The invention provides a preparation method of a duramater / spinal dural 
transplanting substitute which is obtained by repeated freezing and thawing of dural tissue, rolling and 
cracking of cells, crosslinking fixed protection, accellular 
antigen extraction, dense surface 
fibrosis modification, packaging and sterilization, and has the advantages of simple method, wide 
raw material sources, cheap raw materials, and low cost. The prepared dural substitute completely removes components of cells and other 
antigen components simultaneously when protecting dural tissue natural structure and properties, is good in 
biocompatibility, free of 
immune rejection, safe and reliable, good in mechanical performance, and easy in clinical operation, can meet the needs of 
defect repair, has the function of promoting tissue regeneration as a loose surface is beneficial to the 
tissue fluid adsorption, active factor enrichment, and growth of blood vessels and cells, and has the advantages of rapidness in fusion with a host, biodegradable absorption, and good repair effect. The animal test shows that the defect can be completely repaired without brain or spinal 
fluid leakage, or adhesion with 
brain tissue, and significant rejection is not found.