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Method for identifying apostichopus japonicus by means of specific peptide fragment group

A sea cucumber and peptide technology, applied in the biological field, can solve the problems of lack of technical level and deceiving consumers.

Active Publication Date: 2016-10-12
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the lack of a particularly effective method of identifying sea cucumber species at present, the method of identifying sea cucumber species is only based on traditional appearance observation, lacking technical support, resulting in many unscrupulous merchants cheating consumers by shoddy goods and false ones. Therefore, it is necessary to An efficient and accurate method for identifying sea cucumbers

Method used

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  • Method for identifying apostichopus japonicus by means of specific peptide fragment group
  • Method for identifying apostichopus japonicus by means of specific peptide fragment group
  • Method for identifying apostichopus japonicus by means of specific peptide fragment group

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0133] Example 1. Sequence source and alignment information of specific polypeptides

[0134] 1. The unique polypeptides of the sea cucumber are derived from the protein Arginine Kinase [Apostichopusjaponicus], and its Accesion number on NCBI is gi|4586462. After mass spectrometry analysis, the polypeptide SEQ ID NO. 1: GTSGEFTESVGGVYDISNLDR; SEQ ID NO. 2: GESIDDLVPK; SEQ ID NO. 3: DDFPNFEGHK; SEQ ID NO. 4: MVSEALNSLAADLK; SEQ ID NO. 5: IISMQMGGNMK; SEQ ID NO. 6: ILDDVLDPAYVISSR ; SEQ ID NO. 7: HFTSGGMAR; SEQ ID NO. 8: TQQQLIDDHFLFDRPVSR does exist in sea cucumbers, with good peak shape and high intensity.

[0135] figure 1 It is the chromatogram of the polypeptide GTSGEFTESVGGVYDISNLDR in sea cucumber, and its m / z is 1102.0.

[0136] figure 2 It is the chromatogram of the peptide GESIDDLVPK in sea cucumber, with m / z of 536.8.

[0137] image 3 It is the chromatogram of the polypeptide DDFPNFEGHK in sea cucumber, and its m / z is 603.3.

[0138] Figure 4 It is the chromatogram of the...

Embodiment 2

[0144] Example 2. Sequence source and alignment information of specific polypeptides

[0145] 1. The unique polypeptides of the sea cucumber are all derived from the protein major yolk protein 1 [Apostichopusjaponicus], and its Accesion number on NCBI is gi|240846031. After mass spectrometry analysis, the polypeptide SEQ ID NO. 9: AATTADDNTVIGNLLK; SEQ ID NO. 10: DFLLQPIMMQDPVMR; SEQ ID NO. 11: DYDSEYTVPVISR; SEQ ID NO. 12: EEITEMLTTILSER; SEQ ID NO. 13: IGSVFESVSR; SEQ ID NO. 14: QEITELLTTILEER ; SEQ ID NO.15: SFEAIKPSTFDWWK; SEQ ID NO.16: SVPNFHLPIGHLLSNGVIPR; SEQ ID NO.17: TGDAFVSLR; SEQ ID NO.18: VDEFTGIVGSLR; SEQ ID NO.19: YLNPIWLSPTFK does exist in sea cucumbers, and the peak shape is good ,high strength.

[0146] Picture 9 It is the chromatogram of the polypeptide AATTADDNTVIGNLLK in sea cucumbers, and its m / z is 837.4.

[0147] Picture 10 It is the chromatogram of the polypeptide DFLLQPIMMQDPVMR in sea cucumbers, and its m / z is 917.5.

[0148] Picture 11 It is the chromat...

Embodiment 3

[0158] Example 3. Sequence source and alignment information of specific polypeptides

[0159] 1. The above-mentioned unique peptides of sea cucumber are all derived from the protein major yolk protein 2 [Apostichopusjaponicus], and its Accesion number on NCBI is gi|240846033. After mass spectrometry analysis, the polypeptide SEQ ID NO. 20: ETDPNTIIGNLLMDYDSEYTVPLITK; SEQ ID NO. 21: IFSDAQNNEDQYHIFGSASIFAPETVK; SEQ ID NO. 22: MYPVPLNMQTHPTYLGSR; SEQ ID NO. 23: QGVGNSHEPFYGNEGALR; SEQ ID NO. 24: VDEFTPVGSLR; SEQ ID NO. 26: VISVGTTENK; SEQ ID NO. 27: WLTPTYPEFLDTMK does exist in sea cucumbers, with good peak shape and high intensity.

[0160] Picture 20 It is the chromatogram of the polypeptide ETDPNTIIGNLLMDYDSEYTVPLITK in sea cucumbers, and its m / z is 985.8.

[0161] Figure 21 It is the chromatogram of the polypeptide IFSDAQNNEDQYHIFGSASIFAPETVK in sea cucumber, and its m / z is 1010.1.

[0162] Figure 22 It is the chromatogram of the polypeptide MYPVPLNMQTHPTYLGSR in sea cucumber, a...

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Abstract

The invention relates to a method for identifying apostichopus japonicus by means of a specific peptide fragment group, and a group of marker peptides capable of being used for identifying apostichopus japonicus when used alone or otherwise. The marker peptides are low-homology polypeptide fragments of apostichopus japonicus protein, wherein the low-homology polypeptide fragments are exclusive to apostichopus japonicus with a length of 5-30 amino acids. Even though it is found that there are plants or bacteria having the same protein, the marker peptides are exclusive to apostichopus japonicus by comparison with thelenota ananas, acaudina molpadioidea, isostichopus badionotus and holothuria Mexicana.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method for identifying sea cucumber (Apostichopus japonicus) by using a specific peptide group. Background technique [0002] Sea cucumbers are rich in acid mucopolysaccharides, which have immunomodulators, anticoagulant, blood sugar control and blood lipid lowering effects on the human body. Therefore, sea cucumber enjoys the reputation of "nutrition treasure house", and modern people regard sea cucumber as the "first of the eight treasures of seafood". There are many types of Shanghai ginseng in the market. Different sea cucumbers have huge price differences due to their nutritional value and taste. Sea cucumber is a kind of sea cucumber. It is the best quality among more than 20 kinds of edible sea cucumbers in China. In the sea cucumber family, the better quality is the sea cucumber from Shandong Peninsula and Liaodong Peninsula. According to the "Supplements from Comp...

Claims

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Application Information

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IPC IPC(8): C07K14/435G01N27/62G01N1/28
CPCG01N1/28G01N33/6848C07K14/43504
Inventor 张鸿伟张晓梅鲍蕾梁成珠徐彪
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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