15 results about "Apostichopus" patented technology

The invention relates to the technical field of genomics, molecular biology, bioinformatics and whole-genome selective breeding, in particular to a whole-genome liquid chip for apostichopus japonicus and application of the whole-genome liquid chip. The liquid phase chip contains sequences of background SNP loci and functional SNP loci which are used for gene analysis and are positioned on an apostichopus japonicus reference genome; wherein the functional SNP sites are associated with important economic characters of the apostichopus japonicus. The invention also discloses application of the liquid chip in apostichopus japonicus genome selective breeding, important economic character gene positioning, genetic diversity analysis, germplasm resource improvement and protection.

This invention relates to the fields of genomics, molecular biology, bioinformatics, and genome-wide selection breeding, specifically a 20k liquid-phase breeding chip for the whole genome of *S. esculenta* and its applications. The liquid-phase chip contains background SNPs and functional SNPs located on the *S. esculenta* reference genome; wherein the background SNPs are uniformly distributed within the genome; and the functional SNPs are associated with important economic traits of *S. esculenta*; these important economic traits include one or more of the following: saponin content, polysaccharide content, and heat tolerance. The chip can be applied to the assessment of genetic diversity in *S. esculenta*, identification of germplasm resources and phylogenetic relationships, genome-wide association analysis of important economic traits, and genome-wide selection breeding. This chip has advantages such as high throughput, high region coverage, high locus detection rate, and high flexibility, providing powerful tool support for molecular breeding of *S. esculenta*.

The invention belongs to the technical field of molecular genetics, and relates to a stichopus japonicus whole life history on-site rapid sex determination molecular marker, a kit and application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table. The stichopus japonicus with the molecular marker is male. The invention also provides a primer for amplifying the molecular marker, a loop-mediated isothermal amplification detection primer group, a kit, an identification method and the like. The molecular marker and the developed kit can be used for rapid sex identification of the stichopus japonicus in the whole life history, and the problem of accurate sex identification in stichopus japonicus culture is solved.

The application belongs to the technical field of microorganisms, and discloses a lactobacillus rhamnosus for relieving cadmium heavy metal toxicity and application thereof. The lactobacillus rhamnosus is preserved in the China Center for Type Culture Collection, and the address of the preservation is Wuhan University in Hubei Province, and the preservation number is CCTCC No. M20241815. The lactobacillus rhamnosus provided by the application is derived from the intestinal tract of healthy Apostichopus japonicus, has good cadmium heavy metal tolerance and removal capacity, and is expected to be developed and applied in the health food industry.

The application discloses a lactococcus lactis GL-1 and application of the lactococcus lactis GL-1 in Apostichopus japonicus breeding, and belongs to the field of aquatic beneficial microorganisms and application technologies thereof. The lactococcus lactis GL-1 is preserved in the China General Microbiological Culture Collection Center on September 16, 2025, and the preservation number is CGMCC No. 35938, and the lactococcus lactis GL-1 is isolated from traditional fermented food doubansauce. The strain can well adapt to different environments, and can be used for preparing an Apostichopus japonicus feed additive. When the number of viable lactococcus lactis GL-1 in the feed is greater than or equal to 1x10 8 CFU / g, the low-salt stress resistance of the Apostichopus japonicus can be significantly enhanced, the survival rate and growth performance are improved, the intestinal flora structure is improved, the colonization of beneficial bacteria is promoted, and immune-related pathways such as the Wnt signal pathway are activated, so that the health level of the Apostichopus japonicus is improved from multiple aspects. A safe and effective microbial solution is provided for solving the low-salt stress problem in the Apostichopus japonicus breeding.

The application discloses an antagonistic bacterium of pathogenic shewanella of Apostichopus japonicus and application thereof, and belongs to the technical field of aquatic microorganisms. Gilvus japonicus The antagonistic bacterium is separated from the intestinal tract of healthy Apostichopus japonicus and is identified as Japanese Yellow Sea Oceanic Bacterium (Shewanella japonica), has no beta-hemolysis characteristic, is safe and non-toxic to Apostichopus japonicus, and can significantly antagonize the pathogenic bacterium Shewanella of Apostichopus japonicus skin rot syndrome. The application also discloses application of the antagonistic bacterium, which can be prepared into an aquatic microecological preparation for preventing and treating the skin rot syndrome of Apostichopus japonicus, and is applied through intraperitoneal injection or immersion, so that the survival rate of Apostichopus japonicus under exposure of the pathogenic bacterium is significantly improved. The strain has strong salt and alkali resistance, tolerances to various antibiotics and chemical drugs, and strong environmental adaptability, can be applied to the cultivation of Apostichopus japonicus to replace traditional antibiotics, effectively solves the problems of food safety and environmental pollution caused by the abuse of antibiotics, and has good industrial application prospect.

The application discloses an antioxidant peptide from Apostichopus japonicus and a preparation method and application thereof, and relates to the technical field of biological medicines. The antioxidant peptide from Apostichopus japonicus has an amino acid sequence as shown in SEQ ID NO. 1. The antioxidant peptide with the amino acid sequence of LPPF is screened from the Apostichopus japonicus, and the peptide is verified to have excellent antioxidant activity and high safety, and has a good application prospect in the treatment of oxidative stress injury diseases.

The application discloses a sea cucumber polysaccharide cancer inhibiting preparation and a preparation method and application thereof, and belongs to the technical field of biological medicine. The preparation is prepared from high-purity sea cucumber polysaccharide extracted from Apostichopus japonicus as a core active ingredient, and is matched with a folic acid modified polyethylene glycol-poly lactic acid copolymer targeting carrier and medicinal adjuvants, and is prepared through steps of standardization extraction and purification, targeting carrier preparation, granulation and coating. The application adopts an enzymolysis process combining with self-lyase and exogenous enzyme, and cooperates with multi-step purification and ultrafiltration grading technology to precisely control the purity and molecular weight of the polysaccharide, realizes active targeting of tumor cells through the FA-PEG-PLA targeting carrier, and improves the bioavailability through enteric coating technology. The preparation can up-regulate the expression of TNFR1 protein, activates the caspase cascade reaction to induce tumor cell apoptosis, has a significant inhibiting effect on solid tumors such as lung cancer, kidney cancer and liver cancer, and has very low toxicity to normal cells.

This invention discloses a sea cucumber farming device and method, comprising a base; a biomimetic reef body disposed on the base; an anti-siltation layer disposed on the base and surrounding the outside of the biomimetic reef body; and a liftable farming fence surrounding the anti-siltation layer, with a gap between them. The liftable farming fence includes several sets of movable net panels and a driving component. Each set of movable net panels includes multiple mesh plates, with adjacent mesh plates slidably connected to each other. The bottom of the mesh plate closest to the base is fixedly connected to the base, while the remaining mesh plates slide through the driving component. This invention provides a sea cucumber farming device. The liftable farming fence can adjust its height according to different growth stages of the sea cucumber, improving farming density and space utilization, and providing efficient feed supply. The anti-siltation layer, combined with tidal action, achieves self-cleaning, effectively removing silt, reducing the growth of harmful organisms, lowering the incidence of diseases, and improving the survival rate.

The present application belongs to the technical field of molecular genetics, and relates to a genetic sex molecular marker of Apostichopus japonicus, a primer, a method, a kit and application. The nucleotide sequence of the molecular marker is shown in the sequence table SEQ ID NO. 1. The Apostichopus japonicus with the molecular marker is male. The present application also provides a primer for amplifying the molecular marker, a loop-mediated isothermal amplification detection primer group and reagent or kit, an identification method and the like. The molecular marker and the developed kit can be used to carry out sex identification of Apostichopus japonicus, and solve the problem of accurate identification of male and female in Apostichopus japonicus breeding.

The application provides a kind of umami peptide derived from Apostichopus japonicus, amino acid sequence is any one of Glu-Gly-Ala-Thr (EGAT), Gly-Ala-Asp-Ala (GADA), Asp-Ser-Ala-Thr (DSAT), Asp-Ser-Thr-Thr (DSTT) or Ser-Ser-Ala-Glu (SSAE).The application also provides the preparation method, identification method and application of the umami peptide.The application adopts step-by-step enzymatic hydrolysis method, through raw material pretreatment, Alcalase enzyme first-step enzymatic hydrolysis, flavourzyme second-step enzymatic hydrolysis, sterilization centrifugation, ultrafiltration separation, gel chromatography separation and purification steps, to obtain umami peptide;through the integration identification strategy of "electronic tongue preliminary screening-peptide sequence identification-virtual screening-molecular docking simulation-electronic tongue taste verification", five tetrapeptides with umami activity EGAT, GADA, DSAT, DSTT and SAEA are identified from Apostichopus japonicus protease hydrolysate for the first time, which have small molecular weight, confirmed to have pure umami, and weak bitter taste, sour taste and other undesirable flavors;the umami peptide can be used for preparing food condiment, nutritional enhancer or flavor enhancer;belongs to the field of food science and bioactive peptide technology.

This invention discloses a method for predicting the spawning period of *Stichopus japonicus*, belonging to the field of *Stichopus japonicus* aquaculture data prediction. This invention analyzes the correlation between body weight, accumulated temperature, and changes in total saponin content in the gonads of female *Stichopus japonicus* during a period before and after spawning, constructs a model of the relationship between changes in total saponin content in the gonads and the timing of egg release, and establishes a method for accurately predicting the spawning timing of *Stichopus japonicus*. Using this prediction method, the spawning timing of *Stichopus japonicus* can be accurately determined, solving the problem of not being able to accurately predict the spawning timing for timely harvesting. Producers can then plan the water body used for seedling cultivation in their workshops.

The invention belongs to the technical field of molecular genetics, and relates to an apostichopus japonicus genetic sex molecular marker, a primer, a method, a kit and application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table. The apostichopus japonicus with the molecular marker is male. The invention also provides a primer for amplifying the molecular marker, a loop-mediated isothermal amplification detection primer group, a reagent or a kit, an identification method and the like. By utilizing the molecular marker and the developed kit, the sex identification of the apostichopus japonicus can be carried out, and the problem of accurate male and female identification in the culture of the apostichopus japonicus is solved.

The invention discloses lactococcus lactis GL-1 and application of the lactococcus lactis GL-1 in stichopus japonicus culture, and belongs to the technical field of aquatic product beneficial microorganisms and application of the aquatic product beneficial microorganisms. The lactococcus lactis GL-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 16, 2025, the preservation number is CGMCC No.35938, and the lactococcus lactis GL-1 is separated from the traditional fermented food thick broad-bean sauce. The strain can well adapt to different environments and can be used for preparing stichopus japonicus feed additives, and when the viable count of lactococcus lactis GL-1 in feed is larger than or equal to 1 * 10 < 8 > CFU / g, the low-salt stress resistance of stichopus japonicus can be remarkably improved, and the survival rate and the growth performance can be improved; meanwhile, the intestinal flora structure is improved, beneficial bacterium colonization is promoted, immune-related pathways such as a Wnt signal pathway are activated, and the health level of the stichopus japonicus is improved from multiple levels. A safe and effective microbial solution is provided for solving the problem of low salt stress in stichopus japonicus culture.

The invention provides lactobacillus casei and application thereof, the lactobacillus casei is separated and screened from the intestinal tract of apostichopus japonicus, has a promoting effect on the intestinal health of animals, and can increase the number of beneficial bacteria in the intestinal tract of the animals and inhibit the growth of harmful bacteria, so that the incidence rate of intestinal diseases of the animals is reduced; the disease resistance of animals can be improved, the immunity of the organism is enhanced, and the infection rate of viruses and bacteria on the animals is reduced. The lactobacillus casei is an endogenous intestinal probiotic, is not only suitable for growth and reproduction in a marine environment, but also has no danger to bred animals and human beings, and has high efficiency and safety in the aspect of application. The lactobacillus casei disclosed by the invention has the advantages of low cost, simplicity and convenience in operation and better commercial prospect.