The invention discloses a preparation based on gold, silver, a gold-silver mixture and glutathione/chitosan, and an application of the preparation. According to the preparation and the application, the precious metal gold and silver nano medicinal preparation prepared by in-situ biosynthesis of targeted pathological cells/tissues has excellent biocompatibility, good permeability and high target performance, can achieve high-space-time resolution and accurate location of nidus parts such as tumors, and can facilitate apoptosis of tumor cells; the relevant precious metal preparation can target pathological loca such as the tumor cells/tissues in an organism, effectively facilitate the apoptosis of the tumor cells and ablation of the tumor tissues, and serve as the potential medicinal preparation for early treatment of the tumors clinically; and the relevant preparation and the application of the preparation are simple and easy, good in targeted treatment effect, low in toxicity, and visual in result, can conduct polymorphic, multi-mode, real-time, dynamic, and clinical monitoring and treatment, and have wide medical clinical application values and prospects.