A kind of drug-loaded nanomaterial and preparation method thereof

A drug-loaded nanometer and drug technology, which is applied in the field of drug-loaded nanomaterials and their preparation, can solve problems such as immunotoxicity, and achieve the effects of low toxicity and side effects, high yield, and convenience for mass production.

Active Publication Date: 2020-03-20
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Finally, chronic use of PEG can confer immunotoxicity

Method used

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  • A kind of drug-loaded nanomaterial and preparation method thereof
  • A kind of drug-loaded nanomaterial and preparation method thereof
  • A kind of drug-loaded nanomaterial and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] This embodiment provides a kind of mercaptolated hydroxyethyl starch and its preparation method, the chemical structural formula of the mercaptolated hydroxyethyl starch is:

[0107]

[0108] Among them, R is H or CH 2 CH 2 Oh.

[0109] The method consists of the following three steps:

[0110] (1) Dissolve 1 g of hydroxyethyl starch with a molecular weight of 25000 Da and a hydroxyethyl substitution degree of 0.5 in 20 mL of deionized water and stir until completely dissolved; then add 0.8 g of completely dissolved sodium hydroxide solution and stir fully to obtain Reaction solution A; then add 1 g of chloroacetic acid to reaction solution A, and continue to stir and react at 70 degrees Celsius for 3 hours to obtain reaction solution B; pour reaction solution B into methanol and stir to obtain suspension C; Liquid C was centrifuged to obtain a white precipitate, which was washed several times with methanol; the white precipitate was redissolved in deionized water...

Embodiment 2

[0114] This example provides a polydopamine nanomaterial modified by mercaptolated hydroxyethyl starch and a preparation method thereof. The preparation method comprises: getting 40 mg of PDA and dispersing it in 10 mL of deionized water, stirring and ultrasonicating for 30 minutes, using sodium hydroxide (concentration: 0.1mol / L) to adjust the pH to 10 to obtain suspension A; take 200 mg of HES-SH and disperse it in 10 mL of water to obtain solution B; slowly add solution B to suspension A, and then continue to sonicate for 30 minutes. Stir and react at room temperature for 24 hours to obtain reaction solution C; ultrafilter the reaction solution C to remove unreacted HES-SH to obtain HES-PDA suspension D, the molecular weight cut-off of the ultrafiltration tube is 100kDa, and the ultrafiltration speed is 4000 rpm / min, the ultrafiltration time was 10min, and the ultrafiltration times were 4 times; the suspension D was freeze-dried to obtain a black solid as HES-PDA. The infr...

Embodiment 3

[0116] This embodiment provides a polydopamine drug-loaded nanoparticle (DOX@HES-PDA) stabilized by hydroxyethyl starch and a preparation method thereof, and its preparation includes the following steps:

[0117] (1) Take 40 mg of PDA and disperse it in 10 mL of deionized water, stir and sonicate for 30 minutes, use sodium hydroxide and hydrochloric acid (both concentrations are 0.1 mol / L) to adjust the pH to 10, and obtain the suspension A;

[0118] Take 200 mg of mercaptolated hydroxyethyl starch (HES-SH) and disperse it in 10 mL of water to obtain solution B; slowly add solution B to suspension A, then continue ultrasonication for 30 minutes, and then stir and react at room temperature for 24 hours to obtain a reaction solution C;

[0119] The reaction solution C is ultrafiltered to remove unreacted HES-SH to obtain polydopamine (HES-PDA) suspension D modified by mercaptolated hydroxyethyl starch, the molecular weight cut-off of the ultrafiltration tube is 100kDa, and the u...

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Abstract

The invention provides a drug-loaded nano material and a preparation method thereof, the drug-loaded nano material comprises drug particles with a mass ratio of (0.01-2):1 and a polydopamine modifiedwith a thiolated hydroxyethyl starch, the periphery of the polydopamine is modified with the thiolated hydroxyethyl starch, the peripheral regions of polydopamine nanoparticles are uniformly coated with the drug particles, and the encapsulation efficiency of the drug particles in the periphery regions of the polydopamine is 30% to 100%; the preparation method comprises the following steps: 1, a mixture of the thiolated hydroxyethyl starch and the polydopamine is reacted under basic conditions to obtain the polydopamine modified with the thiolated hydroxyethyl starch; and 2, 1 part by weight ofthe polydopamine modified with the thiolated hydroxyethyl starch and less than 2 parts by weight of the drug particles are mixed for reaction to obtain the drug-loaded nano material. The preparationmethod of the drug-loaded nano material has simple operation and easy regulation of conditions, and the prepared drug-loaded nano material has strong loading ability and good biodegradability.

Description

technical field [0001] The present invention relates to drug-loaded nanomaterials, more specifically, to a drug-loaded nanometer material and a preparation method thereof. Background technique [0002] Polydopamine is a biological material that is polymerized by dopamine molecules under alkaline conditions. Since Messer Smith first reported the oxidative self-polymerization of polydopamine and the preparation method of polydopamine coating in 2007, many studies have been carried out. The work is dedicated to the study of the synthesis mechanism and related applications of polydopamine, forming a wave of research on polydopamine. [0003] The characteristics of polydopamine are mainly manifested in the following points: First, because polydopamine has abundant surface functional groups (-OH, -NH 2 ), showing special surface activity and strong adsorption capacity. Secondly, polydopamine can undergo inherent chemical reactions with nucleophiles, such as Michael addition reac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/14A61K47/34A61K47/36A61K31/704A61P35/00C08B31/12
CPCA61K9/146A61K31/704A61P35/00C08B31/125
Inventor 李子福杨祥良徐辉碧吴洪练
Owner HUAZHONG UNIV OF SCI & TECH
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