50 results about "Thelenota ananas" patented technology

The invention relates to a method for identifying thelenota ananas by means of a special peptide fragment group, and provides a group of contrast polypeptide used for identifying the thelenota ananas through mass-spectrography. The sequences of the polypeptide are shown as SEQ ID NO.1:ADIAEDSLK, SEQ ID NO.2:AGPAGDDAPR, SEQ ID NO.3:MPIPLLEGK and SEQ ID NO.4:LDIPLLEGK. Through identification, the peptide fragment of which the sequence is shown as the SEQ ID NO.1 ADIAEDSLK is the special peptide fragment of the thelenota ananas and is not expressed in other species; the SEQ ID NO.2:AGPAGDDAPR, SEQ ID NO.3:MPIPLLEGK and SEQ ID NO.4:LDIPLLEGK exist in the other species, but are not expressed in stichopus japonicus, acaudina molpadioidea, isostichopus badionotus and isostichopus fuscus; the peptide fragments are the special peptide fragments of the thelenota ananas.

The invention belongs to the technical field of food processing and in particular relates to a fast-puffed holothurian and a preparation method thereof. The method comprises the following steps: snipping a fresh holothurian at a position with the distance from the belly to one-third part of the anus of the fresh holothurian, heating the fresh holothurian for 15-25 minutes in hot water being 96-100 DEG after taking out the viscera, taking out and cleaning the treated holothurian for standby; placing the obtained holothurian in a high-pressure steam pot, heating for 3-8 minutes under the temperature being 105-115 DEG C, and drying under low temperature to obtain products. The holothurian is one selected from Dalian holothurian, cucumaria frondosa gunnerus and thelenota ananas; the low-temperature drying refers to vacuum freeze drying or low-temperature forced air drying. Before being eaten, the fast-puffed holothurian prepared by using the method disclosed by the invention is placed in a vacuum cup for puffing, and then can be eaten. The puffing of the puffed holothurian is soft, after rehydration, various sense organ qualities of the puffed holothurian are good, the carrying and the eating are convenient, and the function of nourishing in real time can be realized. The fast puffed holothurian is a novel holothurian product and makes up the blank in the market.

The invention discloses a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method for identifying seven sea cucumber species. The PCR-RFLP method includes the steps of firstly extracting DNA (deoxyribonucleic acid) modules of sea cucumbers; subjecting specificity fragment of a CO I (coenzyme I) gene to PCR amplification to obtain a fragment about 690bp; secondarily subjecting amplified product to restriction enzyme digestion; and finally subjecting the enzyme-digested product to electrophoresis detection. Different sea cucumbers obtain stripes in different numbers and sizes, and selected specificity incision enzymes respectively include: Californian paracanthurus cucumber: SSP I, North Atlantic Ocean cucumaria: EcoR V, apostichopus japonicas: SSP I, thelenota ananas: EcoR V, acaudina molpadioidea: Sca I, isostichopusbadionotus: Pvu II, and actinopyga mauritiana: EcoR V and SSP I. The PCR-RFLP method overcomes the defects that a conventional morphological identification method is prone to affecting in accuracy, is heavy in workload and long in consumption time and the like. The PCR-RFLP method can be completed by only one-time PCR reaction, one-time enzyme digestion reaction and one-time polypropylene gel electrophoresis, and is simple to operate, short in parting time and good in specificity.

The invention relates to a multiple PCR method for rapidly identifying four varieties of sea cucumbers and belongs to the field of molecular biology. The method adopts mitochondria CO I genes of four varieties of sea cucumbers as target DNA, and by sequence comparison, species specificity primers of the four varieties of sea cucumbers are designed respectively. The CO I genes of the sea cucumbers are extracted, multiple-PCR detection is carried out, thus judging the varieties of the sea cucumbers according to the molecular weight of PCR products. After the multiple-PCR detection is carried out on apostichopus japonicus, thelenota ananas, California apostichopus japonicus and Iceland sea cucumbers, the molecular weights of characteristic electrophoretic bands are 202bp, 301bp, 261bp and 358bp sequentially. The identification method has simple operation, low cost, short inspection period, good specificity and repeatability and high sensitivity, overcomes the defects of large workload, long consumed time, high cost and the like of the traditional sea cucumber morphology identification method and can realize rapid and accurate identification of the varieties of the sea cucumbers.

The invention discloses a health-care food which comprises components in parts by weight as follows: 40-60 parts of taro meal, 4-8 parts of lemon grass powder, 1-2 parts of leonurus japonicas powder, 3-7 parts of thelenota ananas, 10-20 parts of finger citron, 1-5 parts of lotus leaf extracts, 5-20 parts of garlic extracts, 1-3 parts of broccoli extracts, 1-3 parts of horny goat weed extracts and 1-3 parts of microcrystalline cellulose. The health-care food is made by main auxiliary raw material of taro meal, lemon grass, leonurus japonicas, thelenota ananas, finger citron, lotus leaf extracts, garlic extracts, broccoli extracts, horny goat weed extracts and microcrystalline cellulose, contains a large amount of vitamin C, has the efficacy of invigorating stomach, aiding digestion, adjusting immunity of the organism and relieving fatigue, has no side effect, and is green, safe and suitable for conditioning a chronic gastric disease and relieving stomach discomfort.

The invention relates to a method for identifying apostichopus japonicus by means of a specific peptide fragment group, and a group of marker peptides capable of being used for identifying apostichopus japonicus when used alone or otherwise. The marker peptides are low-homology polypeptide fragments of apostichopus japonicus protein, wherein the low-homology polypeptide fragments are exclusive to apostichopus japonicus with a length of 5-30 amino acids. Even though it is found that there are plants or bacteria having the same protein, the marker peptides are exclusive to apostichopus japonicus by comparison with thelenota ananas, acaudina molpadioidea, isostichopus badionotus and holothuria Mexicana.

The invention discloses a Chinese medicinal composition for treating insomnia due to blood stasis. The Chinese medicinal composition comprises the following Chinese medicinal herbs: American ginseng, concha margaritifera, tuber fleeceflower stems, glossy privet fruits, black lotus seeds, heterophylly faalsestarwort roots, dongkey-hide galatin, tortoise plastron, common anemarrhena rhizome, deerhorn glue, largehead atractylodes rhizome, poria, rhizoma dioscoreae, dried longan pulp, ladybell roots, swimming crabs, axillary choerospondias fruits, tree peony root bark, cape jasmine fruits, common burreed rhizome, zedoary rhizome, spina date seeds, white paeony roots, herb of nutgrass galingale, largeflower jasmine flowers, epiphyllum oxypetalumca, szechuan lovage rhizome, fructus aurantii, thelenota ananas, platycodon roots, peach seeds, unprepared rehmannia roots, red paeony roots, medicinal cyathula roots, stigma croci, radix ophiopogonis, dendrobium, milkvetch roots, largehead atractylodes rhizome, largetrifoliolious bugbane rhizmome, radix codonopsis, Chinese angelica, radix bupleuri and radix glycyrrhizae preparata. Clinical experiments show that the Chinese medicinal composition can be used for safely and effectively treating insomnia due to blood stasis.

The invention discloses a method for hybridization of a south thelenota ananas and a north stichopus japonicus. The method is characterized by comprising the steps of seed sorting, water temperature adjustment, group pouring in a pool, incubation and management after the incubation. The method solves the problem that the conventional breeding method for self-breeding of sea cucumbers cannot produce novel sea cucumber varieties with the advantages of the south thelenota ananas and the north stichopus japonicus, and is suitable for breeding the sea cucumbers in each sea area and coastal areas.

The invention relates to a real-time fluorescence PCR detection method of Thelenota ananas; and moreover, primers and a probe with species specificity are designed on basis of a highly species-specific Thelenota ananas mitochondrial cytochrome oxidase subunit I sequence (GenBank: FJ589205.1) so as to realize species identification on the Thelenota ananas by adopting a Real-Time PCR method. Owe torelatively high specificity and sensitivity of the primers and the probe, the real-time fluorescence PCR detection method of the Thelenota ananas can be used for identifying authenticity of deep processed sea cucumber products.

The invention relates to a thelenota-ananas total smilax-saponins composition extracted from the thelenota-ananas, belonging to the technical field of medicines. The invention also provides the preparation method and the usage of the total saponins. The thelenota-ananas extraction has the advantages of low cost, good controllability and stable quality.

The invention relates to a novel health care food, which is prepared from the following ingredients in parts by weight: 30-50 parts of taro powder, 4-9 parts of sweet potato, 2-7 parts of lemon grass powder, 1-2 parts of herba leonuri powder, 3-7 parts of thelenota ananas, 15-23 parts of citrus chirocarpus, 4-10 parts of red date, 2-4 parts of lotus leaf extract, 3-5 parts of honey, 5-20 parts of garlic extract, 1-3 parts of broccoli extract, 2-12 parts of chestnut, 1-4 parts of herba epimedii extract, 1-5 parts of microcrystalline cellulose and 2-10 parts of semen boitae. The novel health care food has the advantages that the formula of the health care food contains a great quantity of vitamin C, has the efficacies on invigorating stomach, aiding digestion, regulating immunity of the organism and alleviating fatigue, is free from a toxic or side effect, is green and safe and is suitable for conditioning chronic gastric diseases and alleviating stomach discomfort.

The present invention discloses a method for determining oligomerization thelenota ananas glycosaminoglycan content. The method is characterized by comprising: (1) respectively preparing an oligomerization thelenota ananas glycosaminoglycan sample solution and a fengshensu control sample solution; (2) adopting high performance liquid chromatography to analyze the oligomerization thelenota ananas glycosaminoglycan sample solution, wherein the chromatography conditions comprise that: the Shodex OhpakSB-804HQ type gel chromatography column is adopted, the mobile phase is 0.08-0.12 mol / L NaCl, the column temperature is 34-36 DEG C, the flow rate is 0.3-0.7 ml / min, the detection wavelength is 206+ / -2 nm, and the injection volume is 20-30 mul; and (3) calculating the oligomerization thelenota ananas glycosaminoglycan content according to the high performance liquid chromatography analysis result.

A general saponin of Thelenota ananas, its preparing process, and its application in medicine field are disclosed.

The invention relates to a health care medicine for treating spleen and stomach deficiency-cold type chronic gastritis. The health care medicine is prepared from the following raw medicines in parts by weight: 9 parts of peucedanum morisonii bess, 6 parts of pleurospermum hookeri, 9 parts of angelica niyida wolff, 10 parts of winged yam rhizome, 50 parts of rose, 6 parts of thelenota ananas, 15g of Chinese dates, 3 parts of cassia twig and 1.5 parts of liquorice. Practices prove that the health care medicine disclosed by the invention can be safely and effectively used for treating the spleen and stomach deficiency-cold type chronic gastritis. The health care medicine disclosed by the invention is prepared from medicinal materials which are sweet and light in taste and free of unpleasant smell, and can be drunk for a long time with good acceptance degree.

The invention belongs to the technical field of food processing and in particular relates to a fast-puffed holothurian and a preparation method thereof. The method comprises the following steps: snipping a fresh holothurian at a position with the distance from the belly to one-third part of the anus of the fresh holothurian, heating the fresh holothurian for 15-25 minutes in hot water being 96-100 DEG after taking out the viscera, taking out and cleaning the treated holothurian for standby; placing the obtained holothurian in a high-pressure steam pot, heating for 3-8 minutes under the temperature being 105-115 DEG C, and drying under low temperature to obtain products. The holothurian is one selected from Dalian holothurian, cucumaria frondosa gunnerus and thelenota ananas; the low-temperature drying refers to vacuum freeze drying or low-temperature forced air drying. Before being eaten, the fast-puffed holothurian prepared by using the method disclosed by the invention is placed in a vacuum cup for puffing, and then can be eaten. The puffing of the puffed holothurian is soft, after rehydration, various sense organ qualities of the puffed holothurian are good, the carrying and the eating are convenient, and the function of nourishing in real time can be realized. The fast puffed holothurian is a novel holothurian product and makes up the blank in the market.

The invention discloses an exclusive characteristic peptide segment and a method for identifying sea cucumber species by using the same. The exclusive characteristic peptide segment, namely a polypeptide marker, comprises particular characteristic peptide segments of sea cucumbers, apostichopus japonicus, California apostichopus japonicus, thelenota ananas, Atlantic cucumaria, holothuria atra andMexican ginsengs, and their sequences are shown in SEQ ID NO.1-13. According to the invention, the technology for identifying different sea cucumber species from at the polypeptide level is established, and the blank of sea cucumber species identification at home and abroad is filled.

The invention discloses a method for producing an instant asparagus product long in guarantee period. The method comprises the steps of cleaning asparagus, placing the asparagus in a blender to perform tumbling, soaking the asparagus in hot water at 85-95 DEG C, placing the asparagus into cold water to soak the asparagus, filtering the asparagus, drying the asparagus to cause the water content to be 35-45 wt%, and obtaining a first prefabricated material; adding water to a mixture of hawthorn, ficus carica, thelenota ananas and milk to perform decoction for 2-5 h, performing filtration, concentrating filtrate to cause the water content to be 15-35 wt%, and obtaining a second prefabricated material; placing the first prefabricated material in a boiled pot to perform braising for 20-35 min, adding salt, chicken essence and taro meal to perform uniform mixing and continuing to perform braising for 20-38 min, and obtaining a third prefabricated material; evenly mixing the second prefabricated material, the third prefabricated material, microcrystalline cellulose, calculus bovis extract, lotus leaf extract, yam flour, kiwi powder and ginkgo powder, performing bagging, performing vacuumizing and high temperature sterilization, and obtaining the instant asparagus product long in guarantee period.

The present invention relates to a method for identifying Prunus ginseng (Thelenota ananas) using a specific peptide group, and a set of control polypeptides for identifying Plum ginseng by mass spectrometry. The sequence of the polypeptide is: SEQ ID NO.1: ADIAEDSLK ; SEQ ID NO. 2: AGPAGDDAPR; SEQ ID NO. 3: MPIPLLEGK; SEQ ID NO. 4: LDIPLLEGK. It has been identified that the peptide described in SEQ ID NO.1: ADIAEDSLK is a proprietary peptide of Prunella ginseng, which has not been expressed in other species; SEQ ID NO.2: AGPAGDDAPR; SEQ ID NO.3: MPIPLLEGK; SEQ ID NO. 4: Although the peptides described in LDIPLLEGK exist in other species, they are not expressed in sea cucumbers, Haitian melons, American ginseng, Mexican ginseng, and American ginseng. The above peptides are exclusive peptides of plum blossom ginseng.

The invention belongs to the field of extracting natural active substances by a biological technology and particularly relates to a method for extracting glycosaminoglycan from thelenota ananas. The method comprises the following steps of Step (1) pretreating the thelenota ananas, Step (2) conducting alkaline hydrolysis on a body wall of the thelenota ananas, Step (3) conducting double-enzymolysis on the body wall of the thelenota ananas, Step (4) using trichloroacetic acid to remove protein in a double-enzymolysis solution after finishing the enzymolysis, Step (5) using ethanol to precipitate glycosaminoglycan of the thelenota ananas, Step (6) drying raw glycosaminoglycan of the thelenota ananas, Step (7) using hydrogen peroxide to decolorize, Step (8) using a cellulose ion-exchange column chromatographic method to purify glycosaminoglycan of the thelenota ananas, and Step (9) drying refined glycosaminoglycan of the thelenota ananas. The method can balance a relation between yield and purity of glycosaminoglycan of the thelenota ananas, and improves the purity of glycosaminoglycan of the thelenota ananas.

The invention belongs to the field of extraction of biotechnology natural active materials and particularly relates to a method for extracting and purifying GAGs from thelenota ananas. The method includes: step one, pre-processing thelenota ananas; step two, performing alkaline hydrolysis on the body wall of the thelenota ananas; step three, performing two-enzyme enzymolysis on the body wall of the thelenota ananas; step four, removing proteins in two-enzyme enzymatic hydrolysate through trichloroacetic acid after the enzymolysis; step five, precipitating the GAGs of the thelenota ananas through ethanol; step six, drying GAGs raw sugar of the thelenota ananas; step seven, purifying the GAGs of the thelenota ananas by using a cellulose ion exchange column chromatography and a gel column sequentially; and step eight, drying GAGs refined sugar of the thelenota ananas. According to the method, the relation between the yield and the purity of the GAGs of the thelenota ananas can be balanced, and the purity of the GAGs of the thelenota ananas can be improved.

The invention discloses vaccinium bracteatum fruit-containing sea hare seafood soup base with a function of supplementing the kidney to control nocturnal emission, and a preparation method of the soup base. The Vaccinium bracteatum fruit-containing sea-hare seafood soup base is characterized by being prepared from the following raw materials in parts by weight: Vaccinium bracteatum fruit, juglantis septum, thelenota ananas, radix morindae officinalis, turtle shell, fructus schisandrae, black sesame, syngnathus, sea hare meat meal, undaria pinnatifida granules, dried nori, onion granules, shallot flakes, cassava starch, table salt, ground pepper, ginger and anise; the soup base is prepared by the processes of cleaning, drying, mixing, removing impurities, sterilizing, bagging and the like. The vaccinium bracteatum fruit-containing sea hare seafood soup base is delicious in taste and convenient to store, has the effect of supplementing the kidney to control nocturnal emission, and is simpler and healthier in preparation technology.

The invention relates to the pharmaceutical technical field, in particular to a compound of thelenota ananas total saponins extracted from thelenota ananas. The invention further provides a preparation method and a use of the thelenota ananas total saponins. The compound has low cost of extract of the thelenota ananas, strong controllability and stable quality.

The invention relates to the field of medical technology, in particular to an anti-cancer compound Ananaside B which is separated from thelenota ananas, and the molecular formula is C54H87O26SNa. The chemical structure and the spatial configuration of the compound are determined by various modern spectral analysises, in particular to the comprehensive analysis by applying the advanced two-dimensional nuclear magnetic resonance spectra and high resolution mass spectra. In vitro anti-tumor trials show that the compound has significant inhibition function on A-549 lung cancer and IA-9 ovarian cancer and other 10 kinds of tumor cell strains. The compound can provide a lead compound for the development of new anti-tumor drugs, which has important values for the development and the utilization of the marine medicinal and biological resources of China.

The invention relates to the pharmaceutical technical field, particularly to an anti-cancer compound Ananaside C having a molecular formula of C41H63O17SNa and separated from thelenota ananas. The chemical structure and the spatial configuration of the compound are determined by various modern spectral analytic methods, particularly the comprehensive resolution of the advanced two-dimensional nuclear magnetic resonance spectroscopy and the high-resolution mass spectroscopy. A vitro anti-tumor experiment shows that the compound has the remarkable effect of inhibiting eight kinds of tumor cell strains such as A-549 lung caner, IA-9 ovary cancer and the like. The invention provides a precursor compound for developing a new anti-tumor pharmaceutical, and has the important value on the development and the utilization of the national marine medicinal organism resource.

The invention discloses a pipe fish kidney warming and yang invigorating sour soup base and a preparation method thereof. The soup base is prepared from the following raw materials in parts by weight: 45 to 55 parts of pipe fish, 20 to 30 parts of black-bone chicken, 20 to 30 parts of thelenota ananas, 15 to 20 parts of illicium verum, 15 to 20 parts of Chinese tuliptree bark, 15 to 20 parts of kidney of fur seal, 15 to 20 parts of cordyceps sinensis, 10 to 15 parts of deer's sinew, 10 to 15 parts of American ginseng, 10 to 15 parts of lucid ganoderma, 10 to 15 parts of dragon bone, 10 to 15 parts of cassia bark, 25 to 35 parts of wild tomato, 15 to 20 parts of spine date seed, 15 to 20 parts of raspberry, 15 to 20 parts of shine skin papaya, 15 to 20 parts of cornus officinalis, 10 to 15 parts of toosendan fruit, and 10 to 15 parts of rice wine. The preparation method comprises the following steps: preparing raw materials, extracting raw materials, carrying out centrifugation, mixing, preparing tomato paste, mixing, grinding, mixing all raw materials, concentrating, and drying. The provided soup base has a sour and fresh taste, is palatable, is rich in nutrients, and has the functions of warming the kidney, invigorating yang, dissipating binds, eliminating swelling, astringing, and inducing astringency, if people eat the soup base for a long term.

The invention discloses a honey peach sports drink, which is prepared from the following raw materials (by weight): 80-90 parts of fructose corn syrup, 24-25 parts of white granulated sugar, 12-15 parts of honey, 10-15 parts of compound juice, 0.6-0.8 part of salt, 0.1-0.2 part of a multi-vitamin preparation, 1-3 parts of lemon peel, 1-3 parts of pitaya peel, 1-2 parts of rose, 1-3 parts of honeysuckle flower, 1-2 parts of Gynura divaricata (Linn.) DC., a proper amount of water, 2-3 parts of coix seeds, 4-6 parts of sorghum rice, 1-3 parts of tartary buckwheat, 1-2 parts of male flowers of eucommia ulmoides oliv, 1.2-2 parts of Thelenota ananas, 1-3 parts of Angelica keiskei koidzmi and 1-2 parts of Nigella damascene.