Graphene matrix and application of graphene matrix in matrix-assisted laser desorption/ionization-time of flight-mass spectrometry detection

Abstract

The present invention discloses a graphene matrix and an application of the graphene matrix in matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) detection. According to the present invention, in the MALDI-TOF-MS, the graphene is adopted as an assisted matrix, such that the analysis and the detection of the materials including from the small molecule compounds to the biological macromolecules can be efficiently and rapidly achieved; importantly the background interference of the molecular ion peak of the traditional organic matrix can be effectively eliminated so as to achieve the analysis and the detections of amino acids, lipid compounds, peptides, proteins, oligonucleotides, and other structural molecules; the MALDI-TOF-MS adopting the graphene as the matrix is the desorption ionization method, which does not require addition of any organic matrixes, such that the decomposition of the analyte can be avoided, and good reproducibility and high salt tolerance are provided; the efficient and rapid detection method is provided for the natural products and the biological metabolites; the method further can be used for the detection of the common biological macromolecules, and can be popularized and applied in all the MALDI-TOF mass spectrometry so as to achieve the deep application of the graphene.

Description

technical field [0001] The invention relates to analysis and detection, in particular to a graphene matrix and its application in matrix-assisted laser desorption ionization time-of-flight mass spectrometry detection. Background technique [0002] Matrix-assisted laser desorption ionization time-of-flight mass spectrometry is a new type of soft ionization biological mass spectrometry developed in recent years, which is very simple and efficient both in theory and in design. The instrument mainly consists of two parts: matrix-assisted laser desorption ionization source (MALDI) and time-of-flight mass analyzer (TOF). The principle of MALDI is to irradiate the co-crystal film formed by the sample and the matrix with laser light. The matrix absorbs energy from the laser and transmits it to the biomolecules. During the ionization process, the protons are transferred to the biomolecules or protons are obtained from the biomolecules, so that the biomolecules are ionized. process. ...

AI Technical Summary

Problems solved by technology

However, in the actual analysis of samples, the sources of samples are very complex systems such as blood, cell lysates, tissues, culture fluids, etc., which contain a large amount of impurities such as proteins, salts, surfactants, preservatives, etc. In addition, the sample itself Hydrophobicity and acid-base properties also greatly affect ionization efficiency, so traditional matrices (such as α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), sinapinic acid (SA)) MALDI-TOF MS is difficult to ana

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