337 results about "Mass spectrography" patented technology

Provided herein are methods for analyzing a biological sample that include contacting the biological sample with a non-conductive substrate; and contacting a matrix to a surface of the biological sample. Further methods optionally include performing mass spectrometry analysis; determining presence of a first analyte in the biological sample; and / or analyzing a second analyte on the surface of the biological sample to determine presence of the first and the second analyte in the biological sample.

The invention belongs to the field of functional proteomics research, and particularly relates to a combined analysis method for analyzing interaction between a small molecule substance and a targeted protein, which is used for analyzing the small molecule substance and the protein by adopting LiP-MS (Limited Protein-Mass Spectrometry) technology and phosphorylated proteome combined analysis. According to the method, the optimized LiP-MS technology is adopted, the target protein can be directly recognized under the complex proteome background without enriching specific protein, and the problem that low-abundance protein is difficult to detect is solved; phosphorylated proteome abundance transformation protein is analyzed by combining high-resolution mass spectrometry, so that the recognition capability on a drug target and a shear variant thereof is enhanced, and the detection sensitivity is improved.

The invention provides a rapid quantitative analysis method for an outer vesicle proteome, and belongs to the technical field of quantitative analysis of the outer vesicle proteome. According to the rapid quantitative analysis method for the outer vesicle proteome, firstly, a high-quality outer vesicle sample is obtained through low-speed centrifugation, ultra-speed centrifugation and membrane filtration; and obtaining proteome data by utilizing two-dimensional electrophoresis and mass spectrometry. Then, a mathematical model equation set is constructed for preliminary analysis, a protein interaction network is optimized through an embedded game optimization index model, and feature extraction and quantitative analysis are conducted through a convolution parameter adaptive function and a residual network deep learning model; finally, a quantitative analysis result containing the protein expression level and the function importance is generated in combination with the multi-dimensional protein expression contribution index, and the technical problem that in the prior art, in the rapid quantitative analysis process of the outer vesicle proteome, the protein component complexity is high, and consequently the quantitative precision is low is solved.

The invention discloses a method for segmenting different strata series of stratified shale and identifying oiliness, and belongs to the field of oil-gas exploration. The invention discloses a strata division method based on XRF (X-Ray Fluorescence) mineral scanning and single polarization-fluorescence-laser confocal optical lens combined observation, which realizes accurate identification and segmentation of micron-sized strata boundaries of a layered rock core sample from different scales, and performs group component extraction on segmented independent strata. And carrying out chromatography-mass spectrometry analysis and FT-ICR MS analysis on the extracted saturated, aromatic, non-aromatic, leached and other family components. The TSF spectrum, light and heavy components, biomarker compounds, non-hydrocarbon compounds and other data obtained through the experiments are comprehensively analyzed, layering scale oiliness difference quantification and oil source comparison are achieved, and the shale oil micro-migration phenomenon is determined. The influence of the heterogeneity of the layered rock core sample on a result obtained by a traditional analysis method is overcome. According to the technology, direct evidences are provided for accurate prediction of shale oil desserts, exploration risks are reduced, and economic benefits are improved.

The invention relates to a screening method and an activity evaluation method of a natural antioxidant, and the screening method is characterized in that a cerium dioxide-based nano composite material with a specific structure is used as oxidase, a nano oxidase + natural antioxidant reaction system is constructed, and the activity of the natural antioxidant is evaluated through a high performance liquid chromatography-ultraviolet detector-tandem mass spectrometry analysis technology. Screening of the natural antioxidant is realized; according to the activity evaluation method, a cerium dioxide-based nano composite material with a specific structure is used as oxidase, a nano oxidase + natural antioxidant + TMB reaction system is constructed, and the activity evaluation of the natural antioxidant is realized through an oxidized TMB photo-thermal detection technology. According to the method disclosed by the invention, the used reaction system is high in applicability, high in accuracy and good in selectivity.

The invention relates to the technical field of nutritious food configuration, and discloses a system and a method for configuring personalized nutritious food, and the method for configuring personalized nutritious food comprises the following steps: collecting spectral characteristic data and chemical component data of a food material sample by adopting a hyperspectral imaging and mass spectrum combined technology, and obtaining holographic chemical fingerprint information; processing the collected spectral data by using a multi-scale feature extraction algorithm, extracting multi-level feature information of the food materials, and carrying out standardization and dimension reduction processing on the feature data; according to the method, high-precision prediction of the nutrient content of the food material is realized through combination of hyperspectral imaging and mass spectrometry technologies and a deep learning model, so that the nutrient content prediction accuracy and the configuration scheme accuracy are improved, the nutrient balance achievement rate is also improved, and meanwhile, the limitation of a static database is broken through; real-time nutritional ingredient analysis of any food material is achieved, the method can adapt to individual differences and freshness changes of the food materials, and accurate configuration based on actual food material characteristics is provided.

The invention relates to the technical field of molecular biological detection, in particular to a primer, a probe group and a kit for multiplex nucleic acid mass spectrometry detection of porcine diarrhea pathogens and application. By adopting a matrix-assisted laser desorption / ionization time-of-flight nucleic acid mass spectrometry technology, eight main pathogens such as PEDV, TGEV, PDCoV, PoRV, SADS-CoV, PBoV, HEV and salmonella can be rapidly, sensitively and specifically detected at the same time. By designing the specific primer and the unexpanded probe and combining multiple PCR amplification and mass spectrometry, the method realizes high-throughput and multi-pathogen simultaneous detection. Experimental results show that the method has high sensitivity and high specificity, has no false positive reaction, and shows 100% repeatability in 60 repeated experiments. Compared with a qPCR method, the detection consistency is as high as 96.2%. The method has the advantages of being easy and convenient to operate, high in detection speed and high in adaptability, can be widely applied to efficient monitoring of porcine diarrhea pathogens, and provides important technical support for early diagnosis, prevention and control of porcine diseases.

The invention discloses a white spirit flavor recognition method based on machine learning, and belongs to the technical field of food detection and artificial intelligence. Aiming at the problems of high subjectivity of manual evaluation, low efficiency of mass spectrometry, noise sensitivity of a machine learning model and the like in the prior art, the method provides a solution integrating solvent background deduction and feature weight screening. The method specifically comprises the following steps: diluting a white spirit sample with methanol according to a volume ratio of 1: 10, collecting mass spectrum data through GC-MS, and dynamically deducting a methanol background peak; a BP neural network (GABP) optimized by a genetic algorithm is utilized to automatically analyze a weight coefficient of each molecular peak to flavor classification, and key features are screened; a classification model is constructed based on XGBoost, and parameters are optimized through cross validation, so that automatic judgment of the flavor type, authenticity and quality of the white spirit is realized. According to the method, through data dimension reduction and model collaborative optimization, the overfitting problem caused by high noise and high redundancy of mass spectrum data is solved, the classification accuracy is remarkably improved, and the method can be extensively applied to white spirit brand identification, process optimization and market quality supervision.

The present invention relates to a pretreatment method for serum liquid chromatography-tandem mass spectrometry analysis, comprising the following steps: (S1) mixing a serum sample with an acetonitrile solution containing formic acid, ultrasonicating, vortexing, and centrifuging, taking the supernatant and diluting it with an aqueous solution containing formic acid to obtain a liquid to be purified; (S2) mixing the liquid to be purified with PCN-777, ultrasonicating, vortexing, and centrifuging, taking the supernatant and obtaining a liquid to be tested; the PCN-777 is C 24 H 23 N3O 16 Zr3 phospholipid removal material, the PCN-777 is C 24 H 23 N3O 16 Zr3. The present invention uses a known MOFs material PCN-777 to develop a pretreatment method for serum ultra-high performance liquid chromatography-tandem mass spectrometry analysis, which can specifically, quickly and effectively remove different types of phospholipids in serum, and achieve efficient recovery and detection of pesticides and veterinary drugs, avoiding interference from phospholipids.

The invention relates to the field of biomedicine, and discloses a method for screening KRAS mutant interacting proteins, an HEK293T cell line for continuously expressing an HA-Turbo ID-KRAS variant is established by utilizing lentivirus transduction, the stability of transgene expression is ensured through puromycin screening, and variation caused by transient transfection is reduced as much as possible. After a Turbo ID mediated proximity marker is activated by biotin treatment, KRAS proximity binding proteins are covalently biotinylated, captured by NeutrAvidin bead purification, and analyzed by quantitative mass spectrometry. According to the invention, two potential KRAS mutation treatment target proteins of LZTR1 and LAMTOR1 can simultaneously inhibit activation and efficacy exertion of three different mutants of G12C, G12D and G12V of the G12 mutant, compared with drugs on the market that a single target of G12C directly targets the KRAS mutant, a brand new KRAS mutation targeting intervention strategy is provided, the clinical curative effect can be further improved, and the occurrence rate of secondary drug resistance can be effectively reduced.

The invention discloses a polygonatum sibiricum medicinal material species identification method based on a polygonatum sibiricum characteristic compound and application of the polygonatum sibiricum medicinal material species identification method. The invention discloses a polygonatum sibiricum medicinal material species identification method based on a polygonatum sibiricum characteristic compound. The method comprises the following steps: 1) preparing a polygonatum sibiricum rhizome sample solution; 2) analyzing different sealwort rhizome metabolites by liquid chromatography-mass spectrometry; and 3) discriminating the species of the rhizoma polygonati medicinal material, namely discriminating the species of the rhizoma polygonati medicinal material if the rhizoma polygonati rhizome metabolite contains a corresponding characteristic compound. The polygonatum sibiricum variety is polygonatum kingianum, polygonatum sibiricum, polygonatum cyrtonema and polygonatum odoratum. According to the application of the polygonatum kingianum characteristic compounds in polygonatum kingianum medicinal material species identification, each polygonatum kingianum species selects three representative characteristic compounds which are relatively high in content, have functional specificity representatives and are common in the market, the species of the polygonatum kingianum medicinal material can be identified as long as the three representative characteristic compounds are contained at the same time, the accuracy is high, the number of components to be detected is small, and the accuracy is high. The detection period is short.

The invention belongs to the technical field of textile organic matter detection, and relates to a PTR-MS-based rapid mass spectrometry system and method for volatile organic compounds in textiles. Through the synergistic effect of the temperature-adjustable constant-temperature heater, the optimally designed gas exchange chamber and the mass flow controller, the limitation of complex background interference and a single temperature mode is effectively solved; the temperature-adjustable constant-temperature heater can gradually adjust the temperature according to the boiling point characteristics of VOCs, the release efficiency of substances with different boiling points is optimized, interference caused by too fast release of substances with low boiling points is avoided, and therefore the detection accuracy is improved; the optimally designed gas exchange chamber is used for clamping a textile sample through two semi-spindle-shaped cavities to form a closed space, so that the contact area and time of the sample and carrier gas are effectively controlled, the interference of environmental pollutants is reduced, and the detection sensitivity is improved; and meanwhile, the mass flow controller accurately controls the flow velocity of the carrier gas, so that VOCs are fully carried into a mass spectrum acquisition instrument, and detection errors caused by improper flow velocity of the carrier gas are avoided.

Methods and systems for multi-beam, parallel-beam, deterministic, or super mass spectrometry that include an ion source that produces ions, and two or more ion trapping devices or mass spectrometers, each having an independent sampling inlet. The two or more ion trapping devices or mass spectrometers receive the ions from the ion source via the sampling inlet of each of the ion trapping devices or mass spectrometers such that each sampling inlet provides an ion beam to each corresponding ion trapping device or mass spectrometer.

This invention discloses an AfBPP-based active molecular probe for thiostreptin, its preparation, and its application, belonging to the field of chemical biology. This invention introduces a bifunctional tag integrating a photocrosslinking group (bisacrididine) and a bioorthogonal reactive group (alkynyl group) into the structure of thiostreptin. While retaining the original biological activity of the parent compound, it endows the probe with highly efficient probe function, solving the technical problem of target loss during washing and purification of traditional non-covalent probes. After target labeling, the probe can specifically connect to reporter groups such as fluorescein or biotin through click-chemical reactions, achieving efficient enrichment of drug targets. Combined with mass spectrometry analysis, it enables global identification of potential targets in cells or complex biological samples at the omics level, such as chemical proteomics, providing a powerful molecular tool for in-depth revelation of the potential targets and pharmacological mechanisms of thiostreptin.

The present invention discloses a methane calcium chain reforming single fluidized bed reaction system and reaction method, comprising a cylindrical quartz tube filled with reaction particles, an adjustable light source located beside the cylindrical quartz tube with its radiation focus located at the top center of the reaction particles, a thermal insulation layer wrapped around the outside of the cylindrical quartz tube, a position adjustment device for driving the cylindrical quartz tube to move and rotate, thermocouples uniformly distributed within the cylindrical quartz tube, a gas distribution system for supplying reaction gas into the cylindrical quartz tube, a mass spectrometer connected to the gas outlet of the cylindrical quartz tube via a drying tube, and a control system connected to the thermocouple, the gas distribution system, and the mass spectrometer, respectively. The thermal insulation layer is provided with an inlet on the side facing the adjustable light source. The present invention aims to achieve efficient energy absorption and conversion while reducing heat dissipation, capturing CO2 while obtaining high-value-added hydrocarbon fuels.

The invention discloses a gas exchange device in front of a sample introduction cone of an inductively coupled plasma mass spectrometer, the gas exchange device in front of the sample introduction cone is arranged outside an ICP torch tube in a sleeving manner, and the gas exchange device in front of the sample introduction cone is mounted on a partition plate around the sample introduction cone; the sample injection cone front gas exchange device comprises a porous breathable layer and a shell; an argon inlet is formed in one end, close to the sample introduction cone, of the shell; one end, far away from the sample introduction cone, of a space between the porous breathable layer and the ICP torch tube is opened and is communicated with the atmosphere. According to the invention, the environmental gas components in the space between the ICP torch tube and the mass spectrum interface device are exchanged into the preset clean gas, so that the sample ionization process is not interfered by the environmental air components, a lower noise baseline is obtained, and meanwhile, the generation of environmental component ions such as nitrogen oxide ions which possibly interfere mass spectrum analysis is reduced; the sensitivity of mass spectrometry detection and the environment anti-interference capability are improved.

The invention relates to the technical field of floridoside and an isomer monomer thereof, and discloses a method for preparing high-purity floridoside and an isomer monomer thereof on a large scale, which is used for carrying out large-scale preparation and purification research on the high-purity floridoside and the isomer monomer thereof and preparing the floridoside and the isomer monomer thereof from a porphyra haitanensis crude extract. And the structure and purity of the compound are identified. The method comprises the following steps: firstly, by utilizing a DART-MS technology, through a single factor experiment, optimizing experiment parameters such as extraction times and elution, and obtaining optimal extraction efficiency; and secondly, based on a two-step purification method of a prep-HPLC (Prep-High Performance Liquid Chromatography) technology, enriching the target compound by using a reversed-phase (C18) mode stationary phase in the first step, and separating the pair of isomers by using a normal-phase (NH3) mode stationary phase in the second step. The obtained floridoside and isomer monomers thereof are subjected to structure and purity identification by mass spectrometry (MS) and nuclear magnetism (NMR). Results are as follows: the two compounds are identified as floridoside and isomers thereof through high-resolution mass spectrometry, and the purities of the floridoside and the isomers thereof are up to 99.5% or above.

A data-dependent analysis (DDA) mass spectrometry technique involves ionizing a sample to generate sample ions, analyzing the sample ions by performing one or more MS1 mass analysis scans to obtain MS1 ​​data, identifying one or more precursor ions from the MS1 data, and subsequently analyzing the sample ions by performing one or more MS2 mass analysis scans. Each MS2 scan targets one of the one or more precursor ions identified from the MS1 data.The procedure further includes, for each of the one or more precursor ions identified from the MS1 data: determining a value from the MS1 data that is indicative of a collision cross section (CCS) of that precursor ion; and selecting, based on the CCS-indicative value(s), which precursor ion(s) the one or more MS2 bulk analysis scans should target, and / or determining, based on the CCS-indicative value(s), an order in which the one or more MS2 bulk analysis scans should target the precursor ions.

Methods of labeling, processing and analyzing protein samples. Surface-accessible residues in a protein sample (such as a proteome) can be labeled using a carbodiimide coupling agent an amine comprising a click chemistry handle, such as glycine propargyl amide. The click chemistry handle can then be derivatized using high-yield "click" chemistry to form a stable linkage with an enrichable label. The proteome can then be digested, peptides that have been modified can be enriched, and the enriched peptides can be analyzed, for example, by mass spectrometry. The analyzed peptides can constitute a highly enriched subset of the original peptides while still representing many sites of modification on many proteins (~95%) originally present in the proteome. The methods can be used to identify solvent-accessible regions of proteins, conformational changes, and drug targets across a whole proteome.

Methods for mass spectrometry, which include the following: Predictions of one or more first reaction products that may result from subjecting an analyte to one or more reactions of interest, Calculating one or more first masses or mass / charge ratios and one or more first ion mobility values, collision cross sections or interaction cross sections of at least some first reaction products that can be generated under first conditions using the one or more first reaction products, Calculating one or more second masses or mass / charge ratios and one or more second ion mobility values, collision cross sections or interaction cross sections of at least some second reaction products that can be generated under two different conditions using one or more first reaction products, Generating third ions from a sample and experimentally determining one or more third masses or mass / charge ratios and one or more third ion mobility values, collision cross sections or interaction cross sections of at least some of the third ions under the first conditions, Generating fourth ions from the sample and experimentally determining one or more fourth masses or mass / charge ratios and one or more fourth ion mobility values, collision cross sections or interaction cross sections of at least some of the fourth ions under the second conditions, and Comparing the first and second masses or mass / charge ratios with the third and fourth masses or mass / charge ratios and / or comparing the first and second ion mobility values, collision cross sections or interaction cross sections with the third and fourth ion mobility values, collision cross sections or interaction cross sections to confirm the presence and / or absence of one or more first reaction products in the sample.

The invention discloses a dual time-of-flight mass spectrometer and a detection method. The mass spectrometer comprises an ion generation transmission area, a first time-of-flight mass spectrometry analysis area, a secondary ion transmission area and a second time-of-flight mass spectrometry analysis area, the ion generation and transmission area is used for generating ions to be detected and transmitting the ions to be detected to the first time-of-flight mass spectrometry area; the first time-of-flight mass spectrometry analysis area is used for carrying out time-of-flight mass spectrometry analysis on the to-be-detected ions expelled by the ion generation and transmission area; the secondary ion transmission area is used for transmitting the to-be-detected ions penetrating through the reflecting area of the first time-of-flight mass spectrometry area to the second time-of-flight mass spectrometry area; and the second time-of-flight mass spectrometry analysis area is used for carrying out time-of-flight mass spectrometry refinement analysis on the to-be-detected ions penetrating through the reflecting area of the first time-of-flight mass spectrometry analysis area. According to the invention, a wide mass-to-charge ratio measurement range and fine measurement of a signal of a certain section of mass-to-charge ratio can be simultaneously realized through double mass spectrometry.

The invention belongs to the field of biological medicine, and particularly relates to a protein marker for lung metastasis of primary liver cancer and application of the protein marker. The invention discloses a protein marker for lung metastasis of primary liver cancer. The protein marker is selected from at least one of rs2 protein, rcsd1 protein, rbbp9 protein, prl6a1 protein, ubxn4 protein and mospd2 protein. According to the invention, mass spectrometry is carried out by using an HPLC-MS / MS mass spectrometry system and an unlabeled quantitative proteomics method; and then searching mass spectrum data by utilizing a non-limited amino acid protein modification analysis method, and verifying whether differential protein substances have significance or not according to P-value, so as to finally obtain six proteins with significant difference, namely, the rs2 protein, the rcsd1 protein, the rbbp9 protein, the prl6a1 protein, the ubxn4 protein and the mospd2 protein. The protein marker provided by the invention has higher sensitivity and specificity, and the combination of the six proteins has better detection efficacy.

The disclosure relates generally to compounds and methods for selective gas phase enrichment of modified peptides for reactive protein profiling.

A gas analysis apparatus and method are disclosed that can perform measurements in direct and collection modes without complex control. The gas analysis apparatus 100 according to this disclosure includes: a branching component 20 for branching a target gas; a mass spectrometer 80 for mass spectrometry analysis of one branch of the target gas; a collection component 60 for holding another branch of the target gas; a gas chromatograph 70 for analyzing the other branch of the target gas held by the collection component; and a controller 50 for controlling the flow paths of the one branch of the target gas and the other branch of the target gas. During thermal analysis, the branching component 20 is controlled to continuously branch the supplied target gas and discharge the one branch of the target gas and the other branch of the target gas, and after the thermal analysis is completed, the other branch of the target gas held by the collection component 60 is supplied to the gas chromatograph 70.

Described herein are various embodiments for the detection of ocular drugs, biomarkers, and small molecules using DNA or RNA aptamers. The DNA or RNA aptamer consists of a binding sequence to selectively and strongly bind to a compound of interest and can be linked to a readily detectable tag. The tag may be fluorescent, radioactive, or chromophore. The detection of the compound of interest can then be accomplished using a simple standard detection method, such as UV-Vis or fluorescence spectrophotometry, or a more complex method, such as mass spectrometry or electrochemical detection, preferably fluorescence detection or radioactivity detection with high sensitivity.

The invention relates to the technical field of biological medicine, and discloses a preparation method and application of a novel spironolactone derivative based on intestinal fungus biotransformation, and the method comprises the following steps: S1, screening an intestinal fungus Syncephalasetum monosporium as a transformation strain; s2, carrying out in-vitro fermentation culture on the screened strains; s3, extracting metabolites in the fermentation liquor; s4, identifying and separating the novel spirolactone derivative by combining mass spectrometry and a chromatographic separation technology; and S5, verifying the aldosterone receptor antagonistic activity of the derivative through molecular docking simulation. A novel spirolactone derivative is identified and separated through strain screening, fermentation condition optimization, multi-stage purification and mass spectrometry and chromatographic separation technologies, the enzyme activity of the strain is activated through shake culture in a culture medium to promote metabolism, the product purity is improved through inactivation, extraction and chromatographic purification, and the problems that a product is single and low in purity in a traditional method are solved.

Processes for quantitating the corrosivity of naphthenic acids in a sample comprising crude oil or a liquid fraction thereof by reacting the sample with a metal comprising iron to produce iron naphthenates that are then stabilized by a ligand. The stabilized iron naphthenates are then analyzed by mass spectrometry to accurately quantitate the percentage of total naphthenic acids in the sample that are iron-reactive naphthenic acids associated with metal corrosion.

The application discloses an analysis method of non-volatile organic acids in electronic cigarette liquid, comprising the following steps: adding a to-be-tested sample of electronic cigarette liquid into an internal standard stock solution and a formic acid-methanol aqueous solution, sealing and oscillating, diluting with an internal standard solution to obtain a to-be-tested sample solution; mixing single standard stock solutions prepared by different target standard samples, diluting into a mixed standard intermediate solution, taking different volumes of the mixed standard intermediate solution, adding an internal standard stock solution and a formic acid-methanol aqueous solution, and configuring into standard working solutions with different concentrations; respectively performing ultra-high performance liquid chromatography-tandem mass spectrometry analysis on the to-be-tested sample solution and the standard working solution; and according to the analysis result, the content of the target in the to-be-tested sample is calculated by using an internal standard standard curve method. The application can realize simultaneous analysis and chromatographic separation of six non-volatile organic acids in electronic cigarette liquid, has the advantages of accurate quantification, high flux, good separation degree, simple operation, high sensitivity, good recovery rate and good repeatability, etc.

The invention relates to the technical field of analytical chemistry sample pretreatment, in particular to an efficient sample injection system for improving liquid sample injection efficiency. Comprising an injection pump, an enrichment cavity, an organic filter membrane, a closed heating pipe and a capillary tube, the organic filter membrane is arranged in the enrichment cavity and divides the enrichment cavity into a chamber I and a chamber II, the injection pump injects a liquid sample into the chamber I through a first pipeline, and the liquid sample is enriched in the chamber II through the organic filter membrane; the chamber II is sequentially connected with a closed heating tube and a capillary tube through a second pipeline, an outlet of the capillary tube is connected with a time-of-flight mass spectrum, the chamber II injects purging gas through a first purging gas pipeline, an enriched sample in the chamber II is purged into the closed heating tube to be gasified and heated, and then the enriched sample is introduced into the time-of-flight mass spectrum through the capillary tube to be analyzed. The device has the characteristics of compact structure, high integration level and high enrichment and gasification efficiency, realizes efficient conversion and sample introduction from a liquid sample to gas, and remarkably improves the sensitivity and efficiency of mass spectrometry.

The present invention relates to a method for highly sensitive in-situ detection of reaction transient intermediates under high pressure, including sampling: obtaining intermediate products and target products in a high-pressure reaction chamber under a pressure difference to obtain a particle beam to be analyzed; photoionization: irradiating the particle beam to be analyzed with extreme ultraviolet light to cause photoionization of the particle beam to be analyzed, obtaining detectable charged ions; mass spectrometry analysis: performing mass spectrometry analysis on the charged ions obtained by photoionization, and obtaining a reaction mechanism according to the mass spectrometry analysis results; the photoionization and the mass spectrometry analysis are both carried out under the condition that the vacuum degree is ≤1×10<supgt;‑8< / supgt; Torr. An apparatus for implementing the method is also disclosed. Compared with traditional methods, the present invention can deeply understand the actual catalytic reaction mechanism and provide a scientific basis for improving the performance of catalysts. The present invention can achieve in-situ characterization of catalytic reactions under normal pressure or even a certain pressure.