48results about How to "Broad medical application prospects" patented technology

The invention discloses a method for preparing antibacterial nanometer silver colloid, belonging to the technical field of nanometer biological materials. The method uses a chemical reduction method, comprising the following steps: dissolving water-soluble protective agent in silver nitrate water solution with the concentration of 0.001-0.1mol / L, wherein the mass ratio of protective agent to silver nitrate is 0.5-10.0:1; carrying out ice bath on sodium borohydride water solution with the concentration of 0.001-0.2mol / L for 10-30min; and dropwise adding the silver nitrate water solution containing protective agent in the sodium borohydride water solution with the speed of 30-60 drop / min, stirring by a magnetic stirrer, stirring for 20-40min after dropwise adding to prepare the nanometer silver colloid with the grain size of 5-80nm, wherein the mol ratio of the sodium borohydride to the silver nitrate is 1.0-30.0:1. The nanometer silver colloid prepared by the invention has the advantages of high concentration, high stability, high dispensability, and obviously-enhanced antibacterial property.

The invention relates to an external composition, and a preparation and an application thereof and belongs to the field of medicines. The external composition provided by the invention is a medicine composition which contains chlorhexidine or officinal salt thereof, or benzalkonium bromide or officinal salt thereof and a recombinant human epidermal growth factor or a recombinant cattle alkali fibroblast growth factor. According to the invention, the medicine composition is also further prepared into gel, liniment, ointment or paste, and the gel further comprises chitosan or officinal salt thereof and hyaluronic acid or officinal salt thereof. When being used for treating vagina inflammation, the external composition disclosed by the invention can improve a vagina microenvironment, promote growth of granulation tissue and repair vagina micro damage; and when being used for skin wound atraumatic restorative treatment, the external composition also can speed up restoration and regeneration of a wound tissue, promote healing and reduce scar formation, so that the external composition has a wide medical application prospect.

The invention discloses a preparation based on gold, silver, a gold-silver mixture and glutathione / chitosan, and an application of the preparation. According to the preparation and the application, the precious metal gold and silver nano medicinal preparation prepared by in-situ biosynthesis of targeted pathological cells / tissues has excellent biocompatibility, good permeability and high target performance, can achieve high-space-time resolution and accurate location of nidus parts such as tumors, and can facilitate apoptosis of tumor cells; the relevant precious metal preparation can target pathological loca such as the tumor cells / tissues in an organism, effectively facilitate the apoptosis of the tumor cells and ablation of the tumor tissues, and serve as the potential medicinal preparation for early treatment of the tumors clinically; and the relevant preparation and the application of the preparation are simple and easy, good in targeted treatment effect, low in toxicity, and visual in result, can conduct polymorphic, multi-mode, real-time, dynamic, and clinical monitoring and treatment, and have wide medical clinical application values and prospects.

The invention discloses a preparation method of platinum nanoclusters and application of the platinum nanoclusters in tumor imaging agents and apoptosis agents. The preparation method comprises the following specific steps of: mixing a platinum salt solution and ascorbic acid (VC) and reduced glutathione (GSH), and heating in a water bath, wherein the synthesis speed and the particle size of the platinum nanoclusters are controlled by adjusting the pH and temperature of a solution and a ratio of components. The prepared platinum nanoclusters have a small particle size, good permeability, and excellent biocompatibility and stable fluorescence characteristics, can be used for high spatial and temporal resolution imaging on tumors and other lesion sites, and can effectively promote tumor cell apoptosis and tumor tissue ablation; the method and the application are simple and easy to implement, the toxicity is low, the sensitivity is high, the multi-mode real-time dynamic monitoring can be carried out, and the medical clinical application value and prospect are broad.

The invention discloses detection reagents for fast and real-time dynamic monitoring and multimode imaging in the early stage of cardio-cerebrovascular related diseases. The reagents consist of transition metal ions and precious metal ions with good biocompatibility and nano-cluster and porphyrin derivatives thereof; non-intrusive real-time dynamic high-sensitivity fast tracing and monitoring of cardio-cerebrovascular related diseases are realized through accurate targeted fast labeling and imaging of related focus parts; the detection reagents can be applied to accurate detection, imaging and real-time dynamic monitoring of the focus parts of the diseases such as stroke, atherosclerosis, myocardial infarction, cerebral ischemia and cardiac ischemia as well as reperfusion injury thereof; the treatment can be guided by use of related focus images without causing any biological toxic or side effects; and the detection method is simple and easy to implement.

The invention discloses a method for synthesizing a rare earth metal compound nano cluster and application of the rare earth metal compound nano cluster in the aspects of target imaging of diseased regions such as tumor and contrast medium preparation. The rare earth metal compound nano cluster prepared by the method has a small particle size, good permeability, high fluorescent stability, high sensibility and strong targeting, so that the tumor can be accurately positioned; the rare earth metal compound nano cluster is directly synthesized by biological tumor cells and has good biocompatibility; and the method is convenient to operate and intuitive in result, synchronous analysis and detection in multiple states and multiple modes can be carried out, and the method has a wide medical application prospect.

The invention discloses a method for synchronously detecting polycyclic aromatic hydrocarbons and derivatives thereof in blood and application thereof, and the method comprises the following steps: selecting related polycyclic aromatic hydrocarbons and derivatives thereof in blood in the same blood sample as target detection substances, and carrying out synchronous extraction, detection and analysis; to be specific, the method comprises the following steps: S1, establishing a PAHs and SPAHs relationship analysis mathematical model, screening SPAHs having relatively strong correlation with PAHs, and taking the screened SPAHs and PAHs as target detection objects; S2, extracting a target detection object; S3, purifying and separating the sample; and S4, carrying out sample concentration and detection: performing analyzing on a machine to obtain content data of multiple components of detected target objects PAHs and SPAHs. According to the application of the method, detected content data of PAHs and SPAHs in a blood sample and the relation are applied to evaluation of exposure characteristics of two kinds of pollutants commonly existing in the environment in people or individuals, the detection effect and specificity are improved, and the application range of detection data is expanded.

The invention discloses a low-temperature plasma induced membrane-type artificial lung graft modification method. The modification method includes the following steps that poly 4-methyl-1-amylene membrane materials for a membrane-type artificial lung are placed into a cavity of a plasma processing instrument, working gas is any one or two of pure ammonia gas, pure oxygen, pure argon and pure carbon dioxide, the pressure of the working gas is kept to be 20 Pa, the materials are processed for 1-5 min at the discharge power of 30-300 W, the gas flow is 30 ml / min-120 ml / min, phosphoryl choline compound steam is led into the cavity to induce vapor phase grafting, the steam pressure is stabilized to be 20 Pa, and the materials are processed for 1-5 min at the discharge power of 30-300 W. According to the method, the artificial lung membrane materials are modified through the low-temperature plasma technology, the process is simple, consumed time is short, the efficiency is high, the energy consumption is low, the biocompatibility of a poly 4-methyl-1-amylene (PMP) membrane is obviously improved, and the transmission performance of O2 and CO2 is slightly reduced compared with that before modification, however, the method still has wide medical application prospects.

The invention relates to a novel endoscopy positioning device for digestive tract lesion and belongs to the field of medical instruments. On the basis of the space distribution characteristics of a magnetic field, the specific position of an in-vivo positioning mechanism is detected by means of magnetic force generated through mutual action of inside and outside magnetic fields of a human body; and the device comprises the in-vivo positioning mechanism, an in-vitro detecting mechanism and a display mechanism. A magnetic detecting rod which can enter an abdominal cavity from an opening formed in an abdominal wall by means of a laparoscope is adopted for detecting the suspicious lesion location of a digestive tract cavity; when a magnetic metal clip is detected, an electromagnetic switch of the detecting rod can be triggered, an indicator lamp is turned on, the lesion location is determined, generally, 2-3 magnetic metal clips can be placed on the opening side and the anus side of lesion for marking, the lesion range boundary is determined, and the problem of difficulty in positioning of lesion under the laparoscope is fundamentally solved.

The invention discloses a Chinese-Western compound preparation for treating senile dementia and a preparation method thereof. The Chinese-Western compound preparation is formed by compounding traditional Chinese medicine components and Western medicine components. The traditional Chinese medicine components are prepared from the following raw materials in parts by weight: 10-20 parts of rhizoma gastrodiae, 10-15 parts of radix salviae miltiorrhizae, 10-15 parts of folium ginkgo, 5-10 parts of cortex albiziae, 5-10 parts of fructus alpiniae oxyphyllae, 2-5 parts of dark plum, and 5-10 parts oflumbricus; the western medicines are composed of the following components: 1-1.5 parts of piracetam, 1-1.5 parts of donepezil hydrochloride and 0.5-1 part of vitamin B1. The method of combining Chinese and Western medicines can effectively improve the circulation functions of brain nerves, brain blood vessels and brain blood circulation, improves and treats insomnia, amnesia, hypomnesia and cognitive decline caused by senile dementia, has the advantages of short course of treatment, quick effect and low side effects, and has broad medical application prospects.

The invention discloses a modification method for processing membrane artificial lung surface by low-temperature plasma and grafting polyfluoride. According to the method, poly-4-methyl-1-amylene hollow fiber membranes are used as base membrane materials; a low-temperature plasma activation technology is used for introducing active sites on the membrane surface; one kind of polyfluorides from 1H, 1H, 2H-perfluor-1-hexene, perfluoro hexyl ethylene or 1H, 1H, 2H-perfluoro-1-decene is subjected to soaking grafting. The low-temperature plasma technology is used for introducing clinical application polyfluorides on the surface of the base membrane material; the excellent oxygen gas and carbon dioxide carrying capability is realized. The modified membrane artificial lung surface has the characteristics of promoting the mass transfer on the gas, so that the transportation performance on the oxygen gas and the carbon dioxide is greatly improved through being compared with that before the modification. Meanwhile, the polyfluorides have low surface tension and super-hydrophobic performance, the biocompatibility of the modified membrane artificial lung is obviously improved. Therefore the modified membrane artificial lung has wide medical application prospects.

The invention discloses a low-temperature plasma induced membrane-type artificial lung graft modification method. The modification method includes the following steps that poly 4-methyl-1-amylene membrane materials for a membrane-type artificial lung are placed into a cavity of a plasma processing instrument, working gas is any one or two of pure ammonia gas, pure oxygen, pure argon and pure carbon dioxide, the pressure of the working gas is kept to be 20 Pa, the materials are processed for 1-5 min at the discharge power of 30-300 W, the gas flow is 30 ml / min-120 ml / min, phosphoryl choline compound steam is led into the cavity to induce vapor phase grafting, the steam pressure is stabilized to be 20 Pa, and the materials are processed for 1-5 min at the discharge power of 30-300 W. According to the method, the artificial lung membrane materials are modified through the low-temperature plasma technology, the process is simple, consumed time is short, the efficiency is high, the energy consumption is low, the biocompatibility of a poly 4-methyl-1-amylene (PMP) membrane is obviously improved, and the transmission performance of O2 and CO2 is slightly reduced compared with that before modification, however, the method still has wide medical application prospects.

The invention discloses a reagent for early rapid detection of Alzheimer's disease and an image preparation for multi-mode imaging of the early rapid detection. The reagent consists of transition metal ions and noble metal ions which have excellent biocompatibility, and nano-clusters and porphyrin derivatives of the transition metal ions and noble metal ions. An imaging detection reagent which can be used for accurately targeting and rapidly marking relative focus positions can be synthesized in fixed points by virtue of in-situ biological action, so that highly-sensitive and rapid tracing and monitoring with non-invasive, real-time and dynamic modes can be achieved; and moreover, the reagent cannot cause biologically toxic or side effects, and is simple, convenient and feasible in detection method. By adopting the medicines disclosed by the invention, the early, rapid and multi-mode (such as CT, ultrasound, fluorescence and MRI) imaging detection of the Alzheimer's disease can be achieved; and the medicines have wide medical application prospects.

the invention discloses a separating method of ox marrow mesenchyma stem cell, which comprises the following steps: adding ox marrow single-cell suspension liquid in the Percoll cell separating liquid with density at 1.082 to separate; obtaining foggy single-core cell at liquid intersecting layer; inoculating in the plastic container; removing non-adhesive wall cell to obtain the product with separating purifying efficiency over 98%.

The invention belongs to the field of medicines, and in particular relates to a use of low-dose IL-2 for preparing a medicament for preventing and treating osteoporosis. The invention discloses a newmedical use of low-dose IL-2. The therapeutic medicine takes the low-dose IL-2 as a main active component, in a mouse disease model, after low-dose human recombinant IL-2 is administered, all indexesfor evaluating bone loss are significantly improved, the immunotherapy method obtains better practical therapeutic effects, and increases a safe and effective new medicine for clinically preventing and treating various types of osteoporosis, and further increases the new use to the clinical use of IL-2.

The invention relates to a pharmaceutical composition for treating Alzheimer's disease, and a preparation method of the pharmaceutical composition. The pharmaceutical composition is prepared by compounding a traditional Chinese medicine component and a western medicine component, wherein the traditional Chinese medicine component is prepared from the following raw material medicines in parts by weight: 10-30 parts of epimedium herb, 5-20 parts of radix rehmanniae preparata, 5-20 parts of rhizoma dioscoreae, 8-16 parts of folium ginkgo, 8-16 parts of safflower, 10-15 parts of lumbricus and 10-15 parts of rhizoma picrorhizae; the western medicine component is prepared from the following raw material medicines: 2-4 parts of nimodipine and 2-6 parts of donepezil hydrochloride. The pharmaceutical composition for treating the Alzheimer's disease is prepared by adopting a method of combining traditional Chinese medicines and western medicines; the multiple Chinese herbal medicines and westernmedicines are selected and combined, thus realizing a synergistic effect and further enhancing the treatment effect on the Alzheimer's disease; the pharmaceutical composition has the functions of resisting aging and improving memory, can be used for effectively treating the Alzheimer's disease, and is obvious in curative effect and low in side effects, thus having a wide medical application prospect.

The invention discloses a preparation and an application for magnetic nanometer microspheres used for magnetic relaxation targeted determination of cancer cells. According to the invention, superparamagnetic Fe3O4@PEG-COOH nanoparticles are prepared by using ferric acetylacetonate and dicarboxyl polyethylene glycol as raw materials through a one-step synthesis method; and the magnetic nanometer microspheres Fe3O4@PEG-COOH are prepared by using the superparamagnetic Fe3O4@PEG-COOH nanoparticles, N-hydroxysulfosuccinimide and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride as raw materials through a carbodiimide coupling method. According to the invention, the antibody protein on the surface of a magnetic nanometer microsphere can be combined with a receptor on a cancer cell membrane, so specific recognition is realized. Through measurement of the magnetic relaxation time of the magnetic nanometer microspheres under a pulsed magnetic field through an atomic magnetometer, accurate detection of cancer cells is achieved; high detection speed, high detection precision and low detection limit are realized; a new idea and a new path are provided for early diagnosis of cancers;and wide medical application prospect is obtained.

The invention discloses application of a gene FAM176A of a liver tissue and / or protein FAM176A coded by the gene FAM176A in serving as a target for resisting a hepatitis b virus or application of the gene or the protein in preparation of an anti-hepatitis b virus drug. Membrane-spanning protein FAM176A coded by the gene FAM176A of the liver tissue can inhibit duplication of HBV. An in vitro cell experiment and an in vivo animal experiment show that the FAM176A can inhibit the duplication of the HBV, and the structure of the protein FAM176A is forecast; moreover, a membrane-spanning region of the protein FAM176A is a key functional region for inhibiting the duplication of the HBV, which provides a new target for further development of a new anti-HBV drug; the target has a very wide medical application prospect.

The invention discloses application of honey to preparation of a reagent for the long-term dynamic near-infrared targeting imaging of inflamed parts. The reagent is honey, propolis or royal jelly with excellent biocompatibility, or the reagent is the mixture of honey and propolis or royal jelly or the mixture of propolis and royal jelly. The reagent can be used for imaging and marking related inflamed parts specifically, accurately and rapidly under the in-situ biological positioning action and tracing and monitoring related inflamed parts dynamically, sensitively and rapidly for a long time in a non-invading manner. In addition, the reagent has no biological side and toxic effects and can be used for examining conveniently. The reagent can be used for realizing the real-time long-term dynamic near-infrared targeting imaging and related model imaging of the inflamed parts and has wide medical application prospect.

　Disclosed in the present invention are a gold / silver fluorescent nanomaterial synthesized rapidly in-situ on the basis of biomolecules such as nucleosides, and a preparation method and a use for said material. In the invention, biomolecules such as nucleosides are used as a template for the rapid in-situ production of a gold / silver nanoalloy. The nanomaterial has excellent biocompatibility and stable fluorescence properties, is able to achieve highly-sensitive and rapid testing of the bodily fluid of both healthy people and patients having illnesses such as cancer, and facilitates diagnosis in respect of relevant physiological indicators. The method of the present invention allows for the detection of nucleosides and other related bio-substances in the bodily fluids of patients such as cancer patients. The invention thereby facilitates rapid, highly-sensitive, low-cost, and directly-observable testing for physiological indicators of patients such as cancer patients. The method is easy to apply, low in toxicity, highly sensitive, and high in specificity. Real-time, dynamic clinical testing can be implemented in multiple forms and modes, giving the invention great value and a wide range of prospects for medical / clinical application.

The invention belongs to the technical field of biological medicines, and discloses application of a compound 28d in preparation of a medicine for increasing the expression quantity of gamma-globin, and application in preparation of a medicine for treating beta-thalassemia or sickle-cell anemia.

The invention discloses application of honey to preparation of a reagent for the long-term dynamic near-infrared targeting imaging of inflamed parts. The reagent is honey, propolis or royal jelly with excellent biocompatibility, or the reagent is the mixture of honey and propolis or royal jelly or the mixture of propolis and royal jelly. The reagent can be used for imaging and marking related inflamed parts specifically, accurately and rapidly under the in-situ biological positioning action and tracing and monitoring related inflamed parts dynamically, sensitively and rapidly for a long time in a non-invading manner. In addition, the reagent has no biological side and toxic effects and can be used for examining conveniently. The reagent can be used for realizing the real-time long-term dynamic near-infrared targeting imaging and related model imaging of the inflamed parts and has wide medical application prospect.

The invention discloses a method for synthesizing a rare earth metal compound nano cluster and application of the rare earth metal compound nano cluster in the aspects of target imaging of diseased regions such as tumor and contrast medium preparation. The rare earth metal compound nano cluster prepared by the method has a small particle size, good permeability, high fluorescent stability, high sensibility and strong targeting, so that the tumor can be accurately positioned; the rare earth metal compound nano cluster is directly synthesized by biological tumor cells and has good biocompatibility; and the method is convenient to operate and intuitive in result, synchronous analysis and detection in multiple states and multiple modes can be carried out, and the method has a wide medical application prospect.

The invention discloses a synchronous detection method and application of polycyclic aromatic hydrocarbons and their derivatives in blood. The detection substance is extracted, detected, and analyzed synchronously, which includes the following steps: S1: Establish a mathematical model for analyzing the relationship between PAHs and SPAHs, screen SPAHs that have a strong correlation with PAHs, and use the screened SPAHs and PAHs as target detection substances ; S2: Extraction of the target detection substance; S3: Purification and separation of the sample; S4: Concentration and detection of the sample: analysis on the computer to obtain the content data of various components of the detection target PAHs and SPAHs. The application of this method is to apply the detected PAHs and SPAHs content data and relationship in the blood samples to assess the exposure characteristics of the two types of pollutants that are ubiquitous in the environment in the population or individuals, which improves the detection effect and specificity And expand the application range of detection data.

The invention relates to an external composition, and a preparation and an application thereof and belongs to the field of medicines. The external composition provided by the invention is a medicine composition which contains chlorhexidine or officinal salt thereof, or benzalkonium bromide or officinal salt thereof and a recombinant human epidermal growth factor or a recombinant cattle alkali fibroblast growth factor. According to the invention, the medicine composition is also further prepared into gel, liniment, ointment or paste, and the gel further comprises chitosan or officinal salt thereof and hyaluronic acid or officinal salt thereof. When being used for treating vagina inflammation, the external composition disclosed by the invention can improve a vagina microenvironment, promote growth of granulation tissue and repair vagina micro damage; and when being used for skin wound atraumatic restorative treatment, the external composition also can speed up restoration and regeneration of a wound tissue, promote healing and reduce scar formation, so that the external composition has a wide medical application prospect.

The invention discloses a method and application for synchronous detection of volatile pollutant exposure and effect markers in urine. The method selects OH-PAHs, mVOCs and 8-OHdG that may be correlated in the same urine sample as targets Detected substances are extracted, detected and analyzed synchronously, which includes the following steps: sample pretreatment; sample enrichment and separation; OHdG is detected on the machine, and the corresponding analysis is used to obtain the content data of various target detection substances in urine, and the correlation between the three is analyzed. The invention applies the analysis of the relationship between the content data of the target substance to evaluate the human body exposure characteristics of two types of pollutants. The invention enables synchronous detection and analysis of various target detection substances through an optimization scheme, simplifies the detection steps, reduces the detection cost, and expands the application range of the health exposure risk assessment results.

The present invention relates to the application of a chickpea extract in the preparation of medicines for treating androgen-dependent diseases. The preparation of the chickpea extract is to freeze-dry, pulverize, extract and concentrate the chickpea after germination to obtain Four components of chickpea germinated hypocotyl A, germinated chickpea cotyledon B, germinated chickpea whole bean C, and chickpea ungerminated whole bean D extract were carried out through the 5α-reductase in vitro screening model Through the screening of biological activity, the better active component is postgerminated hypocotyl A of chickpea, which has broad medical application prospects in the field of drugs for treating androgen-dependent diseases.

The invention discloses a preparation method of platinum nanoclusters and application of the platinum nanoclusters in tumor imaging agents and apoptosis agents. The preparation method comprises the following specific steps of: mixing a platinum salt solution and ascorbic acid (VC) and reduced glutathione (GSH), and heating in a water bath, wherein the synthesis speed and the particle size of the platinum nanoclusters are controlled by adjusting the pH and temperature of a solution and a ratio of components. The prepared platinum nanoclusters have a small particle size, good permeability, and excellent biocompatibility and stable fluorescence characteristics, can be used for high spatial and temporal resolution imaging on tumors and other lesion sites, and can effectively promote tumor cell apoptosis and tumor tissue ablation; the method and the application are simple and easy to implement, the toxicity is low, the sensitivity is high, the multi-mode real-time dynamic monitoring can be carried out, and the medical clinical application value and prospect are broad.