257 results about "Microbial enzymes" patented technology

The invention describes a process for preparing acetone starting from acetyl-coenzyme A comprising process steps A. enzymatic conversion of acetyl-CoA into acetoacetyl-CoA B. enzymatic conversion of acetoacetyl-CoA into acetoacetate and CoA and C. decarboxylation of acetoacetate to acetone and CO₂, which is characterized in that the coenzyme A is not transferred in process step B to an acceptor molecule. In addition, process step B is surprisingly catalysed by enzymes of the classes of acyl-CoA thioesterase, acyl-CoA synthetase or acyl-CoA thiokinase.

A completely novel metabolic pathway is concerned, because the enzymatic hydrolysis of acetoacetyl-CoA without simultaneous transfer of CoA to a receptor molecule has never previously been described for any microbial enzyme.

A process for preparing the multi-vitamine multi-enzyme high-protein microbial preparation by the solid fermentation of multiple bacterial strains includes such steps as preparing seed culture media, respectively inoculating reticularia, torula yeast, glutamic corynebacteria, Bacillus subtitis, Aspergillus niger, etc into said seed culture media, culturing at 30-35 deg.C for 15-18 hr, inoculating them into the solid culture medium, fermenting at 33+/-0.5 deg.C for 70-80 hr, and fast drying. Its can be used as the feed containing living microbes, microbial enzymes, vitamines, etc.

The invention provides a method for improving the quality of expanded cut rolled stem of tobacco by microbial enzyme. The procedures are as follows:1) Preparation of bio-enzyme: (1) Aspergillus niger is activated and cultured by potato cane sugar culture medium and is then transferred into sterilized seed culture medium, and shake cultivation is carried out at the temperature of 28 DEG C for 24 hours with 250r/min<-1>; seed liquid with 10% of inoculation amount is transferred into a sterilized 500ml shake flask (containing 100ml culture liquid for enzyme), and the seed liquid carries out incubation for culturing at the temperature of 25 DEG C for 96 hours and then zymotic liquid is obtained. (2) Extraction of lignin degradation complex enzyme: After being filtered by filter paper, the zymotic liquid is centrifugally separated at the low speed of 1500rpm. The supernatant carries out fractional salting out by (NH4)2SO4 till the saturation is 0.7 to obtain crude enzyme liquid, after the crude enzyme liquid carries out ultrafiltration dialysis, the lignin degradation complex enzyme is obtained. Activities of enzyme components: 350U/L of Lip, 11U/L of MnP and 5.6U/L of Lac; 2) Improvement of the quality of expanded cut rolled stem of tobacco: The expanded cut rolled stem for cigarette is weighed and sprayed evenly by lignin degradation complex enzyme liquid containing 0.2-0.4% of expanded cut rolled stem after being diluted by distilled water at the temperature of 27 DEG C-29 DEG C. The expanded cut rolled stem of tobacco is placed in a sealed container for enzymolysis for 95-97 hours and dried naturally till the moisture content is 12.5%-15%. The result by subjective analysis suggests that the complex enzyme preparation can effectively decrease the lignin content of the expanded cut rolled stem, promote the transformation of aroma matter and improve aroma quality and taste.

The invention discloses a method for repairing cement-based material cracks, as well as a culture solution and a repair nutrient solution. The method for repairing cement-based material cracks by through microorganisms comprises the following steps that: a Bacillus pasteurii strain is inoculate onto a culture medium provided with a urea-containing substrate; shake cultivation is carried out at a temperature of between 25 and 37 DEG C, and then a culture bacteria solution is taken out and centrifuged and has supernatant fluid removed; strain cells are collected through the culture solution; the concentration of the strain cells is controlled in a range of between 2x10<9> and 1x10<11> cell/ml; standard sand, urea and Ca(NO3)2.4H2O mixture are added to each milliliter of strain cell solution obtained through collection, mixed, stirred into slurry and injected into cement stone cracks; the frequency of the repair nutrient solution injection is not less than two times; finally, maintenance is carried out. In the culture solution, each liter of culture solution contains 4 to 6 g of peptone, 2 to 4 g of beef extract and 20 to 60 g of urea. The method fully utilizes microbial resources in nature; CO3<2-> decomposed out through microbial enzyme can chelate Ca<2+> in a substrate so as to be mineralized and deposit calcium carbonate, and is close in the combination with the substrate and good in stability.

The invention relates to a method used microorganism enzyme resolution DL-pantoic acid lactone to produce D-pantoic acid. It uses the D-pantoic acid lactone hydrolase strain of the fusarium, gibberella, aspergillus, penicillium, rhizopus, gliocladium, aureobasidium to ferment and culture, uses wet thallus as coarse enzyme, DL-pantoic acid lactone as substrate to produce D-pantoic acid. L- pantoic acid lactone can be reclaimed. The DL-pantoic acid lactone gained by racemization reaction can newly be used to do resolution.

To provide a method for preparing optically active compounds, which mainly contain (R)-amino compounds, by microbial enzymes efficiently and inexpensively; a polypeptide having stereoselective transaminase activity which can be suitably used for the above preparation method; and a DNA encoding the polypeptide. A method for preparing an optically active amino compound, characterized in stereoselectively transaminating by acting a transaminase on an amino group acceptor, a ketone compound in the presence of an amino group donor, a primary amine; a DNA comprising a nucleotide sequence encoding a polypeptide having stereoselective transaminase activity; and a polypeptide having stereoselective transaminase activity obtainable from a culture of a microorganism belonging to the genus Arthrobacter.

The invention provides a microbe-enzyme composite preparation used for restoring water in an urban and rural polluted river, and its preparation method. The urban and rural polluted river restoration microbe-enzyme composite preparation is characterized in that the microbe mainly comprises 30-60% of Bacillus licheniformis, 25-35% of a nitrobacterium, and 15-35% of pseudomonas putida; and the composite enzyme mainly comprises 40-60% of a protease, 15-25% of an amylase and 25-35% of a cellulase. The optimized microbe is compounded with the composite enzyme to prepare the microbe-enzyme composite preparation against water of different pollution degrees, the addition amount of the microbe is 1-5g/m<3>, and the addition amount of the composite enzyme is 0.01-0.05g/m<3>. The microbe-enzyme composite preparation is added to the urban and rural polluted river to carry out microbial reinforced restoration with aeration as an auxiliary measure, can effectively improve defects of low microbe metabolism activity, low environmental tolerance, short retention time in the polluted water and the like existing in the prior art, and has a substantial water purifying effect.

The present invention discloses a mixed enzyme production process by solid fermentation of fruits and vegetables and microorganisms and the production process includes the following steps: step one, washing and cleaning fresh fruits and vegetables; step two, juicing the cleaned fruits and vegetables using a juice extractor and obtaining the fruit and vegetable juice and the fruit and vegetable residue; step three, adding water content adjusting raw materials according to different water content of the obtained fruit and vegetable residue to adjust the water content of the fruit and vegetable residue, and mixing the above materials evenly to obtain a base material; step four, putting the step three obtained base material into a fermentation bottle to conduct sterilization; step five, cooling the sterilized fruit and vegetable base material to room temperature, and inoculating microorganism liquid strains to conduct solid fermentation; and step six, after the end of solid fermentation, blending the obtained fruit and vegetable juice into the solid fermented materials, conducting low temperature drying, and thereby obtaining the fruit and vegetable and microorganism mixed enzymes containing both fruit and vegetable enzymes and microbial enzymes. The mixed enzymes can not only fully preserve the enzyme activity of fruits and vegetables, but also obtain the enzyme ingredients of microorganisms, and in addition, the production process can also achieve the full advantage of the entire fruits and vegetables, does not produce any waste, and is ecological and environmental protective.

The invention discloses a method for improving the quality of cabo, comprising the following steps: dissolving microbial enzymes in a citric acid solution, uniformly spraying the completely dissolved enzyme solution on the cabo, treating the cabo sprayed with enzyme solution under constant temperature and humidity, and drying, etc. The invention uses the microbial enzyme technique to strengthen the fermentation treatment of the cabo, which reduces the wood flavor of the cabo, improves the irritation caused by undesirable substances such as cellulose. The improved cabo facilitates the process of the subsequent procedures and is added into the group prescription of the tobacco leaves, which increases the comfort of the oral cavity, decreases miscellaneous gases, softens the smoking gas, reduces the content of harmful substances in the cigarette and improves the safety of smoking. The invention has the advantages of simple and easy practice and strong practicality, which is beneficial to reducing the consumption of tobacco leaves and save tobacco leaf resources.

The invention provides a method for oriented biosynthesis of GAMG, and belongs to the technical fields of biosynthesis and biotransformation. The method comprises the following steps: producing corresponding beta-D-glucuronidase by penicillium purpurogenum stoll in a fermentation medium; adding the obtained beta-D-glucuronidase into conversion solution containing glycyrrhizic acid substrate, orientedly hydrolyzing the glycyrrhizic acid under the action of enzyme, and obtaining a coarse product of GAMG; and performing organic solvent extraction, resin adsorption and purification to obtain a GAMG product. The method takes microbialenzyme as a catalyst, and has high reaction efficiency, no pollution, simple steps, and low cost; and the product is single and has high purity.

The invention provides a cleaning cosmetic composition which comprises 0.5-5.0% of microbial enzymes by weight and 95.0-99.5% of common cosmetic additives by weight. The cleaning cosmetic composition provided by the invention realizes maximum effective rate of 87.72% and minimum cleaning efficiency of 81.40%, thereby being a good skin care product.

The invention discloses an environment-friendly microbial method garbage treatment device. The device comprises a treatment box, the upper part and the lower part of the treatment box are sequentiallyinstalled with a crushing cylinder, a reaction pot and a product classification and purification device through a grinding chamber, a microbial reaction chamber and an enzymolysis product treatment chamber; a rotary grinding cutting device is arranged in the crushing cylinder, a uniform rolling stirring device is arranged in the reaction pot, efficient crushing of the rotary grinding device enables the garbage particles to be more finely crushed, the speed of organic matters in the microbial enzymolysis waste is higher, the uniform rolling stirring device can enable the garbage to be fully and uniformly contacted with microorganisms, the area and the toxicity killing efficiency of the primary purification of the garbage are improved; the method comprises the following steps of crushing and grinding the garbage by the rotary grinding cutting device, then the garbage enters the reaction pot, the microorganisms in the reaction pot carry out enzymolysis reaction on the crushed garbage forthe first time, the crushed garbage is uniformly mixed with the microorganisms, the enzymolysis reaction efficiency is high, and the garbage purification treatment is more thorough.

Shark protein antigypertensive peptide, preparation and application thereof, which pertains to marine biology technical field. The novel antigypertensive peptide with two amino acid separated and purified from proteolysis product of shark, has a high inhibiting activity for angiotensin converting enzyme (ACE). Preparation includes steps of carrying out enzymolysis for shark protein to prepare enzymatic hydrolysate, seperating and purifying peptide from the enzymatic hydrolysate, and sequencing of peptide. The invention is applicable for preparing antihypertensive medicament. The invention apply microbial enzyme technology to deep development of shark, obtain peptide having novel sequence, with high inhibiting activity for ACE.

Melamine is a common industrial chemical contaminant which should be absent from food and feed supplies due to melamine's toxicity. Provided is a method to assess the presence of melamine in samples prepared from compositions. The method may include using a microbial enzyme called melamine deaminase which hydrolyzes melamine to ammeline and ammonia. The method may include assessing the presence of any ammonia produced from an enzymatic reaction between the sample and the enzyme.

The invention provides a preparation method of algae extract. The preparation method comprises the following steps: impurity removal and cleaning, mincing, homogenization, pH regulation, pressurization and alkaline hydrolysis, fermentation and degradation of compound microbial enzyme and filtration, wherein a filtrate product is algae extracting liquid, and filter residue can be directly compounded into a soil conditioning agent or is dried and prepared into a algae-extract dry-powder product. The active algae extract prepared by the invention has the beneficial effects that the components such as minerals, alginic acid, algae polyphenol, mannitol and beneficial element are retained, protein and polysaccharide components in algae are degraded, and the absorption is easy; beneficial microbes and various enzymes are added, and various microbial secondary metabolites can be generated, so that the biological activity is better.

The invention relates to ginseng endogenesis zygorhynchus moelleri mildew (CGMCC (China General Microbiological Culture Collection): No.4315). The mildew has the capability for preparing Rd by converting a substrate ginsenoside Rb1 and has higher substrate unicity and product unicity, and the preparation of the ginsenoside Rd by utilizing the mildew can adopt in-site conversion. A method for preparing the ginsenoside Rd comprising the following steps of: dibbling the mildew in a PDA (Potato Dextrose Agar) culture medium containing the ginsenoside Rb1, standing still at 25 DEG C and culturing for 5-7 days or vaccinating the mildew on an enzyme production culture medium by adopting a microorganism enzymic method and culturing at 28 DEG C for 5-7 days, collecting enzyme liquid, mixing with the ginsenoside Rb1 and reacting at 40 DEG C for 24h. The ginsenoside Rd produced by adopting the technical scheme of the invention has the advantages of strong specificity, simplicity, convenience, safety, reliability and low cost without side products, the purity of the fermentation product Rd is higher than 90 percent, and the conversion rate can be higher than 60 percent.

The invention relates to the utilisation of fatty materials with substantial free fatty acid content in the production of biodiesel by the use of microbial enzymes that are effective in a solvent-free process for the production of esters of fatty acids and C₁-C₃ alkyl alcohols.

The invention discloses a bacterial strain for producing laccase, a method for producing laccase by the bacterial strain, the produced laccase and application of the laccase, and relates to the field of microbial enzyme. The bacterial strain for producing laccase is trametes pubescens (Trametes pubescens BJFC-C1108) with a microbial preservation number of CGMCC No. 10308. By utilizing the trametes pubescens (Trametes pubescens BJFC-C1108), the yield of the produced laccase is relatively high; the enzyme activity can be up to 28.687 U/mL; the prepared laccase has relatively high activity, stability and tolerance under the alkali condition (pH 7.0-10.0), has relatively high tolerance for metal ions, can be used for shortening the time required by complete decoloring of azo dye Congo red to a certain extent, has a potential application value, can be applied to more industrial fields and has favorable development and application prospects.

The invention relates to a granular material for produing bob veal, and the granular material consists of corn, bran, whey powder, wheat albumen powder, peanut albumen powder, lysine, methionine, threonine, tryptophan, a compound microbial enzyme preparation, a vitamin complex and a mineral substance compound. Tests show that after calves are fed with the granular material provided by the invention, the granular material and milk powder substitutes can be used together to produce veal with high quality, and on the premise of not influencing the performance of the produced veal, the disease incident of calves can be reduced, and the health status of the calves can be improved.

The invention provides a composite of a slow-release soil moisture preserving microbial enzymes organic compound fertilizer and also provides a method for preparing the compound fertilizer of the composite and application of the slow-release soil moisture preserving microbial enzymes organic compound fertilizer in agricultural production. The slow-release soil moisture preserving microbial enzymes organic compound fertilizer provided by the invention has the advantages of bidirectional regulation on promoted release and slow release and double virtues of moisture preservation and fertility preservation, particularly has low cost, and can be widely used for the agricultural production, in particular to the agricultural production in arid regions.

The invention discloses a method for preparing D-cystine and L-tryptophane by using DL-cysteine split by a microbial enzyme method. The method comprises the following steps of: splitting the DL-cysteine to produce D-cysteine and L-tryptophane through an enzymatic reaction by using fermentation liquor of recombined Bacillus subtillis which efficiently express tryptophanase as an enzyme source and using the DL-cysteine and indole as a substrate; and oxidizing the enzymatic reaction liquor to ensure that the D-cysteine is oxidized into D-cystine, then regulating pH to be 5 and performing isoelectric point crystallization to separate the D-cystine out, and collecting the precipitation to obtain the D-cystine. A method for purifying the L-tryptophane comprises the following steps of: removing the residual indole from the supernatant which is subjected to the isoelectric point crystallization by adopting an S-8 type macroporous resin, then absorbing the L-tryptophane in the supernatant by adopting an NKA-II type macroporous resin, eluting by using 50 percent ethanol, reducing pressure, concentrating and drying to obtain the L-tryptophane. The invention provides a novel process route of green production of the D-cystine and the L-tryptophane.