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Extraction method of holothuria atra glycosaminoglycan

An extraction method and glycosaminoglycan technology are applied in the field of extraction of black sea cucumber glycosaminoglycans, which can solve the problems of inability to inject and achieve the effect of improving the purity

Inactive Publication Date: 2013-04-10
QINGDAO MUNICIPAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Glycosaminoglycan is the main medicinal ingredient in sea cucumber related to stroke treatment; however, the black sea cucumber glycosaminoglycan extracted from black sea cucumber is a crude product and cannot be directly used to make injections

Method used

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Examples

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Effect test

Embodiment 1

[0013] The method for extracting glycosaminoglycans from black sea cucumbers in this embodiment includes the following steps: step 1: pretreatment of black sea cucumbers; step 2: alkaline hydrolysis of black sea cucumber body walls; step 3: double-enzyme hydrolysis of black sea cucumber body walls; Four: After the enzymatic hydrolysis is completed, remove the protein in the double-enzyme hydrolysis solution with trichloroacetic acid; Step five: Precipitate black sea cucumber glycosaminoglycans with ethanol; Step six: Dry black sea cucumber glycosaminoglycans; Step seven: Using cellulose ion exchange column chromatography and gel column to purify the black sea cucumber glycosaminoglycan; Step 8: drying the black sea cucumber glycosaminoglycan refined sugar. The specific method of each step is:

[0014] Step 1: Black sea cucumber pretreatment; quickly rinse the black sea cucumber with distilled water to remove the foreign matter adhered to the surface of the black sea cucumber, ...

Embodiment 2

[0027] The method for extracting glycosaminoglycans from black sea cucumbers in this embodiment includes the following steps: step 1: pretreatment of black sea cucumbers; step 2: alkaline hydrolysis of black sea cucumber body walls; step 3: double-enzyme hydrolysis of black sea cucumber body walls; Four: After the enzymatic hydrolysis is completed, remove the protein in the double-enzyme hydrolysis solution with trichloroacetic acid; Step five: Precipitate black sea cucumber glycosaminoglycans with ethanol; Step six: Dry black sea cucumber glycosaminoglycans; Step seven: Using cellulose ion exchange column chromatography and gel column to purify the black sea cucumber glycosaminoglycan; Step 8: drying the black sea cucumber glycosaminoglycan refined sugar. The specific method of each step is:

[0028] Step 1: Black sea cucumber pretreatment; quickly rinse the black sea cucumber with distilled water to remove the foreign matter adhered to the surface of the black sea cucumber, ...

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Abstract

The invention belongs to the field of natural active substance extraction by a biological technique, and particularly relates to an extraction method of holothuria atra glycosaminoglycan. The method comprises the following steps of 1, conducting pretreatment on holothuria atra, 2, conducting alkaline hydrolysis on a holothuria atra body wall, 3, conducting double-enzymolysis on the holothuria atra body wall, 4, removing protein from a double-enzymolysis solution with trichloroacetic acid after the enzymolysis is completed, 5, precipitating holothuria atra glycosaminoglycan with alcohol, 6, drying raw holothuria atra glycosaminoglycan, 7, purifying holothuria atra glycosaminoglycan by a cellulose ion exchange column chromatography and a gel column in sequence, and 8, drying refined holothuria atra glycosaminoglycan. The extraction method can balance a relation between yield and purity of holothuria atra glycosaminoglycan, and can improve the purity of holothuria atra glycosaminoglycan.

Description

technical field [0001] The invention belongs to the field of extraction of natural active substances in biotechnology, and in particular relates to an extraction method of black sea cucumber glycosaminoglycan. Background technique [0002] With the increase of population aging, cardiovascular and cerebrovascular diseases have become the biggest threat to human health. According to reports, cardiovascular and cerebrovascular diseases are one of the three major causes of death for the elderly. At present, the deaths due to cardiovascular and cerebrovascular diseases account for 34% of the total death toll, and about 3 / 4 of the surviving patients have different degrees of labor. Loss of ability and self-care ability. Ischemic stroke is the most common disease in cardiovascular and cerebrovascular diseases, accounting for 43-65% of cerebrovascular diseases. Human survival and quality of life. The world population-standardized incidence rate of stroke is 239.4 / 100,000, of whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 辛永宁林中华宣世英姜相君姜曼李雪洁
Owner QINGDAO MUNICIPAL HOSPITAL
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