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Exclusive characteristic peptide segment and method for identifying sea cucumber species by using same

A technology of sea cucumbers and species, which is applied in the field of specific characteristic peptides and its use to identify sea cucumber species, and can solve problems such as lack of technical level and deceiving consumers

Active Publication Date: 2019-08-09
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the lack of a particularly effective method of identifying sea cucumber species at present, the method of identifying sea cucumber species is only based on traditional appearance observation, lacking technical support, resulting in many unscrupulous merchants cheating consumers by shoddy goods and false ones. Therefore, it is necessary to An efficient and accurate method for identifying sea cucumbers

Method used

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  • Exclusive characteristic peptide segment and method for identifying sea cucumber species by using same
  • Exclusive characteristic peptide segment and method for identifying sea cucumber species by using same
  • Exclusive characteristic peptide segment and method for identifying sea cucumber species by using same

Examples

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Effect test

Embodiment 1

[0079] Example 1. Obtainment of specific characteristic peptides of 7 different sea cucumber species

[0080] This application uses the liquid mass analysis platform (UHPLC-HRMS and UHPLC-MS / MS) to conduct protein / peptideomics analysis on commercial dried sea cucumbers from 7 different ginseng species, and combine bioinformatics methods to analyze the generated mass spectrometry data Mining analysis was carried out, and 13 identifying signature peptides (ISPs) for the identification of 7 commercial sea cucumbers were screened out.

[0081] 1. Protein / peptide identification analysis based on DDA data

[0082] Obtained Through protein retrieval, a total of 34584 pieces of protein information from 374 sea cucumber species were obtained. Among them, Apostichopus japonicus is currently the ginseng species with the most information in the "Holothuroidea" (sea cucumber class) in the NCBI proteome database, with as many as 30,874 entries, while the second-ranked Atlantic melon ginsen...

Embodiment 2

[0124] Embodiment 2, sea cucumber sample processing and detection steps purchased in the market

[0125] The steps of analyzing the sea cucumber sample to be tested include:

[0126] (1) Sample pretreatment steps:

[0127] (1) Weigh 1g sea cucumber sample and homogenize it into a powder state, add 10mL protein extract (8M urea, 50mM NH 4 HCO 3 ) shake to extract protein, centrifuge at high speed and low temperature (20000r / min), take 200 μL of supernatant and transfer to 1ml EP tube to obtain protein solution;

[0128] (2) Add 2 μL of 1mol / L DTT to 100 μL of the above protein solution and react at 37°C for 1 hour;

[0129] (3) Add 10 μL of 1 mol / L IAA (protected from light during the preparation process) to the above reaction solution that has been cooled to room temperature, and react at room temperature for 1 hour in the dark;

[0130] (4) Use 10K filter membrane for 20000g ultrafiltration for 30 minutes, use 200μL 50mmol / L ammonium bicarbonate solution to repeatedly was...

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Abstract

The invention discloses an exclusive characteristic peptide segment and a method for identifying sea cucumber species by using the same. The exclusive characteristic peptide segment, namely a polypeptide marker, comprises particular characteristic peptide segments of sea cucumbers, apostichopus japonicus, California apostichopus japonicus, thelenota ananas, Atlantic cucumaria, holothuria atra andMexican ginsengs, and their sequences are shown in SEQ ID NO.1-13. According to the invention, the technology for identifying different sea cucumber species from at the polypeptide level is established, and the blank of sea cucumber species identification at home and abroad is filled.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a specific characteristic peptide segment and a method for identifying sea cucumber species using it. Background technique [0002] Sea cucumbers are rich in acidic mucopolysaccharides, which have the effects of immunomodulators, anticoagulants, blood sugar control and blood fat reduction on the human body. Therefore, sea cucumber enjoys the reputation of "treasure house of nutrition", and modern people regard sea cucumber as "the first of the eight delicacies of seafood". There are many kinds of sea cucumbers in the market, and the prices of different sea cucumbers vary greatly due to their different nutritional value and taste. Sea cucumber is a kind of sea cucumber, and it is the best quality among more than 20 kinds of edible sea cucumbers in China. Among the sea cucumber family, sea cucumbers from Shandong Peninsula and Liaodong Peninsula are of better quality. Acc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/34C12P21/06G01N33/68
CPCC07K14/43504C12P21/06G01N33/6848G01N2333/43504
Inventor 张鸿伟张晓梅赵雪徐杰
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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