A cryopreservation solution for human umbilical cord Wharton's jelly tissue and its preparation and application
A technology of Wharton's jelly tissue and umbilical cord tissue, which is applied to the preservation, application, and artificial cell constructs of human or animal bodies, and can solve the problems of umbilical cord tissue temperature rise, long time and cost, and affecting freezing effects, etc., to achieve Accelerated inhibition of cell metabolism, good cryopreservation effect, and low toxic and side effects
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[0045] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0046] It should be noted that the reagents used in the present invention are all prior art and may be commercially available products.
1. Specific implementation
[0047] 1. Human umbilical cord Wharton's jelly
[0048] A cryopreservation solution for human umbilical cord Wharton's jelly tissue, the components and the volume fraction of each component are: 5-10% of permeable cryoprotectant, 1-5% of non-permeable cryoprotectant, serum replacement Product 10-20% and serum-free basal medium 70-80%.
[0049] During specific implementation, each component and the volume fraction of each component may be: 10% of the permeable cryoprotectant, 5% of the non-permeable cryoprotectant, 10% of the serum substitute and 75% of the serum-free basal medium.
[0050] More specifically, the components of human umbilical cord Wharton's jelly tissue...
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