Novel antineoplastic compound Ananaside B in thelenota ananas

A technology of anti-tumor drugs and compounds, applied in the field of medicine

Inactive Publication Date: 2008-08-20
巫军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The chemical structure and antitumor activity of the saponins contained in it have not been reported so far.

Method used

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  • Novel antineoplastic compound Ananaside B in thelenota ananas
  • Novel antineoplastic compound Ananaside B in thelenota ananas
  • Novel antineoplastic compound Ananaside B in thelenota ananas

Examples

Experimental program
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Effect test

Embodiment Construction

[0029] The following examples further describe the separation and preparation of Ananaside B.

[0030] Wash 1000 grams of Plum Blossom Ginseng, chop it, soak it in turn with 5 times the amount (weight) of 95%, 85%, and 85% ethanol respectively, soak for 7 days each time, combine the ethanol extract, reclaim the ethanol under reduced pressure, and obtain the flow leaching paste. Dissolve the liquid extract in 3 times the amount of water, filter, and pass the filtrate through a DA201-B macroporous resin column, elute with water and 50% ethanol in sequence, collect the 50% ethanol eluate, recover ethanol under reduced pressure until dryness, and obtain total saponins 5.6 grams. The total saponins were subjected to silica gel column chromatography, eluted with a solvent of dichloromethane:methanol:water at a ratio of 7:3:1, and the fractions containing AnanasideB were collected, and then subjected to C 18 Reversed-phase column chromatography, gradient elution with methanol: wate...

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Abstract

The invention relates to the field of medical technology, in particular to an anti-cancer compound Ananaside B which is separated from thelenota ananas, and the molecular formula is C54H87O26SNa. The chemical structure and the spatial configuration of the compound are determined by various modern spectral analysises, in particular to the comprehensive analysis by applying the advanced two-dimensional nuclear magnetic resonance spectra and high resolution mass spectra. In vitro anti-tumor trials show that the compound has significant inhibition function on A-549 lung cancer and IA-9 ovarian cancer and other 10 kinds of tumor cell strains. The compound can provide a lead compound for the development of new anti-tumor drugs, which has important values for the development and the utilization of the marine medicinal and biological resources of China.

Description

technical field [0001] The invention relates to the technical field of medicine, and is a new antitumor compound Ananaside B isolated from marine animal ginseng. Background technique [0002] Plum ginseng (Thelenota ananas J.), also known as "pineapple ginseng", belongs to the sea cucumber family Aposticaceae of the marine echinodermata. Its body is very large, generally 700mm in length, 100mm in width, and 80mm in height. It is widely distributed in my country's Xisha Islands and the southern tip of Taiwan. Its body is rich in protein, polysaccharides, vitamins, and saponins, which have the effects of nourishing the liver and kidney, benefiting essence, and nourishing blood. The chemical structure and antitumor activity of the saponins contained in it have not been reported so far. Contents of the invention [0003] The present invention extracts and separates a new saponin compound from plum blossom ginseng grown in the sea area of ​​Fujian, China, named Ananaside B, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00C07J75/00A61K31/58A61P35/00A61K35/56A61K35/616
Inventor 巫军
Owner 巫军
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