5 results about "Collision-induced dissociation" patented technology

An interference ion removal device used in a mass spectrometry system or the like comprises a first ion guide and a second ion guide, a DC potential barrier being formed between the first ion guide and the second ion guide. In the first ion guide, collision-induced dissociation (CID) and kinetic energy discrimination (KED) are performed, and in the second ion guide, the velocity distribution of ions is caused to converge.

To provide a technology for improving the accuracy of compound identification in liquid chromatograph mass spectrometers, including single quadrupole mass spectrometers. [Solution] In the analytical apparatus 100, the sample contains a target compound produced by the derivatization of a short-chain fatty acid or organic acid, which is the target compound in the analyte. The central control unit 5 causes the mass spectrometry unit 2 to measure the m / z intensity of the fragments while performing insource collision-induced dissociation on the sample during a given holding time in the liquid chromatograph, and also to measure the m / z intensity of the fragments on the sample during the same given holding time without performing insource collision-induced dissociation.

The present application belongs to the technical field of separation and analysis chemistry, and particularly relates to a mass spectrometric qualitative analysis method for iridoid glycoside isomers. The steps of the method are as follows: the sample is first extracted and separated by liquid chromatography, the separated sample is subjected to secondary mass spectrometric analysis under negative ion and positive ion modes under gradient collision energy, collision-induced dissociation is performed on the target parent ion, and secondary mass spectrometric spectra under various collision energies are collected; characteristic ions are screened, and isomers are identified by comparing the relative abundance differences of the characteristic ions between different isomers. The present application compares and analyzes the MS / MS cracking rules of iridoid glycoside isomers by LC-QTOF-MS / MS system, and the differences in cracking characteristics are determined by the relative intensity differences of multiple fragment ions under positive and negative modes under gradient collision, thereby improving the accuracy of isomer identification.

A mass spectrometer including: a reaction chamber into which a precursor ion derived from a sample molecule is introduced; a collision gas supply part configured to supply collision gas to the reaction chamber; a radical supply part configured to supply hydrogen radicals, oxygen radicals, nitrogen radicals, or hydroxyl radicals to the reaction chamber; a dissociation operation control part configured to control operations of the collision gas supply part and the radical supply part to generate the product ions by collision-induced dissociation and radical attachment dissociation of the precursor ion inside the reaction chamber, an ion detection part configured to mass-separate and detect ions ejected from the reaction chamber, and a spectrum data generation part configured to generate spectrum data based on a detection result by the ion detection part.

The invention relates to the technical field of analytical chemistry mass spectrum imaging, in particular to a P-B reaction online detection system and method, and the system comprises a sample fixing unit, a multi-dimensional control platform, a fluid transmission unit, an auxiliary catalysis unit and a mass spectrum acquisition unit. According to the present invention, the P-B reaction and the mass spectrometry imaging technology are combined, the P-B reaction is adopted to generate the oxetane structure at the double bond through the ultraviolet light induced [2 + 2] cycloaddition reaction, and the characteristic fragment is generated after the collision induced dissociation so as to achieve the accurate positioning of the double bond position, and solve the problem of the lipid isomer spatial distribution analysis; meanwhile, accurate positioning and analysis of lipid carbon-carbon double bond positions in a complex biological sample are realized by utilizing high sensitivity and spatial resolution capability of a mass spectrum imaging technology; the method can be applied to the fields of biomedical research, metabonomics and drug development, and an innovative analysis method is provided for lipid molecular structure analysis and function research.