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32results about How to "Coagulation does not occur" patented technology

Cryopreservation protection liquid for Wharton jelly tissues of human umbilical cord and preparation and application of cryopreservation protection liquid

The invention relates to cryopreservation protection liquid for Wharton jelly tissues of a human umbilical cord. The cryopreservation protection liquid comprises 5-10 percent of a permeable cryoprotectant, 1-5 percent of a non-permeable cryoprotectant, 10-20 percent of a knockout serum replacement and 70-80 percent of a serum-free basal culture medium. The invention also discloses a preparation method of the cryopreservation protection liquid. The preparation method specifically comprises the steps of (1) precooling the serum-free basal culture medium, the non-permeable cryoprotectant and the knockout serum replacement, mixing and uniformly shaking; (2) adding the permeable cryoprotectant; and (3) performing refrigeration under a temperature condition of 2-6 DEG C for more than 30min. The invention also discloses a cryopreservation method for the Wharton jelly tissues of the human umbilical cord by the cryopreservation protection liquid. The cryopreservation method comprises the following steps of (1) pretreating the umbilical cord; (2) peeling off the Wharton jelly tissues; and (3) cryopreserving the Wharton jelly tissues of the human umbilical cord. The cryopreservation protection liquid and the cryopreservation method which are used for treating the Wharton jelly tissues of the human umbilical cord have the advantages that injury to umbilical cord mesenchymal stem cells in a cryopreservation process can be alleviated, so that the activity and the clinical use safety of the umbilical cord mesenchymal stem cells are guaranteed.
Owner:重庆市红汇脐血干细胞中心有限公司

Liquid-phase CO2 methanation catalyst, preparation method and application of catalyst

The invention relates to a liquid-phase CO2 methanation catalyst, a preparation method and application of the catalyst. The liquid-phase CO2 methanation catalyst comprises amphipathic ionic liquid and metal active components dispersed in the amphipathic ionic liquid in the state of stable colloid, the grain sizes of the metal active components range from 0.5nm to 20nm, the spherical metal active components include a first metal active component and a second metal active component, the first metal active component includes nickel, the second metal active component includes one or mixture of more than one of lanthanum, cerium, molybdenum, ruthenium, ytterbium, rhodium, palladium, platinum, potassium and magnesium, and the molar ratio of the first metal active component to the second metal active component is 10:0.1-2. The metal active components in the liquid-phase CO2 methanation catalyst are small in grain size, narrow in distribution, wide in application prospect, easy to separate and recyclable, CO2 methanation of low-temperature liquid phase can be realized at the temperature of 100-200 DEG C, the liquid-phase CO2 methanation catalyst has excellent low-temperature catalytic activity, methane selectivity and heat stability, and the preparation method is simple and practicable, low in cost and easy in popularization.
Owner:WUHAN KAIDI ENG TECH RES INST CO LTD

Antibacterial and moisture preservation mask with mild touch sense and manufacturing method thereof

The invention relates to the technical field of cosmetics, in particular to an antibacterial and moisture preservation mask with the mild touch sense and a manufacturing method thereof. Glycerol contents are not added in a product; after the face is coated with the mask, the touch sense is mild but not sticky or greasy; under the effects of emulsifying and thickening ingredients such as acrylic ester/vinyl isodecanoate crosslinked polymers, dimethicone, carbomer and butanediol, after the still standing for 4 to 8 h after the coating of the product, the surface layer is in a half solidified state; the influence of the product on bedding, mattress and pillows under unconscious actions during deep sleeping can be reduced. In addition, through the use of nanometer silver with strong antibacterial and bactericidal effects, the complementary effect is achieved with allantoin, and D-panthenol antibacterial and bactericidal ingredients in the formula, so that the antibacterial and bactericidalcapability of the product is improved. The synergistic effect is achieved with moisture preservation ingredients of trimethylglycine trimethylglycocoll, phycite, sodium hyaluronate and the like in the product; the skin fine blood vessels, inflammation or acne are fast restored; meanwhile, watery and glossy states are restored.
Owner:GUANGDONG YALGET FINE CHEM

Calf artery blood sampling method

The invention discloses a calf artery blood sampling method. The calf artery blood sampling method includes the following steps that 1), a blood collecting barrel is washed with pure water 2-3 times,then the blood collecting barrel is soaked with 75% medical alcohol for 30-50 min, and the blood collecting barrel is put in a clean region for use after being dried; 2), a medical silicone tube and ablood receiving tool which are soaked with 2% sodium hydroxide are flushed with pure water 2-5 times, and then a blood drawing tool and a blood sampling tube with the two ends seal-bound by tin foilpaper are put in a high-pressure sterilization pot to be sterilized for use; 3), a calf is put on an operating table, conventional skin preparation is carried out on the neck artery of the calf, and then the skin is disinfected with a 5% bromogeramine solution; 4), after the skin is disinfected, the neck of the calf is cut up, an artery blood vessel is found, a small opening is cut up, the medicalsilicone tube is inserted into the small opening, the other end of the medical silicone tube is put in an inlet of the blood collecting barrel, and blood sampling is carried out. By means of the blood sampling method, rapid blood sampling can be achieved, the blood sampling amount is large, blood samples are not easily contaminated, and the method is suitable or large-scale use and popularization.
Owner:永城市汇丰生物科技有限公司

A cryopreservation solution for human umbilical cord Wharton's jelly tissue and its preparation and application

The invention relates to cryopreservation protection liquid for Wharton jelly tissues of a human umbilical cord. The cryopreservation protection liquid comprises 5-10 percent of a permeable cryoprotectant, 1-5 percent of a non-permeable cryoprotectant, 10-20 percent of a knockout serum replacement and 70-80 percent of a serum-free basal culture medium. The invention also discloses a preparation method of the cryopreservation protection liquid. The preparation method specifically comprises the steps of (1) precooling the serum-free basal culture medium, the non-permeable cryoprotectant and the knockout serum replacement, mixing and uniformly shaking; (2) adding the permeable cryoprotectant; and (3) performing refrigeration under a temperature condition of 2-6 DEG C for more than 30min. The invention also discloses a cryopreservation method for the Wharton jelly tissues of the human umbilical cord by the cryopreservation protection liquid. The cryopreservation method comprises the following steps of (1) pretreating the umbilical cord; (2) peeling off the Wharton jelly tissues; and (3) cryopreserving the Wharton jelly tissues of the human umbilical cord. The cryopreservation protection liquid and the cryopreservation method which are used for treating the Wharton jelly tissues of the human umbilical cord have the advantages that injury to umbilical cord mesenchymal stem cells in a cryopreservation process can be alleviated, so that the activity and the clinical use safety of the umbilical cord mesenchymal stem cells are guaranteed.
Owner:重庆市红汇脐血干细胞中心有限公司

Metal surface alloying method for promoting molten pool convection by non-uniform heat sources

The invention relates to a metal surface alloying method for promoting molten pool convection by adopting non-uniform heat sources. The method comprises the following steps that 1) a metal surface isheated by adopting a first heat source and molten to form a molten pool; 2) alloying gas enters the molten pool to carry out reaction to generate a high-melting-point compound, the compound has the tendency of solidifying to form a solidified layer, an area where the compound is located is heated by adopting a second heat source, it is guaranteed that the compound is not solidified, convection ofthe molten pool is promoted, an alloyed layer uniform in component and structure is formed, the first heat source and the second heat source are sequentially arranged in a heat source moving direction, and the power density of the second heat source is larger than that of the first heat source; and 3) the first heat source and the second heat source integrally move along the heat source moving direction, and the molten pool is solidified to form an alloyed layer. According to the method, laser gas alloying is carried out on the metal surface by adopting the non-uniform heat sources, and finally the alloyed layer with good structure and performance is obtained.
Owner:LANZHOU UNIVERSITY OF TECHNOLOGY

Anti-solidification large-temperature-difference LNG cold energy recovery heat exchanger

The invention discloses an anti-solidification large-temperature-difference LNG cold energy recovery heat exchanger. The recovery heat exchanger comprises a heat exchanger cavity, an intermediate medium is injected into the cavity, the upper portion of the cavity is an intermediate medium gas phase area, the lower portion of the cavity is an intermediate medium liquid phase area, an LNG coil pipe and an NG coil pipe are arranged in the gas phase area, and a heating medium coil pipe is arranged in the liquid phase area; a baffle is arranged on the upper portion of the heat exchanger and divides the upper portion of the heat exchanger into an LNG heating area and an NG heating area, the tops of the LNG heating area and the NG heating area are communicated, an LNG coil pipe is arranged in the LNG heating area, an NG coil pipe is arranged in the NG heating area, and the top of the LNG coil pipe is in communication with the bottom of the NG coil pipe; and a heat insulation baffle is arranged on the lower portion of the heat exchanger cavity and connected to a baffle, the lower portion of the heat exchanger is divided into a mixed preheating area and an evaporation area through the heat insulation baffle, the two areas are communicated at the bottom, a low-temperature heating medium coil is arranged in the mixed preheating area, and a high-temperature heating medium coil is arranged in the evaporation area. According to the recovery heat exchanger, solidification-free efficient recycling of LNG cold energy under the large temperature difference can be achieved, and a secondary refrigerant needed by ice making or air conditioning and the like can be directly prepared.
Owner:NORTH CHINA UNIV OF WATER RESOURCES & ELECTRIC POWER

A kind of preparation method of naphthol as

The invention relates to a preparation method of naphthol AS. The method comprises a condensation process, an alkali cooking process, a filtration process, a drying process and a tail gas recycling process, wherein the condensation process comprises the following steps: 1) adding chlorobenzene and an organic solvent into a suspension pot; 2) after stirring and mixing, adding 2-hydroxy-3-naphthoic acid and an oil soluble ionic dispersant, stirring and adding aniline into the suspension pot; 3) filling materials in the suspension pot into a condensation reaction kettle and dropwise adding mixed liquid of phosphorus trichloride; 4) after the completion of dropwise adding of the mixed liquid of the phosphorus trichloride, heating with steam to obtain condensation materials. The preparation method of the naphthol AS provided by the invention has the benefits that a produced naphthol AS product has good quality and high yield; as the oil soluble ionic dispersant and the organic solvent are added in the condensation process, the dispersion of particles in suspension liquid is more uniform, and the coagulation phenomena cannot occur, so that the condensation process reaction is complete, the time of the condensation process is shortened, and the economic benefit is improved.
Owner:JINING SUNSHINE CHEM
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