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34 results about "Marine biology" patented technology
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Marine biology is the scientific study of marine life, organisms in the sea. Given that in biology many phyla, families and genera have some species that live in the sea and others that live on land, marine biology classifies species based on the environment rather than on taxonomy.
Seaweed product for agriculture and preparation method of fucoidin
InactiveCN1919876AMaintain biological activityImprove qualityBiocideAnimal repellantsBiotechnologyFucus evanescens
The invention discloses a comprehensive utilization usage of seaweed; especially production method of involving producing seaweed agricultural product and fucosido- polysaccharide; which comprise the following parts: adopting marine biology sodium as main material; applying freezing disintegrating technology, ultrasonic wave water and lixivium extraction steps; extracting inherent autogenous plant growing modifier in maximum limit and keeping biological activity; separating fucosido-polysaccharide and producing bottom of last leaving to fertilizer, soil modifier and fodder; that can be using fully.
Owner:YANTAI DIYUAN BIOTECH
Shark protein antihypertensive peptide and preparation method and application thereof
InactiveCN101240016AHigh ACE inhibitory activityGood ACE inhibitory activityPeptide preparation methodsDipeptidesMicrobial enzymesProteolysis
Shark protein antigypertensive peptide, preparation and application thereof, which pertains to marine biology technical field. The novel antigypertensive peptide with two amino acid separated and purified from proteolysis product of shark, has a high inhibiting activity for angiotensin converting enzyme (ACE). Preparation includes steps of carrying out enzymolysis for shark protein to prepare enzymatic hydrolysate, seperating and purifying peptide from the enzymatic hydrolysate, and sequencing of peptide. The invention is applicable for preparing antihypertensive medicament. The invention apply microbial enzyme technology to deep development of shark, obtain peptide having novel sequence, with high inhibiting activity for ACE.
Owner:SHANDONG UNIV
Cyanea capillata polypeptide growth factor, preparation method and applications thereof
ActiveCN103819552ASignificant proliferative effectPeptide/protein ingredientsPeptide preparation methodsDamage repairOn cells
The invention belongs to the technical field of marine biology, and provides a cyanea capillata polypeptide growth factor named as Cc-GRN-1. The amino sequence of the growth factor is represented by the SEQ ID No. 1. The invention also provides a preparation method and applications of the cyanea capillata polypeptide growth factor. The cyanea capillata polypeptide growth factor has a prominent promoting effect on cell proliferation, and has a good application prospect in development of damage repairing drugs.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Biomimetic manta ray device
PendingCN107323638AReduce noiseRealize fully flexible anti-biological movementUnderwater equipmentTransceiverAcoustic wave
The invention relates to a biomimetic manta ray device. The biomimetic manta ray device comprises a manta ray body, a pectoral fin body, a multiplex steering engine control plate and a main control single chip microcomputer. The manta ray body is fixedly provided with an acoustic wave sensor, a magnetic field sensor, an acceleration sensor, a temperature sensor, a pressure sensor and a multi-channel wireless communication transceiver device. The front end of the manta ray body is provided with a high definition micro camera. The signal output ends of an infrared obstacle avoidance instrument, a water pressure sensor, the temperature sensor, the acoustic wave sensor, the acceleration sensor and the magnetic field sensor are correspondingly and electrically connected with each signal input end of the main control single chip microcomputer. The biomimetic manta ray device has the advantages that fully compliant biomimetic motion can be achieved, resistance is low, speed is quick and flexible, accelerate starting can be achieved, timely feedback of data of underwater communication is facilitated, noise is reduced to a great extent during the biomimetic manta ray device swimming through dynamic mechanism of gliding, concealment is achieved advantageously, and the good application prospect in marine biology research and sea bottom survey is achieved.
Owner:SHANGHAI OCEAN UNIV
Process for improving nutritional value of puffer fish skin
InactiveCN110495579AHigh nutritional valueImprove antioxidant capacityMicroorganism based processesFermentationNutritional statusSeparation technology
The invention discloses a process for improving a nutritional value of puffer fish skin, and belongs to the technical field of marine biology. The process includes the following steps that the pufferfish skin is taken as raw materials, a modern biological separation technology, a biological enzymolysis technology and a mixed bacteria microbial fermentation technology are integrated to prepare anddevelop puffer fish skin polypeptide biological products with high in-vitro antioxidant capacities, mixed bacteria aerobic fermentation strains mainly include candida utilis, bacillus subtilis, and bacillus coagulans. According to the process, obtained puffer fish skin polypeptides have the strong antioxidant capacity, an extracting solution is subjected to concentration, absorption and drying, alcohol-precipitation or resin purification and other treatments according to application ways, so as to meet efficient utilization in health care, food, medicine and other fields; and the process technology is simple and efficient, obtained products have the strong antioxidant capacity and the free radical scavenging capacity, product stability is strong, the production cost is low, and industrialproduction and large-scale promotion are easily conducted.
Owner:青岛浩大生物科技工程有限责任公司
Chinese white dolphin dorsal fin identification method based on convolutional neural network
PendingCN110837818AImprove efficiencyImprove positioning accuracyBiometric pattern recognitionImaging qualityTest sample
The invention discloses a Chinese white dolphin dorsal fin identification method based on a convolutional neural network, and the method comprises the steps: constructing a Chinese white dolphin dorsal fin image library with a position box and an identification tag, and dividing the Chinese white dolphin dorsal fin image library into a training sample and a test sample; training a convolutional neural network for positioning a dorsal fin region; respectively training three convolutional neural networks for identifying the left and right sides of the dorsal fin, the image quality of the dorsalfin and an individual to which the dorsal fin belongs; positioning a dorsal fin region of the image in the test sample through a convolutional neural network for positioning; and identifying the dorsal fin area through the three convolutional neural networks for identification, and outputting identification results of the left and right sides of the dorsal fin, the dorsal fin image quality and theindividual to which the dorsal fin belongs. According to the method, the feature learning capability of the convolutional neural network is fully utilized, automatic identification of the dorsal finof the Chinese white dolphin can be efficiently and accurately realized, and marine biologists are helped to analyze the individual habits and population characteristics of the Chinese white dolphin.
Owner:SHANTOU UNIV
Spleen-benefiting and stomach-strengthening euphausia superba oil polypeptide biological product
InactiveCN110663803AImprove antioxidant capacityHealth benefits of strengthening the spleen and strengthening the stomachProtein composition from fishHydrolysed protein ingredientsOil processingBacillus cereus
The present invention discloses a spleen-benefiting and stomach-strengthening euphausia superba oil polypeptide biological product and a preparation technology thereof, and belongs to the technical field of marine biology. Euphausia superba oil processing waste is used as a raw material, a modern biological separation technology, a biological enzymolysis technology and a mixed micro-organism fermentation technology are integrated, and the euphausia superba oil polypeptide biological product with significant spleen-benefiting and stomach-strengthening effects is developed, wherein mixed micro-organism aerobic fermentation strains mainly include aspergillus niger, bacillus subtilis and bacillus cereus. The obtained euphausia superba oil polypeptide has relatively strong spleen-benefiting andstomach-strengthening health-care effects. An extract can be subjected to concentration, adsorption and drying, alcohol-precipitation, or purification by resin, etc. according to application routes so as to meet efficient use in the fields of health care, food, medicines, etc. The technology is simple and efficient, and the obtained product has strong anti-oxidation capacity and spleen-benefitingand stomach-strengthening health-care effects, and is strong in product stability, low in production cost and easy for industrial scale production.
Owner:付鹏磊
A multi-source information fusion algorithm for marine biology detection and early warning in nuclear power plant
ActiveCN109190718AImprove reliabilityBiological neural network modelsCharacter and pattern recognitionAlgorithmNuclear power
The invention relates to a multi-source information fusion algorithm for marine biology detection and early warning in a nuclear power plant. For the data collected by many sensors in the field, the algorithm adopts decision-making fusion form, analyzes and calculates the data respectively based on linear regression model and fuzzy neural network prediction method, and obtains the early warning model of multi-source information fusion for marine biology exploration and early warning. The intrusion intensity formula, fitting error and Euclidean distance are obtained by using the least square multiparameter estimation method of a linear multiple regression model estimation method and the linear regression function of MATLAB. The fuzzy theory and neural network technology are used to fit thesensor sampling data, and a non-parametric multi-sensor data fusion algorithm is implemented to determine the invasion degree of marine organisms and the possibility of water intake blockage, which provides support for improving the reliability of nuclear power plant cold source.
Owner:SUZHOU NUCLEAR POWER RES INST +2
Method for detecting whistle signal of cetacean animal based on Mel subband spectral entropy
The invention provides a method for detecting a whistle signal of a cetacean animal based on Mel subband spectral entropy. From the perspective of spectral entropy, combining with a Mel filter, dolphin whistle signals are detected by calculating the Mel subband spectral entropy, and the false alarm probability is further reduced by the duration threshold to improve the detection accuracy. The method is simple and easy, and can realize automatic detection and extraction of whistle signals, thereby reducing the workload of artificial extraction. The dolphin whistle signals have important value for research on bionic camouflage underwater acoustic communication, dolphin population classification and recognition and the like, and therefore, the method can assist in bionic camouflage underwateracoustic communication, marine biology and other related research.
Owner:HARBIN ENG UNIV
Gene editing method for electrotransfected bivalve mollusks
PendingCN114164232AEasy to operateSimple equipment requirementsHydrolasesInvertebrate cellsBivalve shellZoology
The invention belongs to the field of marine biology application, particularly relates to the field of shellfish molecular genetic breeding, and relates to an electrotransfection bivalve shellfish gene editing method, the concentration of recombinant plasmids is kept to be greater than or equal to 2 mu g / mu l, sgRNA and Cas9 protein are mixed into RNP (sgRNA and Cas9 protein complex) according to the volume ratio of 2: 1, the working concentration of the sgRNA is greater than or equal to 50 ng / mu l, the working concentration of the Cas9 is greater than or equal to 250 ng / mu l, the working concentration of the sgRNA is greater than or equal to 50 ng / mu l, and the working concentration of the Cas9 is greater than or equal to 250 ng / mu l; an electrotransfection method is applied to bivalve mollusk gene editing, the electric pulse condition is that the pulse interval is 1s, the number of pulses is 2, the electrode distance is 4mm, pulse voltage and pulse time are changed, the pulse voltage range is 50V-500V, and the pulse time is 100 microseconds-1ms. The method disclosed by the invention can provide a basis for bivalve gene function verification and gene breeding application, and has the advantages of simplicity in operation, low cost, easiness in popularization and the like.
Owner:SHANTOU UNIV
Electrochemical reactor for preparing oligosaccharide from marine biology functional sugars and preparation method
ActiveCN104233365AMaximize efficiencyImprove efficiencyElectrolysis componentsElectrolytic organic productionElectrochemical responseElectrolysis
The invention provides an electrochemical reactor for preparing oligosaccharide from marine biology functional sugars and a preparation method and belongs to the electrolysis field. The technical problem that the oligosaccharide cannot be prepared from the marine biology functional sugars through an electrochemical reaction in the prior art is solved. The electrochemical reactor comprises a reaction tank which comprises an empty chamber; two separating plates are fixed in the reaction tank; the empty chamber is divided into three chambers through the two separating plates; the three chambers comprise a reaction chamber and two flow chambers which are located on the bilateral sides of the reaction chamber respectively; each separating plate is provided with a circulation hole which is communicated with the reaction chamber and the corresponding flow chamber; a polar plate is arranged in the reaction chamber. According to the electrochemical reactor, the integration is easy to control and optimize. The preparation method for preparing the oligosaccharide from the marine biology functional sugars comprises step A, feeding; step B, reaction; step C, discharging; step D, separation and collection. According to the preparation method, the oligosaccharide can be extracted from the marine biology functional sugars.
Owner:ZHEJIANG CHENGXIN PHARMA & CHEM EQUIP
A marine life model display device
ActiveCN113163962BChange the direction of the swipeEasy to observeShow cabinetsHousehold cleanersDisplay deviceEngineering
The invention belongs to the technical field of marine life, in particular to a display device for a model of marine life. Aiming at the troublesome problem that the existing display device for a model of marine life will adhere to a lot of dust after being placed for a period of time, and requires regular cleaning by staff, The following proposal is proposed, which includes a booth base, a card slot is opened on the top of the booth base, a turntable is rotatably connected in the card slot, a display cabinet is fixedly connected to the top of the turntable, and a top plate is rotatably connected to the top plate of the booth. Four screw rods are rotatably connected between the bases, the distances between the four screw rods are the same, and the rotation of the two screw rods on the symmetrical axis of the same booth base is consistent, and sliders are set on the four screw rods. The present invention Through the cooperation of the corresponding two half-thread grooves and the screw rod, the slider and the annular plate can be pushed to slide on the screw rod, and then the surface of the display cabinet can be cleaned by the brush on the annular plate, which is convenient and effective.
Owner:TANGSHAN HARBIN SHIP TECH CO LTD +1
Composition for inhibiting skin decomposition of stichopus japonicus and application
The invention relates to a composition for inhibiting skin degradation of stichopus japonicus and application, and belongs to the technical field of marine biology. The composition is prepared from, by final concentration, 30 [mu] M of Emrican, 20 [mu] M of Ilomastat and 15 [mu] g / ml of Nafamostat Mesylate, the volume ratio of DMSO is 1%, and a solvent is ultrafiltration seawater. Fresh and live sea cucumbers are soaked in a solution composed of the composition at 4 DEG C for 45 min. The soaked fresh and live sea cucumbers can be placed on ice to be preserved for 96 hours without autolysis after being irradiated by a UVA lamp with the radiation intensity being 15 W / m<2> and the wavelength being 365 nm for 40 minutes.
Owner:DALIAN OCEAN UNIV
A multi-source information fusion method for detection and early warning of marine organisms in nuclear power plants
ActiveCN109190718BImprove reliabilityBiological neural network modelsCharacter and pattern recognitionAlgorithmEarly warning model
The invention relates to a multi-source information fusion algorithm for detection and early warning of marine organisms in a nuclear power plant. It is mainly aimed at the data collected by multiple sensors on the site, adopts a decision-level fusion form, and performs data respectively based on a linear regression model and a fuzzy neural network prediction method. Through analysis and calculation, the early warning model of multi-source information fusion for marine life detection and early warning is obtained; it uses the least squares multi-parameter function estimation method in the linear multiple regression model estimation method, and combines the linear regression function that comes with MATLAB to perform multi-parameter estimation Obtain the calculation formula of intrusion intensity, fitting error and Euclidean distance, etc.; use fuzzy theory and neural network technology to fit sensor sampling data, and realize non-parametric multi-sensor data fusion algorithm to determine the degree of marine biological invasion and the occurrence of water intake The possibility of blockage, etc., provides support for the overall improvement of the reliability of the cold source of the nuclear power plant.
Owner:SUZHOU NUCLEAR POWER RES INST +2
Marine biology anatomy experiment table
InactiveCN112916077AEasy to clamp and fixEasy to useLaboratory benches/tablesElectric machineryEngineering
The invention discloses a marine biology anatomy experiment table, which comprises a workbench, an anatomy groove is formed in the upper end face of the workbench, and a fixing assembly is arranged in the anatomy groove; the fixing assembly comprises a rotating screw rod, a rotating motor, a fixing clamp, a first sliding block and a second sliding block; the length direction of the rotating screw rod is consistent with the length direction of the anatomy groove, one end of the rotating screw rod is rotatably connected with the side face of one end of the anatomy groove, the other end of the rotating screw rod is in transmission connection with a rotating motor arranged on the side face of the workbench, one end of the rotating screw rod is provided with a first external thread, the other end of the rotating screw rod is provided with a second external thread, and the first external thread and the second external thread are opposite in screwing direction; the first sliding block is connected to the outer side of the rotating screw rod in a sleeving mode and is in threaded connection with the first external thread, and the second sliding block is connected to the outer side of the rotating screw rod in a sleeving mode and is in threaded connection with the second external thread; and the top ends of the first sliding block and the second sliding block are fixedly connected with fixing clamps.
Owner:HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
Rapid preparation method for lumen grinding disc of deep-sea foraminifera
ActiveCN110243640AIncrease success rateClear imagingPreparing sample for investigationPaleoceanographyEnvironment of Albania
The invention relates to the disciplines of marine biology, paleoceanography and sedimentary geology, can be applied to the field of the identification research of the species of foraminifera, the research of marine environment indication, the research of paleoenvironmental reconstruction and global change, and the like, and in particular provides a rapid preparation method for a lumen structure grinding disc of deep-sea foraminifera. The foraminifera obtained from the separation of deep-sea sediments are subjected to gum dipping lumen, hanging drop embedding, positioning fixation and water-grinding disc processing, that is, a complete grinding disc of the lumen structure of the deep-sea foraminifera is quickly obtained. The method provided by the invention, through the layer information obtained by the grinding disc, can more accurately identify the species of foraminifera, avoids scientific confusion such as identification errors caused by external morphological variations such as the shape, texture and number of compartments of the worm body, and provides accurate scientific basis and quick problem solving solutions for the disciplines of accurate identification of the species of deep-sea foraminifera, the research thereof of marine environment indication, the research of paleoenvironmental reconstruction, the research of global change, and the like.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI +1
A method for cryopreservation and activation of spermatozoa of ovoviviparous fish Scorpene xushi
ActiveCN109819976BEnrich planting resource conservation methodsImprove stabilityDead animal preservationMarine fishSaltwater fish
The invention belongs to the technical field of marine biology, and in particular relates to a method for ultra-low temperature preservation and activation of sperm of the ovoviviparous fish Scorpionus scorpionus. The sperm obtained from the mature stage of the sperm of the male scorpionfish of Xu's flatfish is evenly mixed with the freezing liquid in the ratio of 1:4-1:5, and then the gradient cooling is carried out for freezing, and then the frozen mixed liquid is moved to liquid nitrogen, that is, the sperm freezing is realized. Ultra-low temperature long-term storage; the gradient cooling freezing is that the mixed solution is pre-cooled at 0-4°C for 2-5min, then frozen to -80-100°C at a speed of 10-12°C / min, and then 15-18°C / min Freeze at ‑150°C. The invention establishes a method for ultra-low temperature preservation of ovoviviparous fish sperm and a sperm activation method, and provides a new method and a new idea for cultivating new varieties of marine fish.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Universal rpa nucleic acid isothermal amplification primers, kits and applications for bivalve mollusk-infected herpes virus oshv-1
ActiveCN113293239BStrong specificityHigh sensitivityMicrobiological testing/measurementMicroorganism based processesBivalve shellHerpes simplex virus DNA
The invention belongs to the technical field of marine biology, and in particular relates to a universal RPA nucleic acid isothermal amplification primer, a kit and an application thereof for bivalve mollusks infected with herpes virus OsHV‑1. The primers are a set of amplification primers, consisting of upstream and downstream primers and probe primers; the upstream primers are shown in SEQ ID NO.1; the downstream primers are shown in SEQ ID NO.2; the probe primers are shown in SEQ ID NO.3 shown. The present invention designs general-purpose RPA amplification detection primers for 7 kinds of OsHV-1 mutant strains in currently known four kinds of bivalve hosts, and establishes a test strip RPA detection system and method for OsHV-1. On-site rapid screening and detection of OsHV‑1 is of great value and has broad market application prospects.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Rapid Preparation Method of Internal Cavity Polishing of Deep-sea Foraminifera
ActiveCN110243640BIncrease success rateClear imagingPreparing sample for investigationMicroporaSediment
The present invention relates to marine biology, paleoceanography, sedimentary geology and other disciplines, and can be applied in the fields of foraminifer species identification research, marine environment indication research, paleoenvironment reconstruction and global change research, etc. Specifically, it is a A rapid preparation method for deep-sea foraminifera lumen structure grinding plate. Foraminifera isolated from deep-sea sediments were treated with gum immersion, hanging drop embedding, position adjustment and water grinding, that is, complete grinding of the inner cavity structure of deep-sea foraminifera was quickly obtained. The invention can more accurately identify the species of the foraminifera through the level information obtained by grinding, and avoid scientific confusion such as identification errors caused by external morphological variations such as the shape, texture, and number of chambers of the foraminifera. The invention provides accurate scientific basis and quick problem-solving scheme for the accurate identification of types of deep-sea foraminifera and the research on indications to the marine environment, paleoenvironment reconstruction research, global change research and other subject fields.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI +1
Application of disodium ethylenediamine tetraacetate as drug for treating jellyfish dermatitis
PendingCN109464434ASuppress deathInhibit expressionOrganic active ingredientsAntipyreticEthylenediamineInflammatory factors
The invention relates to the technical field of marine biology, in particular to application of disodium ethylenediamine tetraacetate (non-specific metalloprotease inhibitor) as a drug for treating jellyfish dermatitis. The invention provides application of EDTA-2Na as a drug for treating jellyfish dermatitis. According to the invention, disodium ethylenediamine tetraacetate can effectively inhibit the death of two types of cells (human keratinocyte HaCaT and human embryonic fibroblast CCC-ESF-1) and the expression amount of an inflammatory factor mRNA caused by physaliatoxin, and further canbe used as a drug for treating jellyfish dermatitis, so that the application range of EDTA-2Na is widened.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
A marine natural gas hydrate development environment monitoring system and method
Owner:GUANGZHOU MARINE GEOLOGICAL SURVEY
A Cetacean Whistle Signal Detection Method Based on Mel Subband Spectral Entropy
Owner:HARBIN ENG UNIV
A method for rapidly screening microbial strains producing tetrodotoxin and the used digoxin-labeled dna probe
ActiveCN108977505BHigh precisionReduce mistakesMicrobiological testing/measurementDNA/RNA fragmentationSingle strandBacterial strain
The invention relates to the technical field of marine biology, in particular to a method for rapidly screening microbial strains producing tetrodotoxin and a digoxin-labeled DNA probe used therefor, the probe used in the method is 254 complementary to the DNA base to be tested Base-length single-stranded DNA, the 3' end of the probe is labeled with digoxigenin. Through colony in situ dot molecular hybridization, the probe can specifically detect whether there is tetrodotoxin synthesis sxt gene nucleic acid in the DNA sample of the strain to be tested, and screen out toxin-producing positive strains. The method involved in the invention utilizes the in situ extract of bacterial strain DNA to perform colony dot hybridization, and can complete the screening of 96 samples within 8-10 hours. The method of the invention is fast, has strong specificity and high accuracy.
Owner:ZHEJIANG OCEAN UNIV
Sea anemone Kunitz-type protease inhibitor and application thereof
InactiveCN103387614BEnhanced inhibitory effectBiocidePeptide/protein ingredientsADAMTS ProteinsChemical weapon
The invention relates to the technical field of marine biology. The sea anemone contains a lot of polypeptide toxins and proteins, such as toxin compounds and protease inhibitors, and especially, palytoxin in the nematocysts of the sea anemone, is believed to be a chemical weapon for defense. Currently, the studies on a sea anemone Kunitz-type protease inhibitor and the functions thereof are very limited. The present invention provides a sea anemone derived Kunitz-type protease inhibitor, the amino acid sequence of the protease inhibitor is shown in SEQ ID NO: 2. The present invention also provides the encoding gene of the protease inhibitor, and applications of the protease inhibitor in the preparation of drugs for the treatment of acute pancreatitis and acute hemorrhagic pharmaceutical, and pesticides.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
A kind of preparation method and application of ethanol extract of Cryptocodella sp.
ActiveCN107156844BEasy to removeLower levelAlgae medical ingredientsAntinoxious agentsDismutaseIn vivo
The invention belongs to the technical field of marine organisms, and provides a preparation method and application of ethanol extract of Cryptocodella sp. The ethanol extract of cryptocoleum provided by the invention is obtained by repeatedly freezing and thawing fresh cryptocoleum wet algae, centrifuging to take the precipitate and adding ethanol for ultrasonic extraction, and the obtained cryptocoleum ethanol extract can Significantly improve the activity of superoxide dismutase (SOD) in Caenorhabditis elegans and promote the removal of reactive oxygen species (ROS) in the body, reduce the level of reactive oxygen species (ROS) in the body, and at the same time it can alleviate the oxidative stress caused by premature aging phenomenon, has a significant antioxidant effect, and can be applied to the preparation of health food and medicines with antioxidant functions.
Owner:GUANGDONG PHARMA UNIV
A method for rapid screening of microbial strains producing gonitoxin and the used digoxin-labeled dna probe
ActiveCN108977506BHigh precisionReduce mistakesMicrobiological testing/measurementDNA/RNA fragmentationSingle strandBacterial strain
The invention relates to the technical field of marine biology, in particular to a method for rapidly screening microbial strains producing gonitoxins and a digoxin-labeled DNA probe used therein. The probe used in the method is complementary to the DNA base to be tested 248 bases long single-stranded DNA, the 3' end of the probe is labeled with digoxigenin. Through colony in situ dot molecular hybridization, the probe can specifically detect whether there is sxtS gene nucleic acid in the DNA sample of the strain to be tested, and screen out toxin-producing positive strains. The method involved in the invention utilizes the in situ extract of bacterial strain DNA to perform colony dot hybridization, and can complete the screening of 96 samples within 8-10 hours. The method of the invention is fast, has strong specificity and high accuracy.
Owner:ZHEJIANG OCEAN UNIV
A method for rapid screening of microbial strains producing fin algae toxins
ActiveCN108977504BLow costImprove accuracyMicrobiological testing/measurementBiotechnologyMicroorganism
The invention relates to the technical field of marine biology, and in particular to a method for rapidly screening microbial strains producing fin algae, comprising the following steps: (1) pretreatment: making and fixing the DNA of the strain to be tested; (2) hybridization: mixing the strain to be tested The DNA of the strain is hybridized with the labeled single-stranded DNA probe; (3) Screening: Whether the strain to be tested produces finnolyxin is specifically detected. The invention can specifically detect whether the DNA sample of the strain to be tested produces pinotoxin through the in situ spot molecular hybridization of the colony, and the labeled single-stranded DNA probe has the characteristics of low cost, rapidity, high accuracy and sensitivity; low analysis cost, cycle time The reagents used are green and environmentally friendly, harmless to the human body, and have high market prospects and economic value.
Owner:ZHEJIANG OCEAN UNIV
A jellyfish hairpin polypeptide growth factor and its preparation method and application
The invention belongs to the technical field of marine biology, and provides a cyanea capillata polypeptide growth factor named as Cc-GRN-1. The amino sequence of the growth factor is represented by the SEQ ID No. 1. The invention also provides a preparation method and applications of the cyanea capillata polypeptide growth factor. The cyanea capillata polypeptide growth factor has a prominent promoting effect on cell proliferation, and has a good application prospect in development of damage repairing drugs.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Seaweed product for agriculture and preparation method of fucoidin
InactiveCN100457783CMaintain biological activityImprove qualityBiocideAnimal repellantsFodderDrug biological activity
The invention discloses a comprehensive utilization usage of seaweed; especially production method of involving producing seaweed agricultural product and fucosido- polysaccharide; which comprise the following parts: adopting marine biology sodium as main material; applying freezing disintegrating technology, ultrasonic wave water and lixivium extraction steps; extracting inherent autogenous plant growing modifier in maximum limit and keeping biological activity; separating fucosido-polysaccharide and producing bottom of last leaving to fertilizer, soil modifier and fodder; that can be using fully.
Owner:YANTAI DIYUAN BIOTECH
Hermatypic coral symbiotic zooxanthellae algal species as well as separation and purification method and culture method thereof
The invention relates to the research field of coral symbiotic algae in marine biology, in particular to a hermatypic coral symbiotic zooxanthellae algal species and a separation and purification method and a culture method thereof. According to the coral and zooxanthellae symbiotic characteristics and the corresponding physiological mechanism, separation, purification and in-vitro culture are performed on zooxanthellae in the coral in a targeted manner, according to the close symbiotic relationship of the zooxanthellae, impurities are not removed in the initial stage of culture, so that the zooxanthellae can gradually adapt to the in-vitro culture environment, and after entering the index growth period, the zooxanthellae can be separated and purified. And separating and purifying to obtain the pure hermatypic coral symbiotic zooxanthellae algal species. The research result of the invention is to promote the physiological function of zooxanthellae and research the symbiotic relationship between coral and zooxanthellae, and provides a feasible thought for protecting the coral reef ecosystem.
Owner:HAINAN UNIVERSITY
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