107 results about "Pectate lyase" patented technology

The invention relates to a dispensing system for releasing preparations in the interior of a washing machine, comprising a dispenser and a cartridge couplable to the dispenser, in which the cartridge comprises at least three chambers, which contain different flowable preparations, in which the first chamber stores at least one enzyme, selected from the group of amylases, mannanases, cellulases, lipases and/or pectate lyases and at least one surfactant and/or complexing agent, the second chamber stores at least one protease and at least one surfactant and/or complexing agent, the third chamber stores at least one scent and/or one optical brightener.

The present invention relates to variants of a cell-wall degrading enzyme having a beta-helix structure, which variant has at least one substituent in a position determined by identifying all residues potentially belonging to a stack; characterizing the stack as interior or exterior; characterizing the stack as polar, hydrophobic or aromatic/heteroaromatic based on the dominating characteristics of the parent or wild-type enzyme stack residues and/or its orientation relative to the beta-helix (interior or exterior); optimizing all stack positions of a stack either to hydrophobic aliphatic amino acids, hydrophobic aromatic or polar amino acids by allowing mutations within one or all positions to amino acids belonging to one of these groups; measuring thermostability of the variants by DSC or an application-related assay such as a Pad-Steam application test; and selecting the stabilized variants. Variants of a wild-type parent pectate lyase (EC 4.2.2.2) having the conserved amino acid residues D111, D141 or E141, D145, K165, R194 and R199 when aligned with the pectate lyase comprising the amino acid sequence of SEQ ID NO: 2 are preferred.

The invention relates to a preparation and application of unsaturated pectic oligosaccharide and a compound biological preservative combined with the unsaturated pectic oligosaccharides, which is characterized in that: pectin is extracted from pericarps or fruit residues, pectate lyase produced by the fermentation of aspergillus niger-wz003 is added into the pectin, and then the unsaturated pectic oligosaccharide is obtained by centrifugal separation, monosaccharide removal by yeast, decoloration by active carbon, polyether sulfone membrane and membrane separation of regenerated cellulose, and the unsaturated pectic oligosaccharide can be obtained by a further drying step. The preparation method of unsaturated pectic oligosaccharide and a compound biological preservative combined with the unsaturated pectic oligosaccharides has the advantages that: self-made pectate lyase is utilized, so the raw materials are easy to obtain and the cost is low; meanwhile, the pectic oligosaccharide and the compound biological preservative has the advantages of innocuity, high efficiency, broad spectrum and wide application range and can reduce the addition of chemical preservatives.

The invention belongs to the technical field of biology and in particular provides a pectate lyase (PL) gene Pcpel1 which is cloned from phytophthora capsici and protein preparation technology thereof. Gene and protein levels prove that the gene is effectively involved in the process that the phytophthora capsici infects hot pepper hosts and results in occurrence of the course of diseases on hot pepper leaves. Plant pathology and cytochemistry technology further prove that after the protein coded by the gene is inoculated onto the hot pepper leaves, obvious withering and shrinking occur on theinoculated parts of the leaves and the cell walls on the affected parts of the leaves are obviously degraded, namely the gene code is an important protein related to the course of diseases or is possibly an important target pathogenic gene of a phytophthora capsici PL gene cluster. The invention provides important technical reserve for further developing phytophthora capsici molecule detection technology.

The invention relates to a process for preparing polysaccharide and application of the polysaccharide, in particular to a process for preparing modified pectin (MPHB) with high bioavailability and low molecular weight and application of the modified pectin in the antitumor field. The process comprises the following steps of: mixing pectin and water, heating, and swelling; cooling, regulating the pH value by using carbonate solution, reducing the degree of esterification by using pectinesterase (EC 3.1.1.11), and raising the temperature to inactivate the pectinesterase; and cooling, degrading a main chain of the pectin by using polygalacturonase (EC 3.2.1.15) or pectate lyase (EC 4.2.2.10), and performing membrane filtration on a product, desalting, concentrating and drying to obtain the MPHB. The process is favorable for preparing the MPHB on a large scale and controlling the molecular weight and molecular weight distribution of the MPHB. The MPHB has the activities of resisting livercancer, cervical carcinoma and the like; and the adding of an intestinal absorption enhancer in an oral formula of the MPHB is favorable for further improving the bioavailability.

The invention discloses alkaline pectate lyase producing gene engineering bacteria and construction and use thereof and belongs to the field of genetic engineering. In the invention, the Escherichia coli pel engineering bacteria capable of secreting and expressing pectinase Pel in vitro with high efficiency are constructed from an industrial prospective by using a totally synthetic Aspergillus nidulans pel gene. The invention also provides a method for producing pectinase by using the engineering bacteria. When the engineering bacteria are used, the fermentation time is short, and at 37 DEG C, the total fermentation lasts for 6 hours, wherein the early fermentation lasts for 4 hours and post fermentation lasts for 6 hours; the enzyme active yield is as high as 400U/mL; in addition, the produced pectinase can be secreted in vitro directly, hybrid proteins are reduced, and the purification is easy. Therefore, the engineering bacteria have better industrial application prospect.

The invention relates to a polymerase chain reaction (PCR) method for specifically detecting pectobacterium carotovorum. A pair of primers is designed according to a base sequence (GenBank:L32171.1) of a pectobacterium carotovorum subsp. carotovorum No.71 strain pectate lyase gene, and a basic local alignment search tool (BLAST) result proves that the pair of primers has high specificity. When the pair of primers is used for PCR detection, bacteria of other genera can be removed, the pectobacterium carotovorum can be distinguished from other bacteria of pectobacterium, and the specificity is high.

The invention discloses a composite enzyme preparation for degumming of a linen fiber raw material, a preparation method of the composite enzyme preparation and a degumming method of the linen fiber raw material. The composite enzyme preparation comprises components in percentage by mass as follows: 70% of alkaline pectate lyase, 2% of alkaline cellulase, 18% of alkaline xylanase and 10% of laccase. With the adoption of the technical scheme, more than 20% of bleaching liquor is reduced in the linen fiber bleaching process, more than 40% of caustic soda is reduced in the linen fiber bleaching process, the breaking strength of the linen fiber is increased by more than 5%, the fineness of the linen fiber is increased by more than 20%, the elongation at break is increased by more than 30%, the fiber strength is uniform, the spinnability of the fiber is remarkably improved, accordingly, economic benefits are increased, and the production cost is reduced; the COD (chemical oxygen demand) is reduced by more than 30%.

The present invention relates to a pectate lyase, a coding gene and applications thereof. According to the present invention, the technical problems of not high activity and poor thermal stability of the existing pectate lyase are solved; the amino acid sequence of the pectate lyase and the nucleotide sequence of the coding gene of the pectate lyase are disclosed, and the preparation method of the coding gene and the applications of the pectate lyase in ramie degumming are disclosed; and the preparation method can be widely used in the pectate lyase preparation field.

The invention discloses a mesona chinensis benth beverage, which is prepared from the following raw materials by weight percent: 55-65% of mesona chinensis benth grains, 2-6% of jumble beads, 1-3% of lotus seeds, 10-15% of white granulated sugar and the balance of purified water; crushing the mesona chinensis benth, adding purified water which is 15-20 times of the weight of the mesona chinensis benth to decoct to boil; cooling and then adding a compound enzyme of cellulose, pectinase and pectate lyase, which is 0.35-0.45% of the weight of the mesona chinensis benth; extracting for 2-4 hours under the conditions that the temperature is 50-60 DEG C and the pH is 5.0-5.8, then adding sodium carbonate or potassium carbonate to adjust the pH to 7.0-8.0, boiling for 2 hours, and filtering to obtain filtrate, namely mesona chinensis benth juice; adding starch and a stabilizer to the mesona chinensis benth juice, evenly mixing, sterilizing at a high temperature and cooling to 40-50 DEG C; soaking for 1-2 hours by using 0.6-1% calcium lactate after dicing, so as to form the mesona chinensis benth grains; and enclosing the mesona chinensis benth grains, the jumble beads, the lotus seeds, the white granulated sugar and the purified water to a container, and sterilizing to obtain the mesona chinensis benth beverage. The mesona chinensis benth beverage disclosed by the invention has abundant mouthfeel, fresh and sweet taste, smooth mesona chinensis benth grains, and sweet, soft and sticky jumble beads and lotus seeds, contains a plurality of vitamins, amino acids and microelements, and is applicable to eating by all people.

The invention discloses strip-shaped mesona jelly. The strip-shaped mesona jelly is prepared from the following raw materials by weight percent: 10 to 20 percent of honey, 20 to 30 percent of starch, 0.5 to 1.5 percent of white granulated sugar, 0.02 to 1 percent of a stabilizer and the balance being mesona juice, wherein the preparation method of the mesona juice comprises the following steps: crushing the mesona, boiling the mesona in pure water with the weight being 15 to 20 times that of the mesona, cooling, adding compound enzyme of cellulose, pectinase and pectate lyase, which accounts for 0.35 to 0.45 percent of the weight of the mesona, extracting the mesona for 2h to 4h under the conditions that the temperature is 50 to 60 DEG C and the pH value is 5.0 to 5.8, adding sodium carbonate or potassium carbonate to adjust the pH value to 7.0 to 8.0, boiling the extract for 2h, and filtering the extract solution, wherein the filter liquid is the mesona juice; and uniformly mixing the mesona juice with starch, the white granulated sugar and the stabilizing agent, sterilizing the mixture at a high temperature, cooling the mixture to 40 to 50 DEG C, adding the honey, uniformly mixing the mixture with the honey, cooling the mixture, cutting the mixture into strips of the needed specifications, and calcificating the strips for 1h to 2h by utilizing 0.6 to 1.2 percent of calcium lactate to obtain the strip-shaped mesona jelly finished product. The strip-shaped mesona jelly is tender in taste, good in flexibility, sweet in taste, rich in honey fragrance, and suitable for different people to eat, with high content of active ingredients.

The invention discloses a preparation method for compound radix isatidis granules. The preparation method comprises the steps as follows: firstly, adding water in radix isatidis raw and decocting twice with the first time and the second time being 2 hours and one hour respectively, merging the decocted soup and filtering the soup; secondly, concentrating the filtered soup with the relative density being 1.10, at 45 DEG C, adding complex enzyme consisting of pectinase, cellulase and pectate lyase for enzymolysis, wherein the weight ratio of the pectinase to the cellulase to the pectate lyase is 5 to 3 to 2, and the addition amount of the complex enzyme is 0.5-1.0% of the weight of the concentrated filtered soup; thirdly, concentrating continuously till the relative density being 1.20, at 45 DEG C, filtering, cooling, adding 95% ethyl alcohol so as to enable the alcohol content to be 30%, leaving to stand for sedimentation, and extracting supernatant liquid; fourthly, recycling ethyl alcohol, concentrating the liquid with the relative density being 1.20 at the temperature of 70 DEG C; fifthly, adding regular amount of cane sugar and dextrin, preparing granules, and drying the granules. According to the preparation method provided by the invention, through adopting the enzymolysis technology additionally, the ethanol concentration during ethanol sedimentation is reduced, and the cost is lowered.

The invention discloses a mesona chinensis benth jelly, which is prepared from the following raw materials by weight percent: 0.5-1% of mesona chinensis benth gelatine powder, 2-5% of native starch, 0.5-1.5% of modified starch, 1-4% of white granulated sugar, 1-4% of cellulose, 0.005-0.012% of sucralose, 2.0-4.0% of honey, and the balance of purified water. The preparation method of the mesona chinensis benth gelatine powder comprises the following steps: crushing the mesona chinensis benth, adding purified water which is 15-20 times of the weight of the mesona chinensis benth to decoct to boil; cooling and then adding a compound enzyme of cellulose, pectinase and pectate lyase, which is 0.35-0.45% of the weight of the mesona chinensis benth; extracting for 2-4 hours under the conditions that the temperature is 50-60 DEG C and the pH is 5.0-5.8; adding sodium carbonate or potassium carbonate to adjust the pH to 7.0-8.0, boiling for 2 hours and filtering to obtain filtrate, namely mesona chinensis benth juice; and baking and grinding in an oven at 90 DEG C after concentrating the mesona chinensis benth juice, so as to obtain the powder, namely the mesona chinensis benth gelatine powder. The mesona chinensis benth jelly with low sugar and high fiber disclosed by the invention is smooth in mouthfeel, fresh and sweet in taste, abundant in honey fragrance, high in content of effective components, low in sugar content, and high in cellulose content, and the product is more abundant in healthcare function, and applicable to eating by all people.

The present invention relates to pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) (amino acids 236–242 of SEQ ID NO: 2) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase may be derivable from Bacillus species.

Using site-directed mutagenesis to mutate the Xanthomonas campestris pectate lyase gene, variants of Xanthomonas campestris pectate lyase with improved thermostability and/or enzymatic activity have been expressed in Escherichia coli, and then isolated and purified. The mutant Xanthomonas campestris pectate lyases are more effective than the wild-type enzyme, also expressed in E. coli, in removing pectic compounds from natural hemp fiber.

The invention provides a preparation method of honeysuckle beverage. The preparation method of the honeysuckle beverage comprises the following steps: providing a honeysuckle extract; providing a mint extract, providing a sugar source, mixing the sugar source and a composite enzyme preparation which is formed by glucolase, maltase, pectinase and pectate lyase, and performing enzymolysis to obtain a sweetening agent; and mixing the honeysuckle extract, the mint extract and the sweetening agent according to a weight ratio of 100:(1-10):(0.1-5) to obtain the honeysuckle beverage. According to the preparation method of the honeysuckle beverage, provided by the invention, the bitterness of the honeysuckle is shielded by adding the sugar source and the mint extract, and the added sugar source is decomposed into micromolecular sugar by the composite enzyme preparation, so the sweetness is reduced and the honeysuckle beverage is suitable for most people to drink.

A structured aqueous liquid detergent composition comprising: at least 10 wt% water, at least 0.5 wt% surfactant, at least 0.0001 wt% of cellulase and /or pectate lyase an external structurant, characterised in that the external structurant comprises at least 0.15 wt%, preferably at least 0.2%, apple fibre that has been mechanically pulped and swollen in water to an extent that it can absorb at least 10 times its own dry weight of water.

The invention discloses an alkaline pectate lyase (PelA) as well as an encoding gene and application thereof. The amino acid sequence of the alkaline PelA is shown by SEQID No. 1. The alkaline PelA is a new PelA obtained by cloning in edible straw mushrooms, has the optimum temperature of 60 DEG C and the optimum pH of 10.0, has good temperature and pH stability, and is high in catalytic performance under the condition that exogenous calcium ions do not exist, thus having good application prospect in the industrial application. Furthermore, the alkaline PelA is derived from the edible mushrooms, thus being safe and reliable; therefore, the alkaline PelA is widely applied to the industries such as food, feed and medicine.

The invention provides preparation of a compound enzyme rich in acidic pectinase as well as a strain and application of the compound enzyme. A cultured fermentation product contains pectinase, xylanase, mannase, acid protease, cellulase, amylase and beta-glucanase. Aspergillus niger is named as aspergillus niger BAK200345, and the preservation number of the aspergillus niger is CGMCC No.19614. Thecompound enzyme rich in acidic pectinase provided by the invention is rich in pectinase types, and pectinesterase, pectate hydrolase, pectate lyase, polygalacturonase and rhamnogalacturonase exist, so that the compound enzyme is a complete pectinase system. The enzyme activity of the acidic pectinase is relatively high and can reach 132960 U/g at most; the compound enzyme can reduce the feed-eggratio, increase the laying rate of laying hens, reduce the excrement sticking phenomenon and improve the intestinal health, and is good in application effect.

A new group of pectate lyases containing the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP), this group of enzymes belongs to the first family of polysaccharide lyases, in industrial processes such as laundry under neutral or alkaline conditions And textile treatment, etc. have a good effect. The pectate lyase may be derived from a Bacillus species.

The present invention relates to a denim garment desizing refining finishing agent and a preparation process thereof. The finishing agent includes the following raw material ingredients by weight: 400-500 parts of a wide-temperature desizing enzyme, 50-100 parts of pectate lyase, 300-400 parts of alcohol ether, 0-3 parts of a defoamer and 50-250 parts of deionized water. Compared with the existingtechnology, the denim garment desizing refining finishing agent can still play high efficiency in the use of low temperature, and is more energy-saving and environmental-friendly, and the denim garment desizing refining finishing agent has a good impurity removal effect and better hand effect.