocculation and carbon explosion, (4) decoloration, (5) continuous ion exchange, (6) evaporation and concentration, (7) simulated moving bed chromatographic separation, (8) concentration, (9) finished product preparation and (10) raffinate utilization. The method effectively solves the problems of low preparation purity and high production cost of cellobiose.

The invention discloses a cellobiose 2-epimerase mutant as well as a gene, an expression vector, recombinant bacteria, a preparation method and application thereof. The mutant is obtained by performing the following point mutation on an amino acid sequence as shown in SEQ ID No.1: S12A, S24A, G131A, G176A, G224A, K46R, K69R, K72R, K91R, K103R, K201R, K219R, K223R, K241R, K263R, K266R, K273R, K284R, K325R, K335R, K349R and K387R. The invention further discloses a preparation method of the mutant. The invention further discloses a gene segment, a plasmid and a recombinant bacterium for recombinant expression of the mutant, and the gene segment, the plasmid and the recombinant bacterium are used for recombinant expression preparation of the mutant. The mutant can be used for continuously and stably catalyzing and preparing lactulose at high temperature, is relatively good in thermal stability and has a relatively good industrial application prospect.

The invention discloses enterococcus faecalis YL-EF32 and application thereof, and belongs to the technical field of microorganisms. The collection number of the enterococcus faecalis YL-EF32 provided by the invention is CCTCC (China Center For Type Culture Collection) NO: M 20242241. The strain can effectively inhibit the proliferation of chicken-derived clostridium perfringens, has better tolerance to cholate, artificial gastric juice and phenol, and can metabolize dietary fibers such as pectin, glucan, cellobiose and the like. Enterococcus faecalis YL-EF32 is added into daily ration of broiler chickens, necrotic enteritis induced by coccidiosis and clostridium perfringens can be remarkably improved by means of enhancing intestinal barriers, balancing intestinal flora stability, relieving inflammatory response and the like, and the enterococcus faecalis YL-EF32 can be used for resisting clostridium perfringens infection in livestock and poultry breeding.

The invention discloses heat-resistant alpha-glucan phosphorylase and application thereof in synthesis of amylose with different molecular weights, and belongs to the field of genetic engineering and modern enzyme technology engineering. The alpha-glucan phosphorylase derived from the Dictyoglomus. Sp.NZ13RE01, provided by the invention, has a relatively high optimum temperature and excellent thermal stability, and can be used for preparing the alpha-glucan phosphorylase from the Dictyoglomus. Sp.NZ13RE01. When the alpha-glucan phosphorylase is used for synthesizing amylose, the alpha-glucan phosphorylase from the source can be used for synthesizing amylose with different molecular weights under the condition of cellobiose / G4 primers in different proportions, and under the condition that the proportion of cellobiose / maltotetraose primers is 1: 4, the excellent conversion rate is 34.52%, the yield is 17.26 g / L, and the yield is 17.26 g / L. The average molecular weight is 5040 + / -26.75 g / moL, so that the method is suitable for producing amylose in a small molecular weight range, and therefore, the method has a good application prospect in an artificially synthesized starch system.

In the present application, by knocking out the BG gene in Trichoderma reesei PX3 (a strain of cellulase transcription activator that can be activated continuously), the BG activity can be reduced under the condition of not reducing or even increasing the filter paper enzyme activity of the enzyme system, and a low BG enzyme activity and high filter paper enzyme activity strain is constructed. This strategy can avoid the negative impact on cellulase production after BG knockout, and the obtained strain can be used for efficient production of cellobiose or as a chassis strain for producing cellulase.

The invention relates to a pig immunity-enhancing feed containing natural plant extracts, and belongs to the technical field of feeds. On the basis of basic components, a plantain herb and selfheal composite extract, a purslane and sweet potato vine and leaf composite extract and a folium cortex eucommiae extract are also added into the feed, so that the immunity-enhancing feed is prepared. The plantain herb and selfheal composite extract is obtained by sequentially fermenting a plantain herb whole plant and a selfheal whole plant through lactobacillus cellobiosus and pediococcus acidilactici and then sequentially carrying out step-by-step enzymolysis through trypsin and nattokinase; the herba portulacae and sweet potato vine compound extract is obtained by sequentially fermenting a whole plant of herba portulacae and leaves of sweet potato vines with lactobacillus cellobiosus and bacteroides ruminalis and then sequentially carrying out step-by-step enzymolysis with snail protease and trypsin; the folium cortex eucommiae extract is obtained by sequentially carrying out step-by-step enzymolysis on folium cortex eucommiae through hemicellulase, bromelain and nattokinase. The extract prepared by the invention contains immunological enhancement effective components, and can enhance the immunity of pigs, effectively reduce diarrhea and reduce the risk of illness when being matched with basal feed for use.

The invention provides a colonic microbiota responsive polymer characterized in that it is prepared by the polymerization of a response monomer and a linking monomer, wherein the response monomer is a cellobiose with a β-(1,4)-glucoside bond or / and a lactose with a β-galactoside bond; the linking monomer is a monomer that reacts with the hydroxyl group of the response monomer to form an ester or ether directly or by a crosslinking agent. The invention also provides a method for preparation of a colonic microbiota responsive polymer, a degradable film based on the polymer and a preparation method thereof. By means of the characteristics that β-(1,4)-glucoside bond provided by cellobiose or / and β-galactoside bond provided by lactose monomer unit in the polymer can specifically respond to the high abundance of microbiotas (β-glucosidase or / and β-galactosidase) in the colon to specifically degrade in the colon, a colon-targeted delivery system for diagnosis and treatment of colon diseases is constructed.

The invention discloses a flexible glass cutting fluid and a preparation method thereof, and the preparation method comprises the following steps: S1, adding cellobiose into DMF, then adding chloroacetic anhydride and pyridine, and carrying out a stirring reaction to obtain an intermediate; s2, adding the intermediate into DMF (Dimethyl Formamide), and then carrying out constant-temperature reaction on glycerol monocaprylate, 2-(2-hydroxyphenyl) benzothiazole and triethylamine to obtain a modifier; s3, uniformly mixing a modifier, sodium dioctyl sulfosuccinate and a polyoxyethylene polyoxypropylene block copolymer, so as to obtain a composite surfactant; s4, adding the composite surfactant, the propylene glycol, the defoaming agent, the sterilizing agent and the triethanolamine into the water, and uniformly stirring to obtain the cleaning agent. According to the prepared cutting fluid, the protection effect on a flexible glass base material and a surface functional layer of the flexible glass base material is remarkably improved while the excellent wettability is kept, microcrack generation and surface damage in the cutting process are reduced, and the cutting fluid is particularly suitable for precise glass processing of flexible display devices.

The present application relates to a kind of insecticidal compositions containing flonicamid and celangulin, wherein the weight percentage of effective component flonicamid and celangulin is 1-80:10-0.1, which can be further preferred as 10-60:5-0.1.The total mass of two effective components flonicamid and celangulin accounts for 1%-90% of the mass of the entire preparation, which can be further preferred as 10-60%, and the rest is the common adjuvant or filler in pesticides.The insecticidal composition of the present application can be prepared into suspension concentrate, water dispersible granules, water emulsion, microemulsion, emulsifiable concentrate and wettable powder and other dosage forms.The present application carries out binary compounding of flonicamid and celangulin, has obvious synergistic effect, and the quick-acting and long-acting properties of pesticide are good, and reduces production cost and use cost, and is especially suitable for the prevention and treatment of thrips.

The present invention relates to a pig immune-enhancing feed containing natural plant extracts, belonging to the technical field of feeds. On the basis of the basic components, the feed of the present invention also adds a composite extract of plantain and selfheal, a composite extract of purslane and sweet potato vine leaves, and eucommia leaf extract. The composite extract of plantain and selfheal is obtained by successively fermenting the whole plant of plantain and the whole plant of selfheal with Lactobacillus cellobiosus and Pediococcus acidilactici, and then successively subjecting them to enzymatic hydrolysis with trypsin and nattokinase step by step; the composite extract of purslane and sweet potato vine leaves is obtained by successively fermenting the whole plant of purslane and sweet potato vine leaves with Lactobacillus cellobiosus and Bacteroides ruminicola, and then successively subjecting them to enzymatic hydrolysis with snail protease and trypsin step by step; the eucommia leaf extract is obtained by successively subjecting eucommia leaves to enzymatic hydrolysis with hemicellulase, bromelain, and nattokinase step by step. The extract contains immune-enhancing active ingredients, and when used in combination with the basic feed, it can enhance the immunity of pigs, effectively reduce diarrhea, and reduce the risk of disease.

The application provides a kind of complex hydrolytic enzyme and its preparation method, it is related to enzyme engineering technical field.The preparation method includes: aspergillus niger is inoculated in culture medium to carry out fermentation, and the fermentation liquor containing beta-glucosidase is collected;The fermentation liquor is subjected to solid-liquid separation to obtain beta-glucosidase preparation;Add alpha-L-arabinofuranosidase and cellobiohydrolase to obtain the complex hydrolytic enzyme.This technology uses specific content of sodium carboxymethyl cellulose and wheat bran to synergistically ferment, the activity and specificity of the main enzyme beta-glucosidase are greatly improved by nutritional induction and conformation simulation;Subsequently, alpha-L-arabinofuranosidase and cellobiohydrolase are compounded, the single enzyme limitation is broken and the feedback inhibition is removed, and the overall catalytic efficiency and conversion rate are significantly improved.

The invention discloses foal bacillus capable of efficiently utilizing cellobiose to produce bacterial cellulose and a construction method and application thereof, the foal bacillus capable of efficiently utilizing cellobiose to produce bacterial cellulose (Komagaeibacter xylinus) GX071 (CGMCC No.26247) or the foal bacillus capable of efficiently utilizing cellobiose to produce bacterial cellulose (Komagaeibacter xylinus) GX073 (CGMCC No.26248) is fermented, the utilization rate of GX071 cellobiose is increased by 19%, the yield of bacterial cellulose is increased by 24%, and the yield of bacterial cellulose is increased by 24%. The utilization rate of cellobiose of GX073 is increased by 31%, and the yield of bacterial cellulose is increased by 38%. Lignocellulose resources such as forestry and agricultural residues are effectively utilized, green biological manufacturing is achieved, the production cost is effectively reduced, and the requirement for large-scale industrial production of bacterial cellulose is met.

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition and a preparation method and application thereof, active ingredients of the traditional Chinese medicine composition including a fermentation product prepared by subjecting traditional Chinese medicine raw materials to anaerobic solid-state fermentation in combination with Lactobacillus helveticus and enzyme preparation; the traditional Chinese medicine raw materials include ginseng, poria cocos, Chinese yam, white lentil, lotus seed, yiyi seed, amomum villosum, platycodon grandiflorum, licorice and dried tangerine or orange peel; and the enzyme preparation includes cellulase and / or cellobiase. The application effectively promotes dissolution of effective ingredients of Shenlingbaizhu powder, significantly enriches antioxidant active ingredients in the fermentation product, relieves inhibition of oxidative stress on immune cells, and greatly improves survival rate and phagocytic efficacy of macrophages, so that the application shows a significant antibacterial pneumonia effect and has an excellent application prospect.

To provide alternative and / or improved sweetness modifying composition.SOLUTION: There is provided a sweetness modifying composition comprising: one or more high-intensity sweetener(s) selected from steviol glycosides and / or mogrosides; and one or more low-intensity sweetener(s) selected from cellobiose, psicose, cyclamate and / or 11-O-mogroside V, wherein the sweetness modifying composition increases the sweetness of a sweetened composition by more than the sweetness of the sweetness modifying composition alone; and / or wherein the ratio of the one or more high-intensity sweetener(s) to the one or more low-potency sweetener(s) ranges from about 2:1 to about 12:1.SELECTED DRAWING: None

The present invention discloses a salt-tolerant β-glucosidase and application, and relates to the field of biotechnology. The amino acid sequence of the β-glucosidase is shown in SEQ ID NO: 1. The present invention clones a novel β-glucosidase functional gene b0-bg40 belonging to the GH1 family from Karamay saline-alkali land using metagenomic technology, and realizes heterologous expression in Escherichia coli. The enzymatic properties of b0-bg40 are then characterized, and it is found that b0-bg40 is an alkali-resistant, thermally stable, and pH-wide β-glucosidase. In addition, b0-bg40 is tolerant to glucose and has a high affinity for cellobiose. These findings indicate that it has potential utilization value in the degradation of cellulose biomass and production in strong alkaline environments.

The invention discloses a disaccharide monosubstituted derivative of Lamellarin D and N. The structure of the disaccharide monosubstituted derivative is as follows: R1 and R2 are respectively and independently selected from one of H, methyl, benzyl, acetyl and disaccharide, R3 is selected from H, benzyl or disaccharide, R4 is selected from H, methylsulfonyl or disaccharide, and at least one of R1-R4 is disaccharide; the disaccharide group is one of cellobiose group, acetylated cellobiose group, lactosyl group, acetylated lactosyl group, maltose group and acetylated maltose group. According to the present invention, at least one disaccharide group is introduced into R1-R4 to obtain the disaccharide mono-substituted derivative of Lamellarin D and N, the proliferation inhibition activity of tumor cells is improved with the compound and the monosaccharide substituted derivative, and the inhibition effect on non-solid tumor cell line Jurkat is significantly improved by about 5 times;

The invention provides a multienzyme system for preparing cellobiose as well as a preparation method and application of cellobiose, and belongs to the technical field of bioengineering. The multi-enzyme system disclosed by the invention is prepared from endoglucanase, cellobiose hydrolase and lysed polysaccharide monooxygenase, wherein the endoglucanase is EG5C-1m, the cellobiose hydrolase is TeCel7A T393K, and the lysed polysaccharide monooxygenase is LsAA9A. According to the method disclosed by the invention, a multi-enzyme system for accurately degrading cellulose into cellobiose is constructed by screening high-efficiency endoglucanase, cellobiose hydrolase and lysed polysaccharide monooxygenase. On the basis, the optimal reaction condition of a multi-enzyme system is determined, a method is provided for efficient generation of cellobiose from cellulose, and a basis is provided for subsequent biomass conversion of high-value products.

The present application discloses a cellobiohydrolase mutant which can provide degradation function in cellulose degradation, and the cellobiohydrolase mutant is a protein of (a) or (b) as follows: aspartic acid at position 259 of the amino acid sequence shown in SEQ ID NO:1 is substituted into other amino acid, the other amino acid is valine or tryptophan, (b) a protein derived from (a) with cellobiohydrolase activity by substituting, deleting or adding one or more amino acids in the amino acid sequence of (a). The present application also discloses the application of the cellobiohydrolase mutant or composition in cellulose hydrolysis. The cellobiohydrolase mutant of the present application has the activity of hydrolyzing cellulose, and the mutant with improved activity is obtained, and the hydrolysis effect on cellulose is improved.

Solutions and methods are disclosed for the extraction and quantification of cellulose derived from cellulosic biomass samples. Improved extraction yields and selectivities are provided through the use of an extraction solvent mixture comprising an aprotic solvent such as DMSO, a quaternary ammonium salt such as TBAF, and an quaternary ammonium base such as TBAOH. The extracted cellulose can be optionally precipitated using disclosed precipitation solutions to further improve cellulose purity. Extracted cellulose can be measured by hydrolyzing the cellulose to glucose or cellobiose, or by using disclosed spectrophotometric assays of cellulose-salt complexes.

The application relates to a method for preparing natural gas by taking biomass and polyhydroxy compounds as raw materials. The method comprises the following steps: mixing the raw materials with water and a catalyst, and carrying out a reduction reaction in a reducing atmosphere to obtain a product containing natural gas; the raw materials are selected from biomass raw materials or / and polyhydroxy compounds; the biomass raw materials are selected from at least one of beech sawdust, poplar sawdust, pine sawdust, birch sawdust, wheat straw or corn straw; the polyhydroxy compounds are selected from at least one of cellulose, hemicellulose, cellobiose, xylose, guaiacol, sorbitol, isosorbide, glycerol, erythritol, xylitol or glucose; the catalyst comprises a carrier and an active component loaded on the surface of the carrier; the active component is selected from metal elements; the metal elements are selected from at least one of the single elements of ruthenium, rhodium, platinum, palladium or iridium. The method provides a path for converting biomass such as sawdust and polyhydroxy compounds to prepare natural gas, and has important industrial application prospects.

The present invention provides a method for introducing nucleic acids, proteins, or complexes thereof into cells with excellent efficiency, in particular, a method using a transduction solution. The method for transducing a molecule of interest into a cell of the present invention comprises the step of contacting the cell with the molecule of interest and a transduction solution, wherein the transduction solution comprises at least one of the following (A1) to (A5) and (B) a salt: (A1) a compound represented by the following general formula (I) or a salt thereof, but excluding a specified compound; (A2) a compound represented by the following general formula (II) or a salt thereof; (A3) a nucleic acid base or a salt thereof; (A4) a compound represented by the general formula (III) or a salt thereof, but excluding malic acid; (A5) at least one selected from the group consisting of creatinine, hydroxyproline, 1,3-butanediol, trientine, D-cellobiose, 1,3-dimethylurea, pantothenic acid, and trimethyldione, or a salt thereof. #imgabs0#

The invention discloses a method for preparing indole by catalyzing carbohydrates, which comprises the following steps: adding carbohydrates, water, aniline and a catalyst into a closed container, and carrying out high-temperature reaction in a hydrogen reducing atmosphere at the pressure range of 2-8MPa and the temperature range of 150-300 DEG C to obtain a mixed solution containing indole, the carbohydrate is any one or a combination of at least two of cellulose, cellobiose, glucose, fructose, starch and cane sugar. Therefore, by utilizing the characteristics that the catalyst exerts different catalytic activities in different components in the heterogeneous solvent, the heterogeneous solvent regulates and controls a reaction path, a high-activity silicon source in silicon dioxide and the like, mutual promotion or synergy between an intermediate product and the solvent, effects between double metals and effects between the metal and the silicon source are realized; therefore, the purpose that the catalyst catalyzes carbohydrates to generate indole in the solvent environment of water and aniline is achieved, and a new conversion way is provided for converting the carbohydrates into the indole.

The invention belongs to the technical field of bioengineering, and particularly relates to cellulase CelL7 as well as preparation, modification and application thereof. The amino acid sequence of the cellulase CelL7 is as shown in SEQ ID NO.2, and the cellulase CelL7 shows degradation capacity on sodium carboxymethyl cellulose, cellobiose and xylan and can promote the dispersion of methicillin-resistant staphylococcus aureus and pseudomonas aeruginosa biological membranes. The cellulase CelL7 and a CBM3 binding structural domain are fused, and modified cellulase CelL7-CBM3 is obtained; compared with cellulase CelL7, the modified cellulase CelL7-CBM3 has the advantages that the specific activity and the pH stability are improved. The method has a good application prospect.

The following methods enhance the sweetness of sugars: A sugar or a sugar-containing sweetening composition is blended with at least one selected from the group consisting of oligosaccharides and megalosaccharides, in which two or more fructose molecules are bonded to glucose, in a proportion that does not exhibit sweetness. The sugar is at least one selected from the group consisting of sucrose, maltose, glucose, fructose, trehalose, and cellobiose.

The invention belongs to the technical field of aquatic feed protein sources, and particularly relates to a method for increasing the protein content of DDGS (Distillers Dried Grains with Soluble). The method comprises the following steps: carrying out enzymolysis on corn DDGS by adopting hydrolase, and then carrying out enzyme inactivation, centrifugal separation and drying to obtain corn DDGS concentrated protein; the hydrolase is prepared from one or more of cellulase, xylanase, beta-glucolase, beta-glucosidase and cellobiase. According to the method, the protein content is increased through enzymolysis, and possible adverse effects of microorganisms used in a traditional method for increasing the DDGS protein content through microbial fermentation and metabolites of the microorganisms on the health of aquatic animals can be avoided. Meanwhile, the mycotoxin content of the DDGS can be reduced, and the vomitoxin content of the DDGS is further reduced from 2mg / kg to a degree which cannot be detected.

The present invention relates to the use of cellobiofructose (CBF) as a dietary fiber and / or prebiotic component in food products, food product additives, nutraceuticals and / or beverages or for increasing the growth of probiotic microorganisms. The invention further relates to CBF and compositions containing it for use in the prevention and / or treatment of obesity, constipation, high cholesterol, diabetes, hypertension, and / or heart disease. The invention further encompasses a method for the (recombinant) production of cellobiofructose (CBF), as described herein, using a polypeptide having levansucrase activity which comprises or consists of a polypeptide having at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 1.

A process to hydrolyze cellulose into cellobiose comprising the following steps: - providing a reaction vessel; - providing a Cellulomonas uda (ATCC 491) inoculum; - exposing said Cellulomonas uda (ATCC 491) bacterium to a source of cellulose having a kappa number of less thanin an aqueous medium of pH of about 8 at a temperature ranging from 30 °C to 35 °C for a period of time ranging from 14 to 42 days; - exposing the cellobiose to a bacterium or fungi or yeast, or combination which converts cellobiose to glucose or ethanol.

The invention relates to a preparation method of an icaritin double-cellobiose derivative in the field of medicine and chemical synthesis, which comprises the following steps: icaritin I and bromoacetyl cellobiose II are put into a solvent, and are catalyzed by an inorganic base and a phase transfer catalyst to react to obtain an icaritin double-cellobiose derivative III, and the reaction route is shown in the specification. When the icaritin double-cellobiose derivative III is prepared, icaritin and bromoacetyl cellobiose which are easy to obtain are used as starting raw materials, the price is low, the chemical catalysis process is simple, the reaction condition is mild, the reaction yield is high (36%-76%), and industrial production is easy.

The invention discloses a recombinant bacterium for expressing a BASP gene and a CGCBP gene and application of the recombinant bacterium in preparation of cellobiose. The present invention provides a recombinant microorganism having a BASP gene and a CGCBP gene. The invention also provides a method for preparing the recombinant microorganism for producing cellobiose, which comprises the following steps: introducing the BASP gene and the CGCBP gene into host bacteria to obtain the recombinant microorganism with the BASP gene and the CGCBP gene, namely the recombinant microorganism for producing cellobiose. The invention also provides a method for preparing cellobiose, which comprises the following step of: preparing cellobiose by taking cane sugar and glucose as raw materials and the recombinant microorganism as a whole-cell catalyst. The method can generate huge economic benefits for cellobiose production, and has great popularization and application values.

The invention discloses a surface treating agent for a positive electrode material of a lithium ion battery and application of the surface treating agent. The surface treating agent of the lithium ion battery positive electrode material disclosed by the invention comprises monosaccharide or oligosaccharide. The monosaccharide comprises one or more of aldose and ketose; and the oligosaccharide comprises one or more of lactose, fructo-oligosaccharide, maltose, cellobiose, trehalose, raffinose, pinotriose and gentiotriose. The surface treating agent forms a strong binding force with the transition metal on the surface of the positive electrode material, so that the solvation effect of the ester solvent in the electrolyte on the surface transition metal is weakened, and the surface transition metal is prevented from being dissolved in the electrolyte and deposited on the negative electrode side. Meanwhile, the catalytic decomposition process of the surface transition metal on the electrolyte in the process is weakened, so that the decomposition of the electrolyte on the surface of the high-voltage material is weakened and inhibited, the deposition of electrolyte decomposition products on the positive electrode side is reduced, and the circulation of the battery is more stable.