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Feed additive composition

Inactive Publication Date: 2015-08-06
DUPONT NUTRITION BIOSCIENCES APS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention relates to a combination of enzymes and specific bacteria that can improve the conversion of plant material into short-chain fatty acids (SCFAs) in the gastrointestinal tract of animals. The combination of xylanase and β-glucanase can improve the solubilization of fibre from the diet, which can lead to better animal performance. The use of specific bacteria, such as Bifidobacterium animalis, can also optimize the conversion of plant material into SCFAs. The resulting increase in the energy value of the plant material can be further enhanced by combining xylanase, β-glucanase, and other fibre degrading enzymes with specific bacteria that produce SCFAs. The use of DFMs that produce SCFAs can also help to increase the population of fibrolytic microflora in the GIT, which can further enhance the conversion of plant material into SCFAs. Overall, this combination of enzymes and bacteria can improve the efficiency of plant material utilization in animals and optimize animal performance.

Problems solved by technology

This solubilisation of hemicellulose would not always be sufficient to increase performance because C5-sugars released are not an efficient source of energy for animals when they are absorbed (Savory C., J. Br. J. Nut. 1992, 67: 103-114), but they are a more efficient source of energy when converted into short chain fatty acids (SCFA) either by microorganisms in the GIT or by DFMs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Responses of Broiler Chickens Fed Wheat-Based Diets Containing Xylanase, β-Glucanase and Direct Fed Microbials

Material and Methods

[0551]The use of animals and experimental protocol was approved by the Institutional Animal Experiment Committee. A diet was formulated to be balanced for energy and nutrients for young broiler chicks (0-21 days of life) (Table 1, Diet I). The cereal component of the diet was either wheat, barley, rye, wheat middlings, wheat bran or combinations thereof whilst the protein component was soybean meal and the source of fat was rapeseed oil. No synthetic antimicrobials or anti-coccidial drugs were included, and the diet was supplied as a mash. The basal diet was divided into portions and the respective enzymes and DFMs added to constitute experimental diets identified in Table 2.

[0552]Each supplement was provided in a premix and added to the mixer during diet preparation. Diets containing the DFM were mixed first and the mixer was flushed between each diet to...

example 2

Effect of 2 Xylanases and Other Fibre Degrading Enzymes (FDE-Mix) and DFM (Bacillus Based Direct Fed Microbial; Lactobacillus Based Direct Fed Microbials when Fed Singly or in Combination on Growth Performance and Cecal Volatile Fatty Acids in Young Broiler Chickens Fed Corn-Based Diets

experiment 1

Material and Methods

[0565]The use of animals and experimental protocol is approved by the institutional Animal Experiment Committee. The basal diet, as fed, is formulated to be balanced for energy and protein, and to match the requirements for growing birds of this age and genotype (Table 9). The cereal component of the diet is corn, and protein component can be soybean meal with or without other protein ingredients such as canola, rape seed meal, etc. Corn co-products such as DDGS or corn gem meal or corn gluten feed can be included either singly or in combination provided that the diet is formulated to meet the nutrient requirements of the birds being fed. No synthetic antimicrobials or anti-coccidial drugs are included, and the diet is supplied as a mash. A common digestibility marker (Titanium dioxide, chromic oxide or celite) is included at 3 g / kg to allow determination of digestibility of dietary components. The basal diet is divided into portions and the respective enzymes an...

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Abstract

A feed additive composition comprising a direct fed microbial (DFM), in combination with a xylanase (e.g. endo-1,4-β-d-xylanase) and a β-glucanase (and optionally a further fibre degrading enzyme), wherein the DFM is selected from the group consisting of an enzyme producing strain; a C5 sugar-fermenting strain; a short-chain fatty acid-producing strain; a fibrolytic, endogenous microflora-promoting strain; or combinations thereof. The DFM may be selected from the group consisting of: Bacillus subtilis AGTP BS3BP5, Bacillus subtilis AGTP BS442, B. subtilis AGTP BS521, B. subtilis AGTP BS918, Bacillus subtilis AGTP BS1013, B. subtilis AGTP BS1069, B. subtilis AGTP 944, B. pumilus AGTP BS 1068 or B. pumilus KX11-1, Enterococcus faecium ID7, Propionibacterium acidipropionici P169, Lactobacillus rhamnosus CNCM-1-3698, Lactobacillus farciminis CNCM-1-3699, a strain having all the characteristics thereof, any derivative or variant thereof, and combinations thereof and the further fibre degrading enzyme may be selected from the group consisting of a cellobiohydrolase (E.C. 3.2.1.176 and E.C. 3.2.1.91), a β-glucosidase (E.C. 3.2.1.21), a β-xylosidase (E.C. 3.2.1.37), a feruloyl esterase (E.C. 3.1.1.73), an α-arabinofuranosidase (E.C. 3.2.1.55), a pectinase (e.g. an endopolygalacturonase (E.C. 3.2.1.15), an exopolygalacturonase (E.C. 3.2.1.67) or a pectate lyase (E.C. 4.2.2.2)), or combinations thereof.

Description

FIELD OF INVENTION[0001]The present invention relates to methods for improving feed compositions using a specific direct fed microbial in combination with a xylanase and a β-glucanase, and to a feed additive composition comprising a direct fed microbial in combination with a xylanase and a β-glucanase. The present invention further relates to uses and kits.BACKGROUND OF THE INVENTION[0002]Supplemental enzymes are used as additives to animal feed, particularly poultry and swine feeds, as a means to improve nutrient utilization and production performance characteristics. Enzyme blends are available to improve the nutritional value of diets containing cereal grains, soybean meal, animal protein meals, or high fibre food and industrial by-products.[0003]The concept of direct fed microbials (DFM) involves the feeding of live beneficial microbes to animals like chickens or pigs, such that when administered in adequate amounts confer a health benefit on the host. Probiotics is another term...

Claims

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Application Information

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IPC IPC(8): A23K1/00A61K38/47A61K35/747A61K35/742
CPCA23K1/008A23K1/009A23K1/1653A23K1/1826A23K1/184C12Y302/01021A23Y2220/00A61K35/742A61K35/747A61K38/47C12Y302/01008A23K1/1893A23K10/16A23K10/18A23K20/189A23K50/75A23K50/30A23K50/60C12N9/2402A23V2400/11
Inventor ISAKSEN, MAI FAURSCHOUBERNARDEAU, MARIONMILLAN, LUIS FERNANDO ROMEROKIARIE, ELIJAH GITUANJAHARENT, SUSAN LUNDNURMINEN, PIIIVI HELENAFORSSTEN, SOFIAPETRI, DANIELGALBRAITH, ELIZABETH ANN
Owner DUPONT NUTRITION BIOSCIENCES APS
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