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Method for synthesizing rare earth metal compound nano cluster and application thereof

A rare earth metal, synthesis method technology, applied in pharmaceutical formulations, echo/ultrasonic imaging agents, preparations for in vivo tests, etc., can solve the problems of difficult deep tissue imaging, weak signal-to-noise ratio, etc., and achieve good targeted imaging Effect, strong targeting, broad medical application prospects

Active Publication Date: 2013-06-12
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nanoparticles have been used as luminescent probes for optical imaging, but there are many disadvantages: first, autofluorescence from organic components of tissues can lead to weak signal-to-noise ratio; second, it is difficult to achieve deep tissue imaging because of the weakened signal of inner tissues The emission wavelength of the probe should be adjusted to the condition of tissue transparency

Method used

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  • Method for synthesizing rare earth metal compound nano cluster and application thereof
  • Method for synthesizing rare earth metal compound nano cluster and application thereof
  • Method for synthesizing rare earth metal compound nano cluster and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for synthesizing rare earth metal compound nanoclusters includes the following steps:

[0027] 1) The mixed solution of europium nitrate and lanthanum chloride with different concentration ratios such as 1 / 17, 1 / 18, 1 / 19, 1 / 20, 1 / 21, 1 / 22, 1 / 23 and hepatocarcinoma cells are cultured in the cell 37℃ in the box, containing 5% CO 2 Incubate together for 10 hours to synthesize rare earth metal compound nanoclusters;

[0028] 2) Quantitative analysis of rare earth metal compound nanoclusters with fluorescence spectrometer;

[0029] Hepatocarcinoma cells (HepG2) were selected as the research object. In the experimental group, hepatoma cells (HepG2) in the logarithmic growth phase were seeded in a 6-well plate at a density of 1.6×105 cells / well. After 24 hours of culture, they were added to the sterilized and fresh The mixed solution of lanthanum chloride and europium nitrate diluted in sterile DMEM medium was incubated in a cell incubator for 10 hours to synthesize rare ea...

Embodiment 2

[0031] A method for synthesizing rare earth metal compound nanoclusters includes the following steps:

[0032] 1) Put a mixed solution of 1mg / ml lanthanum chloride and 0.01mg / ml europium nitrate with liver cancer cells in a cell incubator at 37°C, containing 5% CO 2 Incubate together for 10 hours to synthesize rare earth metal compound nanoclusters;

[0033] 2) Qualitative and quantitative analysis of rare earth metal compound nanoclusters with fluorescence spectrometer, confocal fluorescence microscope, etc.;

[0034] Hepatocarcinoma cells (HepG2) were selected as the research object. In the experimental group, hepatoma cells (HepG2) in the logarithmic growth phase were seeded in a 6-well plate at a density of 1.6×105 cells / well. After 24 hours of culture, they were added to the sterilized and fresh A mixture solution of lanthanum chloride and 1 mg / ml europium nitrate diluted in a sterile DMEM medium with a concentration of 0.01 mg / ml.

[0035] In the control group, hepatoma cells (H...

Embodiment 3

[0038] A method for synthesizing rare earth metal compound nanoclusters is characterized by comprising the following steps:

[0039] 1) Put a mixed solution of 1mg / ml lanthanum chloride and 0.01mg / ml europium nitrate with breast cancer cells in a cell incubator at 37°C, containing 5% CO 2 Incubate together for 24 hours to synthesize rare earth metal compound nanoclusters;

[0040] 2) Qualitative and quantitative analysis of rare earth metal compound nanoclusters with fluorescence spectrometer, confocal fluorescence microscope, etc.;

[0041] Construct a nude mouse subcutaneously inoculated breast cancer model, culture the breast cancer cells for 24 hours, add a sterilized and diluted fresh sterile DMEM medium with a mixed solution of lanthanum chloride and europium nitrate, and incubate in a cell incubator for 24 hours. Rare-earth metal compound nanoclusters, after ultrasonically dispersing a solution containing rare-earth metal compound nanoclusters, are injected into nude mice by a...

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Abstract

The invention discloses a method for synthesizing a rare earth metal compound nano cluster and application of the rare earth metal compound nano cluster in the aspects of target imaging of diseased regions such as tumor and contrast medium preparation. The rare earth metal compound nano cluster prepared by the method has a small particle size, good permeability, high fluorescent stability, high sensibility and strong targeting, so that the tumor can be accurately positioned; the rare earth metal compound nano cluster is directly synthesized by biological tumor cells and has good biocompatibility; and the method is convenient to operate and intuitive in result, synchronous analysis and detection in multiple states and multiple modes can be carried out, and the method has a wide medical application prospect.

Description

Technical field [0001] The invention relates to the field of synthesis and imaging of rare earth metal compound nanoclusters, in particular to a synthesis method and application of rare earth metal compound nanoclusters. Background technique [0002] With the development of modern science and technology, imaging technology has aroused widespread interest among scientists, has been applied to the research of in vivo imaging and has achieved remarkable results. Hayashi et al. carried out a series of experiments using small animal tumor models established by fluorescently labeled tumor cells and a real-time fluorescent imaging system. RobertMH may observe tumor metastasis through in vivo fluorescent protein labeling of tumor cells and high-resolution small animal fluorescence imaging system. However, this technique still has many limitations, especially the tissue autofluorescence generated when the body is illuminated and the weak tissue permeability under the irradiation of cut-o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/04A61K49/08A61K49/22
Inventor 王雪梅高生平李水红
Owner SOUTHEAST UNIV
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