57 results about "Microbial enzymes" patented technology

The present invention relates to functional foods and dietary supplements comprising a specific diamine oxidase (DAO) enzyme derived from Glutamicibacter halophytocola, uses of said enzyme and respective methods for the production of biogenic amine-depleted products, uses of said enzyme as a biosensor for the analysis of one or more biogenic amine(s), as well as said enzyme for use in medicine, in particular for use in the prevention or treatment of a condition or disease that is associated with increased levels of biogenic amines.

The invention discloses a microbial odor remover suitable for environment and a preparation method thereof, and relates to the technical field of daily chemical products. The preparation method comprises the following steps: adding pediococcus acidilactici and bacillus velezensis into a citric acid-disodium hydrogen phosphate buffer solution according to a ratio, standing and activating, adding bromelain, lipase, rosmarinic acid nanoemulsion, tea polyphenol and citrus polyphenol-EDTA (Ethylene Diamine Tetraacetic Acid) mixture, homogenizing, adding konjac glucomannan-chitosan gel and a trehalose-glycerol compound according to a ratio, stirring, filtering, and drying to obtain a finished product. And subpackaging in a dark place to prepare the microbial odor remover suitable for the environment. Through the synergistic effect of microorganisms, enzymes and a natural deodorization medium, a multi-stage peculiar smell elimination system is constructed, the dual functions of immediate neutralization and continuous bacteriostasis are achieved, the application scene is wide, corrosion and residues to base materials such as fabric and plastic are avoided, and the concentration of harmful gas can be more effectively controlled and pathogenic microorganisms can be inhibited in large-scale breeding application.

This invention relates to the field of low-temperature fermentation formulation technology. The invention provides a low-temperature resistant compound bacterial enzyme formulation, its preparation method, and its application in the co-fermentation of straw and manure. The compound bacterial enzyme formulation comprises the following raw materials in parts by weight: 8-10 parts of compound bacterial agent, 1-1.4 parts of compound enzyme agent, and 88.6-91 parts of excipients. The compound bacterial enzyme formulation of this invention can ferment straw and manure under conventional conditions as well as at low temperatures of around 15°C, effectively meeting the fermentation requirements in low-temperature regions. By combining the excipients with the bacteria and enzymes, substances and nutrients during the fermentation process can be effectively adsorbed, avoiding acid inhibition, and providing attachment sites for the bacteria and enzymes, thus prolonging their activity.

The application relates to a crisp type pre-conditioned food of tussah pupae and a preparation method thereof. The method is characterized in that fresh tussah pupae are used as raw materials, a series of processing technologies such as spraying and moisturizing of organic acid, temperature control, inorganic acid immersion and killing, microbial-enzymatic treatment of tussah pupae, pickling and baking are combined to prepare the crisp type pre-conditioned food of tussah pupae, the method is simple in operation, can change the permeability of the chitin shell of the tussah pupae, increase the migration rate of the seasoning, reduce the proportion of the burst plasma, and simultaneously reduce the oil oxidation caused by repeated baking treatment.

The application provides a low-cost artificial feed for domestic silkworms and a preparation method thereof, and relates to the technical field of domestic silkworm breeding.The low-cost artificial feed for domestic silkworms is composed of mulberry leaf powder, corn powder, organic acid, sitosterol, microorganisms, enzyme preparation, vitamins, minerals and the like, and can effectively improve the stress resistance of the domestic silkworms, and improve the silk production rate of the silkworms and the quality of the silk.

The present invention discloses a strain of Fusarium graminearum (Fusarium graminearum) AY2-19 and its application in extracting total flavonoids from mugwort. Fusarium graminearum AY2-19 is deposited in Guangdong Province Microbial Culture Collection Center, with deposit number GDMCC No: 63062 and deposit date December 20, 2022. Before extracting total flavonoids from mugwort ethanol ultrasonic method, the crude enzyme solution prepared by fermentation of Fusarium graminearum AY2-19 is added. Because the crude enzyme solution contains a variety of hydrolases, it is possible to synergistically hydrolyze substances such as cellulose, hemicellulose and pectin in mugwort, damage the cell wall, contribute to the dissolution of flavonoids, and significantly improve the total flavonoid extraction yield. The method for extracting total flavonoids from mugwort by microbial enzymolysis assisted by the present invention is more directly compared with the use of ethanol ultrasonic extraction method, and the yield of total flavonoids extracted from mugwort can be increased by 25.2%.

The present invention relates to improved microbial enzymes with transfructosylation activity for efficient and cost-effective production of fructo-oligosaccharides. More specifically, the invention is directed towards obtaining mutant FTase family of genes from genus Aspergillus. Nucleic acids, peptide sequences, mutant proteins, vectors and host cells for recombinant expression of novel FTases are also provided. Various mutations, such as but not limited to point mutations and deletion mutations as well as combinations thereof are presented herein. The invention also relates to a process for the expression of a novel recombinant FTase mutants as a secreted protein. The enzymes exhibit high purity after filtration, which eliminates the need for costly chromatographic procedures.

The invention provides a fermented corncob as well as a preparation method and application thereof, and belongs to the technical field of ruminant nutrition and feed science. The fermented corncob is prepared from the following raw material components in parts by mass: 32 to 42 parts of corncob, 2.5 to 5 parts of soybean meal, 3 to 5 parts of cottonseed meal, 2 to 4 parts of wheat bran, 2 to 5 parts of rice bran, 2 to 5 parts of glucose, 2.5 to 5 parts of cane molasses, 0.2 to 0.5 part of expanded urea, 2 to 4 parts of attapulgite powder, 0.4 to 0.6 part of compound bacterium enzyme mixture, 0.3 to 0.5 part of feed-grade sodium chloride, 0.3 to 0.5 part of feed-grade magnesium oxide and 34 to 42 parts of water. The corncob and other raw materials are subjected to mixed fermentation through combination of various bacteria and enzymes, so that the nutritional value and the nutritional level of the feed are improved. The fermented corncob can replace part of corn in the feed, the production performance is improved, the feed cost is reduced, resource recycling is effectively promoted, and the palatability of the feed is improved.

The invention discloses a microbial enzyme synergistic pymetrozine and nitenpyram water dispersible granule which comprises the following components in percentage by weight: 40-70% of a pymetrozine active compound, 10-25% of a nitenpyram active compound, 1-10% of microbial enzyme, 1-15% of a dispersing agent, 1-10% of a wetting agent, 1-10% of a stabilizer, 1-10% of a buffer solution, 2-10% of an adhesive and the balance of a carrier. The invention also provides a preparation method of the composition. Compared with the prior art, the synergistic water dispersible granule has the advantages that the epidermis or digestive system of pests is destroyed by enzyme generated by specific microorganisms, and the pymetrozine and nitenpyram are combined to form the synergistic water dispersible granule, so that the control effect can be improved, the use of chemical agents can be reduced, and the water dispersible granule is short in disintegration time and low in cost. The suspension rate is greater than or equal to 90%, and the suspension can be used for unmanned aerial vehicle flight prevention and adapts to different water quality conditions.

The invention relates to the technical field of feed, and discloses a feed compound microorganism enzymolysis method and enzymolysis device.The method comprises the following steps that S1, a feed base material smashed to 20-40 meshes and a compound enzyme preparation are premixed according to the mass ratio of 100: (0.3-0.8), and a premix is obtained; s2, continuously feeding the premix into enzymolysis equipment, and carrying out enzymolysis reaction for 15-30 minutes under the conditions that the temperature is 35-42 DEG C and the dissolved oxygen value is greater than or equal to 2mg / L; s3, after the enzymolysis is completed, adding a compound microorganism bacterial liquid into the enzymolysis equipment on line, adjusting the pH value of the system to 5.8-6.2, and continuing to ferment for 2-4 hours; and S4, after fermentation is completed, drying the materials at low temperature until the moisture content is less than or equal to 12% to obtain a finished product. Enzymolysis and fermentation processes are organically combined, enzymolysis and microbial fermentation are performed in stages under the condition of strictly controlling temperature, dissolved oxygen and pH, the release rate and bioavailability of nutrient substances in the feed are remarkably improved, and the method has the advantages of short reaction time, high efficiency, high condition controllability, low moisture content of a finished product, easiness in storage and the like.

The invention relates to an automatic multi-temperature-control microplate reader based on integration of a mechanical arm and artificial intelligence (AI), and aims to improve automation and accuracy of microbe growth monitoring and analysis. Operation such as culture dish taking and placing, culture solution adding and timing sampling can be automatically completed through the mechanical arm. The AI algorithm automatically adjusts parameters such as culture temperature, humidity, illumination and the like according to real-time data and environmental conditions so as to optimize the growth environment of microorganisms. Meanwhile, key indexes such as a light absorption value and cell density are monitored, a microorganism growth curve is drawn in combination with AI analysis, and important parameters such as the growth rate and the maximum growth amount are calculated. The AI can also mine potential laws in the data and provide valuable research information. The instrument improves the efficiency, accuracy and reliability of microbiological research, is widely applied to the fields of microbiological growth law research, resource development, disease diagnosis and the like, and promotes related technical progress.

The invention relates to a liquid microbial enzyme as well as a preparation method and application thereof. The liquid microbial enzyme is prepared by taking soybean meal, fish meal, rice husk and water as raw materials, sterilizing and fermenting through a compound microbial agent, and the compound microbial agent comprises bacillus subtilis, bacillus licheniformis and bacillus velezensis. In the application, the soybean meal serves as a core nitrogen source and an energy source, soybean isoflavone of the soybean meal promotes functional bacteria to proliferate and enhances enzyme activity, and a fiber structure can optimize fermentation conditions; the fish meal provides high-quality protein which can be decomposed to generate organic acid, so that the pH value is reduced, and putrefying bacteria are inhibited; the rice husks maintain the material structure, and the porous characteristic improves ventilation and adsorbs peculiar smell; the bacillus velezensis as an auxiliary enzyme source can enhance the variety coverage of soluble enzymes, so that the substrate degradation capacity is more comprehensive. After the liquid microbial enzyme is prepared, the liquid microbial enzyme and the hermetia illucens are combined, livestock and poultry manure is used as a conversion object, and the conversion efficiency of the livestock and poultry manure on the manure is improved by regulating and controlling intestinal microflora of larvae of the hermetia illucens.

The invention belongs to the technical field of biological product extraction, and particularly relates to a bovine-derived Muslim chondroitin sulfate and collagen peptide co-production method based on a composite enzymolysis-membrane separation coupling technology, which comprises the steps of pretreatment of bovine cartilage, enzymolysis, membrane analysis and filtration, extraction of chondroitin and collagen peptide and the like. According to the method, animal source enzyme is replaced by microbial enzyme, religious taboo is avoided, resources are efficiently utilized, two high-value products are synchronously extracted, and the raw material cost is reduced; a membrane separation technology replaces an organic solvent, and pollution is reduced.

The invention discloses a multi-bacteria enzyme composition for water purification treatment and application, and relates to the technical field of water purification. The microbial agent is prepared from the following components in parts by weight: 15 to 30 parts of rhodopseudomonas palustris, 15 to 30 parts of bacillus subtilis, 25 to 35 parts of serratia marcescens, 25 to 35 parts of denitrifying pseudomonas, 25 to 35 parts of lactic acid coccus, 25 to 35 parts of bacillus amyloliquefaciens and 25 to 35 parts of rhodotorula benthica. The synergistic effect of the flora can inhibit the outbreak of harmful algae, promote the growth of beneficial algae, improve the flocculation performance of a water body and effectively improve the transparency. Meanwhile, the composition has a remarkable removal effect on key pollution indexes such as ammonia nitrogen, COD (Chemical Oxygen Demand), BOD (Biochemical Oxygen Demand), suspended solids and total phosphorus, so that the systematic repair of the water body is realized.

Provided herein are methods of analyzing a fecal sample including measuring the amount of ethanol produced by the fecal sample and measuring the presence, absence, or relative abundance of Ruminococcus gnavus, Escherichia coli, or Klebsiella pneumoniae in the fecal sample compared to a reference sample, and / or measuring the expression of genes associated with the heterolactic fermentation pathway, the mixed acid fermentation pathway, the ethanolamine utilization pathway, and / or acetylene degradation pathway. Also provided herein are methods of treating a condition associated with gut alcohol production in a subject including analyzing a fecal sample from a subject, determining the subject has a gut alcohol production condition, and administering to the subject an effective amount of a treatment comprising a probiotic with optional resistant starch, fecal microbiota transplantation (FMT), an antimicrobial agent, a microbial enzyme inhibitor, or a combination thereof.

The invention provides a microbial enzyme catalytic synthesis method of 20 alpha-hydroxyprogesterone, and belongs to the technical field of biosynthesis. The invention establishes an efficient, green and high-selectivity 20 alpha-hydroxyprogesterone biosynthesis method, and the method has the direct effects that the directional hydroxylation of the C20 site of the substrate progesterone is realized by utilizing a recombinase technology, the generation of byproducts is effectively avoided, the subsequent separation and purification process is simplified, the reaction conditions are mild, the cost is low, and the method is suitable for industrial production. The energy consumption and the safety risk are reduced; from the aspect of product application significance, the method provides a stable and reliable material source for large-scale preparation of 20 alpha-hydroxyprogesterone, the environmental protection property and economical efficiency of the production process are remarkably improved, a solid material foundation is laid for in-depth study on the biological activity and application of the compound, and the method is suitable for industrial production. And a beneficial technical reference is provided for green biological manufacturing of other steroid hormone medicines.

The invention relates to the technical field of organic fertilizer production and processing, in particular to a microbial enzymolysis device for organic fertilizer production, which comprises a heating tank, a timing discharging mechanism and a stirring mechanism are arranged on the heating tank, the timing discharging mechanism comprises a plurality of storage tanks, each storage tank is in threaded connection with a cover, and the cover is in threaded connection with the stirring mechanism. Lifting rods are slidably connected to the bottom walls of the storage tanks, linkage balls are fixedly connected to the bottom ends of the lifting rods, lifting plates are fixedly connected to the top ends of the lifting rods, springs are fixedly connected to the bottom ends of the lifting plates, and a plurality of sealing rods arranged in an array mode are fixedly connected to the outer walls of the lifting plates. The method disclosed by the invention has the beneficial effects that the high-solid-content and high-viscosity organic substrate has no standing dead zone and is in full contact with enzyme liquid and microorganisms, and the enzymatic hydrolysate can be uniformly dispersed along with the reaction process, so that not only is the insufficient enzyme amount in a substrate aggregation region avoided, but also the excessive accumulation of the enzyme liquid in a substrate sparse region is prevented, and the stability of the enzymatic hydrolysis reaction is ensured.

The invention belongs to the technical field of bacterium-enzyme synergistic fermentation methods, and particularly relates to a bacterium-enzyme synergistic fermentation method with high fermentation quality, which comprises the following steps: taking fresh moringa oleifera and morinda citrifolia stems and leaves, crushing the moringa oleifera and morinda citrifolia stems and leaves by a cutting machine until the particle size is 1-2cm, drying the crushed moringa oleifera and morinda citrifolia stems and leaves in the shade for 6 hours, and controlling the moisture content of the materials dried in the shade to be 65-75%, an enzyme microbial agent is added to the material dried in the shade and mixed to be uniform, the enzyme microbial agent is composed of lactic acid bacteria, bacillus licheniformis, aspergillus niger, cellulase, phytase and xylanase, and the dosage of the components is that the lactic acid bacteria accounts for 0.15% of the total mass of the material dried in the shade, the bacillus licheniformis accounts for 0.15% of the total mass of the material dried in the shade, the aspergillus niger accounts for 0.15% of the total mass of the material dried in the shade, the cellulase accounts for 0.1% of the total mass of the material dried in the shade, the phytase accounts for 0.1% of the total mass of the material dried in the shade, and the xylanase accounts for 0.1% of the total mass of the material dried in the shade. The materials mixed with the enzyme microbial inoculum are loaded into polyethylene silage fermentation bags, vacuum packaging and sealing are conducted, the sealed polyethylene silage fermentation bags are placed in a shady and cool place, and fermentation is conducted for 28 days.

The application provides a method for preparing vinegar by solid-state fermentation and vinegar prepared by solid-state fermentation. The method for preparing vinegar by solid-state fermentation comprises the following steps: mixing a composite microbial enzyme preparation, wine mash and grain husks to prepare vinegar dregs; the composite microbial enzyme preparation comprises active brewing dry yeast, lactobacillus plantarum, alpha-amylase, glucoamylase and phytase; the vinegar dregs are subjected to closed fermentation; aerobic acetic acid fermentation is performed on the surface of the vinegar dregs after the closed fermentation by inoculating acetic acid fermentation strains until the total acid content of the fermented vinegar dregs no longer increases. The composite microbial enzyme preparation is used in the solid-state acetic acid fermentation, and a segmented fermentation process is adopted; the composite microbial enzyme preparation is used to strengthen the population of solid-state acetic acid fermentation strains and the enzyme system, promote the decomposition and utilization of starch in the bran in the grain husks, increase the content of lactic acid and acetic acid in the solid-state acetic acid fermentation, and then increase the fermentation yield.

This invention relates to a phthalate hydrolase mutant and its application in phthalate degradation, belonging to the field of microbial enzyme technology. The amino acid sequence of the phthalate hydrolase mutant HylD1 is shown in SEQ ID NO.1, and the nucleotide sequence of the encoding gene is shown in SEQ ID NO.2. This enzyme can catalyze the reaction to generate MMP using DMP as a substrate; or catalyze the reaction to generate MEP and EMP using DEP as a substrate. In this invention, the encoding gene of the phthalate hydrolase HylD1 from *Paracoccus koningii* BJQ0001 was artificially modified by site-directed mutagenesis at amino acid position 265, resulting in a mutant HylD1 (R265A) with higher activity. Compared with the wild-type phthalate hydrolase HylD1, the absolute enzyme activity of this phthalate hydrolase mutant HylD1 (R265A) in degrading DMP and DEP is increased by 2-fold and 3.6-fold, respectively, and a special product EMP is also produced during the degradation of DEP.

This invention discloses a *Aspergillus spp.* SLP7-15 and its application in the extraction of polyphenols from pomegranate peel. The fermentation broth obtained from *Aspergillus spp.* SLP7-15 culture is filtered, and the filtrate is mixed with pomegranate peel powder. Enzymatic hydrolysis is performed at 35–40℃ for 4–6 hours, followed by the addition of deionized water and anhydrous ethanol. The mixture is then subjected to ultrasonic extraction, filtered, evaporated under reduced pressure, dissolved in anhydrous ethanol, and vacuum dried to obtain the polyphenol extract. Before the ultrasonic extraction of pomegranate peel polyphenols with ethanol, the addition of crude enzyme solution prepared from *Aspergillus spp.* SLP7-15 fermentation facilitates the dissolution of polyphenols, significantly improving the polyphenol extraction yield. The microbial enzymatic hydrolysis-assisted extraction method for pomegranate peel polyphenols provided by this invention can increase the polyphenol extraction yield by 26.6% compared to the direct ultrasonic extraction method with ethanol.

This invention provides a supported nickel-based catalyst, its preparation method, and a method for catalytic separation of straw components, relating to the field of catalytic materials technology. The supported nickel-based catalyst provided by this invention comprises a bio-based nitrogen-doped carbon support and active metallic nickel supported on the bio-based nitrogen-doped carbon support. The bio-based nitrogen-doped carbon support is obtained by pyrolysis and carbonization of a mixture of straw and mulberry leaves fermented by microbial enzymatic hydrolysis as a nitrogen-containing biomass precursor. This invention utilizes renewable bio-based nitrogen-doped carbon to support nickel. The resulting catalyst, when applied to the catalytic reduction separation of straw components, exhibits high reactivity and good stability, enabling efficient catalytic separation and comprehensive utilization of straw components. This invention also provides a method for catalytic separation of straw components, converting lignin in straw into lignin oil while simultaneously separating the solid product holocellulose. Using the method of this invention for catalytic separation of straw components, the lignin oil yield is greater than 30%, and the solid product recovery rate is greater than 85%.

The invention relates to a drug composition for use in the treatment of diarrhea in animals, comprising a microbial enzyme solution, St. John's wort tea, vitamin B1, vitamin B6 and sodium benzoate as a preservative.

This invention relates to the field of agricultural biotechnology and discloses a method for cultivating high-glucan rice based on microbial enzymes. The method includes: fermenting barley flour, mushroom mycelium powder, and other raw materials through aeration to prepare a compound enzyme solution containing a specific activity ratio of β-amylase and glucose polymerase; using the enzyme stock solution prepared in step S1 to treat rice throughout its entire growth cycle from seed to maturity; and using the compound enzyme solution for bioregulation throughout the rice's growth period. Specifically, during the grain-filling stage, the timing of panicle spraying is determined by monitoring the relative chlorophyll content of the rice flag leaf as it transitions from a peak to a stable downward trend. This invention, through precise coupling of the enzymatic reaction system with the rhythm of plant endogenous substance transport, significantly increases the glucan content in the final harvested rice and improves palatability indicators such as chalky grain rate, while maintaining crop yield, without the application of chemically synthesized inputs.

The invention discloses a microbial fermentation solid biological feed and a processing technology thereof, and belongs to the technical field of feeds, the microbial fermentation solid biological feed is obtained by uniformly mixing corn flour, bean flour, a compound bacterium enzyme preparation, table salt, honeysuckle, hawthorn, fishbone dust and deionized water and then granulating; according to the compound bacterium enzyme preparation, sodium lignin sulfonate composite biomass porous carbon serves as a carrier, sodium alginate and calcium ions are subjected to an ionic cross-linking reaction to form gel microspheres, bacillus subtilis powder is wrapped and loaded, and the sodium lignin sulfonate structurally contains reversible quinone and semi-quinone groups and can also be coordinated with sodium alginate; according to the present invention, the composite bacterial enzyme preparation is endowed with certain pH responsiveness, the microcapsule structure is fixed on the sodium lignin sulfonate composite biomass porous carbon, and when the feed enters the intestines and the stomach of the animal, the pH change causes the coordination structure change, such that the microcapsule structure is changed so as to release the microcapsule, such that the slow release effect on the microorganism is achieved, and the precise release is achieved.

The application provides a lysinibacillus sphaericus for producing lipase and application thereof, and relates to the technical field of microbial enzymes. Lysinibacillus sphaericus Yin-202501, and a preservation number of CCTCC No.M2025574.The lysinibacillus sphaericus for producing lipase provided by the application has the following characteristics: Lysinibacillus sphaericus Yin-202501 has the following characteristics: high lipase activity, simple process, and the like. Lysinibacillus sphaericus Yin-202501 is used as an enzyme-producing strain, and olive oil emulsifier and ferrous sulfate are appropriately added in Luria.Bertani (LB) liquid medium, so that the method for preparing a high-activity lipase crude solution by using lysinibacillus sphaericus has the characteristics of simple operation, simple process, good repeatability and the like.

The present application relates to the technical field of feed, and discloses a feed compound microbial enzymolysis method and an enzymolysis device, which comprises the following steps: S1: premixing feed base material crushed to 20-40 mesh and compound enzyme preparation according to a mass ratio of 100:0.3-0.8 to obtain premix; S2: continuously feeding the premix into the enzymolysis device, and performing enzymolysis reaction for 15-30 min under the condition of 35-42 DEG C and a dissolved oxygen value of greater than or equal to 2 mg / L; S3: after the enzymolysis is completed, adding compound microbial liquid into the enzymolysis device on line, adjusting the pH of the system to 5.8-6.2, and continuing to ferment for 2-4 h; and S4: after the fermentation is completed, drying the material at low temperature until the water content is less than or equal to 12%, and obtaining the finished product. By organically combining the enzymolysis and the fermentation process, and performing the enzymolysis and the microbial fermentation in stages under the condition of strictly controlling the temperature, the dissolved oxygen and the pH, the release rate and the bioavailability of the nutritional substances in the feed are significantly improved, and the method has the advantages of short reaction time, high efficiency, strong controllability of conditions, low water content of the finished product and easy storage.