The invention discloses a device for manufacturing an artificial blood vessel through combination of bio-printing and electro-spinning technologies. The device comprises a material release unit, a rotary receiving unit, a horizontal moving platform, a vertical moving platform, a high-voltage power supply and a control unit, wherein the rotary receiving unit is located below the material release unit and comprises a rotary motor and a receiving rod; the rotary motor can drive the receiving rod to conduct rotary operation. The device has the following benefits: during operation, when the material release unit uses a bio-printing nozzle, a first stepping motor controls an extrusion pump to extrude out a cell-containing hydrogel material in a storage tank, and the extrusion pump can realize setting and controlling of the material release speed and quantity by the control unit; the vertical moving platform controls relative displacement of the whole material release unit to the rotary receiving unit, and can conduct vertical up-and-down displacement (such as 0.1 mm, 0.5 mm, 5 mm and continuous displacement), the displacement can be adjusted according to the extrusion requirement, and the distance between the bio-printing nozzle and the rotary receiving unit is adjusted through the displacement; the bio-printing nozzle and electro-spinning nozzles can work simultaneously or alternately, which is adjusted by the control unit according to actual demands.