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A kind of exclusive characteristic peptide segment and the method for identifying sea cucumber species using the same

A technology of sea cucumbers and species, which is applied in the field of specific characteristic peptides and its use to identify sea cucumber species, and can solve problems such as deceiving consumers and lack of technical level

Active Publication Date: 2022-07-05
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the lack of a particularly effective method of identifying sea cucumber species at present, the method of identifying sea cucumber species is only based on traditional appearance observation, lacking technical support, resulting in many unscrupulous merchants cheating consumers by shoddy goods and false ones. Therefore, it is necessary to An efficient and accurate method for identifying sea cucumbers

Method used

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  • A kind of exclusive characteristic peptide segment and the method for identifying sea cucumber species using the same
  • A kind of exclusive characteristic peptide segment and the method for identifying sea cucumber species using the same
  • A kind of exclusive characteristic peptide segment and the method for identifying sea cucumber species using the same

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Embodiment 1

[0079] Embodiment 1, the acquisition of the exclusive characteristic peptide of 7 kinds of different sea cucumber species

[0080] This application utilizes liquid mass analysis platforms (UHPLC-HRMS and UHPLC-MS / MS) to perform protein / peptidomics analysis of commercial dried sea cucumbers from 7 different ginseng species, combined with bioinformatics methods to generate mass spectrometry data. Mining analysis was carried out to screen out 13 identifying signature peptides (ISPs) for the identification of 7 commercial sea cucumbers.

[0081] 1. Protein / peptide identification and analysis based on DDA data

[0082] Through protein retrieval, a total of 34,584 pieces of protein information from 374 sea cucumber species were obtained. Among them, Apostichopus japonicus is currently the most informative species in the "Holothuroidea" (sea cucumber class) in the NCBI proteome database, with up to 30,874 entries, while the second-ranked Atlantic melon (Cucumaria frondosa) has only ...

Embodiment 2

[0124] Embodiment 2, the processing and detection steps of sea cucumber samples purchased in the market

[0125] The steps for analyzing the sea cucumber sample to be tested include:

[0126] (1) Sample preprocessing steps:

[0127] (1) Weigh 1 g of sea cucumber sample and homogenize it into powder, add 10 mL of protein extract (8M urea, 50mM NH) 4 HCO 3 ) shake to extract protein, centrifuge at high speed and low temperature (20000r / min), take 200 μL of supernatant and transfer it to 1ml EP tube to obtain protein solution;

[0128] (2) Take 2 μL of 1mol / L DTT and add it to 100 μL of the above protein solution to react at 37°C for 1 hour;

[0129] (3) Take 10 μL of 1 mol / L IAA prepared now (the configuration process needs to be protected from light), add it to the above-mentioned reaction solution that has been cooled to room temperature, and react in the dark at room temperature for 1 hour;

[0130] (4) 20000g ultrafiltration of 10K filter membrane was adopted for 30 minu...

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Abstract

The present application discloses an exclusive characteristic peptide segment and a method for identifying sea cucumber species using the same. The specific characteristic peptide segment, that is, the polypeptide marker, includes the unique characteristic peptide segment of Haitian melon, Apostichopus japonicus, Apostichopus japonicus, Plum ginseng, Atlantic melon, black sea cucumber, and Mexican ginseng; its sequence is as shown in SEQ ID NO. .1-13. The invention establishes a technology for identifying different sea cucumber species from the polypeptide level, and fills the gap in the identification of sea cucumber species at home and abroad.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an exclusive characteristic peptide segment and a method for identifying sea cucumber species with the same. Background technique [0002] Sea cucumber is rich in acidic mucopolysaccharides, which have the functions of immune regulator, anticoagulant, blood sugar control and blood lipid lowering on the human body. Therefore, sea cucumber enjoys the reputation of "nutrition treasure house", and modern people regard sea cucumber as "the first of the eight treasures of seafood". There are many kinds of sea cucumbers in the market, and the prices of different sea cucumbers vary greatly due to their different nutritional value and taste. Sea cucumber is a kind of sea cucumber, and it is the best quality among the more than 20 kinds of edible sea cucumbers in China. In the sea cucumber family, the best quality sea cucumbers are from Shandong Peninsula and Liaodong Peninsula. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K1/34C12P21/06G01N33/68
CPCC07K14/43504C12P21/06G01N33/6848G01N2333/43504
Inventor 张鸿伟张晓梅赵雪徐杰
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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