Method for evaluating traditional Chinese medicine preparation making technology based on metabonomics technology

A technology of traditional Chinese medicine preparations and metabolomics, applied in the direction of scientific instruments, instruments, measuring devices, etc., can solve the problems of unclear active ingredients, inability to support process improvement, and existence of unknown ingredients, and achieve objective and accurate evaluation standards, avoid Data processing and induction, effects of objective statistical significance

Inactive Publication Date: 2012-10-24
CHINA PHARM UNIV
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Problems solved by technology

However, traditional Chinese medicine preparations, especially compound preparations, have unclear active ingredients, many impurities, unknown ingredients, and complex effects (such as hawthorn: in the preparation, the main therapeutic effect is to strengthen the st...

Method used

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  • Method for evaluating traditional Chinese medicine preparation making technology based on metabonomics technology
  • Method for evaluating traditional Chinese medicine preparation making technology based on metabonomics technology
  • Method for evaluating traditional Chinese medicine preparation making technology based on metabonomics technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Evaluation of Ophiopogon japonicus extracts prepared with different extraction solvents

[0030] a: Preparation of Ophiopogon japonicus extracts with different processes

[0031] The commercially available Ophiopogon japonicus medicinal materials were crushed and sieved, and 1g of the crushed medicinal materials was extracted with 200ml of a mixed solvent of 35%, 55%, 75% and 90% ethanol and water respectively for reflux extraction twice. The two extracts were combined, and the combined extracts Rotary evaporation concentrator is concentrated at 55 degrees Celsius, and the concentrated extract is reconstituted with 100ml methanol and filtered with 5um filter membrane. In order to investigate the accuracy of the experiment, each extraction process was repeated 8 batches.

[0032] b: All samples to be tested are subjected to solid phase extraction

[0033] After each sample was filtered, solid phase extraction technology was used for impurity removal treatment. Solid phase extr...

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Abstract

The invention discloses a method for evaluating a traditional Chinese medicine preparation making technology based on a metabonomics technology, which comprises the following steps: 1, an orthogonal experiment design or a parallel contrast experiment design are used for acquiring traditional Chinese medicine preparation products with different preparation technologies; 2, the prepared products are subjected to sample pre-treatment; 3, a liquid phase series high resolution mass spectrometer is used for acquiring a chromatogram of the traditional Chinese medicine preparation product; 4, the belongingness components of the traditional Chinese medicine are found in the chromatogram according to mass loss strategy; 5, each component structure can be deducted according to fragment information obtained by determined accurate mass number and collision induced dissociation; 6, all belongingness components content in each product are subjected to quantitative analysis; and 7) the influence of the preparation technology on the preparation can be evaluated through multivariable data statistics analysis method. The method of the invention has the advantages of accuracy and sensitivity, and is not depend on standard compounds, and the change of belongingness components of the traditional Chinese medicine in the preparation product can be integrally traced.

Description

Technical field: [0001] The invention relates to the field of pharmaceutical production, in particular to the preparation process evaluation, quality evaluation and quality standard establishment of traditional Chinese medicine preparations or natural medicine preparations. Background technique: [0002] Traditional Chinese medicine preparations are developed on the basis of Chinese medicine prescriptions with good clinical application effects. They are widely used because of their multi-target and multi-effect effects, such as compound Danshen dripping pills, compound Danshen tablets, puerarin injection, step length Naoxintong, Mailuoning injection, etc. For a long time, traditional Chinese medicine preparations have played an important role in meeting clinical needs and promoting the development of Chinese medicine. However, there are also phenomena of unbalanced development, such as lack of basic research, backward preparation quality supervision system, unstable preparation q...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 王广基谢彤郝海平梁艳谢林康安戴琛郑啸刘林生龚平
Owner CHINA PHARM UNIV
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