41results about How to "Lower synthesis cost" patented technology

This invention relates to the field of biopharmaceutical technology, providing a self-assembled peptide-rhein conjugate, its formulation, preparation method, and applications. The MMP-9-responsive self-assembled peptide-rhein conjugate of this invention, with the sequence Rhein-GFFPLGLAGACE, can respond to abnormally activated MMP-9 in atherosclerotic plaques, self-assembling in situ into a stable nanofiber structure at the lesion site. The rhein in this conjugate exhibits significant anti-inflammatory and antioxidant functions, but its water solubility and oral bioavailability are low. These are significantly improved after conjugation with the peptide, achieving targeted delivery and prolonging retention time. The introduction of the tripeptide ACE, which mimics the function of glutathione, further enhances the conjugate's ability to resist oxidative stress. Furthermore, this conjugate exhibits excellent anti-inflammatory and antioxidant effects, scavenging ROS, inhibiting inflammatory pathways, regulating macrophage phenotype, and inhibiting apoptosis, providing a new strategy for the treatment of atherosclerosis.

The invention discloses a method for preparing phenyl acetate with high selectivity. A phosphoric acid solution is added into a magnesium chloride solution, stirring and heating are performed to obtain a suspension, the suspension is dried to constant weight to obtain a white solid, the white solid is ground to obtain powder, and the powder is calcined to obtain the catalyst. Then mixing phenol and a catalyst, placing the mixture in an oil bath for reflux reaction, dropwise adding acetic anhydride, distilling after the reaction is finished, and collecting a fraction at 190-195 DEG C, namely phenyl acetate. According to the magnesium chloride loaded phosphoric acid type catalyst (MgCl2-H3PO4) prepared by the method disclosed by the invention, phenol and acetic anhydride react without a solvent, MgCl2-H3PO4 is a solid catalyst, can be recycled only by filtering and can be repeatedly used after being washed, and MgCl2-H3PO4 also improves the selectivity of phenyl acetate and reduces the production cost.

The invention discloses a xanthene nitroreductase-responsive fluorescent dye compound as well as a preparation method and application thereof, and belongs to the technical field of xanthene derivatives. The fluorescent dye compound can be used for quickly and specifically recognizing overexpressed nitroreductase in tumor cells; after the fluorescent probe acts with nitroreductase, the fluorescence intensity of the fluorescent probe at 718 nm is obviously enhanced, and the fluorescent probe has excellent targeting capability on mitochondria; meanwhile, under the excitation of illumination with the specific wavelength of 660 nm, an acting product of the fluorescent dye compound and nitroreductase can efficiently generate reactive oxygen species (ROS), and then the effect of specifically killing tumor cells is achieved. The xanthene nitroreductase response type fluorescent dye compound provided by the invention can achieve the goal of integration of diagnosis and treatment of solid tumors, and has important clinical application value.

The application discloses a kind of carolic acid and preparation method thereof, and specifically relates to the field of medical technology.The method comprises the following steps: stirring catalyst H2WO4 / C with hydrogen peroxide, then adding dichloro chrysanthemic acid ethyl ester; oil bath heating is carried out, reaction temperature is controlled, repeated reflux is carried out, and oxidation reaction is carried out; after the reaction, homogeneous transparent solution is filtered, extracted, and subjected to reduced pressure distillation to obtain carolic acid product.In the application, when catalyzing and oxidizing dichloro chrysanthemic acid ethyl ester, a high-activity H2WO4 / C heteropoly acid is used as a catalyst, the reaction scheme is optimized, the selectivity of the reaction is greatly improved, the number of cycles of the catalyst is increased, and after 20 cycles of the new catalyst, the catalyst activity does not decrease obviously.Furthermore, due to the great improvement of selectivity, the loss of product is reduced, and the yield is improved.

This invention belongs to the field of molecular biology and medical detection technology, and discloses a bladder cancer-specific nucleic acid aptamer, nucleic acid aptamer derivatives, and their applications. The nucleotide sequences of the nucleic acid aptamer are shown in SEQ ID NO: 1, 2, and 3. This invention screened three monoclonal nucleic acid aptamer DNA chains from bladder cancer tissue samples using the exponential enrichment systematic evolution (SELEX) technique and identified two target proteins that bind to these three nucleic acid aptamers. The nucleic acid aptamers specifically target the target proteins CAPRIN1 and FXR1 on the surface of bladder tumor cells. The nucleic acid aptamers obtained by this invention have advantages such as high affinity and specificity, no immunogenicity, simple chemical synthesis, stability, and ease of storage and labeling, providing a new molecular tool for the early diagnosis and targeted therapy of bladder cancer.

The invention provides a method for preparing chiral amine by catalyzing asymmetric hydrogenation of aromatic imine through a manganese / chiral ferrocene P, N, N ligand. The manganese precursor and ligand adopted by the method are easy to obtain, the reaction condition is mild, the operation is simple, the target chiral amine compound can be obtained with high yield and high stereoselectivity, and continuous large-scale preparation can be realized. The catalyst system is high in activity, the molar ratio of imine to the catalyst can reach 100000: 1, and the catalyst has a very good industrial application prospect.

The application discloses a method for constructing a multi-enzyme complex engineering strain, and has the characteristics that the method comprises the following steps: S1, constructing a light decarboxylase FAP and an oil-producing yeast fatty acid synthase fusion expression vector; S2, electrically shocking and transforming the constructed target vector into an agrobacterium competent AGL1, and introducing the target vector into a starting strain through an agrobacterium-mediated transformation method to obtain an engineering strain that is back-supplemented with a fatty acid defect; the engineering strain that is back-supplemented with the fatty acid defect is obtained, the FAP-FAS2 fusion expression is realized at the same time as the self-assembly of the intracellular FAS1, and a FAP-FAS synthase complex is obtained. The complex not only back-supplements the fatty acid defect function, so that the strain grows normally, but also can synthesize alkanes in the process of blue light irradiation fermentation culture, and realizes the intracellular assembly of protein macromolecules.

The invention discloses a novel oligopeptide PSP for angiogenesis and vascularized bone regeneration. The tripeptide innovatively screened by the invention is short in amino acid sequence, small in molecular weight, high in bioavailability, low in synthesis cost and easy to transport, modify and recombine. The tripeptide provided by the invention can significantly promote vascularized bone regeneration and improve skull defect repair. Good application prospects are realized.

The invention discloses a method for preparing a polyfluoroalkyl coumarin derivative, and belongs to the field of organic synthesis. According to the method, cheap and easily available polyfluorocarboxylic acid anhydride is used as a polyfluoroalkylating reagent, and in-situ activation of NHPI is combined to prepare the C3-site polyfluoroalkyl substituted coumarin derivative. Under the condition of visible light catalysis, polyfluoroalkyl carboxylic acid anhydride is used as a polyfluoroalkylating reagent, polyfluoroalkyl free radicals are generated through in-situ activation of NHPI, the free radicals and aromatic propiolate are subjected to a free radical cyclization rearrangement reaction, and finally preparation of the C3-site polyfluoroalkyl substituted coumarin derivative is achieved. The polyfluoroalkyl carboxylic acid anhydride used in the method is stable in property, low in price and easy to obtain, and the synthesis cost is reduced. The method is wide in applicable substrate range and applicable to various polyfluoroalkyl carboxylic acid anhydrides, and the tolerance of reaction functional groups is good.

This invention belongs to the field of organic light-emitting materials and semiconductor technology, and provides a multi-aryl aromatic heterocyclic compound, its preparation method, and an electroluminescent device. The structural formula of the multi-aryl aromatic heterocyclic compound is: [structure omitted]. Using this multi-aryl aromatic heterocyclic compound as the guest light-emitting material, the luminous efficiency of the electroluminescent device is effectively improved, its lifespan is extended, and the purity of the emitted color is significantly improved. The light emitted by the electroluminescent device is in the green to red light range.

The present application belongs to the technical field of zeolite molecular sieve, and particularly relates to an AFX type zeolite molecular sieve, a preparation method and application thereof. An initial gel obtained by mixing an aluminum source, a silicon source, an inorganic base (sodium carbonate and / or sodium hydroxide), seeds and water is subjected to a hydrothermal crystallization reaction, and a crystallization product obtained is subjected to solid-liquid separation, washing and solid drying in sequence to obtain the AFX type zeolite molecular sieve. The AFX type zeolite molecular sieve with no organic template agent and low silicon-aluminum ratio is synthesized by using a traditional hydrothermal synthesis method, using seeds and an inorganic cation double-oriented design, and has great potential application value in the future catalysis field of diesel vehicles and non-road mobile machinery and the industrial adsorption separation industry due to the properties of no organic template agent and low silicon-aluminum ratio, and the synthesis does not use an organic template agent, reduces industrial production cost, and also reduces environmental pollution, and has great economic advantages in industrial production.

This invention provides a method for preparing a high-breakdown-voltage plant-based synthetic ester insulating oil, belonging to the field of insulating oil preparation technology. The resulting plant-based synthetic ester insulating oil exhibits good insulation performance, low dielectric loss, suitable viscosity, and low synthesis cost. Under standard testing conditions, it has an acid value above 0.014 mgKOH / g and also possesses good viscosity, dielectric properties, and breakdown voltage, among other physicochemical characteristics. Furthermore, the materials are sourced from environmentally friendly and low-cost materials, making this method significant for the application of plant-based synthetic ester insulating oil in high-voltage, high-capacity transformers.

The application provides a denitration catalyst, a preparation method and application thereof, and the denitration catalyst comprises a layered MCM molecular sieve carrier and a composite oxide active component loaded on the surface of the layered MCM molecular sieve carrier, and the chemical formula of the composite oxide active component is CeCuO x The denitration catalyst has high surface acidity and redox capacity, the CeCuO x The alkali metal poisoning resistance of the catalyst is improved, so that the catalyst has high catalytic activity and nitrogen selectivity in the use temperature range of 250-400 DEG C after being poisoned by alkali metal.

The invention discloses a novel phase separation peptide for intracellular protein delivery and a synthesis method and application thereof. The structural formula of the novel phase separation peptide is shown in the specification. According to the novel phase separation peptide, coacervate microdroplets are formed through liquid-liquid separation (LLPS) self-assembly, meanwhile, protein can be rapidly collected, and after a coacervate enters cells by bypassing a classical endocytosis way, glutathione (GSH) in the cells triggers dissociation to release the protein.

The application discloses a CO-SCR catalyst, a preparation method and application thereof, and the CO-SCR catalyst comprises a cerium-based composite oxide carrier and a noble metal active component, and the noble metal active component is dispersed on the surface of the cerium-based composite oxide carrier by means of a surfactant. The CO-SCR catalyst utilizes the dispersion and loading of the noble metal assisted by the surfactant, so that the CO-SCR catalyst has higher metal dispersion and redox capacity. Meanwhile, the suitable pH value of the carrier surface is also beneficial to the adsorption of CO and NO, and the low-temperature catalytic activity and high-temperature stability of the catalyst are improved, and the CO-SCR catalyst has higher catalytic activity and nitrogen selectivity in the use temperature range of 250 DEG C to 400 DEG C.

The application discloses a diflunisal derivative, a preparation method and application thereof, and belongs to the technical field of agricultural fungicides. The preparation method comprises the following steps: mixing 2', 4'-difluoro-4-hydroxy-[1, 1'-biphenyl]-3-carboxylic acid, a condensing agent and an organic base in an ice bath and an inert organic solvent, and activating for 10-30 minutes; then adding an amine compound with a general formula of R-NH2, and stirring and reacting at room temperature for 20-28 hours; after the reaction is completed, extraction, washing, drying, concentration and column chromatography purification are performed, so that the target diflunisal derivative is obtained. The compound disclosed by the application has a novel structure, shows excellent inhibitory activity on cucumber botrytis cinerea, rice pellicularia sasakii, brassica rapa sclerotium, wheat fusarium graminearum and other plant pathogenic fungi, can be used as an active ingredient for preparing an agricultural fungicide or a fungicide composition, and provides a new lead structure for developing a new succinate dehydrogenase inhibitor fungicide.

This invention relates to the field of all-silica molecular sieve preparation technology, and discloses a method for preparing an MFI topological structure all-silica molecular sieve, the MFI topological structure all-silica molecular sieve and its application, comprising: (1) mixing molecular sieve mother liquor, organosilicate, organic template agent and KOH to obtain a colloidal mixture; (2) subjecting the colloidal mixture to a crystallization reaction; (3) performing solid-liquid separation on the crystallization product obtained in step (2) to obtain a wet molecular sieve and a crystallization mother liquor; drying and calcining the wet molecular sieve to obtain an MFI topological structure all-silica molecular sieve; (4) optionally concentrating the crystallization mother liquor, and then returning to step (1) to provide the molecular sieve mother liquor. This method can obtain highly crystallized, fine-particle all-silica-1 molecular sieves, reduce the raw material cost of molecular sieve synthesis, and improve the economics of the new gas-phase rearrangement process technology.

The invention discloses a nucleic acid aptamer of RUNX2 protein and application of the nucleic acid aptamer, and belongs to the technical field of biology, the nucleotide sequence of the nucleic acid aptamer is shown as SEQ ID NO.1, the nucleic acid aptamer has the advantages of being small in molecular weight, stable in chemical property, easy to store and mark and the like, the nucleic acid aptamer is obtained through screening based on the SELEX technology, the nucleic acid aptamer can be combined with the RUNX2 protein with high affinity and high specificity, and the nucleic acid aptamer can be used for detecting the RUNX2 protein. The affinity constant is as low as 8.88 nM, and the synthesis cost is low. The nucleic acid aptamer provided by the invention shows good application prospects in the aspects of disease detection, diagnosis, molecular imaging, targeted therapy and the like.

The application discloses use of cystatin A in preparation of a medicine for promoting skin wound healing, wherein the cystatin A is a small molecule secretory protein, the English name of which is Cystatin A, and the molecular weight of which is 12 kDa. The application proposes that the cystatin A is used for preparation of the medicine for promoting skin wound healing, in particular, a small molecule medicine taking the cystatin A as an active ingredient. The cystatin A applied locally on a wound can effectively promote skin wound healing, has high safety, is stable, can treat large-area skin wounds, has low synthesis cost, provides a new way for clinical treatment of skin wound healing, and has important clinical application value.

This invention belongs to the field of compound synthesis technology, and specifically relates to a method and application for the synthesis of ethyl 7-bromobenzo[D][1,3]dioxane-4-carboxylate. The synthesis method of this invention includes the following steps: 2,3-dihydroxybenzoic acid undergoes esterification to obtain ethyl 2,3-dihydroxybenzoate; ethyl 2,3-dihydroxybenzoate is mixed with a solvent, a catalyst and a brominating agent are added, and the reaction is carried out to obtain ethyl 4-bromo-2,3-dihydroxybenzoate; ethyl 4-bromo-2,3-dihydroxybenzoate is mixed with a solvent, a catalyst and a base are added, and the reaction is carried out; finally, diiodomethane is added to carry out a cyclization reaction to obtain the final product. This invention uses 2,3-dihydroxybenzoic acid as a raw material, and obtains the product through esterification, regioselective bromination, and cyclization reactions. Through a specific combination of brominating agent and catalyst, highly selective bromination at the 7-position of the benzene ring is achieved, inhibiting the formation of isomers at the 5- and 6-positions.

This invention discloses an electrochemical method for synthesizing aromatic cycloallylic compounds, belonging to the field of chemical synthesis technology. In this method, aryl diazonium salts and allyl sulfones are electrolyzed in an electrochemical reactor in the presence of a solvent and electrolyte by applying an electric current to obtain aromatic cycloallylic products. This invention uses electrochemical-driven processes to replace traditional transition metal catalysis, avoiding the problem of metal residue. It has advantages such as being environmentally friendly, low-cost, and having mild operating conditions, and has good prospects for industrial application.

A method for synthesizing defective niobium tungsten oxide anodes and its applications belong to the field of lithium-ion battery anode material preparation technology. The method, by precisely controlling the valence ratio of niobium ions in the raw materials and combining it with a specific annealing process, directly synthesizes niobium tungsten oxide materials with abundant and controllable cation vacancies (Nb vacancies or W vacancies) under an inert atmosphere. This method is simple, low-cost, and can effectively optimize the ion diffusion channels and structural stability of the material, reconstructing the bulk structure of niobium tungsten oxide, making it particularly suitable for preparing high-power, low-temperature resistant lithium-ion battery anode materials. This invention controls the valence ratio of niobium ions in the raw materials and combines it with a specific annealing process. 5+ / Nb 4+ The ratio allows for precise control over the type and concentration of Nb or W vacancies; the resulting material has abundant cation defects and open ion diffusion pathways, making it suitable for high-rate and low-temperature environments.

The application discloses a synthesis method of daclatasvir hydrochloride. The daclatasvir hydrochloride is obtained from biphenyl as a starting material through a Friedel-Crafts acylation reaction, a condensation cyclization reaction, an amide hydrolysis reaction, a condensation reaction, salt formation and the like. The synthesis method has the advantages of low synthesis cost, simple post-treatment method, high yield, industrial production, reduced solvent and catalyst use, reduced reaction by-products and pollution, conformity to the development trend of green chemistry, maximized reduction of the production cost of the overall process and high application value.

The application discloses a kind of gas phase reactors of gradient energy plasma and use method.This gas phase reactor includes microwave plasma generating device, liquid collection barrel, outlet pipeline, inlet pipeline, concentric atomizer, gas path joint, spherical reaction chamber, main support plate, cooling cup, peristaltic pump, microwave plasma generating device is installed on the top of main support plate, the central embedding of main support plate is equipped with spherical reaction chamber, concentric atomizer is inserted in the top of spherical reaction chamber, outlet pipeline in the top of concentric atomizer is connected with the liquid collection barrel below main support plate through peristaltic pump and inlet pipeline, and circulation flow path is formed.The microwave plasma of the application is used to participate in organic synthesis reaction with energy gradient, without adding additional catalyst, reduce chemical use and discharge, reduce synthesis cost, promote the reaction of reactant and gas in solution, increase reaction dimension, device is small, energy is adjustable, suitable for laboratory and desktop micro chemical industry condition application.

The invention relates to a method for preparing a battery material with assistance of gel. The method comprises the following steps: synthesizing PCLA-PEG-PCLA; pCLA-PEG-PCLA is dissolved in deionized water, sodium ferrocyanide is added, and stirring continues to form a first solution; dissolving PCLA-PEG-PCLA in deionized water, stirring at normal temperature, then adding ferrous sulfate, and continuously stirring to form a second solution; under the protective atmosphere environment, the first solution is dropwise added into the second solution through a peristaltic pump, after full reaction is completed, centrifugation, water washing, alcohol washing and drying are sequentially conducted, and then the battery material is obtained, the chemical formula is NaxFe [Fe (CN) 6] y.zH2O, 0 < x < = 2, 0 < y < = 1, and 0 < z < = 3; the battery material prepared by using the method can reduce lattice defects and crystal water, improve the sodium ion content, and can improve the rate capability and cycle performance of the battery when being applied to the sodium ion battery.

The application discloses a synthesis method of xylulose-5-phosphate. The method takes a commercially available compound 1 as a starting material, and obtains the target product xylulose-5-phosphate through seven independent step-by-step reactions. The intermediates are compound 2 to compound 7 in turn. The synthesis method provided by the application is a step-by-step reaction, each reaction is an independent unit, the process parameters are accurately controllable and suitable for industrial operation errors, the side reactions are few, and the product selectivity is high. The method innovatively uses tetrabenzyl pyrophosphate as a phosphorylation reagent, replaces expensive reagents, and reduces the synthesis cost. The reaction condition of the method is mild, the operation is convenient, the crude compound 2 and the crude compound 4 can be directly used in the next step reaction, the post-treatment process is simple, and the route is simple and easy to be industrialized. The synthesis method of xylulose-5-phosphate has stable yield and accurate product structure, is suitable for large-scale industrialized preparation of xylulose-5-phosphate, and can effectively meet the market demand in the field of biological medicines.

The present application relates to a method for synthesizing a long-chain double-stranded nucleic acid from a short-chain single-stranded nucleic acid, said method being characterized by the presence of an intermediate, said intermediate being a long-chain double-stranded nucleic acid with a breach. In addition, the invention also relates to application of the method in synthesis of long-chain double-chain nucleic acid.

The application discloses an amphiphilic polyetherized alpha-keto(hetero)aryl thioester compound for LED photopolymerization, a preparation method thereof and application thereof. The amphiphilic polyetherized alpha-keto(hetero)aryl thioester compound can be used as an initiator for LED photopolymerization, has good absorption performance in a visible light region above 400 nm, especially shows good initiation performance under a 405 nm LED light source, can effectively initiate photopolymerization of water-based or oil-based photopolymerizable monomers, especially water-based or oil-based (methyl) acrylate monomers, avoids use of a noble metal catalyst, has a simple synthesis route and low synthesis cost, and thus has extremely high practical value.

The invention discloses deuterated tetra-substituted silanol and a preparation method and application thereof.The structural formula of the deuterated tetra-substituted silanol is shown in (I), and the preparation method of the deuterated tetra-substituted silanol comprises the step that in the presence of alkali, heavy water and a solvent, a functionalized benzo-silicon hetero-six-membered ring compound is obtained through intramolecular selective Si-C bond breakage and deuterated reaction; the compound provided by the invention can be applied to preparation of a deuterated silanol-containing drug, and can be used as an intermediate for drug synthesis. In the product range, richer and more complex functional groups can be introduced, the product range is greatly widened, a foundation is laid for subsequent application transformation, and the method can be widely applied to the fields of material chemistry and medicinal chemistry.