36 results about "Microbial enzymes" patented technology

The present invention relates to functional foods and dietary supplements comprising a specific diamine oxidase (DAO) enzyme derived from Glutamicibacter halophytocola, uses of said enzyme and respective methods for the production of biogenic amine-depleted products, uses of said enzyme as a biosensor for the analysis of one or more biogenic amine(s), as well as said enzyme for use in medicine, in particular for use in the prevention or treatment of a condition or disease that is associated with increased levels of biogenic amines.

This invention relates to the field of low-temperature fermentation formulation technology. The invention provides a low-temperature resistant compound bacterial enzyme formulation, its preparation method, and its application in the co-fermentation of straw and manure. The compound bacterial enzyme formulation comprises the following raw materials in parts by weight: 8-10 parts of compound bacterial agent, 1-1.4 parts of compound enzyme agent, and 88.6-91 parts of excipients. The compound bacterial enzyme formulation of this invention can ferment straw and manure under conventional conditions as well as at low temperatures of around 15°C, effectively meeting the fermentation requirements in low-temperature regions. By combining the excipients with the bacteria and enzymes, substances and nutrients during the fermentation process can be effectively adsorbed, avoiding acid inhibition, and providing attachment sites for the bacteria and enzymes, thus prolonging their activity.

The application relates to a crisp type pre-conditioned food of tussah pupae and a preparation method thereof. The method is characterized in that fresh tussah pupae are used as raw materials, a series of processing technologies such as spraying and moisturizing of organic acid, temperature control, inorganic acid immersion and killing, microbial-enzymatic treatment of tussah pupae, pickling and baking are combined to prepare the crisp type pre-conditioned food of tussah pupae, the method is simple in operation, can change the permeability of the chitin shell of the tussah pupae, increase the migration rate of the seasoning, reduce the proportion of the burst plasma, and simultaneously reduce the oil oxidation caused by repeated baking treatment.

The application provides a low-cost artificial feed for domestic silkworms and a preparation method thereof, and relates to the technical field of domestic silkworm breeding.The low-cost artificial feed for domestic silkworms is composed of mulberry leaf powder, corn powder, organic acid, sitosterol, microorganisms, enzyme preparation, vitamins, minerals and the like, and can effectively improve the stress resistance of the domestic silkworms, and improve the silk production rate of the silkworms and the quality of the silk.

The invention provides a fermented corncob as well as a preparation method and application thereof, and belongs to the technical field of ruminant nutrition and feed science. The fermented corncob is prepared from the following raw material components in parts by mass: 32 to 42 parts of corncob, 2.5 to 5 parts of soybean meal, 3 to 5 parts of cottonseed meal, 2 to 4 parts of wheat bran, 2 to 5 parts of rice bran, 2 to 5 parts of glucose, 2.5 to 5 parts of cane molasses, 0.2 to 0.5 part of expanded urea, 2 to 4 parts of attapulgite powder, 0.4 to 0.6 part of compound bacterium enzyme mixture, 0.3 to 0.5 part of feed-grade sodium chloride, 0.3 to 0.5 part of feed-grade magnesium oxide and 34 to 42 parts of water. The corncob and other raw materials are subjected to mixed fermentation through combination of various bacteria and enzymes, so that the nutritional value and the nutritional level of the feed are improved. The fermented corncob can replace part of corn in the feed, the production performance is improved, the feed cost is reduced, resource recycling is effectively promoted, and the palatability of the feed is improved.

The invention discloses a microbial enzyme synergistic pymetrozine and nitenpyram water dispersible granule which comprises the following components in percentage by weight: 40-70% of a pymetrozine active compound, 10-25% of a nitenpyram active compound, 1-10% of microbial enzyme, 1-15% of a dispersing agent, 1-10% of a wetting agent, 1-10% of a stabilizer, 1-10% of a buffer solution, 2-10% of an adhesive and the balance of a carrier. The invention also provides a preparation method of the composition. Compared with the prior art, the synergistic water dispersible granule has the advantages that the epidermis or digestive system of pests is destroyed by enzyme generated by specific microorganisms, and the pymetrozine and nitenpyram are combined to form the synergistic water dispersible granule, so that the control effect can be improved, the use of chemical agents can be reduced, and the water dispersible granule is short in disintegration time and low in cost. The suspension rate is greater than or equal to 90%, and the suspension can be used for unmanned aerial vehicle flight prevention and adapts to different water quality conditions.

The invention relates to an automatic multi-temperature-control microplate reader based on integration of a mechanical arm and artificial intelligence (AI), and aims to improve automation and accuracy of microbe growth monitoring and analysis. Operation such as culture dish taking and placing, culture solution adding and timing sampling can be automatically completed through the mechanical arm. The AI algorithm automatically adjusts parameters such as culture temperature, humidity, illumination and the like according to real-time data and environmental conditions so as to optimize the growth environment of microorganisms. Meanwhile, key indexes such as a light absorption value and cell density are monitored, a microorganism growth curve is drawn in combination with AI analysis, and important parameters such as the growth rate and the maximum growth amount are calculated. The AI can also mine potential laws in the data and provide valuable research information. The instrument improves the efficiency, accuracy and reliability of microbiological research, is widely applied to the fields of microbiological growth law research, resource development, disease diagnosis and the like, and promotes related technical progress.

The invention relates to a liquid microbial enzyme as well as a preparation method and application thereof. The liquid microbial enzyme is prepared by taking soybean meal, fish meal, rice husk and water as raw materials, sterilizing and fermenting through a compound microbial agent, and the compound microbial agent comprises bacillus subtilis, bacillus licheniformis and bacillus velezensis. In the application, the soybean meal serves as a core nitrogen source and an energy source, soybean isoflavone of the soybean meal promotes functional bacteria to proliferate and enhances enzyme activity, and a fiber structure can optimize fermentation conditions; the fish meal provides high-quality protein which can be decomposed to generate organic acid, so that the pH value is reduced, and putrefying bacteria are inhibited; the rice husks maintain the material structure, and the porous characteristic improves ventilation and adsorbs peculiar smell; the bacillus velezensis as an auxiliary enzyme source can enhance the variety coverage of soluble enzymes, so that the substrate degradation capacity is more comprehensive. After the liquid microbial enzyme is prepared, the liquid microbial enzyme and the hermetia illucens are combined, livestock and poultry manure is used as a conversion object, and the conversion efficiency of the livestock and poultry manure on the manure is improved by regulating and controlling intestinal microflora of larvae of the hermetia illucens.

The invention provides a microbial enzyme catalytic synthesis method of 20 alpha-hydroxyprogesterone, and belongs to the technical field of biosynthesis. The invention establishes an efficient, green and high-selectivity 20 alpha-hydroxyprogesterone biosynthesis method, and the method has the direct effects that the directional hydroxylation of the C20 site of the substrate progesterone is realized by utilizing a recombinase technology, the generation of byproducts is effectively avoided, the subsequent separation and purification process is simplified, the reaction conditions are mild, the cost is low, and the method is suitable for industrial production. The energy consumption and the safety risk are reduced; from the aspect of product application significance, the method provides a stable and reliable material source for large-scale preparation of 20 alpha-hydroxyprogesterone, the environmental protection property and economical efficiency of the production process are remarkably improved, a solid material foundation is laid for in-depth study on the biological activity and application of the compound, and the method is suitable for industrial production. And a beneficial technical reference is provided for green biological manufacturing of other steroid hormone medicines.

The invention relates to the technical field of organic fertilizer production and processing, in particular to a microbial enzymolysis device for organic fertilizer production, which comprises a heating tank, a timing discharging mechanism and a stirring mechanism are arranged on the heating tank, the timing discharging mechanism comprises a plurality of storage tanks, each storage tank is in threaded connection with a cover, and the cover is in threaded connection with the stirring mechanism. Lifting rods are slidably connected to the bottom walls of the storage tanks, linkage balls are fixedly connected to the bottom ends of the lifting rods, lifting plates are fixedly connected to the top ends of the lifting rods, springs are fixedly connected to the bottom ends of the lifting plates, and a plurality of sealing rods arranged in an array mode are fixedly connected to the outer walls of the lifting plates. The method disclosed by the invention has the beneficial effects that the high-solid-content and high-viscosity organic substrate has no standing dead zone and is in full contact with enzyme liquid and microorganisms, and the enzymatic hydrolysate can be uniformly dispersed along with the reaction process, so that not only is the insufficient enzyme amount in a substrate aggregation region avoided, but also the excessive accumulation of the enzyme liquid in a substrate sparse region is prevented, and the stability of the enzymatic hydrolysis reaction is ensured.

This invention relates to a phthalate hydrolase mutant and its application in phthalate degradation, belonging to the field of microbial enzyme technology. The amino acid sequence of the phthalate hydrolase mutant HylD1 is shown in SEQ ID NO.1, and the nucleotide sequence of the encoding gene is shown in SEQ ID NO.2. This enzyme can catalyze the reaction to generate MMP using DMP as a substrate; or catalyze the reaction to generate MEP and EMP using DEP as a substrate. In this invention, the encoding gene of the phthalate hydrolase HylD1 from *Paracoccus koningii* BJQ0001 was artificially modified by site-directed mutagenesis at amino acid position 265, resulting in a mutant HylD1 (R265A) with higher activity. Compared with the wild-type phthalate hydrolase HylD1, the absolute enzyme activity of this phthalate hydrolase mutant HylD1 (R265A) in degrading DMP and DEP is increased by 2-fold and 3.6-fold, respectively, and a special product EMP is also produced during the degradation of DEP.

This invention discloses a *Aspergillus spp.* SLP7-15 and its application in the extraction of polyphenols from pomegranate peel. The fermentation broth obtained from *Aspergillus spp.* SLP7-15 culture is filtered, and the filtrate is mixed with pomegranate peel powder. Enzymatic hydrolysis is performed at 35–40℃ for 4–6 hours, followed by the addition of deionized water and anhydrous ethanol. The mixture is then subjected to ultrasonic extraction, filtered, evaporated under reduced pressure, dissolved in anhydrous ethanol, and vacuum dried to obtain the polyphenol extract. Before the ultrasonic extraction of pomegranate peel polyphenols with ethanol, the addition of crude enzyme solution prepared from *Aspergillus spp.* SLP7-15 fermentation facilitates the dissolution of polyphenols, significantly improving the polyphenol extraction yield. The microbial enzymatic hydrolysis-assisted extraction method for pomegranate peel polyphenols provided by this invention can increase the polyphenol extraction yield by 26.6% compared to the direct ultrasonic extraction method with ethanol.

This invention provides a supported nickel-based catalyst, its preparation method, and a method for catalytic separation of straw components, relating to the field of catalytic materials technology. The supported nickel-based catalyst provided by this invention comprises a bio-based nitrogen-doped carbon support and active metallic nickel supported on the bio-based nitrogen-doped carbon support. The bio-based nitrogen-doped carbon support is obtained by pyrolysis and carbonization of a mixture of straw and mulberry leaves fermented by microbial enzymatic hydrolysis as a nitrogen-containing biomass precursor. This invention utilizes renewable bio-based nitrogen-doped carbon to support nickel. The resulting catalyst, when applied to the catalytic reduction separation of straw components, exhibits high reactivity and good stability, enabling efficient catalytic separation and comprehensive utilization of straw components. This invention also provides a method for catalytic separation of straw components, converting lignin in straw into lignin oil while simultaneously separating the solid product holocellulose. Using the method of this invention for catalytic separation of straw components, the lignin oil yield is greater than 30%, and the solid product recovery rate is greater than 85%.

The invention relates to a drug composition for use in the treatment of diarrhea in animals, comprising a microbial enzyme solution, St. John's wort tea, vitamin B1, vitamin B6 and sodium benzoate as a preservative.

This invention relates to the field of agricultural biotechnology and discloses a method for cultivating high-glucan rice based on microbial enzymes. The method includes: fermenting barley flour, mushroom mycelium powder, and other raw materials through aeration to prepare a compound enzyme solution containing a specific activity ratio of β-amylase and glucose polymerase; using the enzyme stock solution prepared in step S1 to treat rice throughout its entire growth cycle from seed to maturity; and using the compound enzyme solution for bioregulation throughout the rice's growth period. Specifically, during the grain-filling stage, the timing of panicle spraying is determined by monitoring the relative chlorophyll content of the rice flag leaf as it transitions from a peak to a stable downward trend. This invention, through precise coupling of the enzymatic reaction system with the rhythm of plant endogenous substance transport, significantly increases the glucan content in the final harvested rice and improves palatability indicators such as chalky grain rate, while maintaining crop yield, without the application of chemically synthesized inputs.

The invention discloses a microbial fermentation solid biological feed and a processing technology thereof, and belongs to the technical field of feeds, the microbial fermentation solid biological feed is obtained by uniformly mixing corn flour, bean flour, a compound bacterium enzyme preparation, table salt, honeysuckle, hawthorn, fishbone dust and deionized water and then granulating; according to the compound bacterium enzyme preparation, sodium lignin sulfonate composite biomass porous carbon serves as a carrier, sodium alginate and calcium ions are subjected to an ionic cross-linking reaction to form gel microspheres, bacillus subtilis powder is wrapped and loaded, and the sodium lignin sulfonate structurally contains reversible quinone and semi-quinone groups and can also be coordinated with sodium alginate; according to the present invention, the composite bacterial enzyme preparation is endowed with certain pH responsiveness, the microcapsule structure is fixed on the sodium lignin sulfonate composite biomass porous carbon, and when the feed enters the intestines and the stomach of the animal, the pH change causes the coordination structure change, such that the microcapsule structure is changed so as to release the microcapsule, such that the slow release effect on the microorganism is achieved, and the precise release is achieved.

The application provides a lysinibacillus sphaericus for producing lipase and application thereof, and relates to the technical field of microbial enzymes. Lysinibacillus sphaericus Yin-202501, and a preservation number of CCTCC No.M2025574.The lysinibacillus sphaericus for producing lipase provided by the application has the following characteristics: Lysinibacillus sphaericus Yin-202501 has the following characteristics: high lipase activity, simple process, and the like. Lysinibacillus sphaericus Yin-202501 is used as an enzyme-producing strain, and olive oil emulsifier and ferrous sulfate are appropriately added in Luria.Bertani (LB) liquid medium, so that the method for preparing a high-activity lipase crude solution by using lysinibacillus sphaericus has the characteristics of simple operation, simple process, good repeatability and the like.

The invention provides a method for pickling pseudosciaena crocea in a low-sodium manner through lysine under the assistance of vacuum and ultrasonic assistance, and belongs to the technical field of aquatic product processing. The method for low-sodium pickling of the large yellow croaker with lysine under the assistance of vacuum and ultrasound comprises the following steps: (1) pretreating the large yellow croaker, and preparing a low-sodium pickling agent; (2) soaking the pretreated pseudosciaena crocea in the low-sodium pickling agent, and sequentially performing vacuum-assisted ultrasonic-assisted pickling and static pickling; and (3) drying and dehydrating the pseudosciaena crocea pickled in the step (2) to obtain a finished product. Larimichthys crocea is pickled with lysine in a low-sodium manner under the assistance of vacuum and ultrasonic assistance, the pickling efficiency is improved while the use amount of sodium salt is reduced, and the defects that common low-sodium pickling is low in efficiency and poor in flavor are overcome; fat oxidation caused by microorganisms, enzymes, free radicals and the like in the pickling period is effectively reduced, and the storage stability of the product is improved.

The invention discloses a water quality purifying agent based on microalgae fermentation. The water quality purifying agent is prepared from alkaline protease, cellulase, a spirulina extract, bacillus subtilis, geobacillus, bacillus amyloliquefaciens, thermophilic bacteria, halotolerant bacteria, nano calcium carbonate, chitosan and spirulina micro powder. Microalgae, microorganisms and an enzyme preparation are adopted to form a synergistic system, the purification effect stability is improved, the efficient and long-acting water quality purification requirements in an extreme environment are met, and the water quality purifying agent has dynamic adaptability, synergistic interaction and environmental friendliness. The preparation method comprises the following steps: directional pretreatment of spirulina, construction of a dynamic flora proportion regulation and control system, culture of complex functional flora, preparation of a nano composite carrier, preparation of a bacterium-algae enzyme complexing agent and the like.

This invention discloses a liquid fertilizer containing small-molecule organic matter and its preparation method, comprising the following components by weight: 30-50 parts humic acid, 15-25 parts fulvic acid, 20-40 parts free amino acids, 20-40 parts urea, 15-30 parts ammonium dihydrogen phosphate, 15-35 parts potassium sulfate, 5-15 parts EDTA-chelated trace elements, 2-8 parts composite stabilizer, and 150-250 parts water; the mass ratio of fulvic acid to humic acid is 1:1.5-2.5; the free amino acids... The mass ratio of amino acids to fulvic acid is 1:0.5-1.2; the proportion of organic matter with a molecular weight less than 1000 Da in the liquid fertilizer is ≥65%; macromolecular organic matter is efficiently converted into small molecular form through microbial enzymatic decoupling process; humic acid, fulvic acid and free amino acids are used to give full play to the synergistic effect of multiple organic components; through multi-stage synergistic chelation process, the chelation rate of trace elements can reach more than 90%; the product does not separate after being stored at room temperature for more than 12 months.

The application discloses a time sequence control type composite pollution repair material for facility agriculture soil and a preparation method and application thereof, and belongs to the technical field of soil repair. The repair material is a three-layer core-shell structure particle, which comprises, from inside to outside, an inner core layer composed of biodegradable polyester wrapping pesticide degrading enzyme and pesticide adsorbent, a middle layer composed of water / ion responsive hydrogel wrapping heavy metal fixing agent and oxidative degradation catalyst, and an outer shell layer composed of brittle degradable polymer wrapping micro / nano plastic surface capping agent. The repair material realizes time sequence and synergistic repair of micro / nano plastic, available Cd and malathion by triggering the release of the outer shell layer through plowing mechanical force, triggering the slow release of the middle layer through irrigation water response, and triggering the release of the inner core layer through rhizosphere microbial enzyme. The material has simple preparation process, convenient application and good application prospect.

The invention provides lysinibacillus for producing lipase and application of the lysinibacillus, and relates to the technical field of microbial enzymology. The lysinibacillus subtilis for producing the lipase is named as Lysinibacillus capsici Yin-202501, and the preservation number of the lysinibacillus capsici Yin-202501 is CCTCC (China Center for Type Culture Collection) No. M2025574. The Lysinibacillus capsici Yin-202501 disclosed by the invention has the following characteristics that the activity of producing the lipase is high, and the process is simple. According to the method, Lysinibacillus capsici Yin-202501 is used as an enzyme producing strain, a proper amount of olive oil emulsifier and ferrous sulfate are added into a Luria. Bertani (LB) liquid culture medium, the method for preparing the high-activity lipase crude solution from the lysinibacillus has the advantages of being easy to operate, simple in process, good in repeatability and the like, the prepared lipase solution is high in activity, the yield is high, and the method is suitable for industrial production. The method has good application potential and development and utilization prospects in the industrial field.

The invention discloses fermented black tea with functions of resisting oxidation and reducing blood sugar as well as a preparation method and application of the fermented black tea. The fermented black tea is prepared from the following raw materials in parts by mass: 100 parts of fresh tea leaves and 1.5-5.5 parts of a composite strain preparation, wherein the composite strain preparation is prepared from lactobacillus rhamnosus LR-C55 and lactobacillus plantarum H-87, and the mass ratio of the lactobacillus rhamnosus LR-C55 to the lactobacillus plantarum H-87 is (1 to 1) to (1 to 3). The preparation method comprises the following steps: selecting fresh tea leaves, and performing withering treatment at room temperature to obtain withered tea leaves; performing surface sterilization on the withered tea leaves through ultraviolet irradiation; mixing the composite strain preparation with deionized water, and activating to obtain a bacterial liquid; putting the sterilized withered tea leaves into a fermentation container, and spraying bacterial liquid for fermentation to obtain fermented tea leaves; and step 6, putting the fermented tea leaves into drying equipment, and carrying out sectional drying, so as to obtain the fermented black tea. The tea polyphenol is directionally converted through a microbial enzyme system, so that the antioxidant activity of the fermented black tea and the function of assisting in regulating postprandial blood sugar are remarkably improved.

The application provides a floor oil sludge microbial treatment inducer and application thereof. The floor oil sludge microbial treatment inducer comprises a modified pretreatment agent and an exogenous microbial enzyme activity promoter; the modified pretreatment agent comprises sodium carboxymethyl cellulose, sodium carboxyethylidene diphosphonate, sodium humate, kaolinite and ethanol; and the exogenous microbial enzyme activity promoter comprises a KH2PO4-K2HPO4 buffer, yeast powder, lactose, linseed oil and petroleum ether. The floor oil sludge microbial treatment inducer can solve the problem of low efficiency of microbial treatment of floor oil sludge soil in the prior art and is suitable for the field of oil pollution treatment.

The invention discloses a compound bacterium enzyme preparation containing alpha-L-arabinofuranosidase AraA02173 and application of the compound bacterium enzyme preparation in fermentation of air-dried highland barley vinasse, and belongs to the technical field of microbial enzyme preparations. The invention provides an amino acid sequence of the alpha-L-arabinofuranosidase AraA02173 and a nucleotide sequence of a coding gene of the alpha-L-arabinofuranosidase AraA02173, and the alpha-L-arabinofuranosidase AraA02173 can efficiently degrade glycoside substances. The enzyme AraA02173 and the xylanase are combined and cooperate with the compound microbial agent to ferment the highland barley vinasse, the enzyme AraA02173 can remarkably improve the lactic acid value and the reducing sugar content of the highland barley vinasse, the content of neutral detergent fibers and the content of acid detergent fibers are reduced, the feeding nutritional value of the fermented highland barley vinasse is increased, and the yield of the highland barley vinasse is increased. The fermented highland barley vinasse is a high-quality feed raw material which is high in nutritional value, good in palatability and easy to store, has a wide application prospect in animal husbandry, and is of great significance to large-scale production of high-quality fermentation of industrial highland barley vinasse.

The invention discloses a deodorant and a preparation method thereof. The deodorant comprises the following components in parts by weight: 10%-20% of photosynthetic bacteria, 5%-15% of streptococcus lactis, 5%-15% of bacillus amyloliquefaciens, 10%-20% of proteolytic enzyme, 15%-25% of compound enzyme, 10%-20% of tartaric acid and 5%-15% of free fatty acid. By adopting the synergistic effect of various microorganisms, enzymes and chemical components, the deodorant is efficient in deodorization and can quickly remove various stink; environment-friendly and safe components are selected, pollution to the environment is avoided, harmful residues are avoided, and the water purifying agent can be safely used for various water bodies and does not harm aquatic organisms and human health; the water quality improvement function is achieved, organic pollutants can be decomposed, nutrients are balanced, and the water quality is improved; the formula is high in adaptability, has a good effect on different types of odor sources, and is wide in application range; the preparation process is scientific and reasonable, operation steps and parameters of all links are clear, stable and reliable quality is guaranteed, and large-scale production and popularization are facilitated.

The invention discloses a compound bacterium enzyme preparation containing acetylxylan esterase AxeA16138 and application of the compound bacterium enzyme preparation in fermentation of highland barley straw, and belongs to the technical field of microbial enzyme preparations. The invention provides an amino acid sequence of acetyl xylan esterase AxeA16138 and a nucleotide sequence of an encoding gene of the acetyl xylan esterase AxeA16138, CBM1 members in the acetyl xylan esterase AxeA16138 have adhesion ability to insoluble microcrystalline cellulose and wheat arabinoxylan, and the acetyl xylan esterase AxeA16138 can efficiently degrade ester substances. According to the method, enzyme AxeA16138 and xylanase are combined and cooperate with a compound microbial agent to ferment the highland barley straw, the enzyme AxeA16138 effectively degrades fiber components of the highland barley straw, the content of crude protein and crude fat is remarkably increased, the nutritional quality and feeding value of the highland barley straw are improved, and the application value of the enzyme AxeA16138 in animal husbandry is further widened. The method has important significance on large-scale production of high-quality fermentation of industrial highland barley straws.