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2 results about "Collision-induced dissociation" patented technology
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Collision-induced dissociation (CID), also known as collisionally activated dissociation (CAD), is a mass spectrometry technique to induce fragmentation of selected ions in the gas phase. The selected ions (typically molecular ions or protonated molecules) are usually accelerated by applying an electrical potential to increase the ion kinetic energy and then allowed to collide with neutral molecules (often helium, nitrogen or argon). In the collision some of the kinetic energy is converted into internal energy which results in bond breakage and the fragmentation of the molecular ion into smaller fragments. These fragment ions can then be analyzed by tandem mass spectrometry.
Mass spectrometric qualitative analysis method of iridoid glycoside isomer
The present application belongs to the technical field of separation and analysis chemistry, and particularly relates to a mass spectrometric qualitative analysis method for iridoid glycoside isomers. The steps of the method are as follows: the sample is first extracted and separated by liquid chromatography, the separated sample is subjected to secondary mass spectrometric analysis under negative ion and positive ion modes under gradient collision energy, collision-induced dissociation is performed on the target parent ion, and secondary mass spectrometric spectra under various collision energies are collected; characteristic ions are screened, and isomers are identified by comparing the relative abundance differences of the characteristic ions between different isomers. The present application compares and analyzes the MS / MS cracking rules of iridoid glycoside isomers by LC-QTOF-MS / MS system, and the differences in cracking characteristics are determined by the relative intensity differences of multiple fragment ions under positive and negative modes under gradient collision, thereby improving the accuracy of isomer identification.
Owner:SHANDONG ANALYSIS AND TEST CENTER
Mass spectrometry method and mass spectrometer
A mass spectrometer including: a reaction chamber into which a precursor ion derived from a sample molecule is introduced; a collision gas supply part configured to supply collision gas to the reaction chamber; a radical supply part configured to supply hydrogen radicals, oxygen radicals, nitrogen radicals, or hydroxyl radicals to the reaction chamber; a dissociation operation control part configured to control operations of the collision gas supply part and the radical supply part to generate the product ions by collision-induced dissociation and radical attachment dissociation of the precursor ion inside the reaction chamber, an ion detection part configured to mass-separate and detect ions ejected from the reaction chamber, and a spectrum data generation part configured to generate spectrum data based on a detection result by the ion detection part.
Owner:SHIMADZU CORP