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Novel pectate lyases

A pectate lyase and amino acid technology, applied in the field of microbial pectate lyase, can solve optimization, content and other problems

Inactive Publication Date: 2001-02-28
NOVO NORDISK AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These microorganisms also frequently produce cellulases and / or hemicellulases, complex multicomponent enzyme preparations derived from these microorganisms can be difficult to optimize for various applications, and they may even contain enzymes with deleterious effects

Method used

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Examples

Experimental program
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Effect test

Embodiment 2

[0325] At Danish company DAKO, rabbit polyclonal monospecific serum against highly purified pectate lyase was raised using conventional techniques. Serum and the pectate lyase of the invention form a single well-integrated precipitate in the agarose gel. Example 2 Cloning, expression, purification and identification of pectate lyase from Bacillus licheniformis

[0326]The construction of the Bacillus licheniformis ATCC14580 genome library was carried out as described in Example 1 for B. agaradhaerens. The pectate lyase-positive clone of the Bacillus licheniformis ATCC14580 gene library was deposited as DSM11789. The pectate lyase-encoding DNA from Bacillus licheniformis ATCC14580, SEQ ID NO:3, was identified after primer walking on the plasmid of E. coli DSM11789. Subcloning in Bacillus subtilis

[0327] The DNA coding sequence of the pectate lyase (amino acid sequence shown in SEQ ID NO: 4) of the present invention is amplified by PCR primer set of the following two oligon...

Embodiment 3

[0347] Cloning, expression, purification and identification of pectate lyase from Bacillus sp. KJ59, DSM12419

[0348] The DNA sequence encoding the mature portion of pectate lyase (SEQ ID NO: 7) was subcloned in Bacillus subtilis.

[0349] Using exactly the same procedure as described in Example 1, the coding DNA sequence of the mature part of pectate lyase encoded by SEQ ZD NO: 7 was cloned and expressed in the pMOL944 / PL2306 expression system. The only difference is the use of the following two PCR primers and the use of genomic DNA isolated from Bacillus sp. KJ59, DSM12419:

[0350] #145375

[0351] 5′-CAT TCT GCA GCC GCG GCA AAT ACG CCA AAT TTC AAC TTA CAAG-3′

[0352] #145376

[0353] 5′-CAG CAG TAG CGG CCG CTT ACG GTT GGA TGA CAC CAA CTC-3′

[0354] The resulting Bacillus subtilis clone expressing pectate lyase was named MB888. The cloned DNA sequence was expressed in Bacillus subtilis, and the protein appearing in the supernatant corresponded to the mature protein...

Embodiment 5

[0372] Cloning, expression, purification and characterization of pectate lyase from Bacillus sp. AAI12 Subcloning of the DNA sequence encoding the mature part of pectate lyase in Bacillus subtilis (SEQ ID NO: 5)

[0373] Using exactly the same procedure as described in Example 1, the DNA sequence encoding the mature portion of pectate lyase (SEQ ID NO: 5) was cloned and expressed in the pMOL944 / PL2306 expression system. The only difference is the use of the following two PCR primers, and genomic DNA isolated from Bacillus sp. AAI12: #80501D1C125′-CAT TCT GCA GCC GCG GCA GCA TCA TTT CAG TCT AAT AAA AAT TATC-3′ #80501D1B125′-GAC GAC GTA CAA GCG GCC GCG CTA CTG TAC AAC CCC TAC ACC-3′

[0374] The resulting Bacillus subtilis clone expressing pectate lyase was named MB644. The cloned DNA sequence was expressed in Bacillus subtilis, and the protein appearing in the supernatant corresponded to the mature protein portion of the mature protein shown in SEQ ID NO:6. Purification and I...

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Abstract

A new group of pectate lyases containing the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP), this group of enzymes belongs to the first family of polysaccharide lyases, in industrial processes such as laundry under neutral or alkaline conditions And textile treatment, etc. have a good effect. The pectate lyase may be derived from a Bacillus species.

Description

[0001] The present invention relates to microbial pectate lyases; more particularly microbial enzymes that exhibit pectate lyase activity as their predominant enzymatic activity in the neutral and alkaline pH ranges; the invention also relates to the preparation of Methods of these enzymes; and methods of using these enzymes in the textile, detergent and cellulosic fiber processing industries. Background of the invention [0002] Pectin polymers are important components of plant cell walls. Pectin is a heteropolysaccharide whose backbone consists of alternating homogalacturonans (smooth regions) and rhamnogalacturonans (hairy regions). The smooth region is a linear polymer of 1,4-linked α-D-galacturonic acid. Galacturonic acid residues can be methylated to varying degrees at the carboxyl group, usually in a non-random manner to fully methylate the polygalacturonic acid region. [0003] Pectinases can be classified according to their preferred substrates (highly methylated pe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/84C11D3/386C12H1/12C12N1/15C12N1/19C12N1/21C12N5/10C12N9/40C12N9/88C12N11/18C12N15/09C12R1/07D06L4/40
CPCC12N9/88D06L3/11C12N9/2465D06L4/40
Inventor L·N·安德森M·舒莱恩N·E·K·兰吉M·E·布约恩瓦德S·莫勒S·O·S·格拉德M·S·考比南K·施诺尔L·康格斯巴克
Owner NOVO NORDISK AS
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