Preparation method of a diglyceride oil with weight loss efficacy and application thereof

Abstract

The application belongs to the technical field of oil and fat preparation, and particularly relates to a preparation method of a glyceride oil with weight loss effect and application thereof. The method comprises the following steps: first, adding oil and fat into a basic nutrient solution, adding a bacterial strain for fermentation, filtering, and obtaining a fermentation product; then, adding glycerol into the fermentation product, adopting a lipase esterification reaction, and centrifugally separating to obtain an oil phase. The method adopts a dominant bacterial strain to degrade triglyceride, then adds glycerol, and adopts a dominant compound lipase to perform an esterification reaction, so as to obtain glyceride oil. The method can selectively generate 1,3-glyceride, and improve the proportion of the dominant oil and fat. Research shows that the glyceride oil prepared by the method has good weight loss and fat reduction effects, and has a good application prospect.

Description

Technical Field

[0001] This invention belongs to the field of oil preparation technology, specifically relating to a method for preparing diglyceride oil with weight-loss effects and its application. Background Technology

[0002] With changes in people's dietary structure, obesity has become an increasingly serious problem, and the development of functional oils has become a research hotspot in the food and health field.

[0003] Traditional edible oils (such as triglycerides, TAG) are easily hydrolyzed into free fatty acids and monoacylglycerols by lipases in the human body. After being absorbed through the intestines, they enter the bloodstream in the form of chylomicrons, and excessive intake can lead to fat accumulation. Diacylglycerol (DAG), as a natural oil component, is a fat molecule with only two fatty acid chains. Diacylglycerol can be used as an emulsifier, a fat shaping modifier, or as a base in food, pharmaceuticals, and cosmetics. Recent studies have found that diacylglycerol and triglycerides have different absorption and metabolic patterns. DAG preferentially forms monoacylglycerols and free fatty acids in the intestines and enters the liver directly through the portal vein for oxidation and energy supply, rather than being stored as chylomicrons. This characteristic gives it potential weight-loss benefits.

[0004] Currently, the main methods for industrial production of DAG include chemical transesterification, enzyme catalysis, and molecular distillation purification. Many patents in related fields have disclosed corresponding production methods, such as Chinese invention patent applications CN112513235A, CN116042736A, and CN105400837A. CN105400837A discloses an enzyme-catalyzed method for preparing diglycerides, comprising the following steps: (1) Partial hydrolysis of oil: Edible oil is partially hydrolyzed using an enzyme-catalyzed hydrolysis method, and after dehydration, a hydrolysate is obtained, such that the content of diglycerides in the hydrolysate is between 30.0 wt% and 35.0 wt%, and the content of free fatty acids is between 26.0 wt% and 30.0 wt%; (2) Re-esterification: Glycerol is added to the hydrolysate obtained in step (1) at 2.5 to 4 times its mass, and an enzyme-catalyzed esterification reaction is carried out to synthesize diglycerides. After removing excess glycerol, the edible diglycerides are obtained.

[0005] Chinese invention patent application CN116042736A discloses an enzymatic production process for diglycerides. The process involves the enzymatic hydrolysis of glycerol to prepare diglyceride oil. Specifically, under the catalysis of lipase, the oil and glycerol react to produce diglycerides. Homogenization of the reaction substrate before enzymatic hydrolysis improves the catalytic efficiency of lipase in the reaction system, thereby reducing reaction time, reducing enzyme dosage, increasing the diglyceride content in the product, and ultimately lowering production costs.

[0006] Existing technologies have been explored and researched to some extent in the preparation of diglycerides, achieving certain results. However, many processes are difficult to apply on a large scale, and the product yield needs further improvement. In addition, research shows that 1,3-DG has the best regulatory effect on the body, such as lowering blood lipids and weight loss. However, due to the lack of reaction specificity, most of the products obtained are mixtures of 1,2-DG and 1,3-DG, usually in a ratio of 7:3 to 6:4. Therefore, developing an efficient DAG preparation process while optimizing and increasing the content of 1,3-DG to enhance the weight loss effect and improve sensory properties has significant industrial value. Summary of the Invention

[0007] To overcome the above technical problems, the present invention provides a method for preparing a diglyceride oil with weight loss effect.

[0008] To achieve the above objectives, the technical solution provided by this invention is as follows:

[0009] A method for preparing a diglyceride oil with weight-loss effects includes the following steps:

[0010] (1) Add the oil to the basic nutrient solution, add the bacterial culture for fermentation, filter, and obtain the fermentation product;

[0011] (2) Add glycerol to the fermentation product, use lipase esterification reaction, and centrifuge to obtain the oil phase.

[0012] Preferably, the oil in step (1) is selected from one or more of the following: perilla seed oil, safflower seed oil, sea buckthorn seed oil, and Sichuan pepper seed oil.

[0013] Preferably, the basic nutrient solution in step (1) comprises the following components by weight percentage: 1-5% glucose, 0.1-10% peptone, 0.1-1% yeast extract, 0.001-0.01% sodium dihydrogen phosphate, 0.001-0.01% potassium dihydrogen phosphate, and the remainder is water.

[0014] Preferably, in step (1), after the strain is activated, it is inoculated into the basic nutrient solution at an inoculation amount of 5-10% v / v. The strain includes Rhodotorula buergerianum and Rhodotorula glutinis var. glutinis with an inoculation volume ratio of 1-5:1.

[0015] Preferably, the fermentation temperature in step (1) is 26-30℃ and the fermentation time is 24-72h.

[0016] Preferably, the lipase in step (2) includes Rhizopus niger lipase and Candida antarcticis lipase B in a mass ratio of 0.2-0.8:1, and the amount of lipase used is 1-10% of the mass of glycerol.

[0017] Preferably, the mass ratio of the fermentation product to glycerol in step (2) is 1:1-1.5.

[0018] Preferably, in step (2), the esterification reaction temperature is 40-60℃ and the reaction time is 5-10h.

[0019] Preferably, the oil mentioned in step (1) is selected from vegetable oil;

[0020] The vegetable oil is selected from one or more of the following: flaxseed oil, soybean oil, rapeseed oil, peanut oil, corn oil, sunflower seed oil, camellia seed oil, coconut oil, palm oil, palm kernel oil, olive oil, olive pomace oil, walnut oil, rice bran oil, rice bran oil, cottonseed oil, perilla seed oil, safflower seed oil, grape seed oil, tea seed oil, peony seed oil, sesame oil, wheat germ oil, maple seed oil, *Sapindus mukorossi* seed oil, sea buckthorn seed oil, DHA algal oil, medium-chain triglycerides, medium- and long-chain fatty acid edible oils, hemp seed oil, Sichuan pepper seed oil, Sichuan pepper oil, pumpkin seed oil, chili oil, almond oil, and sacha inchi oil.

[0021] Alternatively, the oil may be selected from animal oils.

[0022] Preferably, the oil mentioned in step (1) is selected from one or both of perilla seed oil and safflower seed oil.

[0023] The second objective of this invention is to provide the application of the diglyceride oil prepared by the above preparation method in the preparation of weight loss aid products.

[0024] Compared with the prior art, the technical advantages of the present invention are as follows:

[0025] (1) This invention uses a dominant microbial strain to ferment oil, degrading its triglycerides. Then, glycerol is added, and an esterification reaction is carried out under the action of the compound lipase of this invention to obtain selective diglyceride oil. This method can selectively generate 1,3-diglycerides, increasing the proportion of its dominant oil.

[0026] (2) Animal experiments have shown that the diglyceride oil prepared by this invention has good weight loss and fat reduction effects and has good application prospects. Detailed Implementation

[0027] The present invention will be described below through specific embodiments to make the technical solution of the present invention easier to understand and master, but the present invention is not limited thereto. Unless otherwise specified, the experimental methods described in the following embodiments are conventional methods; the reagents and materials described, unless otherwise specified, are all commercially available. The supplier of *Candida antarcticis* lipase B is Hangzhou Chuangke Biotechnology Co., Ltd., model number Lipozyme CALB. *Rhizopus oryzae* lipase, *Rhizopus oryzae* lipase (RML), product specification: 250 IUN / g, is supplied by Wuhan Tai'ao Technology Co., Ltd.

[0028] Example 1

[0029] The preparation method of diglyceride oil in this embodiment is as follows:

[0030] (1) Mix perilla seed oil and safflower seed oil at a mass ratio of 1:1, then add basic nutrient solution and mix. Then inoculate with Rhodotorula glutinis liquid and Rhodotorula glutinis var. glutinis liquid at an inoculation rate of 10% v / v (inoculation volume ratio of 2:1). Fermentation temperature is 28℃, fermentation time is 72h. After fermentation, sterilize at 121℃ for 20min and filter through a 0.45μm filter plate to obtain fermentation product.

[0031] The basic nutrient solution comprises the following components by weight percentage: 2.5% glucose, 1.5% peptone, 0.5% yeast extract, 0.002% sodium dihydrogen phosphate, 0.002% potassium dihydrogen phosphate, and the remainder is water.

[0032] Rhodotorula buergerianum (strain number: CICC32489, purchased from China Industrial Microbial Culture Collection Center) and Rhodotorula glutinis (strain number: CICC31774, purchased from China Industrial Microbial Culture Collection Center) were fermented and activated for 24 h in seed culture medium (by weight, the components were 5% glucose, 10% peptone, 1% yeast extract, 0.005% potassium dihydrogen phosphate, and the remainder was water) to obtain Rhodotorula buergerianum inoculum and Rhodotorula glutinis inoculum, respectively.

[0033] (2) Glycerol was added to the fermentation product at a mass ratio of 1:1, and 5% of the glycerol mass of *Rhizopus oryzae* lipase and *Candida antarcticis* lipase B at a mass ratio of 0.5:1 were added. The esterification reaction was carried out at 50℃ for 8 hours. After the reaction was completed, the mixture was centrifuged at 5500 rpm for 30 min to separate the oil and water. The oil phase was collected and its content was determined by high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD). The HPLC conditions were as follows: column: Waters C18 column (250 mm × 4.6 mm, 5 μm); mobile phase: acetonitrile-isopropanol (95:5 v / v), isocratic elution; flow rate: 1.0 mL / min; column temperature: 35℃. Evaporative light scattering detector conditions: ELSD drift tube temperature: 90℃; N2 flow rate: 2.2 L / min.

[0034] Example 2

[0035] The preparation method of diglyceride oil in this embodiment is as follows:

[0036] (1) Perilla seed oil and safflower seed oil were added to the basic nutrient solution at a mass ratio of 1:1 and then inoculated with Rhodotorula glutinis and Rhodotorula glutinis var. glutinis at an inoculation rate of 5% v / v (inoculation volume ratio of 5:1). The fermentation temperature was 28℃ and the fermentation time was 72h. After the fermentation was completed, the product was sterilized at 121℃ for 20min and filtered through a 0.45μm filter plate to obtain the fermentation product.

[0037] The basic nutrient solution comprises the following components by weight percentage: 2.5% glucose, 1.5% peptone, 0.5% yeast extract, 0.002% sodium dihydrogen phosphate, 0.002% potassium dihydrogen phosphate, and the remainder is water.

[0038] Rhodotorula buergerianum (strain number: CICC32489, purchased from China Industrial Microbial Culture Collection Center) and Rhodotorula glutinis (strain number: CICC31774, purchased from China Industrial Microbial Culture Collection Center) were fermented and activated for 24 h in seed culture medium (by weight, the components were 5% glucose, 10% peptone, 1% yeast extract, 0.005% potassium dihydrogen phosphate, and the remainder was water) to obtain Rhodotorula buergerianum inoculum and Rhodotorula glutinis inoculum, respectively.

[0039] (2) Add an equivalent amount of glycerol to the fermentation product, then add 2% of the glycerol by weight of *Rhizopus niger* lipase and *Candida antarcticis* lipase B at a mass ratio of 0.2:1. Esterification is carried out at 40℃ for 10 h. After the reaction, the mixture is centrifuged at 5500 rpm for 30 min to separate the oil and water. The oil phase is then used to determine its content using high-performance liquid chromatography-evaporative light scattering (HPLC-ELSD). HPLC conditions were: column: Waters C18 column (250 mm × 4.6 mm, 5 μm); mobile phase: acetonitrile-isopropanol (95:5 v / v), isocratic elution; flow rate: 1.0 mL / min; column temperature: 35℃. ELSD conditions: drift tube temperature: 90℃; N2 flow rate: 2.2 L / min.

[0040] Example 3

[0041] The preparation method of diglyceride in this embodiment is as follows:

[0042] (1) Mix perilla seed oil and safflower seed oil at a mass ratio of 1:1, then add basic nutrient solution and mix. Then inoculate with Rhodotorula glutinis and Rhodotorula glutinis var. glutinis at an inoculation rate of 10% v / v (inoculation volume ratio of 3:1). Fermentation temperature is 28℃, fermentation time is 72h. After fermentation, sterilize at 121℃ for 20min and filter through a 0.45μm filter plate to obtain fermentation product.

[0043] The basic nutrient solution comprises the following components by weight percentage: 2.5% glucose, 1.5% peptone, 0.5% yeast extract, 0.002% sodium dihydrogen phosphate, 0.002% potassium dihydrogen phosphate, and the remainder is water.

[0044] Rhodotorula buergerianum (strain number: CICC32489, purchased from China Industrial Microbial Culture Collection Center) and Rhodotorula glutinis (strain number: CICC31774, purchased from China Industrial Microbial Culture Collection Center) were fermented and activated for 24 h in seed culture medium (by weight, the components were 5% glucose, 10% peptone, 1% yeast extract, 0.005% potassium dihydrogen phosphate, and the remainder was water) to obtain Rhodotorula buergerianum inoculum and Rhodotorula glutinis inoculum, respectively.

[0045] (2) Add an equivalent amount of glycerol to the fermentation product, then add 8% of the glycerol by weight of *Rhizopus niger* lipase and *Candida antarcticis* lipase B at a mass ratio of 0.8:1. Esterification is carried out at 60℃ for 6 hours. After the reaction, the mixture is centrifuged at 5500 rpm for 30 minutes to separate the oil and water. The oil phase is then used to determine its content using high-performance liquid chromatography-evaporative light scattering (HPLC-ELSD). HPLC conditions were: column: Waters C18 column (250 mm × 4.6 mm, 5 μm); mobile phase: acetonitrile-isopropanol (95:5 v / v), isocratic elution; flow rate: 1.0 mL / min; column temperature: 35℃. ELSD conditions: drift tube temperature: 90℃; N2 flow rate: 2.2 L / min.

[0046] Comparative Example 1

[0047] The only difference between this comparative example and Example 1 is that the fermentation does not use Rhodotorula buergerianum, but instead uses Saccharomyces cerevisiae.

[0048] The preparation method of this comparative diglyceride oil is as follows:

[0049] (2) Mix perilla seed oil and safflower seed oil at a mass ratio of 1:1, then add basic nutrient solution and mix. Then inoculate with brewer's yeast liquid and Rhodotorula glutinis liquid at an inoculation rate of 10% v / v (inoculation volume ratio of 2:1). Ferment at 28℃ for 72 hours. After fermentation, sterilize at 121℃ for 20 minutes and filter through a 0.45μm filter plate to obtain the fermentation product.

[0050] The basic nutrient solution comprises the following components by weight percentage: 2.5% glucose, 1.5% peptone, 0.5% yeast extract, 0.002% sodium dihydrogen phosphate, 0.002% potassium dihydrogen phosphate, and the remainder is water.

[0051] Saccharomyces cerevisiae (strain number: CICC1002, purchased from China Industrial Microbial Culture Collection Center) and Rhodotorula glutinis (strain number: CICC31774, purchased from China Industrial Microbial Culture Collection Center) were fermented and activated for 24 h in seed culture medium (by weight, the components were 5% glucose, 10% peptone, 1% yeast extract, 0.005% potassium dihydrogen phosphate, and the remainder was water) to obtain Saccharomyces cerevisiae and Rhodotorula glutinis bacterial cultures, respectively.

[0052] (2) Add an equivalent amount of glycerol to the fermentation product, then add 5% of the glycerol by weight of *Rhizopus oryzae* lipase and *Candida antarcticis* lipase B at a mass ratio of 0.5:1. Esterification is carried out at 50℃ for 8 hours. After the reaction, the mixture is centrifuged at 5500 rpm for 30 minutes to separate the oil and water. The oil phase is then used to determine its content using high-performance liquid chromatography-evaporative light scattering (HPLC-ELSD). HPLC conditions: Column: Waters C18 column (250 mm × 4.6 mm, 5 μm); Mobile phase: acetonitrile-isopropanol (95:5 v / v), isocratic elution; Flow rate: 1.0 mL / min; Column temperature: 35℃. Evaporative light scattering detector conditions: ELSD drift tube temperature: 90℃; N2 flow rate: 2.2 L / min.

[0053] Comparative Example 2

[0054] The only difference between this comparative example and Example 1 is that the fermentation strain does not include the sticky red yeast strain.

[0055] The preparation method of this comparative diglyceride oil is as follows:

[0056] (3) Mix perilla seed oil and safflower seed oil in a mass ratio of 1:1, then add basic nutrient solution and mix. Then inoculate with Rhizopus spp. 10% v / v of the inoculation amount, ferment at 28℃ for 72 h. After fermentation, sterilize at 121℃ for 20 min and filter through a 0.45 μm filter plate to obtain the fermentation product.

[0057] The basic nutrient solution comprises the following components by weight percentage: 2.5% glucose, 1.5% peptone, 0.5% yeast extract, 0.002% sodium dihydrogen phosphate, 0.002% potassium dihydrogen phosphate, and the remainder is water.

[0058] Rhodotorula rubra (strain number: CICC32489, purchased from China Industrial Microbial Culture Collection Center) was fermented and activated for 24 h in seed culture medium (by weight, the components are 5% glucose, 10% peptone, 1% yeast extract, 0.005% potassium dihydrogen phosphate, and the remainder is water) to obtain Rhodotorula rubra culture liquid.

[0059] (2) Add an equivalent amount of glycerol to the fermentation product, then add 5% of the glycerol by weight of *Rhizopus oryzae* lipase and *Candida antarcticis* lipase B at a mass ratio of 0.5:1. Esterification is carried out at 50℃ for 8 hours. After the reaction, the mixture is centrifuged at 5500 rpm for 30 minutes to separate the oil and water. The oil phase is then used to determine its content using high-performance liquid chromatography-evaporative light scattering (HPLC-ELSD). The HPLC conditions were: column: Waters C18 column (250 mm × 4.6 mm, 5 μm); mobile phase: acetonitrile-isopropanol (95:5 v / v), isocratic elution; flow rate: 1.0 mL / min; column temperature: 35℃. The ELSD drift tube temperature was 90℃, and the N2 flow rate was 2.2 L / min.

[0060] Comparative Example 3

[0061] The only difference between this comparative example and Example 1 is that it does not use Candida antarcticis lipase B.

[0062] The preparation method of this comparative diglyceride oil is as follows:

[0063] (4) Mix perilla seed oil and safflower seed oil at a mass ratio of 1:1, then add basic nutrient solution and mix. Then inoculate with Rhodotorula glutinis liquid and Rhodotorula glutinis var. glutinis liquid at an inoculation rate of 10% v / v (inoculation volume ratio of 2:1). Ferment at 28℃ for 72h. After fermentation, sterilize at 121℃ for 20min and filter through a 0.45μm filter plate to obtain the fermentation product.

[0064] The basic nutrient solution comprises the following components by weight percentage: 2.5% glucose, 1.5% peptone, 0.5% yeast extract, 0.002% sodium dihydrogen phosphate, 0.002% potassium dihydrogen phosphate, and the remainder is water.

[0065] Rhodotorula buergerianum (strain number: CICC32489, purchased from China Industrial Microbial Culture Collection Center) and Rhodotorula glutinis (strain number: CICC31774, purchased from China Industrial Microbial Culture Collection Center) were fermented and activated for 24 h in seed culture medium (by weight, the components were 5% glucose, 10% peptone, 1% yeast extract, 0.005% potassium dihydrogen phosphate, and the remainder was water) to obtain Rhodotorula buergerianum inoculum and Rhodotorula glutinis inoculum, respectively.

[0066] (2) Add an equivalent amount of glycerol to the fermentation product, then add 5% (by weight) of Rhizopus oryzae lipase. Esterify at 50°C for 8 hours. After the reaction, centrifuge at 5500 rpm for 30 minutes to separate the oil and water. Take the oil phase and determine its content using high-performance liquid chromatography-evaporative light scattering (HPLC-ELSD). HPLC conditions: Column: Waters C18 column (250 mm × 4.6 mm, 5 μm); Mobile phase: acetonitrile-isopropanol (95:5 v / v), isocratic elution; Flow rate: 1.0 mL / min; Column temperature: 35°C. ELSD conditions: ELSD drift tube temperature: 90°C; N2 flow rate: 2.2 L / min.

[0067] Comparative Example 4

[0068] The only difference between this comparative example and Example 1 is that Rhizopus micranthum lipase is not used.

[0069] The preparation method of this comparative diglyceride oil is as follows:

[0070] (5) Mix perilla seed oil and safflower seed oil at a mass ratio of 1:1, then add basic nutrient solution and mix. Then inoculate with Rhodotorula glutinis liquid and Rhodotorula glutinis var. glutinis liquid at an inoculation rate of 10% v / v (inoculation volume ratio of 2:1). Ferment at 28℃ for 72h. After fermentation, sterilize at 121℃ for 20min and filter through a 0.45μm filter plate to obtain the fermentation product.

[0071] The basic nutrient solution comprises the following components by weight percentage: 2.5% glucose, 1.5% peptone, 0.5% yeast extract, 0.002% sodium dihydrogen phosphate, 0.002% potassium dihydrogen phosphate, and the remainder is water.

[0072] Rhodotorula buergerianum (strain number: CICC32489, purchased from China Industrial Microbial Culture Collection Center) and Rhodotorula glutinis (strain number: CICC31774, purchased from China Industrial Microbial Culture Collection Center) were fermented and activated for 24 h in seed culture medium (by weight, the components were 5% glucose, 10% peptone, 1% yeast extract, 0.005% potassium dihydrogen phosphate, and the remainder was water) to obtain Rhodotorula buergerianum inoculum and Rhodotorula glutinis inoculum, respectively.

[0073] (2) Add an equivalent amount of glycerol to the fermentation product and mix. Add 5% (by weight) of Candida antarctica lipase B. Esterify at 50°C for 8 hours. After the reaction, centrifuge at 5500 rpm for 30 minutes to separate the oil and water. Take the oil phase and determine its content using high-performance liquid chromatography-evaporative light scattering (HPLC-ELSD). HPLC conditions: Column: Waters C18 column (250 mm × 4.6 mm, 5 μm); Mobile phase: acetonitrile-isopropanol (95:5 v / v), isocratic elution; Flow rate: 1.0 mL / min; Column temperature: 35°C. ELSD conditions: ELSD drift tube temperature: 90°C; N2 flow rate: 2.2 L / min.

[0074] Experiment 1: The content of diglycerides prepared by different processes is shown in Table 1.

[0075] Table 1

[0076]

[0077] Experiment 2: Weight Loss Experiment

[0078] C57BL / 6J mice were divided into 9 groups of 10 mice each. One group was used as the control group (n=10 mice) and fed a normal diet. The remaining 8 experimental groups were divided into a model group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group. The remaining 8 groups were fed a high-fat diet (high-fat diet formula: 10% lard, 10% egg yolk powder, and 80% normal diet mixed together). After two weeks of experimentation, in addition to the corresponding diet, the experimental groups were also given 50 mg / kg of diglyceride oil prepared in the corresponding group once a day; the control group and the model group were given an equal volume of physiological saline. After 8 weeks, body weight was measured. Compared with the normal group, the body weight of the model group was significantly increased (P<0.05).

[0079] The body weight of mice in each group was measured to evaluate weight loss. Mice were euthanized, and their fat was collected to calculate the fat-to-body ratio. The results are shown in Tables 2 and 3.

[0080] Table 2

[0081]

[0082] Compared with the normal control group, **P<0.01; compared with the model control group, ##P<0.01; compared with the Example 1 group, &P<0.05.

[0083] Table 3

[0084]

[0085] Compared with the normal control group, **P<0.01; compared with the model control group, ##P<0.01; compared with the Example 1 group, &P<0.05.

[0086] Analysis of animal model body weight results showed that, compared with the blank control group, the body weight of rats in the model group was significantly increased ( P <0.01), indicating that obese rats were successfully induced. Compared with the model group, the body weight data of Examples 1-3 and Comparative Examples 1-4 were all lower than those of the model group. This indicates that the experimental groups inhibited the weight gain of mice to varying degrees. Compared with Comparative Examples 1-3, the fat / body weight ratio of Examples 1-3 was significantly lower (P<0.05), indicating that the fat / body weight ratio of Examples 1-3 was more effective in inhibiting fat growth.

[0087] The above detailed description is a specific description of one of the feasible embodiments of the present invention. This embodiment is not intended to limit the patent scope of the present invention. All equivalent implementations or modifications that do not depart from the present invention should be included within the scope of the technical solution of the present invention.

Claims

1. A method for preparing a diglyceride oil having a weight loss efficacy, characterized by, The method comprises the following steps: (1) adding oil into a basic nutrient solution, inoculating bacteria, filtering, and obtaining a fermentation product; (2) adding the fermentation product into glycerol, performing esterification reaction by using lipase, and centrifugally separating to obtain the product; In step (1), the bacteria are Rhodosporidium fluviale and Rhodotorula mucilaginosa var. glutinis with a volume ratio of 1-5:

1. In step (2), the lipase is Rhizomucor miehei lipase and Candida antarctica lipase B with a mass ratio of 0.2-0.8:

1.

2. The production method according to claim 1, characterized by, In step (1), the basic nutrient solution comprises the following components in percentage by weight: 1-5% glucose, 0.1-10% peptone, 0.1-1% yeast powder, 0.001-0.01% sodium dihydrogen phosphate, 0.001-0.01% potassium dihydrogen phosphate, and the rest is water.

3. The preparation method according to claim 1, characterized in that, In step (1), after the bacteria are activated, 5-10% v / v of the bacteria are inoculated into the basic nutrient solution.

4. The method of claim 1, wherein, In step (1), the fermentation temperature is 26-30℃, and the fermentation time is 24-72h.

5. The preparation method according to claim 1, characterized in that, In step (2), the amount of the lipase is 1-10% of the mass of the glycerol.

6. The method of claim 1, wherein, In step (2), the mass ratio of the fermentation product to the glycerol is 1:1-1.

5.

7. The preparation method according to claim 1, characterized in that, In step (2), the esterification reaction temperature is 40-60℃, and the reaction time is 5-10h.

8. The method of claim 1, wherein, In step (1), the oil is selected from plant oil; The plant oil is selected from one or more of the following: flaxseed oil, soybean oil, rapeseed oil, peanut oil, corn oil, sunflower seed oil, oil tea seed oil, coconut oil, palm oil, palm kernel oil, olive oil, oil olive pomace oil, walnut oil, rice bran oil, rice oil, cottonseed oil, perilla seed oil, safflower seed oil, grape seed oil, tea seed oil, peony seed oil, sesame oil, wheat germ oil, king tree seed oil, shan-guan fruit oil, sea-buckthorn seed oil, DHA algal oil, medium-chain triglyceride, medium-long chain fatty acid edible oil, hempseed oil, prickly ash seed oil, prickly ash oil, pumpkin seed oil, chili oil, almond oil, and macadamia nut oil; Or, the oil is selected from animal oil.

9. The method of claim 1, wherein, In step (1), the oil is selected from one or both of perilla seed oil and safflower seed oil.

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