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Preparation method and device of duramater/spinal dural transplanting substitute

A substitute, a meningeal technology, applied in the field of tissue engineering medical biomaterials, can solve the problems of hyperplasia and encapsulation of connective tissue, the generation of an acidic microenvironment, and the failure to meet the ideal requirements, and achieves good biocompatibility, is conducive to adsorption, The effect of preventing cerebro/spinal fluid leakage

Active Publication Date: 2012-10-17
SHAANXI BIO REGENERATIVE MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both degradable and non-degradable polymer materials have the following defects: degradable polymer materials often produce an acidic microenvironment after degradation, causing severe inflammatory reactions and affecting the repair effect; Generally speaking, it is always a foreign body, which can easily lead to inflammation, connective tissue hyperplasia, infection, bleeding, etc.
Therefore, the existing decellularized antigen removal technology has not yet reached the ideal requirements

Method used

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  • Preparation method and device of duramater/spinal dural transplanting substitute
  • Preparation method and device of duramater/spinal dural transplanting substitute
  • Preparation method and device of duramater/spinal dural transplanting substitute

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Using bovine pericardium and peritoneum as raw materials, the dura / spinal transplantation substitute was prepared according to the following steps.

[0042] Step 1. Freezing and thawing: freeze the pretreated membrane tissue at -80°C for 3 hours, take it out and thaw it at room temperature; repeat the operation 2 times, and then wash it with PBS buffer or normal saline;

[0043] Step 2. Rolling: use a rolling device, control the rolling pressure to 9±1MPa, and the speed to 2±1rpm, repeatedly roll the frozen-thawed membrane tissue in normal saline for 5 times, and rinse it with ultrapure water;

[0044] Step 3. Cross-linking and fixing protection: Divide the rolled membrane tissue into about 12.5cm×12.5cm in size, place it at the opening of the container on one side of the split surface treatment device, and place the opening of the container on the other side opposite the membrane tissue. The high-quality sealing tape fixes the membrane tissue, the two containers are em...

Embodiment 2

[0050] Using porcine dura mater as raw material, the dura mater / spinal graft substitute was prepared according to the following steps.

[0051] Step 1. Freezing and thawing: the pretreated membrane tissue was frozen at -20°C for 12 hours, taken out and thawed at room temperature, and repeated 1 time, and then washed with PBS buffer and normal saline respectively;

[0052] Step 2. Rolling: use a rolling device, control the rolling pressure to 17±1MPa, and the speed to 7±1rpm, repeatedly roll the frozen-thawed membrane tissue in the air for 6 times, and rinse it with ultrapure water;

[0053] Step 3. Cross-linking and curing protection: Cut the rolled membrane tissue into a size of about 12.5cm×12.5cm, place it at the opening of the container on one side of the split-face treatment device, and place the opening of the container on the other side against the membrane tissue, The two containers are embedded, the sealing strip at the opening fixes the membrane tissue, and the bolts o...

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Abstract

The invention provides a preparation method of a duramater / spinal dural transplanting substitute which is obtained by repeated freezing and thawing of dural tissue, rolling and cracking of cells, crosslinking fixed protection, accellular antigen extraction, dense surface fibrosis modification, packaging and sterilization, and has the advantages of simple method, wide raw material sources, cheap raw materials, and low cost. The prepared dural substitute completely removes components of cells and other antigen components simultaneously when protecting dural tissue natural structure and properties, is good in biocompatibility, free of immune rejection, safe and reliable, good in mechanical performance, and easy in clinical operation, can meet the needs of defect repair, has the function of promoting tissue regeneration as a loose surface is beneficial to the tissue fluid adsorption, active factor enrichment, and growth of blood vessels and cells, and has the advantages of rapidness in fusion with a host, biodegradable absorption, and good repair effect. The animal test shows that the defect can be completely repaired without brain or spinal fluid leakage, or adhesion with brain tissue, and significant rejection is not found.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering medical biomaterials, and in particular relates to a preparation method and a device for a dural / spinal transplantation substitute. Background technique [0002] The dura mater and dura mater are important functional membrane tissues between the brain and skull, spine and spinal cord respectively, and have the same biological characteristics and functions. The dura mater is attached to the inner surface of the cranial cavity, loosely connected with the calvaria, and tightly combined with the skull base; the dura mater extends outward at the cranial nerve exit, migrates and combines with the capsule of the nerve, and extends downward at the periphery of the foramen magnum to form a hard ridge membrane. Open trauma, inflammation, tumor or other brain and spine diseases can cause damage to the dura / spinal mater. Damage to the dura / spinal mater often causes epilepsy, spinal fluid leakage,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
Inventor 黄文涛
Owner SHAANXI BIO REGENERATIVE MEDICINE CO LTD
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