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Graphene matrix and application of graphene matrix in matrix-assisted laser desorption/ionization-time of flight-mass spectrometry detection

A matrix-assisted laser and time-of-flight mass spectrometry technology, which is applied in the field of analysis and detection, can solve the problems of difficult sample analysis, time-consuming and labor-intensive, limited monitoring, etc., and achieves the effects of strong versatility, high wear resistance and low cost.

Inactive Publication Date: 2012-04-25
程金生
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the actual analysis of samples, the sources of samples are very complex systems such as blood, cell lysates, tissues, culture fluids, etc., which contain a large amount of impurities such as proteins, salts, surfactants, preservatives, etc. In addition, the sample itself Hydrophobicity and acid-base properties also greatly affect ionization efficiency, so traditional matrices (such as α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), sinapinic acid (SA)) MALDI-TOF MS is difficult to analyze such a complex sample, and it also has high requirements for the operator, and the operator needs a long time to find the best experimental conditions, which is time-consuming and laborious
Therefore, the analysis of low-molecular-weight compounds and the monitoring of biocatalytic reaction systems such as small-molecule metabolites are limited by this technology, and the development of new substrates is urgently needed.
[0006]The application of graphene matrix in MALDI-TOF MS mass spectrometry has not been reported yet

Method used

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  • Graphene matrix and application of graphene matrix in matrix-assisted laser desorption/ionization-time of flight-mass spectrometry detection
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  • Graphene matrix and application of graphene matrix in matrix-assisted laser desorption/ionization-time of flight-mass spectrometry detection

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Experimental program
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Effect test

Embodiment 1

[0064] Embodiment 1: Method for detecting mixed amine samples such as spermine, spermidine, putrescine, etc.

[0065] (1) The instruments and reagents are: BIFLEX III MALDI TOF mass spectrometer (Bruker Company); nitrogen laser with a wavelength of 337 nm, adopting the working mode of delayed extraction and reflection of ions, positive ion detection; the collected The data were processed with Flexcontrol 2.2, data analysis software Flex Analysis 2.4, mass spectrometry imaging software Flex Imaging 1.0, differential analysis software Clin Protools 2.0 and other software;

[0066] (2) Prepare a graphene nanosheet coating on the carrier, and dry it with gas for later use;

[0067] (3) Immobilize the carrier on the MALDI target;

[0068] (4) After the sample solution of mixed amines (equal molar ratio) such as spermine, spermidine, putrescine, etc. dispersed in N,N-dimethylformamide solvent is added dropwise to the surface of the graphene film or coating, the sample and the matri...

Embodiment 2

[0069] Embodiment 2: the method for detecting annulene sample

[0070] (1) The instruments and reagents are: BIFLEX III MALDI TOF mass spectrometer (Bruker Company); nitrogen laser with a wavelength of 337 nm, adopting the working mode of delayed extraction and reflection of ions, positive ion detection; the collected The data were processed with Flexcontrol 2.2, data analysis software Flex Analysis 2.4, mass spectrometry imaging software Flex Imaging 1.0, differential analysis software Clin Protools 2.0 and other software;

[0071] (2) Prepare a graphene nanosheet coating on the carrier, and dry it with gas for later use;

[0072] (3) Immobilize the carrier on the MALDI target;

[0073] (4) After adding the annulene sample solution dispersed in xylene solvent onto the surface of the graphene coating, the mixed crystals formed by the sample and the matrix are secondary crystallized, and the sample to be analyzed is prepared after the solvent evaporates, and the laser analysis...

Embodiment 3

[0074] Embodiment 3: the method for detecting squalene sample

[0075] (1) The instruments and reagents are: BIFLEX III MALDI TOF mass spectrometer (Bruker Company); nitrogen laser with a wavelength of 337 nm, adopting the working mode of delayed extraction and reflection of ions, positive ion detection; the collected The data were processed with Flexcontrol 2.2, data analysis software Flex Analysis 2.4, mass spectrometry imaging software Flex Imaging 1.0, differential analysis software Clin Protools 2.0 and other software;

[0076] (2) Prepare a graphene nanosheet coating on the carrier, and dry it with gas for later use;

[0077] (3) Immobilize the carrier on the MALDI target;

[0078] (4) After the squalene sample solution is dispersed in N,N-dimethylformamide solvent and added dropwise to the surface of the graphene film or coating, the mixed crystal formed by the sample and the matrix is ​​secondary crystallized, and the solvent is volatilized. The samples to be analyzed ...

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Abstract

The present invention discloses a graphene matrix and an application of the graphene matrix in matrix-assisted laser desorption / ionization-time of flight-mass spectrometry (MALDI-TOF-MS) detection. According to the present invention, in the MALDI-TOF-MS, the graphene is adopted as an assisted matrix, such that the analysis and the detection of the materials including from the small molecule compounds to the biological macromolecules can be efficiently and rapidly achieved; importantly the background interference of the molecular ion peak of the traditional organic matrix can be effectively eliminated so as to achieve the analysis and the detections of amino acids, lipid compounds, peptides, proteins, oligonucleotides, and other structural molecules; the MALDI-TOF-MS adopting the graphene as the matrix is the desorption ionization method, which does not require addition of any organic matrixes, such that the decomposition of the analyte can be avoided, and good reproducibility and high salt tolerance are provided; the efficient and rapid detection method is provided for the natural products and the biological metabolites; the method further can be used for the detection of the common biological macromolecules, and can be popularized and applied in all the MALDI-TOF mass spectrometry so as to achieve the deep application of the graphene.

Description

technical field [0001] The invention relates to analysis and detection, in particular to a graphene matrix and its application in matrix-assisted laser desorption ionization time-of-flight mass spectrometry detection. Background technique [0002] Matrix-assisted laser desorption ionization time-of-flight mass spectrometry is a new type of soft ionization biological mass spectrometry developed in recent years, which is very simple and efficient both in theory and in design. The instrument mainly consists of two parts: matrix-assisted laser desorption ionization source (MALDI) and time-of-flight mass analyzer (TOF). The principle of MALDI is to irradiate the co-crystal film formed by the sample and the matrix with laser light. The matrix absorbs energy from the laser and transmits it to the biomolecules. During the ionization process, the protons are transferred to the biomolecules or protons are obtained from the biomolecules, so that the biomolecules are ionized. process. ...

Claims

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Application Information

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IPC IPC(8): G01N27/64G01N1/34G01N1/38
Inventor 程金生
Owner 程金生
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