618 results about "Mass spectrography" patented technology

Methods, systems and kits for the simultaneous or sequential analysis of one or more hormones by mass spectrometry are disclosed. The methods require minimal sample size and minimal preparation time. The methods include ionizing the hormones and analyzing the hormones by mass spectrometry. In addition, methods, systems and kits for the simultaneous or sequential analysis of thyroid hormones are disclosed including ionization of the thyroid hormones in the negative mode using an electrospray source.

A new ionization source named Surface Activated Chemical Ionization (SACI) has been discovered and used to improve the sensitivity of the mass spectrometer. According to this invention the ionization chamber of a mass spectrometer is heated and contains a physical new surface to improve the ionization process. The analyte neutral molecules that are present in gas phase are ionized on this surface. The surface can be made of various materials and may also chemically modified so to bind different molecules. This new ionization source is able to generate ions with high molecular weight and low charge, an essential new key feature of the invention so to improve sensitivity and reduce noise. The new device can be especially used for the analysis of proteins, peptides and other macromolecules. The new invention overcomes some of the well known and critical limitations of the Electrospray (ESI) and Matrix Assisted Laser Desorption Ionization (MALDI) mass spectrometric techniques.

An open probe method for sample introduction into a mass spectrometer is disclosed, comprising the steps of: loading a sample holder with sample compounds to be analyzed; heating a probe oven; introducing said sample compounds in said sample holder into said heated probe oven; flowing inert gas into said heated probe oven; vaporizing said sample in said heated probe oven by the combined effect of oven temperature and inert gas flow; entraining said vaporized sample in said inert gas; and, transferring said vaporized sample in inert gas into an ion source of a mass spectrometer; wherein said heated probe oven remains open to the ambient atmosphere during sample introduction and analysis; said inert gas is flowing in said heated probe oven in two directions of a transfer line to a mass spectrometer ion source and to the oven opening; said vaporized sample in inert gas is transferred through a heated transfer line directly into the ionization chamber of an ion source of a mass spectrometer. An apparatus for this method of sample introduction is also disclosed. The primary advantage of this method and apparatus is that the heated probe oven remains open to the ambient atmosphere during sample introduction and analysis thereby enabling faster sample analysis.

Methods and apparatuses for analyzing proteins and other biological materials and xenobiotics within a sample. A specimen is generated, which may include an energy absorbent matrix. The specimen is struck with laser beams such that the specimen releases proteins. The atomic mass of the released proteins over a range of atomic masses is measured. An atomic mass window of interest within the range of atomic masses is analyzed to determine the spatial arrangement of specific proteins within the sample, and those specific proteins are identified as a function of the spatial arrangement. By analyzing the proteins, one may monitor and classify disease within a sample.

The invention provides a method for detecting volatile organic compound in a cigarette filter, which comprises the following steps: a) preparing a sample; b) preparing a matrix correcting agent; c) preparing an interior label solution; d) preparing a standard sample; e) preparing a standard working solution; f) performing detection by using headspace-gas chromatography/ mass-spectrography; and g) calculating a detection result of the volatile organic compound in the sample. The invention establishes the method for detecting the volatile organic compound residue in the cigarette filter for the first time. By using the saturation sodium chloride aqueous solution as the matrix correcting agent and the fluorobenzene as the interior label, the matrix effect of a solid absorption matrix (namely the cigarette filter) is efficiently reduced and the error caused by the poor repeatability of the pre-treatment and the low precision of instrument is reduced. In the whole process, no organic solvent is used. The method has the advantages of environmental friendliness, energy-saving and low consumption. The detecting method has few interference factors and good repeatability, is accurate in measuring result, is simple to operate, and can be used for obviously increasing the sensitivity of headspace analysis.

The method for analyzing diesel hydrocarbon composition by using solid phase extraction and mass spectrography includes the following steps: using solid phase extraction process to separate saturated hydrocarbon and aromatic hydrocarbon in diesel sample, collecting all the saturated hydrocarbon solution and aromatic hydrocarbon solution, respectively adding equivalent internal standard material, then respectively sampling and making gas chromatography and mass spectrography; utilizing gas chromatomap of aromatic hydrocarbon and saturated hydrocarbon to calculate relative content of both them, and utilizing mass spectrogram to obtain hydrocarbon composition of aromatic hydrocarbon and saturated hydrocarbon; according to the relative content of saturated hydrocarbon and aromatic hydrocarbon using normalization-calculation method to obtain the hydrocarbon composition of diesel.

The invention relates to electronic cigarette liquid and a quality detecting method. The raw materials of the electronic cigarette liquid are cigarette industry finished cigarette tobacco supercritical fluid extracting substances, other components are added, even mixing is carried out according to weight percentage, and then the liquid is prepared. According to the formula, the liquid comprises 10%-30% of finished cigarette tobacco extracting substances, 5%-15% of tobacco flavor, 2%-10% of deionized water and 10%-30% of glycerine, and the rest is propylene glycol. A gas chromatography-mass spectrography method is used for analyzing the extracting substances, a chromatographic peak area normalization method is used for determining the relative content of various volatile components, and according to the key chemical components which determine characteristic aroma style and the magnitude of the relative content, the quality of the electronic cigarette liquid is evaluated. According to the electronic cigarette liquid, the cigarette finished cigarette tobacco supercritical fluid extracting substances are used as main components, the aroma and the taste of an electronic cigarette are made similar to the taste and sense judgment of a traditional cigarette to the maximum degree, and the problem that single cigarette regulated cigarette liquid taste is single and is not harmonious is solved. Meanwhile, cigarette liquid flavor component evaluation is objective and accurate through the gas chromatography-mass spectrography method.

The invention relates to the field of analytical chemistry and food safety, in particular to a detection method for residual quantity of multiple preservatives in cosmetics. According to a gas chromatography-mass-spectrography detection method which can detect the residual quantity of multiple preservatives in cosmetics simultaneously, sample extraction and purification are respectively processed according to differences of sample shapes and properties, and then a gas chromatography-mass spectrometer is adopted to carry out qualitative determination and quantitative assay. The detection method achieves simultaneous detection of 21 preservatives, and has the advantages of being simple and convenient, fast, sensitive, and the like. The detection minimum, the recovery rate and the degree of precision of the method all meet relevant demands for preservative detection in the cosmetics at home and abroad, so the method is suitable for detection and monitoring of the preservatives in the cosmetics.

A gas chromatography/mass spectrometry system includes two sample introduction parts, a gas chromatography part having two columns, a column oven for housing the two columns in a parallel configuration, an interface part through which an outlet end of each of the two columns is inserted, and a mass spectrometry part. The mass spectrometry part has an ionization chamber to which the outlet ends of the columns inserted through the interface part are connected, a mass separation part, an ion detector, and a vacuum chamber for housing the ionization chamber, the mass separation part, and the detector. Because the outlet ends of the parallel columns are both connected to the ionization chamber, the carrier gas flow and line velocity are the same as though there is one column, thereby avoiding line stagnation. Since analyses can be performed selectively with a single system, results having high reliability and precision can be obtained.

The invention discloses a hydrophilic interaction chromatography-tandem mass spectrometry detection method of phospholipids in Metapenaeus ensis. The method includes the steps of firstly, performing corresponding preprocessing on ground Metapenaeus ensis to obtain crude lipid extract; secondly, performing liquid phase separation on the crude lipid extract, wherein a chromatographic column is a YMC Triart diol HILIC column, a gradient elution method is used, a gradient system comprises a flowing phase A and a flowing phase B, the flowing phase A is an acetonitrile solution containing 53mmol/L formic acid, and the flowing phase B is an aqueous solution containing 60mM ammonium formate and 53mM formic acid; thirdly, performing mass spectrometric detection analysis on the elution liquid obtained in the second step. By the method, the total content of PE, PS and PC in the Metapenaeus ensis can be detected accurately, and mass spectrometry identification of various molecular species in the PC, PE and PS phospholipids can be achieved.

An apparatus and method for performing mass spectroscopy uses an ion interface to provide the function of removing undesirable particulates from an ion stream from an atmospheric pressure ion source, such as an electrospray source or a MALDI source, before the ion stream enters a vacuum chamber containing the mass spectrometer. The ion interface includes an entrance cell with a bore that may be heated for desolvating charged droplets when the ion source is an electrospray source, and a particle discrimination cell with a bore disposed downstream of the bore of the entrance cell and before an aperture leading to the vacuum chamber. The particle discrimination cell creates gas dynamic and electric field conditions that enables separation of undesirable charged particulates from the ion stream.

The invention discloses a segregation detection method of drapery tartan calcium and optical isomer (impurity), which is characterized by the following: adapting high effective gas-chromatography or high effective gas-chromatography mass spectrography to detect the optical isomer of drapery tartan calcium; segregating the drapery tartan calcium and optical isomer of drapery tartan calcium.

The invention provides methods and apparatus for automated sample preparation for MALDI-TOF MS using columns in combination with a liquid handling system. The samples are typically biological such as serum or urine. The columns typically include a bed of media positioned within a modified pipette tip. In some embodiments, the invention provides methods for storing the prepared samples.

The invention discloses a method for measuring the phthalate ester content of tipping paper for tobaccos. The method comprises the steps of: preparation of internal standard extract liquor, standard working solution and sample solution, gas chromatography-mass spectrography, calculation of measurement results and the like. The improved and optimized measurement method has the advantages of operation simplicity and accuracy, high sensitivity, high recovery rate, high repeatability and the like. Errors caused by pretreatment or apparatus precision and the like are effectively reduced by utilizing an internal standard quantity. Under adopted chromatographic conditions, seven target compounds and internal standard chromatographic peaks have relatively higher degrees of separation, relatively higher linear correlation and the detection limit of 0.01 to 0.03 mu g per centimeter spare. By the method, a standard recovery rate is 94 to 106 percent and an average relative standard deviation is 3.4 to 4.8.