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Composition for inhibiting skin decomposition of stichopus japonicus and application

A technology of composition and application method, which is applied in the fields of application, climate change adaptation, fish farming, etc., and can solve the problems of short fresh life of sea cucumber, complicated operation of anesthesia, inability to alleviate the impact of sea cucumber, etc.

Active Publication Date: 2021-12-24
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used ways to delay the transformation of sea cucumbers in actual production mainly include low temperature, oxygenation and anesthesia. The method is complicated to operate, and it needs to be raised for a period of time before it can be sold

Method used

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  • Composition for inhibiting skin decomposition of stichopus japonicus and application
  • Composition for inhibiting skin decomposition of stichopus japonicus and application
  • Composition for inhibiting skin decomposition of stichopus japonicus and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Construction of peeling model: 10 fresh sea cucumbers were taken and divided into two groups, UVA irradiation group and control group. The sea cucumbers in the UVA irradiation group were drained of seawater, and the radiation intensity was 15W / m 2 , irradiated with a UVA lamp with a wavelength of 365nm for 40min, and then placed on ice and protected from light. The sea cucumbers in the control group were drained of seawater and stored on ice in the dark without any treatment. Observe and record the number of peeling spots of A. japonicus every 12 hours.

[0014] Data analysis: Use the R language aov function to conduct one-way analysis of variance, and use the aggregate function to calculate the mean and standard deviation. The results showed that there was a significant difference between the UVA irradiation group and the control group from the 12h recording, as shown in Table 1, and at 96h, the skinning degree of the UVA irradiation group was significantly higher th...

Embodiment 2

[0064] After the usage methods and effects of different inhibitors are obtained by the above method, in order to obtain the inhibitor with the best effect of inhibiting peeling, the inhibitors are used in combination.

[0065] 1.1 Grouping:

[0066] According to the effect of the above-mentioned different inhibitors, the following groups were selected: A, Emricasan+Ilomastat+Nafamostat Mesylate soaking group (3 rats), B, Emricasan soaking group (3 rats), C, Ilomastat+NafamostatMesylate soaking group (3 rats), D. Control group (3 rats).

[0067] 2.2 Solution configuration

[0068] Inhibitor A: Emricasan at an initial concentration of 5 mM, Ilomastat at 10 mM, and Nafamostat Mesylate at 10 mg / ml were mixed and diluted with low-temperature ultra-filtered seawater to form A solvent. The final concentrations of its constituents are Emricasan 30 μM, Ilomastat 20 μM, and Nafamostat Mesylate 15ug / ml.

[0069] B Inhibitor: Emricasan at an initial concentration of 5 mM. Use low-temp...

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Abstract

The invention relates to a composition for inhibiting skin degradation of stichopus japonicus and application, and belongs to the technical field of marine biology. The composition is prepared from, by final concentration, 30 [mu] M of Emrican, 20 [mu] M of Ilomastat and 15 [mu] g / ml of Nafamostat Mesylate, the volume ratio of DMSO is 1%, and a solvent is ultrafiltration seawater. Fresh and live sea cucumbers are soaked in a solution composed of the composition at 4 DEG C for 45 min. The soaked fresh and live sea cucumbers can be placed on ice to be preserved for 96 hours without autolysis after being irradiated by a UVA lamp with the radiation intensity being 15 W / m<2> and the wavelength being 365 nm for 40 minutes.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a composition and application for inhibiting the peeling of sea cucumbers. Background technique [0002] Sea cucumber (Apostichopus japonicus) belongs to the phylum Echinodermata, class Holothuria, order Apostichopidae, and family Apostichopidae. It has extremely high nutritional value and economic value, and is the main species of sea cucumber cultured in my country. The freshness of sea cucumber affects its quality, price and reculture to a certain extent. Apostichopus is stimulated by the external environment, such as ultraviolet radiation, high temperature, trauma, oil stains, etc., which will produce skin peeling and cause serious economic losses. Therefore, in scientific research and actual production, it is an important problem to be solved urgently to maintain its freshness and prolong its storage and transportation time by inhibiting the autolysis o...

Claims

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Application Information

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IPC IPC(8): A01K61/30
CPCA01K61/30Y02A40/81
Inventor 丁君常亚青国超王荦尹东红
Owner DALIAN OCEAN UNIV
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