The invention belongs to the technical field of biological
medicine manufacturing. The invention relates to a
collagen peptide, in particular to a
collagen peptide with functions of regulating
metabolism and losing weight and fat as well as a preparation method and application thereof. The preparation method of the
collagen peptide comprises the following steps: F1, washing fish scales, and crushing for later use; f2, mixing the fish scales with the
pyracantha fortuneana-soybean
peptide bacillus subtilis fermentation liquor, and decalcifying for 30-60 minutes under the condition that the pH (Potential of
Hydrogen) is 4.5-5.5, so as to obtain decalcified fish scales; f3, the decalcified fish scales and the
pyracantha fortuneana-soybean
peptide bacillus subtilis fermentation liquor are mixed, and enzymolysis is conducted for 1-6 h under the condition that the pH ranges from 7.0 to 8.0; and F4, centrifuging, taking supernate, carrying out
ultrafiltration, desalting, and freeze-
drying to obtain the collagen
peptide. According to the method disclosed by the invention, a
pyracantha fortuneana-soybean peptide
system is fermented by the
bacillus subtilis, so that an integrated process of decalcification, enzymolysis and
oxidation resistance of the collagen peptide is realized, and the whole process is more efficient, green and mild.
Functional verification shows that the FCPs prepared by the invention have potential effects of regulating
metabolism and assisting in losing weight, and the FCPs are combined with
orlistat to generate a synergistic effect.