34 results about "Apostichopus" patented technology

The invention belongs to the technical field of food biology, and particularly relates to a sea cucumber-derived antioxidant peptide as well as a preparation method and application thereof. The sea cucumber source antioxidant peptide is prepared and screened from apostichopus japonicus and comprises at least one of peptides with the amino acid sequence shown as SEQ ID NO.1-4, and the docking energy of the peptides and a protein receptor Keap1 is-8.6 kcal / mol,-9.4 kcal / mol,-9.1 kcal / mol and-8.8 kcal / mol respectively. The antioxidant peptide obtained by the invention can be applied to food, cosmetics or medicines, and has important significance on the development of novel antioxidant products.

The invention relates to the technical field of genomics, molecular biology, bioinformatics and whole-genome selective breeding, in particular to a whole-genome liquid chip for apostichopus japonicus and application of the whole-genome liquid chip. The liquid phase chip contains sequences of background SNP loci and functional SNP loci which are used for gene analysis and are positioned on an apostichopus japonicus reference genome; wherein the functional SNP sites are associated with important economic characters of the apostichopus japonicus. The invention also discloses application of the liquid chip in apostichopus japonicus genome selective breeding, important economic character gene positioning, genetic diversity analysis, germplasm resource improvement and protection.

This invention relates to the fields of genomics, molecular biology, bioinformatics, and genome-wide selection breeding, specifically a 20k liquid-phase breeding chip for the whole genome of *S. esculenta* and its applications. The liquid-phase chip contains background SNPs and functional SNPs located on the *S. esculenta* reference genome; wherein the background SNPs are uniformly distributed within the genome; and the functional SNPs are associated with important economic traits of *S. esculenta*; these important economic traits include one or more of the following: saponin content, polysaccharide content, and heat tolerance. The chip can be applied to the assessment of genetic diversity in *S. esculenta*, identification of germplasm resources and phylogenetic relationships, genome-wide association analysis of important economic traits, and genome-wide selection breeding. This chip has advantages such as high throughput, high region coverage, high locus detection rate, and high flexibility, providing powerful tool support for molecular breeding of *S. esculenta*.

The invention belongs to the technical field of molecular genetics, and relates to a stichopus japonicus whole life history on-site rapid sex determination molecular marker, a kit and application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table. The stichopus japonicus with the molecular marker is male. The invention also provides a primer for amplifying the molecular marker, a loop-mediated isothermal amplification detection primer group, a kit, an identification method and the like. The molecular marker and the developed kit can be used for rapid sex identification of the stichopus japonicus in the whole life history, and the problem of accurate sex identification in stichopus japonicus culture is solved.

The invention relates to the technical field of stichopus japonicus breeding, in particular to a constant-temperature stichopus japonicus breeding box. Comprising a support frame, the breeding net is arranged on the supporting frame; the floating ball is arranged on the supporting frame; the mounting plate is arranged on the supporting frame, the mounting plate is provided with a pneumatic sleeve, the pneumatic sleeve is connected with a pneumatic sliding rod in a sliding mode, and the pneumatic sliding rod is fixedly connected with a pull rope; the first winding shaft is rotationally connected to the mounting plate; and the second winding shaft is rotationally connected to the mounting plate, and a pull rope is wound around the second winding shaft. The second winding shaft rotates through movement of the pneumatic sliding rod and a series of transmission, then the second winding shaft releases the pull rope, the height of the culture net is adjusted, the position of the culture net is adjusted, stichopus japonicus in the culture net is in a proper temperature range all the time, and the growth efficiency of the stichopus japonicus is guaranteed.

The application belongs to the technical field of microorganisms, and discloses a lactobacillus rhamnosus for relieving cadmium heavy metal toxicity and application thereof. The lactobacillus rhamnosus is preserved in the China Center for Type Culture Collection, and the address of the preservation is Wuhan University in Hubei Province, and the preservation number is CCTCC No. M20241815. The lactobacillus rhamnosus provided by the application is derived from the intestinal tract of healthy Apostichopus japonicus, has good cadmium heavy metal tolerance and removal capacity, and is expected to be developed and applied in the health food industry.

The invention discloses a method for transferring stichopus japonicus into a pond and a box instead of antibiotics, and belongs to the technical field of stichopus japonicus culture. Every time the pond or the box is reversed, the hypochlorous acid disinfectant is diluted and then is used for soaking the sea cucumbers, and after soaking is finished, the soaking solution containing the hypochlorous acid disinfectant is poured into the culture water together with the hypochlorous acid disinfectant. According to the method for transferring the stichopus japonicus into the pond and the box instead of antibiotics, the body surface and pathogens in the body of the stichopus japonicus can be killed after treatment, and the stichopus japonicus with enteritis can be helped to relieve inflammation and relieve enteritis. The growth speed of the stichopus japonicus using the method is higher than that of a control group. And the cost of using hypochlorous acid is 20-30% lower than that After treatment in the embodiments 1-3, the growth speed is more than two times that of a control group. The stichopus japonicus fry with enteritis diseases are soaked in 0.2% hypochlorous acid gel for 30 minutes, 99% of the stichopus japonicus fry are recovered to be healthy once and three times a day, and the method is simple, convenient, easy to implement by farmers and relatively low in cost.

The application discloses a lactococcus lactis GL-1 and application of the lactococcus lactis GL-1 in Apostichopus japonicus breeding, and belongs to the field of aquatic beneficial microorganisms and application technologies thereof. The lactococcus lactis GL-1 is preserved in the China General Microbiological Culture Collection Center on September 16, 2025, and the preservation number is CGMCC No. 35938, and the lactococcus lactis GL-1 is isolated from traditional fermented food doubansauce. The strain can well adapt to different environments, and can be used for preparing an Apostichopus japonicus feed additive. When the number of viable lactococcus lactis GL-1 in the feed is greater than or equal to 1x10 8 CFU / g, the low-salt stress resistance of the Apostichopus japonicus can be significantly enhanced, the survival rate and growth performance are improved, the intestinal flora structure is improved, the colonization of beneficial bacteria is promoted, and immune-related pathways such as the Wnt signal pathway are activated, so that the health level of the Apostichopus japonicus is improved from multiple aspects. A safe and effective microbial solution is provided for solving the low-salt stress problem in the Apostichopus japonicus breeding.

The invention discloses an apostichopus japonicus extract antioxidant peptide as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The amino acid sequence of the apostichopus japonicus essence antioxidant peptide is shown as SEQ ID NO. 1. The apostichopus japonicus essence antioxidant peptide has the advantages that the apostichopus japonicus essence antioxidant peptide is prepared; the antioxidant peptide with the amino acid sequence of LPPF is screened from the apostichopus japonicus essence, and the peptide is proved to have excellent antioxidant activity and high safety, and has a better application prospect in treatment of oxidative stress injury diseases.

The invention relates to application of biotin in preparation of feed or medicine for inhibiting bacteria and increasing apostichopus japonicus cell phagocytic activity, and belongs to the field of aquaculture, the application method is as follows: biotin is added into the medicine or feed to improve expression of superoxide dismutase and ferritin genes in apostichopus japonicus bodies, cells are protected from oxidative damage, and the apostichopus japonicus cell phagocytic activity is improved. Meanwhile, the expression of c3 and tlr3 genes is up-regulated, and the recognition capability of the stichopus japonicus on exogenous pathogens is promoted, so that the non-specific immunity of the stichopus japonicus can be remarkably enhanced, and the effect of preventing the skin ulceration syndrome of the stichopus japonicus is achieved.

The invention provides stichopus japonicus breeding feed and a stichopus japonicus breeding method, and belongs to the technical field of stichopus japonicus breeding. The rape seed cake meal is adopted for providing vegetable protein, amino acid and trace elements such as manganese, zinc and copper, the algae powder is adopted for providing amino acid, vitamins and organic mineral substances, the undaria pinnatifida powder and the crab shell powder contain rich protein and mineral substances, and the undaria pinnatifida powder, the crab shell powder and the undaria pinnatifida powder can meet nutrient substances needed by growth of the stichopus japonicus; the multivitamins can activate the non-specific immune response of the stichopus japonicus, enhance the anti-stress and disease-resistant capabilities of the body, and cooperate with the auxiliary nutrients to improve the metabolism of the stichopus japonicus, promote the healthy growth of the stichopus japonicus and improve the yield and quality of the stichopus japonicus; and the stichopus japonicus culture feed is solid particles in the shape of carambola, so that the stichopus japonicus culture feed can quickly sink into water, a plurality of edges of the stichopus japonicus culture feed can be quickly softened after meeting water, and the middle part of the stichopus japonicus culture feed can still keep the shape for a long time, so that the stichopus japonicus can quickly ingest from the plurality of edges and then gradually ingest the middle part, and the ingestion time is prolonged.

The application discloses an antagonistic bacterium of pathogenic shewanella of Apostichopus japonicus and application thereof, and belongs to the technical field of aquatic microorganisms. Gilvus japonicus The antagonistic bacterium is separated from the intestinal tract of healthy Apostichopus japonicus and is identified as Japanese Yellow Sea Oceanic Bacterium (Shewanella japonica), has no beta-hemolysis characteristic, is safe and non-toxic to Apostichopus japonicus, and can significantly antagonize the pathogenic bacterium Shewanella of Apostichopus japonicus skin rot syndrome. The application also discloses application of the antagonistic bacterium, which can be prepared into an aquatic microecological preparation for preventing and treating the skin rot syndrome of Apostichopus japonicus, and is applied through intraperitoneal injection or immersion, so that the survival rate of Apostichopus japonicus under exposure of the pathogenic bacterium is significantly improved. The strain has strong salt and alkali resistance, tolerances to various antibiotics and chemical drugs, and strong environmental adaptability, can be applied to the cultivation of Apostichopus japonicus to replace traditional antibiotics, effectively solves the problems of food safety and environmental pollution caused by the abuse of antibiotics, and has good industrial application prospect.

The invention provides a preparation method and application of a pomegranate rind extract, and the preparation method comprises the following steps: crushing pomegranate rind by a crusher, sieving, soaking in double distilled water, boiling with big fire, boiling with small fire for 20-60 minutes, and concentrating with big fire to obtain the pomegranate rind extract. Compared with the prior art, the pomegranate peel single Chinese herbal medicine aqueous extract prepared by the invention has a remarkable inhibition effect on apostichopus japonicus skin ulcer pathogenic bacteria photobacterium damsonii, is relatively low in toxicity, and can be used for preventing and controlling apostichopus japonicus skin ulcer syndrome caused by the photobacterium damsonii. Moreover, a proper amount of pomegranate peel aqueous extract is mixed with bait to feed the apostichopus japonicus, so that the capability of resisting photobacterium damsonii subspecies infection of the apostichopus japonicus can be remarkably enhanced, and the survival rate of the apostichopus japonicus is increased.

The present invention discloses microsatellite markers related to the early body color of Apostichopus japonicus and their applications, belonging to the technical field of molecular markers. Two microsatellite sequences screened based on the genome of Apostichopus japonicus in the present invention are respectively numbered AJ732 and AJ812, and their sequences are shown in SEQ ID NO.1 and SEQ ID NO.2 in sequence. Microsatellite marker primers are respectively designed for the two microsatellite sequences. The microsatellite marker primers have strong specificity and stable amplification, can be used to judge the body color traits of Apostichopus japonicus larvae, can improve the breeding efficiency and accuracy of Apostichopus japonicus populations, and provide a powerful tool for the early directional breeding and industrial promotion of white Apostichopus japonicus.

The application discloses an antioxidant peptide from Apostichopus japonicus and a preparation method and application thereof, and relates to the technical field of biological medicines. The antioxidant peptide from Apostichopus japonicus has an amino acid sequence as shown in SEQ ID NO. 1. The antioxidant peptide with the amino acid sequence of LPPF is screened from the Apostichopus japonicus, and the peptide is verified to have excellent antioxidant activity and high safety, and has a good application prospect in the treatment of oxidative stress injury diseases.

The invention belongs to the technical field of enzymolysis feed preparation, and discloses a preparation method of alginic acid enzymolysis feed suitable for apostichopus japonicus. The method comprises the following steps: preparing an enzymolysis raw material from a basal feed and water in a specific proportion, and inoculating alginic acid degrading bacteria. According to the method, the basic feed is subjected to enzymolysis by using the crude enzyme of the alginic acid degrading bacteria, so that the palatability of the basic feed is improved, the nutrition and active components are improved, the diseases of the apostichopus japonicus are effectively prevented and treated, the breeding efficiency is improved, the intestinal flora structure is improved, and the growth, development and health of the apostichopus japonicus are promoted.

The application discloses a sea cucumber polysaccharide cancer inhibiting preparation and a preparation method and application thereof, and belongs to the technical field of biological medicine. The preparation is prepared from high-purity sea cucumber polysaccharide extracted from Apostichopus japonicus as a core active ingredient, and is matched with a folic acid modified polyethylene glycol-poly lactic acid copolymer targeting carrier and medicinal adjuvants, and is prepared through steps of standardization extraction and purification, targeting carrier preparation, granulation and coating. The application adopts an enzymolysis process combining with self-lyase and exogenous enzyme, and cooperates with multi-step purification and ultrafiltration grading technology to precisely control the purity and molecular weight of the polysaccharide, realizes active targeting of tumor cells through the FA-PEG-PLA targeting carrier, and improves the bioavailability through enteric coating technology. The preparation can up-regulate the expression of TNFR1 protein, activates the caspase cascade reaction to induce tumor cell apoptosis, has a significant inhibiting effect on solid tumors such as lung cancer, kidney cancer and liver cancer, and has very low toxicity to normal cells.

This invention discloses a sea cucumber farming device and method, comprising a base; a biomimetic reef body disposed on the base; an anti-siltation layer disposed on the base and surrounding the outside of the biomimetic reef body; and a liftable farming fence surrounding the anti-siltation layer, with a gap between them. The liftable farming fence includes several sets of movable net panels and a driving component. Each set of movable net panels includes multiple mesh plates, with adjacent mesh plates slidably connected to each other. The bottom of the mesh plate closest to the base is fixedly connected to the base, while the remaining mesh plates slide through the driving component. This invention provides a sea cucumber farming device. The liftable farming fence can adjust its height according to different growth stages of the sea cucumber, improving farming density and space utilization, and providing efficient feed supply. The anti-siltation layer, combined with tidal action, achieves self-cleaning, effectively removing silt, reducing the growth of harmful organisms, lowering the incidence of diseases, and improving the survival rate.

The present invention discloses an anti-aging composition and a preparation method thereof, belonging to the technical field of cosmetics. The anti-aging composition provided by the present invention comprises the following components in parts by mass: 0.001-0.01 part of freeze-dried powder of porcine umbilical cord mesenchymal stem cell secretions, 15-20 parts of extract of leaves of Argania spinosa, 10-13 parts of extract of Eucheuma muricatum, and 1-3 parts of extract of adventitious roots of Apostichopus japonicus. The anti-aging composition provided by the present invention has the effects of anti-aging and improving the migration rate of skin cells, and is overall mild and non-irritating. Applying the anti-aging composition of the present invention to cosmetics can effectively anti-aging, reduce wrinkles, improve skin elasticity and increase the water content of the stratum corneum. In addition, the components of the composition provided by the present invention are derived from natural sources, and the preparation method is simple, which is suitable for actual production.

The present invention discloses a chemical induction method for the triploid of Apostichopus japonicus Huachun No. 1, belonging to the technical field of genetic breeding of marine echinoderms. The chemical induction method comprises the following steps: promoting the maturation of broodstock, artificially inducing ovulation or sperm ejaculation, treating fertilized eggs with staurosporine, hatching the fertilized eggs to obtain juvenile sea cucumbers, and continuing to cultivate the juvenile sea cucumbers. The induction drug used in the present invention is staurosporine, which is a reversible inhibitor related to microfilament assembly and can inhibit the emission of the first polar body of fertilized eggs. Compared with traditional induction drugs such as cytochalasin B and 6-DMAP, staurosporine has the advantages of rapid inhibitory effect, low toxicity and easy cleaning. In addition, the staurosporine induction method provided by the present invention is completely different from the previous physical induction methods, not only reducing the operation difficulty, but also improving the survival rate of larvae and the triploid yield.

The invention provides a method for inhibiting vibrio splendidus virulence factors by using 5-iodoindole and application of the 5-iodoindole, and finds that 5-iodoindole with different concentrations can obviously reduce the expression of the virulence factors such as hemolytic activity and protease activity of vibrio splendidus on the premise of basically not influencing the growth of the vibrio splendidus. According to the method, the infection toxicity of vibrio splendidus can be weakened, so that the infection and morbidity probability of the host stichopus japonicus is reduced, and the infection symptom is relieved. Meanwhile, the invention further provides application of the 5-iodoindole in reducing expression of vibrio splendidus related virulence genes and improving the survival rate of host stichopus japonicus after vibrio splendidus challenge, and it is proved that the 5-iodoindole with the concentration of 20 [mu] M has guiding significance in actual stichopus japonicus production and breeding. And the vibrio splendidus virulence factor is researched, so that the infection and morbidity of the stichopus japonicus caused by the vibrio splendidus can be reduced.

The present application belongs to the technical field of molecular genetics, and relates to a genetic sex molecular marker of Apostichopus japonicus, a primer, a method, a kit and application. The nucleotide sequence of the molecular marker is shown in the sequence table SEQ ID NO. 1. The Apostichopus japonicus with the molecular marker is male. The present application also provides a primer for amplifying the molecular marker, a loop-mediated isothermal amplification detection primer group and reagent or kit, an identification method and the like. The molecular marker and the developed kit can be used to carry out sex identification of Apostichopus japonicus, and solve the problem of accurate identification of male and female in Apostichopus japonicus breeding.

The present invention relates to an Apostichopus japonicus collagen peptide and its application. The amino acid sequence of the Apostichopus japonicus collagen peptide is Asp-Cys-Asp-Pro-Arg-Leu (DCDPRL), which is an Apostichopus japonicus collagen peptide with both antioxidant and hypoglycemic activities. The present invention combines bioinformatics and molecular docking techniques to virtually screen novel bifunctional bioactive peptides with antioxidant and hypoglycemic activities from the collagen of Apostichopus japonicus. The Apostichopus japonicus collagen peptide DCDPRL screened in the present invention has the advantages of strong antioxidant ability and good hypoglycemic activity. The research on multi-target bioactive peptides carried out in the present invention can be applied to fields such as dietary nutrition and biomedical products.

The present invention belongs to the field of food biotechnology, and particularly relates to a sea cucumber-derived antioxidant peptide, a preparation method thereof, and an application thereof. The sea cucumber-derived antioxidant peptide of the present invention is prepared and screened from Apostichopus japonicus, and includes at least one of the peptides shown in SEQ.ID.NO.1-4 in terms of amino acid sequence, and the docking energies with the protein receptor Keap1 are -8.6 kcal / mol, -9.4 kcal / mol, -9.1 kcal / mol, and -8.8 kcal / mol respectively. The obtained antioxidant peptide of the present invention can be applied to foods, cosmetics or pharmaceuticals, and is of great significance for the development of new antioxidant products.

The application provides a kind of umami peptide derived from Apostichopus japonicus, amino acid sequence is any one of Glu-Gly-Ala-Thr (EGAT), Gly-Ala-Asp-Ala (GADA), Asp-Ser-Ala-Thr (DSAT), Asp-Ser-Thr-Thr (DSTT) or Ser-Ser-Ala-Glu (SSAE).The application also provides the preparation method, identification method and application of the umami peptide.The application adopts step-by-step enzymatic hydrolysis method, through raw material pretreatment, Alcalase enzyme first-step enzymatic hydrolysis, flavourzyme second-step enzymatic hydrolysis, sterilization centrifugation, ultrafiltration separation, gel chromatography separation and purification steps, to obtain umami peptide;through the integration identification strategy of "electronic tongue preliminary screening-peptide sequence identification-virtual screening-molecular docking simulation-electronic tongue taste verification", five tetrapeptides with umami activity EGAT, GADA, DSAT, DSTT and SAEA are identified from Apostichopus japonicus protease hydrolysate for the first time, which have small molecular weight, confirmed to have pure umami, and weak bitter taste, sour taste and other undesirable flavors;the umami peptide can be used for preparing food condiment, nutritional enhancer or flavor enhancer;belongs to the field of food science and bioactive peptide technology.

The invention discloses a microsatellite molecular marker for genetic structure and germplasm identification of apostichopus japonicus, and belongs to the technical field of molecular markers. The microsatellite molecular marker has any one or more nucleotide sequences as shown in SEQ ID NO. 1 to SEQ ID NO. 12. The microsatellite marker primers are respectively designed aiming at the 12 microsatellite sequences, the microsatellite marker primers are high in specificity, stable in amplification and high in polymorphism, and can be used for genetic structure analysis, germplasm resource identification and genetic relationship identification of apostichopus japonicus populations, and a powerful tool is provided for genetic breeding of apostichopus japonicus.

The invention discloses a microsatellite marker related to the early body color of apostichopus japonicus and application of the microsatellite marker, and belongs to the technical field of molecular markers. Two microsatellite sequences are obtained based on apostichopus japonicus genome screening and are numbered as AJ732 and AJ812 respectively, and the sequences of the two microsatellite sequences are shown as SEQ ID NO.1 and SEQ ID NO.2 in sequence. The microsatellite marker primers are respectively designed for the two microsatellite sequences, and the microsatellite marker primers are high in specificity and stable in amplification, can be used for judging the body color characters of the apostichopus japonicus larvae, can improve the breeding efficiency and accuracy of apostichopus japonicus population, and provides a powerful tool for early-stage directional breeding and industrial popularization of the apostichopus japonicus.

This invention discloses a method for predicting the spawning period of *Stichopus japonicus*, belonging to the field of *Stichopus japonicus* aquaculture data prediction. This invention analyzes the correlation between body weight, accumulated temperature, and changes in total saponin content in the gonads of female *Stichopus japonicus* during a period before and after spawning, constructs a model of the relationship between changes in total saponin content in the gonads and the timing of egg release, and establishes a method for accurately predicting the spawning timing of *Stichopus japonicus*. Using this prediction method, the spawning timing of *Stichopus japonicus* can be accurately determined, solving the problem of not being able to accurately predict the spawning timing for timely harvesting. Producers can then plan the water body used for seedling cultivation in their workshops.

The invention belongs to the technical field of molecular genetics, and relates to an apostichopus japonicus genetic sex molecular marker, a primer, a method, a kit and application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table. The apostichopus japonicus with the molecular marker is male. The invention also provides a primer for amplifying the molecular marker, a loop-mediated isothermal amplification detection primer group, a reagent or a kit, an identification method and the like. By utilizing the molecular marker and the developed kit, the sex identification of the apostichopus japonicus can be carried out, and the problem of accurate male and female identification in the culture of the apostichopus japonicus is solved.

The invention discloses lactococcus lactis GL-1 and application of the lactococcus lactis GL-1 in stichopus japonicus culture, and belongs to the technical field of aquatic product beneficial microorganisms and application of the aquatic product beneficial microorganisms. The lactococcus lactis GL-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 16, 2025, the preservation number is CGMCC No.35938, and the lactococcus lactis GL-1 is separated from the traditional fermented food thick broad-bean sauce. The strain can well adapt to different environments and can be used for preparing stichopus japonicus feed additives, and when the viable count of lactococcus lactis GL-1 in feed is larger than or equal to 1 * 10 < 8 > CFU / g, the low-salt stress resistance of stichopus japonicus can be remarkably improved, and the survival rate and the growth performance can be improved; meanwhile, the intestinal flora structure is improved, beneficial bacterium colonization is promoted, immune-related pathways such as a Wnt signal pathway are activated, and the health level of the stichopus japonicus is improved from multiple levels. A safe and effective microbial solution is provided for solving the problem of low salt stress in stichopus japonicus culture.