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This invention discloses a ketoisomerase mutant and its application in the preparation of D-chiral inositol, belonging to the field of genetic engineering technology. The ketoisomerase mutant is obtained by mutating the amino acid sequence shown in SEQ ID NO.1, specifically by mutating amino acid position 62 and / or amino acid position 175 of the amino acid sequence shown in SEQ ID NO.1. After the above-mentioned mutation of the wild-type thermostable ketoisomerase, the resulting ketoisomerase mutant not only retains the advantages of the wild-type thermostable ketoisomerase—its heat resistance and susceptibility to temperature fluctuations—but also exhibits higher enzyme activity. When co-catalyzing the reaction of muscle inositol to D-chiral inositol with inositol dehydrogenase, it can further improve the conversion rate and yield of D-chiral inositol even at high substrate concentrations.

The application discloses a D-mangiferoline dehydrogenase MtOEPa mutant and application thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the D-mangiferoline dehydrogenase MtOEPa mutant is shown as SEQ ID NO. 4. The D-mangiferoline dehydrogenase MtOEPa mutant has stronger affinity to a substrate myo-inositol, higher catalytic efficiency and higher enzyme activity, and can significantly improve the conversion rate of a reaction and the yield of D-chiro-inositol when catalyzing the reaction of myo-inositol to generate D-chiro-inositol.

The invention discloses a D-formononeol dehydrogenase MtOEPa mutant and application thereof, and belongs to the technical field of gene engineering. The amino acid sequence of the D-formononol dehydrogenase MtOEPa mutant is as shown in SEQ ID NO. 4. The D-formononeol dehydrogenase MtOEPa mutant disclosed by the invention has stronger affinity to a substrate muscle inositol, higher catalytic efficiency and higher enzyme activity, and can significantly improve the conversion rate of the reaction and the yield of the D-chiral inositol when catalyzing the muscle inositol reaction to generate the D-chiral inositol.

The present disclosure provides a lighting device and a method and system for controlling the lighting device, wherein the lighting device control method is a method for controlling the lighting device by an effect application executed by at least one processor on a director terminal, comprising the steps of: generating a library setting a light emitting mode of a plurality of lighting devices; generating a scenario setting a light emitting mode of the plurality of lighting devices within a specified time; generating a performance preparation data including at least one of the library and the scenario; providing the generated performance preparation data to the plurality of lighting devices; transmitting a lighting device control signal to the plurality of lighting devices, the lighting device control signal commanding execution, interruption or termination of at least one of the library and the scenario within the provided performance preparation data; and implementing a comprehensive lighting control performance effect according to the transmitted lighting device control signal.

The application discloses a solder paste printing device suitable for small-batch products, which comprises a polyester film fixing module, a transmission module and an adjusting module; the polyester film fixing module comprises a polyester film with a printing pattern; the transmission module is used for fixing and position adjusting of a to-be-printed substrate; the to-be-printed substrate is arranged between the polyester film fixing module and the transmission module; and the adjusting module is used for height adjusting of the to-be-printed substrate. The solder paste printing of different thickness requirements can be realized by changing polyester films with different thicknesses, and the height and position of the substrate can be adjusted. The device can be suitable for small-batch and multi-variety products, and high-precision solder paste printing is realized.