54 results about "Desorption ionization" patented technology

The present invention relates to a method for quickly identifying white vaseline from different manufacturers based on silver salt-assisted laser desorption ionization-time of flight mass spectrometry, and belongs to the field of detection. Specifically comprising the following steps: S1, exploring and optimizing metal salt ion conditions, matrix conditions, and sampling methods, and using the optimized experimental conditions for sample preparation; S2, measuring the sample; S3, using eMSTAT Solution software for data analysis to achieve identification of white vaseline from different manufacturers. The method of the present invention replaces traditional organic matrices with silver salts, eliminates background interference, and improves the mass spectrometry signal-to-noise ratio; combines a direct smearing method to avoid complex solvent preparation and extraction, and is easy to operate; applies a mass spectrometer for mass spectrometry acquisition, has accurate molecular weight, reduces the detection time of each sample to 6 seconds, has high analytical throughput, and is low in cost, thereby achieving high-throughput, accurate, and rapid identification of large quantities of white vaseline from different sources.

The application discloses application of 2-amino isophthalic acid (2-AIA) as a MALDI matrix in characterization of oligosaccharide additives in food. The skeleton structure of 2-AIA has a strong ultraviolet absorption group and a protonation site, which is beneficial to laser energy transmission and desorption ionization process of the measured object. Meanwhile, 2-AIA has multiple polar functional groups, which can effectively enhance the directional combination of the hydroxyl group of 2-AIA and oligosaccharide, and improve the anti-interference ability of impurities in a complex system. In addition, 2-AIA and a traditional organic matrix alpha-cyano-4-hydroxycinnamic acid (CHCA) are combined as a binary matrix (CHCA / 2-AIA), which can play a synergistic role of the double matrix, significantly improve the detection sensitivity of oligosaccharide. On the other hand, the combination of CHCA and 2-AIA with different pH values can significantly improve the uniformity of the co-crystallization, and improve the accuracy and reproducibility of quantitative detection.

The invention discloses a preparation method and application of a peptide specific quality nanoprobe. The nanoprobe is prepared by uniformly mixing mass tag molecules, functional polypeptide and a chloroauric acid solution and adding a sodium hydroxide solution through a simple one-pot method. The synthesized mass nanoprobe is specifically recognized and combined with target protein on cells or tissues through a peptide chain, and mass-to-charge ratio signals of mass tag molecules corresponding to different nanoprobes are detected through laser desorption ionization-time-of-flight mass spectrometry (LDI-TOFMS) ionization; the mass spectrum imaging analysis on the relative expression quantity of various proteins on different types of cancer cells and tumor tissue slices is realized. The probe has the characteristics of simple preparation, specific recognition, low background, high resolution and the like, and is easy to popularize to the detection of various proteins on the surfaces of other biological samples.

A novel method for artificial photosynthesis utilizing a chemical system that operates by harnessing visible light and directly capturing carbon dioxide and water from the atmosphere. The system is based on self-organizing complexes comprising visible-light-sensitive chromophores, such as 2-phenyl indole and 2-phenyl benzoxazole, along with titanium tetrachloride, which autonomously perform continuous, complex chemical operations to produce long-chain (C2 to C17) oxygenated organic materials. The process employs earth-abundant metal coordination compounds, including titanium (Ti), zirconium (Zr), hafnium (Hf), and vanadium (V), in the solid state. These compounds form carbonated metal derivatives upon hydrolysis, which are subsequently reduced through proton transfer from water. The system initially generates C1 materials that oligomerize via a novel self-catalyzed mechanism intrinsic to the system's operation. Monitoring and characterization of the chemical transformations have been conducted using high-resolution MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry, supported by infrared (IR) spectroscopy and nuclear magnetic resonance (NMR) analysis. This innovation represents a sustainable and scalable approach for converting atmospheric CO2 into valuable organic materials.

The invention discloses an application of a benzophenone derivative as a bipolar matrix-assisted laser desorption ionization matrix in mass spectrometry detection. The benzophenone derivative matrix provided by the invention can effectively improve desorption ionization efficiency and reduce background interference, is compatible with a positive ion and negative ion bipolar detection mode, and solves the technical bottleneck of single polarity of a traditional matrix detection mode. The matrix has a wide-spectrum absorption characteristic in an ultraviolet region, can be adapted to laser wavelengths of various matrix-assisted laser desorption ionization mass spectrometers, and has a wide application prospect. Besides, when the matrix is used for lipid compound detection, lipid endogenous metabolite spectrum signals with high sensitivity and high coverage rate can be obtained in positive and negative ion modes, the matrix is expected to be widely used for metabonomics and clinical basic research, and key technical support is provided for high-sensitivity and bipolar imaging of small molecule compounds in complex biological samples.

The present application relates to atmospheric pressure ion manipulation technology, specifically, the present application discloses a thermal desorption ionization reaction device, specifically, a thermal desorption ionization reaction device, comprising a stainless steel metal desorption ionization cavity, the metal desorption ionization cavity is a hollow circular cylinder with an open upper end and a closed lower end, an insulating cover for sealing the open upper end is arranged on the open upper end, and a gas and ion outlet is arranged in the middle of the insulating cover; the counter electrode and the electrode with holes are connected to the high-voltage output end of the high-voltage power supply and the ground electrode (or negative electrode) respectively. The voltage applied to the counter electrode and the electrode with holes is alternating current or direct current, and the voltage amplitude is 2000V. The present application improves the consistency and stability of batch production of thermal desorption ionization modules based on integrated design, reduces power consumption and processing cost, and is beneficial to the industrialization of mass spectrometry or ion mobility spectrometry.

The application relates to a gold nanoparticle array preparation method with strong stability and reusability, high cysteamine-stabilized gold nanoparticles are prepared first, the spacing between the gold nanoparticles is changed by utilizing the coulomb repulsion between the gold nanoparticles, then the gold nanoparticle solution is added dropwise to a glass surface modified by gamma-glycidoxypropyltrimethoxysilane to react, stable covalent bonds are formed, and gold nanoparticle arrays with uniform distribution on the glass surface can be obtained after the reaction. The preparation does not require high temperature, the experimental operation is simple and convenient, the gold nanoparticles are arranged in order and closely, have high detection sensitivity, have strong ultraviolet absorption capacity, good stability, reusability, salt resistance and low background interference, and can be reused. The gold nanoparticle array can realize detection analysis on high-salt-content samples in surface-assisted laser desorption ionization mass spectrometry and be used for rapid analysis of small molecule compounds in positive and negative ion dual modes.

The application discloses an ion source device for desorption of americium 241, and relates to the technical field of ion sources.The ion source device mainly comprises americium 241, a clamping device for the americium 241, an R-axis rotating platform and a sample stage.The americium 241 is installed on the clamping device for the americium 241, and the clamping device is installed on the R-axis rotating platform.A glass slide coated with a sample is installed on the sample stage, and the sample stage and the R-axis rotating platform are installed on two XYZ-axis displacement platforms, respectively, and the two XYZ-axis displacement platforms are adjusted to move in the X, Y and Z directions, respectively.The alpha particles generated by the americium 241 decay generate a series of plasmas in the air and are injected into the surface of the sample, so that the molecules on the surface of the sample are ionized and desorbed, and the ions enter a mass spectrometer through a sample inlet for detection.The relative positions of the americium 241, the sample stage and the sample inlet are adjusted, so that the mass spectrometer obtains the best ion signal, and the operation is safe and simple, and the common molecules can be rapidly detected.

The present application relates to the technical field of food microbial detection, and specifically to a method for identifying Daqu, comprising the following steps: (1) weighing a Daqu sample and filtering the Daqu sample to obtain a filtrate; (2) measuring the filtrate, adding magnetic beads to the filtrate, and incubating the filtrate to obtain an incubation solution; (3) removing the magnetic beads from the incubation solution and lysing the microorganisms on the magnetic beads to obtain a sample to be tested; (4) detecting the sample to be tested using matrix-assisted laser desorption ionization time-of-flight mass spectrometry; and identifying the type of Daqu based on characteristic peaks in the mass spectrum of the sample to be tested. The present application has the advantages of accurate detection results and high detection efficiency.

A post-ionization laser module (30) for docking an atmospheric-pressure MALDI ion source (10) comprises a pulsed post-ionization (PI) laser (32), a synchronisation device with a delay generator, for controlling a timing of laser pulses of the post-ionization laser with respect to desorption events in the MALDI ion source, and an optical system (46, 48, 50, 52) for directing the laser pulses on an optical path from the post-ionization laser into a desorption plume (26) of the MALDI ion source. In a further aspect, the invention relates to an AP-MALDI ion source (10), comprising a target holder (12) for mounting a sample target (16), a pulsed desorption laser (14) for irradiating the sample target and creating desorption events, and a post-ionization laser module (30).

The application discloses a method for screening metabolic biomarkers based on saliva metabolic fingerprint and application, and relates to the technical field of biological sample metabolic analysis. The method comprises the following steps: step 1, inorganic nanoparticles are used to enhance laser desorption ionization time-of-flight mass spectrometry technology, saliva samples of Parkinson's disease patients and healthy volunteers are subjected to metabolic detection, and saliva metabolic fingerprints are obtained; step 2, the saliva metabolic fingerprints obtained in step 1 are subjected to a machine learning algorithm, saliva samples are subjected to a variety of model training, a variety of models are evaluated through a cross-validation method, and a final prediction model is screened according to a verification result; step 3, saliva samples of Parkinson's disease patients and healthy volunteers are predicted according to the prediction model of step 2, a prediction result is obtained, and metabolic biomarkers in the saliva samples are screened according to the prediction result. The application has high screening efficiency, the result is accurate and reliable, and the screened metabolic biomarkers can be used for prediction of Parkinson's disease.

The invention belongs to the technical field of analysis and detection, and particularly relates to a preparation method and application of a matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry plant sample, the preparation method of the plant sample created by the invention can separate leaf inner epidermis and mesophyll tissue, and the leaf inner epidermis and mesophyll tissue are utilized to research metabolites in leaves. After the inner epidermis and mesophyll of the leaf are separated, not only can organic acid, small molecular compounds and macromolecular substances in the leaf be detected, but also the change of the content of the organic acid can be revealed. According to the MALDI mass spectrometry technology, assimilation of NH4 < + > / NO3 <-> in leaf inner epidermis or mesophyll tissue can be monitored in an imaging mode, so that the metabolic process of nitrogen is visualized; in the nitrogen metabolism process, NH4 < + > is directly used for synthesizing amino acid, NO3 <-> is reduced into NO2 <->, and NO2 <-> is assimilated into amino acid and derivatives thereof. The preparation method provided by the invention has unique advantages, and is beneficial to deeply understanding the metabolic process and biological functions of plants.

The application discloses a nucleic acid mass spectrometry technology-based method for identifying common respiratory tract infection pathogens in children. The application provides a complete set of primers, including specific primer group 1 and specific primer group 2; the primer group 1 (i.e. the primer corresponding to well1) is composed of single-stranded DNA molecules shown in sequences 1-17 and single-stranded DNA molecules shown in sequences 24-40; the primer group 2 (i.e. the primer corresponding to well2) is composed of single-stranded DNA molecules shown in sequences 18-23 and single-stranded DNA molecules shown in sequences 41-46. The CCRP-MS of the application is a 23-pathogen determination method combining matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and multiple reverse transcription polymerase chain reaction (MRT-PCR), and can directly detect common respiratory tract pathogens in children.

A computer-implemented method for detecting at least one analyte in a sample with a laser desorption mass spectrometer (220) is disclosed. The method comprises:a) at least one imaging step comprising imaging at least one reflective target (128) by using at least one imaging device (235), wherein the sample comprising the at least one analyte is applied to the reflective target (128);b) at least one sample recognition step comprising localizing at least one sample region on the reflective target (128); andc) at least one analyte detection step comprising detecting the at least one analyte in the sample using surface assisted laser desorption ionization mass spectrometry (SALDI-MS) with the laser desorption mass spectrometer (220), wherein laser irradiation is applied to the reflective target (128) by using at least one laser source (222) of the laser desorption mass spectrometer (220) such that at least one ion of the at least one analyte is generated which is detected by using at least one of a mass analyzing unit (224) or an ion-mobility spectrometry device of the laser desorption mass spectrometer (220), wherein the laser irradiation is steered on the localized sample region by using at least one control device (237).

The invention belongs to the field of environmental sample analysis and detection, and particularly relates to a target-oriented synthesized TCBPA molecularly imprinted polymer, a preparation method thereof and a tetrachlorobisphenol A. After glass fibers are cleaned, the surface of the glass fibers is bonded with the TCBPA molecularly imprinted polymer, and an enrichment probe is obtained; the method comprises the following steps: placing an enrichment probe in a to-be-detected sample solution, carrying out vortex adsorption for a set time, washing away a residual matrix on the surface of the enrichment probe after the adsorption is completed, then fixing the enrichment probe at a mass spectrum injection port, and keeping a set distance between the tip of the enrichment probe and the mass spectrum injection port; the charged desorption solution continuously flows onto the enrichment probe to wet the enrichment probe, stable spray is formed at the tip of the enrichment probe, the adsorbed tetrachlorobisphenol A is desorbed and ionized under the combined action of an electric field and the desorption solution, and the obtained ions directly enter a mass spectrometer to be detected. The MIP is directly fixed on the surface of the glass fiber to construct a functional coating capable of being used for continuous flow desorption ionization mass spectrometry detection, so that rapid enrichment and ionization of target molecules are realized.

The invention relates to the technical field of mass spectrum detection, in particular to a metal heterogeneous material, a chip and application of the metal heterogeneous material and the chip in drug mass spectrum analysis. According to the metal heterogeneous material, tight combination of TiO2 and gold is achieved through lattice matching design, and a plasma resonance enhanced heterogeneous interface is constructed. The chip comprises a titanium dioxide nanorod array formed on the substrate, a gold array deposited on the surface of the semiconductor layer and formed by gold nanoparticles, and a surface modification layer. Through the heterostructure design of a semiconductor and gold nanoparticles and a surface modification technology, the laser desorption ionization efficiency and the specific adsorption capacity to drug small molecules are remarkably improved, high-flux, high-sensitivity and high-resolution detection of complex components of traditional drugs is achieved, the gold nanoparticles are applied to drug screening and quality control, and the application prospect is wide. And an efficient analysis tool is provided for traditional drug research.

Disclosed are methods for enhancing a Raman intensity of a sample by adding to the sample an aromatic compound having an absorbance ranging from about 250 nm to about 500 nm, and also, a solvent and a matrix-assisted laser desorption / ionization (MALDI) matrix. Workflows for a combined Raman-MALDI imaging instrument also are disclosed.

This method for analyzing a morpholine ring-containing oligonucleotide is configured such that a morpholine ring-containing oligonucleotide contained in a sample is analyzed with a matrix-assisted laser desorption / ionization mass spectrometer using a mixed matrix containing 3-hydroxypicolinic acid (3-HPA) and 2',4',6'-trihydroxyacetophenone (THAP). This method uses a matrix suitable for the ionization of molecules of a morpholine ring-containing oligonucleotide by the MALDI method, and it is thus possible to more reliably measure the molecular weight of a morpholine ring-containing oligonucleotide in mass spectrometry using the MALDI method.

The invention discloses a method for detecting monosaccharide / oligosaccharide based on 6-aminoquinoxaline assisted laser desorption ionization mass spectrometry, and belongs to the technical field of monosaccharide and oligosaccharide detection methods. A mixed solution of a 6-aminoquinoxaline matrix solution and a to-be-detected sample is dripped on a target plate, and a matrix-assisted laser desorption ionization time-of-flight mass spectrometer is used for mass spectrometry detection for rapid analysis of monosaccharides and oligosaccharides in the sample. The 6-aminoquinoxaline not only can be used as a reactive matrix to efficiently derive monosaccharide and reducing sugar with the polymerization degree of 2-10, but also can remarkably promote the ionization efficiency of non-reducing sugar, and has the advantages of high sensitivity, high universality, low cost, low background interference, uniform crystallization with an analyte and the like; the problems that a traditional organic matrix is uneven in crystallization, and the signal-to-noise ratio of mono / oligosaccharide detection is low are solved. 6-aminoquinoxaline is taken as a matrix, so that high-efficiency detection of monosaccharide and oligosaccharide in a complex sample by an MALDI-MS (matrix-assisted laser desorption ionization-mass spectrometry) method can be realized, and the method has a good application prospect in the fields of medicine, food, medicines and the like.

To enable a user to easily prepare a highly uniform crystal in which a sample and a matrix are mixed in the preparation of the sample in mass spectrometry by a MALDI (matrix-assisted laser desorption ionization) method.SOLUTION: A sample preparation method in mass spectrometry by a MALDI method includes a step S12 of preparing a mixed solution of a liquid containing a compound to be subjected to mass spectrometry and a matrix liquid on a sample plate, and a step S10 and a S14 of adjusting the temperature of the sample plate to a temperature within a preparation range. The temperature in the preparation range is 30-60 °C.SELECTED DRAWING: Figure 4

A computer-implemented method for detecting at least one analyte in a sample with a laser desorption mass spectrometer (220) is disclosed. The method includes: a) at least one imaging step, including imaging at least one reflective target (128) to which a sample containing at least one analyte is applied, using at least one imaging device (235); b) at least one sample recognition step, including locating at least one sample area on the reflective target (128); and c) at least one analyte detection step, including detecting at least one analyte in the sample using surface-assisted laser desorption / ionization mass spectrometry (SALDI-MS) in a laser desorption mass spectrometer (220), wherein laser radiation is applied to the reflective target (128) using at least one laser source (222) of the laser desorption mass spectrometer (220), and the laser radiation is directed to the located sample area using at least one control device (237), such that at least one ion of the at least one analyte is generated, and the ion is detected using at least one of a mass analysis unit (224) of the laser desorption mass spectrometer (220) or an ion mobility spectrometer.

The invention relates to the technical field of clinical blood concentration monitoring and control, and discloses a method for rapidly, accurately and quantitatively detecting the blood concentration of antibiotics, which comprises the following steps: collecting a blood sample containing to-be-detected antibiotics through standard clinical operation (such as venipuncture); the method comprises the following steps: performing protein precipitation treatment on a biological sample containing one or more antibiotics to be detected, and extracting supernate; taking the supernate, applying the supernate to the surface of a solid sample injection carrier, and carrying out drying treatment; placing the solid sample introduction carrier carrying the dry sample between a direct normal pressure ion source and a sample introduction port of a mass spectrometer, and carrying out desorption, ionization and mass spectrometry to obtain mass spectrometry data; and based on the mass spectrum data, calculating to obtain the blood concentration of the to-be-detected antibiotic in the biological sample. By means of the plasma in-situ ion source-mass spectrometry, the accuracy of the mass spectrometry is achieved, rapid and accurate quantitative detection of the plasma concentration can be achieved, and a patient can be treated in time.

The present disclosure provides a kit and its application, the kit comprising two sets of amplification primer groups, single base extension primers and processing reagents; the amplification primer groups are used for amplifying Mycobacterium tuberculosis flora identification sites and anti-tuberculosis drug pharmacogenetic polymorphism sites; each pair of primers corresponds to the upstream and downstream regions of a SNP site; the processing reagents include PCR reaction mixture, PCR enzyme mixture, SAP reaction mixture, SAP enzyme mixture, extension reaction mixture and extension enzyme mixture. By designing specific amplification primer groups and single base extension primers, the system and reagents are optimized, and the sensitivity is high. The kit combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry can complete the detection of all sites, and the detection cost is low and the efficiency is high.

The present invention relates to the field of nanomaterial application technology, and more specifically, to the application of mass spectrometry analysis of water-soluble melanin nanoparticles modified with bifunctional groups. The water-soluble melanin nanoparticles modified with bifunctional groups are MNP-PEG, with a particle size of 4nm to 5nm, uniform particle size, good monodispersity in water, strong ultraviolet absorption ability at 355nm, and an absorbance value of 0.35 to 0.56. In addition, the synthesis process is simple, the preparation cost is low, it is safe, non-toxic, and biodegradable. As a new matrix for matrix-assisted laser desorption ionization time-of-flight mass spectrometry, it can perform efficient mass spectrometry analysis. When detecting tetrabromobisphenol A series molecules, it has the advantages of low background interference peaks, high ion intensity, high sensitivity, etc., and MNP-PEG can also be used for mass spectrometry analysis of small molecules of environmental pollutants in food, which has potential significance for evaluating related health problems caused by the intake of tetrabromobisphenol A and its various derivatives through food.

The invention discloses a method for realizing geographical tracing of prawns by analyzing and identifying intestinal microbial flora of prawns on the basis of mass spectrometry and bacterial library search, which is used for directly analyzing and identifying the intestinal microbial flora of aquatic food. The effectiveness of searching and identifying bacteria by using a Bacteria MS database is evaluated and proved, prawns are used as an aquatic food model, and the intestinal microflora of the Chinese prawns collected from three different aquaculture farms in China is analyzed by using a matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The reliability of the method in the aspect of tracking the geographic source of the aquatic product is further verified by analyzing the samples of the black tiger shrimps and the three crucian carps from three different producing areas, and the rapid, accurate and feasible method can be provided for determining the geographic source of the prawn by analyzing the intestinal microflora of the prawn by utilizing MALDI-TOF MS and combining bacterial library search.

The present invention is directed to a method for rapid analysis aqueous analytes, such as water contaminants, by concentrating analytes on solid adsorbents and then detecting using mass spectrometric analysis via desorption ionization of the contaminant directly from the adsorbent surface. The contaminant may be PFAS. This general approach was demonstrated via indirect solution analysis (ISA) with desorption electrospray ionization (ISA-DESI) and laser desorption ionization (ISA-LDI). These method can use various adsorbent materials, including commercial porous carbons, activated alumina, and metal-organic frame-works (MOFs). As an example, perfluorooctanoic acid (PFOA) was rapidly detected in under five minutes from solutions with PFOA concentrations in the parts-per-trillion (ppt) range. The advantages of this ISA are that sample preparation and analysis are greatly simplified and accelerated relative to conventional methods, the method can maintain extremely low detection limits compared to conventional analysis, and it can be performed with low-cost, readily available materials.

The invention relates to the technical field of ion sources, and discloses a heating type synchronous desorption and ionization integrated plasma ion source which comprises a ceramic body, the ceramic body is fixedly connected to the inner side of a mounting cylinder, a cathode is arranged on the left side of the interior of the mounting cylinder, and a heating module is arranged in the middle of the inner side of the ceramic body. Plasmas are arranged on the inner wall of the mounting cylinder, the heating module is mounted outside the plasmas, a sample table is arranged at the bottom of the right side of the mounting cylinder, and a main body outlet is formed in the bottom of the right side of the mounting cylinder. A wire is connected through the conductive opening to form a closed loop, after voltage is applied between the cathode and the anode and helium in the mounting cylinder is broken down, plasma can be formed on the inner side of the heating module, the plasma can synchronously perform desorption ionization on a sample on the sample table, generated ions are detected through the main body outlet, and the heating module supports real-time temperature adjustment. And moreover, the integrated ceramic main body does not need to replace quick-wear parts.

The application relates to the field of analytical test technology and discloses a desorption ionization source for small mass spectrometry in a low-pressure environment, which comprises a low-pressure desorption cavity, a sample tray, a metal capillary, a discontinuous atmospheric pressure interface, a quartz light window, a halogen lamp, a vacuum gauge, an electromagnetic valve, a gas suction pump, a dopant bottle, a VUV lamp and a stepping motor; the application places a sample in a limited-volume low-pressure environment to perform thermal desorption, reduces neutral particles in the thermal desorption cavity, makes the desorption cavity be full of the sample to be measured after the sample is desorbed, improves ion utilization rate, and further improves instrument sensitivity; meanwhile, the application reduces photon absorption of the VUV lamp by oxygen and water, and improves sample ionization efficiency.

The invention discloses an application of 2-aminoisophthalic acid as an MALDI (matrix-assisted laser desorption ionization) matrix in representing an oligosaccharide additive in food. The skeleton structure of the 2-AIA has a strong ultraviolet absorption group and a protonation site, which is beneficial to the transmission of laser energy and the desorption ionization process of an object to be detected. Meanwhile, the 2-AIA has a plurality of polar functional groups, so that the directional combination of the 2-AIA and hydroxyl of oligosaccharide can be effectively enhanced, and the impurity interference resistance in a complex system is improved. Besides, the compound is combined with a traditional organic matrix alpha-cyano-4-hydroxycinnamic acid (CHCA) to serve as a binary matrix (CHCA / 2-AIA), on one hand, the synergistic effect of double matrixes can be exerted, and the detection sensitivity of oligosaccharide is remarkably improved. On the other hand, through combined use of CHCA and 2-AIA with different pH values, the uniformity of co-crystallization can be remarkably improved, and the accuracy and reproducibility of quantitative detection are improved.

The invention provides a plasma desorption ionization source system, a plasma desorption ionization source method and a mass spectrometry system, and belongs to the field of mass spectrometry. The plasma desorption ionization source system comprises a low-voltage power supply which provides low-voltage energy for generation of arc plasma; the energy adjusting module is used for adjusting the energy output time and period of the low voltage; the boosting module is used for converting low voltage into high voltage; the electrode needle is connected with one output end of the boosting module; the electrode plate is connected with the other output end of the boosting module and is used for bearing a sample to be tested; the electrode needle serves as a cathode or an anode and discharges with the electrode plate to generate plasma for ionizing a sample; when the to-be-detected sample is non-polar or weak-polar, increasing the energy output time; and when the to-be-tested sample has strong polarity, the energy output time is reduced. According to the method, the limitation of a single mode of a traditional ionization method is broken through, different detection requirements are met by adjusting energy parameters, and the flexibility and accuracy of mass spectrometry are remarkably improved.