The invention discloses vaccinium bracteatum fruit-containing sea hare seafood soup base with a function of supplementing the kidney to control nocturnal emission, and a preparation method of the soup base. The Vaccinium bracteatum fruit-containing sea-hare seafood soup base is characterized by being prepared from the following raw materials in parts by weight: Vaccinium bracteatum fruit, juglantis septum, thelenota ananas, radix morindae officinalis, turtle shell, fructus schisandrae, black sesame, syngnathus, sea hare meat meal, undaria pinnatifida granules, dried nori, onion granules, shallot flakes, cassava starch, table salt, ground pepper, ginger and anise; the soup base is prepared by the processes of cleaning, drying, mixing, removing impurities, sterilizing, bagging and the like. The vaccinium bracteatum fruit-containing sea hare seafood soup base is delicious in taste and convenient to store, has the effect of supplementing the kidney to control nocturnal emission, and is simpler and healthier in preparation technology.