Cryopreservation protection liquid for Wharton jelly tissues of human umbilical cord and preparation and application of cryopreservation protection liquid
A technology for Wharton's jelly tissue and human umbilical cord, applied in the biological field, can solve the problems of long time and cost, affecting the freezing effect, unfavorable quality control, etc., and achieve the effects of less damage, low toxic and side effects, and good freezing effect.
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[0045] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0046] It should be noted that the reagents used in the present invention are all prior art and may be commercially available products.
[0047] 1. Specific implementation
[0048] 1. Human umbilical cord Wharton's jelly
[0049] A cryopreservation solution for human umbilical cord Wharton's jelly tissue, its components and the volume fraction of each component are: 5-10% of permeable cryoprotectant, 1-5% of non-permeable cryoprotectant, serum replacement Product 10~20% and serum-free basal medium 70~80%.
[0050] During specific implementation, each component and the volume fraction of each component may be: 10% permeable cryoprotectant, 5% non-permeable cryoprotectant, 10% serum substitute and 75% serum-free basal medium.
[0051] More specifically, the components of human umbilical cord Wharton's jelly tissue and the volume ...
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