39 results about "Desorption ionization" patented technology

The application discloses application of 2-amino isophthalic acid (2-AIA) as a MALDI matrix in characterization of oligosaccharide additives in food. The skeleton structure of 2-AIA has a strong ultraviolet absorption group and a protonation site, which is beneficial to laser energy transmission and desorption ionization process of the measured object. Meanwhile, 2-AIA has multiple polar functional groups, which can effectively enhance the directional combination of the hydroxyl group of 2-AIA and oligosaccharide, and improve the anti-interference ability of impurities in a complex system. In addition, 2-AIA and a traditional organic matrix alpha-cyano-4-hydroxycinnamic acid (CHCA) are combined as a binary matrix (CHCA / 2-AIA), which can play a synergistic role of the double matrix, significantly improve the detection sensitivity of oligosaccharide. On the other hand, the combination of CHCA and 2-AIA with different pH values can significantly improve the uniformity of the co-crystallization, and improve the accuracy and reproducibility of quantitative detection.

The invention discloses a preparation method and application of a peptide specific quality nanoprobe. The nanoprobe is prepared by uniformly mixing mass tag molecules, functional polypeptide and a chloroauric acid solution and adding a sodium hydroxide solution through a simple one-pot method. The synthesized mass nanoprobe is specifically recognized and combined with target protein on cells or tissues through a peptide chain, and mass-to-charge ratio signals of mass tag molecules corresponding to different nanoprobes are detected through laser desorption ionization-time-of-flight mass spectrometry (LDI-TOFMS) ionization; the mass spectrum imaging analysis on the relative expression quantity of various proteins on different types of cancer cells and tumor tissue slices is realized. The probe has the characteristics of simple preparation, specific recognition, low background, high resolution and the like, and is easy to popularize to the detection of various proteins on the surfaces of other biological samples.

A novel method for artificial photosynthesis utilizing a chemical system that operates by harnessing visible light and directly capturing carbon dioxide and water from the atmosphere. The system is based on self-organizing complexes comprising visible-light-sensitive chromophores, such as 2-phenyl indole and 2-phenyl benzoxazole, along with titanium tetrachloride, which autonomously perform continuous, complex chemical operations to produce long-chain (C2 to C17) oxygenated organic materials. The process employs earth-abundant metal coordination compounds, including titanium (Ti), zirconium (Zr), hafnium (Hf), and vanadium (V), in the solid state. These compounds form carbonated metal derivatives upon hydrolysis, which are subsequently reduced through proton transfer from water. The system initially generates C1 materials that oligomerize via a novel self-catalyzed mechanism intrinsic to the system's operation. Monitoring and characterization of the chemical transformations have been conducted using high-resolution MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry, supported by infrared (IR) spectroscopy and nuclear magnetic resonance (NMR) analysis. This innovation represents a sustainable and scalable approach for converting atmospheric CO2 into valuable organic materials.

The present application relates to atmospheric pressure ion manipulation technology, specifically, the present application discloses a thermal desorption ionization reaction device, specifically, a thermal desorption ionization reaction device, comprising a stainless steel metal desorption ionization cavity, the metal desorption ionization cavity is a hollow circular cylinder with an open upper end and a closed lower end, an insulating cover for sealing the open upper end is arranged on the open upper end, and a gas and ion outlet is arranged in the middle of the insulating cover; the counter electrode and the electrode with holes are connected to the high-voltage output end of the high-voltage power supply and the ground electrode (or negative electrode) respectively. The voltage applied to the counter electrode and the electrode with holes is alternating current or direct current, and the voltage amplitude is 2000V. The present application improves the consistency and stability of batch production of thermal desorption ionization modules based on integrated design, reduces power consumption and processing cost, and is beneficial to the industrialization of mass spectrometry or ion mobility spectrometry.

The application relates to a gold nanoparticle array preparation method with strong stability and reusability, high cysteamine-stabilized gold nanoparticles are prepared first, the spacing between the gold nanoparticles is changed by utilizing the coulomb repulsion between the gold nanoparticles, then the gold nanoparticle solution is added dropwise to a glass surface modified by gamma-glycidoxypropyltrimethoxysilane to react, stable covalent bonds are formed, and gold nanoparticle arrays with uniform distribution on the glass surface can be obtained after the reaction. The preparation does not require high temperature, the experimental operation is simple and convenient, the gold nanoparticles are arranged in order and closely, have high detection sensitivity, have strong ultraviolet absorption capacity, good stability, reusability, salt resistance and low background interference, and can be reused. The gold nanoparticle array can realize detection analysis on high-salt-content samples in surface-assisted laser desorption ionization mass spectrometry and be used for rapid analysis of small molecule compounds in positive and negative ion dual modes.

The application discloses an ion source device for desorption of americium 241, and relates to the technical field of ion sources.The ion source device mainly comprises americium 241, a clamping device for the americium 241, an R-axis rotating platform and a sample stage.The americium 241 is installed on the clamping device for the americium 241, and the clamping device is installed on the R-axis rotating platform.A glass slide coated with a sample is installed on the sample stage, and the sample stage and the R-axis rotating platform are installed on two XYZ-axis displacement platforms, respectively, and the two XYZ-axis displacement platforms are adjusted to move in the X, Y and Z directions, respectively.The alpha particles generated by the americium 241 decay generate a series of plasmas in the air and are injected into the surface of the sample, so that the molecules on the surface of the sample are ionized and desorbed, and the ions enter a mass spectrometer through a sample inlet for detection.The relative positions of the americium 241, the sample stage and the sample inlet are adjusted, so that the mass spectrometer obtains the best ion signal, and the operation is safe and simple, and the common molecules can be rapidly detected.

The application discloses a method for screening metabolic biomarkers based on saliva metabolic fingerprint and application, and relates to the technical field of biological sample metabolic analysis. The method comprises the following steps: step 1, inorganic nanoparticles are used to enhance laser desorption ionization time-of-flight mass spectrometry technology, saliva samples of Parkinson's disease patients and healthy volunteers are subjected to metabolic detection, and saliva metabolic fingerprints are obtained; step 2, the saliva metabolic fingerprints obtained in step 1 are subjected to a machine learning algorithm, saliva samples are subjected to a variety of model training, a variety of models are evaluated through a cross-validation method, and a final prediction model is screened according to a verification result; step 3, saliva samples of Parkinson's disease patients and healthy volunteers are predicted according to the prediction model of step 2, a prediction result is obtained, and metabolic biomarkers in the saliva samples are screened according to the prediction result. The application has high screening efficiency, the result is accurate and reliable, and the screened metabolic biomarkers can be used for prediction of Parkinson's disease.

The invention belongs to the technical field of analysis and detection, and particularly relates to a preparation method and application of a matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry plant sample, the preparation method of the plant sample created by the invention can separate leaf inner epidermis and mesophyll tissue, and the leaf inner epidermis and mesophyll tissue are utilized to research metabolites in leaves. After the inner epidermis and mesophyll of the leaf are separated, not only can organic acid, small molecular compounds and macromolecular substances in the leaf be detected, but also the change of the content of the organic acid can be revealed. According to the MALDI mass spectrometry technology, assimilation of NH4 < + > / NO3 <-> in leaf inner epidermis or mesophyll tissue can be monitored in an imaging mode, so that the metabolic process of nitrogen is visualized; in the nitrogen metabolism process, NH4 < + > is directly used for synthesizing amino acid, NO3 <-> is reduced into NO2 <->, and NO2 <-> is assimilated into amino acid and derivatives thereof. The preparation method provided by the invention has unique advantages, and is beneficial to deeply understanding the metabolic process and biological functions of plants.

The application discloses a nucleic acid mass spectrometry technology-based method for identifying common respiratory tract infection pathogens in children. The application provides a complete set of primers, including specific primer group 1 and specific primer group 2; the primer group 1 (i.e. the primer corresponding to well1) is composed of single-stranded DNA molecules shown in sequences 1-17 and single-stranded DNA molecules shown in sequences 24-40; the primer group 2 (i.e. the primer corresponding to well2) is composed of single-stranded DNA molecules shown in sequences 18-23 and single-stranded DNA molecules shown in sequences 41-46. The CCRP-MS of the application is a 23-pathogen determination method combining matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and multiple reverse transcription polymerase chain reaction (MRT-PCR), and can directly detect common respiratory tract pathogens in children.

The invention belongs to the field of environmental sample analysis and detection, and particularly relates to a target-oriented synthesized TCBPA molecularly imprinted polymer, a preparation method thereof and a tetrachlorobisphenol A. After glass fibers are cleaned, the surface of the glass fibers is bonded with the TCBPA molecularly imprinted polymer, and an enrichment probe is obtained; the method comprises the following steps: placing an enrichment probe in a to-be-detected sample solution, carrying out vortex adsorption for a set time, washing away a residual matrix on the surface of the enrichment probe after the adsorption is completed, then fixing the enrichment probe at a mass spectrum injection port, and keeping a set distance between the tip of the enrichment probe and the mass spectrum injection port; the charged desorption solution continuously flows onto the enrichment probe to wet the enrichment probe, stable spray is formed at the tip of the enrichment probe, the adsorbed tetrachlorobisphenol A is desorbed and ionized under the combined action of an electric field and the desorption solution, and the obtained ions directly enter a mass spectrometer to be detected. The MIP is directly fixed on the surface of the glass fiber to construct a functional coating capable of being used for continuous flow desorption ionization mass spectrometry detection, so that rapid enrichment and ionization of target molecules are realized.

The invention relates to the technical field of mass spectrum detection, in particular to a metal heterogeneous material, a chip and application of the metal heterogeneous material and the chip in drug mass spectrum analysis. According to the metal heterogeneous material, tight combination of TiO2 and gold is achieved through lattice matching design, and a plasma resonance enhanced heterogeneous interface is constructed. The chip comprises a titanium dioxide nanorod array formed on the substrate, a gold array deposited on the surface of the semiconductor layer and formed by gold nanoparticles, and a surface modification layer. Through the heterostructure design of a semiconductor and gold nanoparticles and a surface modification technology, the laser desorption ionization efficiency and the specific adsorption capacity to drug small molecules are remarkably improved, high-flux, high-sensitivity and high-resolution detection of complex components of traditional drugs is achieved, the gold nanoparticles are applied to drug screening and quality control, and the application prospect is wide. And an efficient analysis tool is provided for traditional drug research.

Disclosed are methods for enhancing a Raman intensity of a sample by adding to the sample an aromatic compound having an absorbance ranging from about 250 nm to about 500 nm, and also, a solvent and a matrix-assisted laser desorption / ionization (MALDI) matrix. Workflows for a combined Raman-MALDI imaging instrument also are disclosed.

This method for analyzing a morpholine ring-containing oligonucleotide is configured such that a morpholine ring-containing oligonucleotide contained in a sample is analyzed with a matrix-assisted laser desorption / ionization mass spectrometer using a mixed matrix containing 3-hydroxypicolinic acid (3-HPA) and 2',4',6'-trihydroxyacetophenone (THAP). This method uses a matrix suitable for the ionization of molecules of a morpholine ring-containing oligonucleotide by the MALDI method, and it is thus possible to more reliably measure the molecular weight of a morpholine ring-containing oligonucleotide in mass spectrometry using the MALDI method.

The invention discloses a method for detecting monosaccharide / oligosaccharide based on 6-aminoquinoxaline assisted laser desorption ionization mass spectrometry, and belongs to the technical field of monosaccharide and oligosaccharide detection methods. A mixed solution of a 6-aminoquinoxaline matrix solution and a to-be-detected sample is dripped on a target plate, and a matrix-assisted laser desorption ionization time-of-flight mass spectrometer is used for mass spectrometry detection for rapid analysis of monosaccharides and oligosaccharides in the sample. The 6-aminoquinoxaline not only can be used as a reactive matrix to efficiently derive monosaccharide and reducing sugar with the polymerization degree of 2-10, but also can remarkably promote the ionization efficiency of non-reducing sugar, and has the advantages of high sensitivity, high universality, low cost, low background interference, uniform crystallization with an analyte and the like; the problems that a traditional organic matrix is uneven in crystallization, and the signal-to-noise ratio of mono / oligosaccharide detection is low are solved. 6-aminoquinoxaline is taken as a matrix, so that high-efficiency detection of monosaccharide and oligosaccharide in a complex sample by an MALDI-MS (matrix-assisted laser desorption ionization-mass spectrometry) method can be realized, and the method has a good application prospect in the fields of medicine, food, medicines and the like.

To enable a user to easily prepare a highly uniform crystal in which a sample and a matrix are mixed in the preparation of the sample in mass spectrometry by a MALDI (matrix-assisted laser desorption ionization) method.SOLUTION: A sample preparation method in mass spectrometry by a MALDI method includes a step S12 of preparing a mixed solution of a liquid containing a compound to be subjected to mass spectrometry and a matrix liquid on a sample plate, and a step S10 and a S14 of adjusting the temperature of the sample plate to a temperature within a preparation range. The temperature in the preparation range is 30-60 °C.SELECTED DRAWING: Figure 4

A computer-implemented method for detecting at least one analyte in a sample with a laser desorption mass spectrometer (220) is disclosed. The method includes: a) at least one imaging step, including imaging at least one reflective target (128) to which a sample containing at least one analyte is applied, using at least one imaging device (235); b) at least one sample recognition step, including locating at least one sample area on the reflective target (128); and c) at least one analyte detection step, including detecting at least one analyte in the sample using surface-assisted laser desorption / ionization mass spectrometry (SALDI-MS) in a laser desorption mass spectrometer (220), wherein laser radiation is applied to the reflective target (128) using at least one laser source (222) of the laser desorption mass spectrometer (220), and the laser radiation is directed to the located sample area using at least one control device (237), such that at least one ion of the at least one analyte is generated, and the ion is detected using at least one of a mass analysis unit (224) of the laser desorption mass spectrometer (220) or an ion mobility spectrometer.

The invention relates to the technical field of clinical blood concentration monitoring and control, and discloses a method for rapidly, accurately and quantitatively detecting the blood concentration of antibiotics, which comprises the following steps: collecting a blood sample containing to-be-detected antibiotics through standard clinical operation (such as venipuncture); the method comprises the following steps: performing protein precipitation treatment on a biological sample containing one or more antibiotics to be detected, and extracting supernate; taking the supernate, applying the supernate to the surface of a solid sample injection carrier, and carrying out drying treatment; placing the solid sample introduction carrier carrying the dry sample between a direct normal pressure ion source and a sample introduction port of a mass spectrometer, and carrying out desorption, ionization and mass spectrometry to obtain mass spectrometry data; and based on the mass spectrum data, calculating to obtain the blood concentration of the to-be-detected antibiotic in the biological sample. By means of the plasma in-situ ion source-mass spectrometry, the accuracy of the mass spectrometry is achieved, rapid and accurate quantitative detection of the plasma concentration can be achieved, and a patient can be treated in time.

The present disclosure provides a kit and its application, the kit comprising two sets of amplification primer groups, single base extension primers and processing reagents; the amplification primer groups are used for amplifying Mycobacterium tuberculosis flora identification sites and anti-tuberculosis drug pharmacogenetic polymorphism sites; each pair of primers corresponds to the upstream and downstream regions of a SNP site; the processing reagents include PCR reaction mixture, PCR enzyme mixture, SAP reaction mixture, SAP enzyme mixture, extension reaction mixture and extension enzyme mixture. By designing specific amplification primer groups and single base extension primers, the system and reagents are optimized, and the sensitivity is high. The kit combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry can complete the detection of all sites, and the detection cost is low and the efficiency is high.

The present invention is directed to a method for rapid analysis aqueous analytes, such as water contaminants, by concentrating analytes on solid adsorbents and then detecting using mass spectrometric analysis via desorption ionization of the contaminant directly from the adsorbent surface. The contaminant may be PFAS. This general approach was demonstrated via indirect solution analysis (ISA) with desorption electrospray ionization (ISA-DESI) and laser desorption ionization (ISA-LDI). These method can use various adsorbent materials, including commercial porous carbons, activated alumina, and metal-organic frame-works (MOFs). As an example, perfluorooctanoic acid (PFOA) was rapidly detected in under five minutes from solutions with PFOA concentrations in the parts-per-trillion (ppt) range. The advantages of this ISA are that sample preparation and analysis are greatly simplified and accelerated relative to conventional methods, the method can maintain extremely low detection limits compared to conventional analysis, and it can be performed with low-cost, readily available materials.

The invention relates to the technical field of ion sources, and discloses a heating type synchronous desorption and ionization integrated plasma ion source which comprises a ceramic body, the ceramic body is fixedly connected to the inner side of a mounting cylinder, a cathode is arranged on the left side of the interior of the mounting cylinder, and a heating module is arranged in the middle of the inner side of the ceramic body. Plasmas are arranged on the inner wall of the mounting cylinder, the heating module is mounted outside the plasmas, a sample table is arranged at the bottom of the right side of the mounting cylinder, and a main body outlet is formed in the bottom of the right side of the mounting cylinder. A wire is connected through the conductive opening to form a closed loop, after voltage is applied between the cathode and the anode and helium in the mounting cylinder is broken down, plasma can be formed on the inner side of the heating module, the plasma can synchronously perform desorption ionization on a sample on the sample table, generated ions are detected through the main body outlet, and the heating module supports real-time temperature adjustment. And moreover, the integrated ceramic main body does not need to replace quick-wear parts.

The application relates to the field of analytical test technology and discloses a desorption ionization source for small mass spectrometry in a low-pressure environment, which comprises a low-pressure desorption cavity, a sample tray, a metal capillary, a discontinuous atmospheric pressure interface, a quartz light window, a halogen lamp, a vacuum gauge, an electromagnetic valve, a gas suction pump, a dopant bottle, a VUV lamp and a stepping motor; the application places a sample in a limited-volume low-pressure environment to perform thermal desorption, reduces neutral particles in the thermal desorption cavity, makes the desorption cavity be full of the sample to be measured after the sample is desorbed, improves ion utilization rate, and further improves instrument sensitivity; meanwhile, the application reduces photon absorption of the VUV lamp by oxygen and water, and improves sample ionization efficiency.

The invention provides a plasma desorption ionization source system, a plasma desorption ionization source method and a mass spectrometry system, and belongs to the field of mass spectrometry. The plasma desorption ionization source system comprises a low-voltage power supply which provides low-voltage energy for generation of arc plasma; the energy adjusting module is used for adjusting the energy output time and period of the low voltage; the boosting module is used for converting low voltage into high voltage; the electrode needle is connected with one output end of the boosting module; the electrode plate is connected with the other output end of the boosting module and is used for bearing a sample to be tested; the electrode needle serves as a cathode or an anode and discharges with the electrode plate to generate plasma for ionizing a sample; when the to-be-detected sample is non-polar or weak-polar, increasing the energy output time; and when the to-be-tested sample has strong polarity, the energy output time is reduced. According to the method, the limitation of a single mode of a traditional ionization method is broken through, different detection requirements are met by adjusting energy parameters, and the flexibility and accuracy of mass spectrometry are remarkably improved.

The present application relates to atmospheric pressure ion manipulation technology, specifically, the present application discloses a kind of detection method of prohibited compound in complex matrix, realize the removal of solvent and low-boiling point matrix in liquid sample by pre-heating pretreatment;Using quadrupole mass spectrometer equipped with thermal desorption sampler and atmospheric ion source as detection instrument;1-5 μL drop of liquid sample to be measured is added on sample sheet, at the temperature of pre-heating 50-280 DEG C (preferably 160-180 DEG C) processing time 10 seconds to 10 minutes (preferably 4-6 minutes), realize the rapid volatilization of solvent and low-boiling point matrix in liquid sample;Finally, sample sheet is inserted into thermal desorption ionization cavity for gasification and ionization, start mass spectrum scanning, sample mass spectrum can be obtained;The result of the above detection is compared with the characteristic spectrum library of common toxicant of quadrupole mass spectrometer to determine whether prohibited compound is contained in beverage, electronic cigarette oil or not.The present application improves selectivity and identification accuracy.

The invention discloses a method for rapidly detecting polypeptide in cosmetics based on an MALDI-TOF-MS. The method comprises the following steps: S1, weighing a cosmetic sample, adding the cosmetic sample into an extraction solvent, carrying out vortex treatment, then carrying out centrifugal treatment, standing, taking supernate, and enabling the supernate to pass through a 3k ultrafiltration tube, so as to obtain a to-be-detected sample solution; and S2, detecting and analyzing the sample solution to be detected by using matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The MALDI-TOF-MS technology is systematically introduced into the field of cosmetic polypeptide detection for the first time. According to the method, the polypeptide component can be directly and rapidly identified only through simple pretreatment. Compared with a traditional LC-MS / MS method, the method has the advantages that the analysis time can be shortened from tens of minutes to several minutes, the flux is improved by more than ten times, and the efficiency of product quality control and production on-line monitoring is greatly improved.

This invention provides a novel mass spectrometry composite desorption-ionization source device and its application. The device includes a microwave plasma ionization source and an electrospray ionization source. In the microwave plasma ionization source, the variable-diameter dielectric tube in the T-shaped insulating dielectric tube and the electrospray capillary are coaxially arranged and simultaneously introduced with a working coil. One end of the working coil is connected to a microwave introduction unit, and the other end is wound around the thin section of the dielectric tube and back to the microwave introduction unit, and positioned close to the sample to be tested. Thus, in the same spacetime, the electrospray ionization source provides relatively stable initial ionization, and the microwave plasma ionization source further enhances the analytical signal through secondary ionization. Combining the two solves the problem of Coulomb interference, and under the action of the working coil, the ionization region is more concentrated, greatly improving the detection signal by up to 100 times.

An apparatus to generate ions from sample material 8 deposited on a substrate 6 which is at least partially transparent to electromagnetic waves, comprising: a support device 2 which has a holder 4 fo

The invention discloses a novel Cu-based binary metal sulfide nanoflower matrix material, the chemical formula of the novel Cu-based binary metal sulfide nanoflower matrix material is CuXS, and X is selected from Cr, Ni, Co or Fe. The novel Cu-based binary metal sulfide nanoflower matrix material disclosed by the invention has high photoelectric conversion performance and photo-thermal conversion capability, integrates the advantages of two components, and enhances the metabolite desorption / ionization efficiency. The desorption / ionization efficiency of metabolites can be improved, so that the mass spectrometry detection performance is more excellent.

The invention relates to the technical field of biological detection, in particular to a multistage hydrangea heterogeneous oxide as well as a preparation method and application thereof. The multi-stage hydrangea heterogeneous oxide comprises a cobalt-nickel bimetallic oxide and a silicon dioxide template, the bimetallic oxide has more active sites and a synergistic effect, so that charge transfer is facilitated, and the detection sensitivity is improved; the cobalt-nickel bimetallic oxide has a three-dimensional hierarchical pore structure, a bimetallic synergistic effect and interface engineering characteristics, can further improve the desorption / ionization efficiency, the photothermal conversion capability and the structural stability of an analyte, and is an ideal LDI analysis matrix; the silicon dioxide template can accurately regulate and control the morphology, the pore structure and the interface of the material through space limitation, inhibit particle aggregation, enhance the structural stability and guide uniform nucleation; the formed three-dimensional porous framework promotes continuous transmission of charges, the exposure degree of active sites is increased, and then the ionization efficiency is improved.

The invention relates to the technical field of mass spectrometry, in particular to a matrix solution for MALDI (matrix-assisted laser desorption ionization) mass spectrometry imaging as well as a rapid preparation method and application of the matrix solution. The rapid preparation method comprises the following steps: selecting at least one matrix compound suitable for MALDI-MS, and dissolving in a solvent to prepare a matrix solution; the solvent comprises a matrix dissolving component, a high volatile component and an embedding component. When a tissue sample is pretreated, a matrix solution is put into an embedding mold to be cured, the matrix solution is put into a cavity of a slicing machine to be balanced, the slice thickness is adjusted, and the matrix with the complete slice is embedded and pasted on the surface of the tissue sample; and heating in a microwave oven until the solvent is completely volatilized, and carrying out MALDI-MSI analysis. According to the matrix solution provided by the invention, the matrix preparation and coating time can be remarkably shortened, and the uniformity and the crystallization fineness of a coating are improved, so that the quality, the sensitivity and the reproducibility of an MALDI-MSI image are improved.

An apparatus to generate ions from sample material deposited on a substrate which is at least partially transparent to electromagnetic waves, comprises: —a support device having a holder for the substrate, —a desorption / ionization unit including a desorption device and an ionization device, said desorption device being configured to desorb deposited sample material from a desorption site on the substrate using at least one energy burst, and said ionization device being configured to irradiate the desorbed sample material above the substrate with electromagnetic waves after the at least one energy burst, wherein the electromagnetic waves pass through the substrate before encountering the desorbed sample material at a location which corresponds to the desorption site, and—an extraction device which is arranged and designed to extract ions from the desorbed sample material and transfer them into an analyzer. The invention also relates to a correspondingly arranged method.