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A method for identifying plum blossom ginseng using specific peptide groups

A plum flower ginseng, specific technology, applied in the biological field, can solve problems such as lack of technical level, deceiving consumers, etc.

Active Publication Date: 2018-06-22
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the lack of a particularly effective method of identifying sea cucumber species at present, the method of identifying sea cucumber species is only based on traditional appearance observation, lacking technical support, resulting in many unscrupulous merchants cheating consumers by shoddy goods and false ones. Therefore, it is necessary to An efficient and accurate method for identifying sea cucumbers

Method used

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  • A method for identifying plum blossom ginseng using specific peptide groups
  • A method for identifying plum blossom ginseng using specific peptide groups
  • A method for identifying plum blossom ginseng using specific peptide groups

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1, Sequence Source and Alignment Information of Specific Polypeptides

[0037] 1. The unique peptide ADIAEDSLK source of plum blossom ginseng

[0038] The unique peptide ADIAEDSLK of plum blossom ginseng comes from the protein tropomyosin [Apostichopus japonicus], its Accesion number on NCBI is gi|302340969, the sequence is shown in the following SEQ ID NO.5, the analysis found that the sixth Glu of ADIAEESLK was mutated into Asp, The obtained peptide ADIAEDSLK was compared with NCBI blast, and was not found to be consistent with any other species:

[0039] SEQ ID NO.5:

[0040] MDTIKKKLSQLKADKEKALDEKDVAEASMKEAMERVEQVNDENKELQTRIKQLETELDDTSEKLNTTVIKCEAAEKAQQTAEEEMANLQRKLQLTEEELSRSEERVADLQSKYTDIEQSSEENERQRKVLESRSAADDERMSELETQVMSSKTSLEDSDRKYDEASRKLTVTEEELARSEERSAAFESSLSQMKEELHQLHNNVKSLEAQEEKFTENEEMYEKKVRDLEDKLKVAEDRADIAEESLKSLKTSLDQLEDELMIEKEKVREMTEEMERTIQELNFEV

[0041] Using AB SCIEX 5600 mass spectrometer (AB 5600) detects the fragmentation diagram and mass ...

Embodiment 2

[0066] Embodiment 2, sea cucumber sample processing and detection steps

[0067] The steps of analyzing the sea cucumber samples to be tested include

[0068] (1) Sample pretreatment steps:

[0069] (1) Weigh 1g sea cucumber sample and homogenize it into a powder state, add 10mL protein extract (8M urea, 50mM NH 4 HCO 3 ) shake to extract protein, centrifuge at high speed and low temperature (20000r / min), take 200ul of supernatant and transfer to 1ml EP tube;

[0070] (2) Take 2μL of 1mol / L DTT (0.154g of dithiothreitol DTT dissolved in 1mL of deionized water) and add it to 100μL of the above protein solution and react at 37°C for 1 hour;

[0071] (3) Take 10 μL of freshly prepared IAA (accurately weigh 0.185g of iodoacetamide IAA and dissolve it in 1mL of deionized water to prepare a 1mol / L IAA mother solution for later use. The preparation process needs to be protected from light, and it is ready to use) and added to In the above reaction solution that has been cooled to...

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Abstract

The present invention relates to a method for identifying Prunus ginseng (Thelenota ananas) using a specific peptide group, and a set of control polypeptides for identifying Plum ginseng by mass spectrometry. The sequence of the polypeptide is: SEQ ID NO.1: ADIAEDSLK ; SEQ ID NO. 2: AGPAGDDAPR; SEQ ID NO. 3: MPIPLLEGK; SEQ ID NO. 4: LDIPLLEGK. It has been identified that the peptide described in SEQ ID NO.1: ADIAEDSLK is a proprietary peptide of Prunella ginseng, which has not been expressed in other species; SEQ ID NO.2: AGPAGDDAPR; SEQ ID NO.3: MPIPLLEGK; SEQ ID NO. 4: Although the peptides described in LDIPLLEGK exist in other species, they are not expressed in sea cucumbers, Haitian melons, American ginseng, Mexican ginseng, and American ginseng. The above peptides are exclusive peptides of plum blossom ginseng.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying plum blossom ginseng (Thelenota ananas) by using a specific peptide group. Background technique [0002] Sea cucumbers are rich in acidic mucopolysaccharides, which have the effects of immunomodulators, anticoagulants, blood sugar control and blood fat reduction on the human body. Therefore, sea cucumber enjoys the reputation of "treasure house of nutrition", and modern people regard sea cucumber as "the first of the eight delicacies of seafood". There are many kinds of sea cucumbers in the market, and the prices of different sea cucumbers vary greatly due to their nutritional value and taste. Plum ginseng (Thelenota ananas) has the same economic value as gray sea cucumber. It is not only a tonic, but also can treat diseases and fight cancer. It has anti-aging effects, contains high protein, is rich in minerals, and does not contain cholesterol, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62
Inventor 李八方张鸿伟赵雪
Owner OCEAN UNIV OF CHINA
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