1441 results about "Fluorescent imaging" patented technology

A federated distributed computational system enables secure oncological therapy optimization through multi-expert integration and advanced uncertainty quantification. The system implements a multi-expert integration framework that coordinates domain-specific knowledge through token-space communication for precision oncological treatment, while maintaining secure cross-institutional data exchange. The architecture coordinates multi-scale spatiotemporal synchronization across computational nodes, with each node containing local processing capabilities for fluorescence-guided imaging, uncertainty quantification, and expert knowledge integration. Through a distributed graph architecture, the system enables advanced fluorescence imaging with wavelength-specific targeting, multi-level uncertainty estimation combining epistemic and aleatoric approaches, and multi-scale tensor-based integration with adaptive dimensionality control. The system implements light cone search and planning for adaptive treatment strategy optimization, enabling medical institutions and research organizations to collaborate on complex oncological therapy projects while maintaining strict data privacy controls.

The invention relates to the technical field of medical image processing and tumor treatment, and provides a tumor image boundary identification method and system fused with a fluorescence boundary, and the method comprises the steps: collecting a tumor dynamic fluorescence image sequence and breathing waveform data through a fluorescence imaging device; performing multi-scale noise suppression processing on the image sequence to generate a preprocessed image without artifacts; extracting fluorescence intensity distribution characteristics through a multi-layer characteristic weighted fusion network and generating an intensity distribution matrix; constructing a motion compensation model according to the breathing waveform data, and correcting the boundary displacement of the intensity distribution matrix; fusing the corrected fluorescence features and the tumor tissue texture features to generate an initial tumor boundary contour; iterative topological optimization is carried out on the initial boundary, and boundary discontinuity is eliminated; and mapping the optimized boundary to a space coordinate system of the laser treatment equipment, generating a boundary mapping model and updating the boundary mapping model in real time. The precision and safety of laser treatment can be improved, and damage to normal tissue is reduced.

Nucleic acid hybridization buffer formulations and uses thereof are described that yield improvements in hybridization specificity, rate, and efficiency. The buffer formulation composition includes a target nucleic acid; at least one polar, aprotic, organic solvent, and a pH buffer system, wherein the target nucleic acid is attached to the surface via hybridization to a surface bound nucleic acid tethered to the surface, and wherein the hybridization of the target nucleic acid and surface bound nucleic acid has a high stringency and annealing rate.

A system for determining a bacterial load of a target is provided. The system includes an adaptor for configuring a mobile communication device for tissue imaging and a mobile communication device. The adaptor includes a housing configured to be removably coupled to a mobile communication device and, an excitation light source for fluorescent imaging. The excitation light source is configured to emit light in one of ultraviolet, visible, near-infrared, and infrared ranges.

The invention provides a fluorescence imaging method and device based on light source intensity adjustment and a storage medium, and the method comprises the steps: in a PDD fluorescence mode, when a blue light source is turned on, after blue light is reflected by an observation tissue, filtering a spectrum of a green fluorescence wave band generated by excitation of riboflavin through a notch filter; in the photodynamic white light image mode, red light, green light and blue light emitted by the red light source, the green light source and the blue light source are adjusted through the light compensation module according to preset light power, so that the light is reflected by observation tissue and then passes through the notch filter to be white light, and the light compensation module controls the light power output by the red light source and the light power output by the blue light source to be consistent. The green light output power is higher than the light power of the red light or blue light. By combining the notch filter and the light compensation module, the problem that in the PDD fluorescence imaging process, green fluorescence generated by excitation of riboflavin affects the contrast ratio of PDD fluorescence imaging is solved, and meanwhile color rendition of traditional white light imaging can be guaranteed.

The invention provides a fluorescence imaging method suitable for bladder photodynamic diagnosis, and the method comprises the following steps: in a PDD fluorescence mode, when a blue light source is turned on, blue light is reflected by observation tissues, and a spectrum of a green fluorescence wave band generated by excitation of riboflavin is filtered through a notch filter so as to reduce interference of green fluorescence on PDD fluorescence contrast; in the photodynamic white light image mode, when the red light source, the green light source and the blue light source are turned on at the same time, after light is reflected by observation tissue, green fluorescence wave band spectrums are absorbed through the notch filter, illumination light of the green fluorescence wave band is enhanced through the light compensation module, and the light passing through the notch filter is white light. By combining the notch filter and the light compensation module, the problem that in the PDD fluorescence imaging process, green fluorescence generated by excitation of riboflavin affects the contrast ratio of PDD fluorescence imaging is solved, and meanwhile color rendition of white light imaging can be guaranteed.

The invention provides a calcium imaging neural signal extraction method and product based on self-supervised pre-training, and relates to the technical field of artificial intelligence. Based on a pre-training data set containing a large amount of unlabeled two-photon calcium fluorescence imaging data, a pre-training decoding network and a corresponding pre-training task are self-supervised, and a pre-trained space-time coding network is trained by taking minimization of a pre-training loss function as a target; the method comprises the following steps of: firstly, establishing an encoder and decoder network, establishing fine-tuning data with accurate space-time marked neuron calcium signal positions through simulation, simultaneously performing fine-tuning on the encoder and decoder network by taking minimization of a fine-tuning loss function as a target, and checking the algorithm extraction precision based on various actual imaging data to obtain a calcium imaging signal extraction algorithm network with strong generalization performance. Therefore, a calcium signal extraction model which can be generalized in various imaging conditions and model biological brain regions can be obtained through training without manual labeling, a high-accuracy neural signal extraction result is obtained, the application difficulty that a deep learning model lacks data generalization ability is overcome, and the accuracy of a neural signal extraction result is improved. Calcium imaging neural activity analysis based on self-supervised pre-training and wide in application is achieved.

The invention discloses a multi-fluorescence imaging device capable of adjusting visual field area and optical tomography, which comprises a multi-wavelength light source, the multi-wavelength light source comprises a wavelength variable light source or a light source array, and the wavelength variable light source and the light source array are independent and can realize multi-wavelength output. The optical fibers are mounted on the multi-wavelength light source and transmit light according to different wavelengths, the optical fibers are circularly placed at certain intervals, and the diameter of the optical fibers is changed according to applied voltage and applied temperature to form an annular magnetic disk. The wavelength of the exciting light can be freely adjusted by utilizing a multi-wavelength light source or realizing the exciting light with variable wavelength, a plurality of contrast agents are excited to obtain a multi-wavelength fluorescence image, the characteristics of the plurality of contrast agents can be simultaneously observed, and the universal fluorescence imaging device is widely applied to multi-antigen analysis and treatment. The method can be used for measuring lesion light cross-sectional images and has the advantage of analyzing lesion cross-sectional structures in real time.

The invention discloses a fluorescent composite nano material as well as a preparation method and application thereof, and belongs to the technical field of fluorescent materials. The fluorescent composite nano-material provided by the invention comprises carbon quantum dots with carboxyl modified surfaces and fluorescent nano-particles with amino modified surfaces, wherein the mass ratio of the carbon quantum dots to the fluorescent nano-particles is (0.8-3): 1; the carbon quantum dots of which the surfaces are modified with carboxyl groups are connected with the fluorescent nanoparticles of which the surfaces are modified with amino groups through amido bonds formed by dehydration condensation of carboxyl groups and amino groups; the molecular formula of the fluorescent nanoparticles is Cs2NaYCl6: Yb < 3 + > / Eu < 3 + >, the molar concentration of doped Yb < 3 + > is 15-25%, and the molar concentration of doped Eu < 3 + > is 8-12%. The fluorescent composite nanomaterial provided by the invention has the advantages of uniform particle size and high fluorescence emission intensity, has good fluorescence stability, and can be applied to the fields of humidity detection, fluorescence imaging and the like.

The invention discloses nanoparticles for a contrast agent as well as a preparation method and application of the nanoparticles, and relates to the technical field of nano composite materials. According to the nanoparticle for the contrast agent, a shell layer comprises polydopamine and albumin which are connected through a covalent bond, a core structure comprises a complex formed by metal ions and polyphenol, and the metal ions in the core structure and the polydopamine in the shell layer also have a coordination effect. Due to the fact that all the components are tightly connected, the rotation time of the nano-particles is prolonged, the T1 relaxation rate of the nano-particles is greatly increased, meanwhile, due to introduction of polydopamine, release of metal ions in a human body is avoided, the biological safety of the nano-particles is improved, albumin in the nano-particles can further improve the biocompatibility of the particles, and the nano-particles can be used for preparing the nano-particles. Therefore, the nanoparticles provided by the invention also have wide application prospects in the fields of fluorescence imaging and targeted drug delivery.

The invention provides a fluorescence imaging method and device based on light source proportion adjustment and a storage medium, and the method comprises the steps: in a photodynamic diagnosis PDD fluorescence mode, when a blue light source is turned on, after blue light is reflected by tissues, filtering the spectrum of a green fluorescence wave band generated by excitation of riboflavin through a notch filter, the interference of green fluorescence on PDD fluorescence contrast is reduced; in the photodynamic white light image mode, red light, green light and blue light are controlled to be imaged in a stroboscopic mode through the light compensation module, and the proportion of the light power of the red light, the green light and the blue light is controlled, so that the red light, the green light and the blue light are reflected by observation tissue, penetrate through the notch filter and then are synthesized into white light. By combining the notch filter and the light compensation module, the problem that in the PDD fluorescence imaging process, green fluorescence generated by excitation of riboflavin affects the contrast ratio of PDD fluorescence imaging is solved, and meanwhile color rendition of traditional white light imaging can be guaranteed.

The invention provides a fluorescence imaging method and device based on light source pass filtering and a storage medium, and the method comprises the steps: in a PDD fluorescence mode, when a blue light source is turned on, after blue light is reflected by an observation tissue, filtering a green fluorescence wave band spectrum generated by excitation of riboflavin through a notch filter, the interference of green fluorescence on PDD fluorescence contrast is reduced; in a photodynamic white light image mode, when a white light source is turned on, white light firstly passes through a band-pass filter to increase the proportion of a green fluorescence wave band spectrum to a red and blue fluorescence wave band spectrum, then the green fluorescence wave band spectrum is filtered through a notch filter, the notch filter reduces the proportion of the green fluorescence wave band spectrum, and then the red and blue fluorescence wave band spectrum is filtered out. And the difference value of the proportion of the green fluorescence wave band spectrum increased by the band-pass optical filter is within a preset range. The problem that in the PDD fluorescence imaging process, green fluorescence generated by excitation of riboflavin influences the contrast ratio of PDD fluorescence imaging is solved, and meanwhile color rendition of traditional white light imaging can be guaranteed.

The invention discloses a full-automatic intelligent multifunctional high-order nonlinear fluorescence dynamics testing method and device, and relates to the field of nonlinear optics and nano photonics. An optical testing device composed of an exciting light generation module, an automatic power regulation module, an exciting light modulation module, a sample scanning module, a data acquisition and analysis module and an intelligent control module is established, and under the excitation of incident laser, based on the overall coordination of the intelligent control module, the optical testing device is established. Other modules are driven to complete the dynamic response test of a nonlinear fluorescence sample, and full-automatic intelligent, multifunctional, high-precision, simple, efficient, low-cost and universal fluorescence dynamic test analysis is realized. The problems that traditional manual operation wastes time and labor and the testing precision is low are solved. In addition, the multifunctional integration of the fluorescence response curve, the fluorescence rise time, the fluorescence lifetime and the fluorescence imaging effectively improves the test efficiency and the application scene, and greatly reduces the system cost.

The invention discloses a dual-mechanism antibacterial photosensitizer as well as a preparation method and application thereof, and relates to the technical field of biomedical photoelectric functional materials. The invention relates to a triphenylamine derivative, which can realize fluorescence imaging of bacterial or fungal infection by utilizing fluorescence characteristics, can generate active oxygen under illumination to play a photodynamic therapy role, is suitable for preparing photodynamic therapy preparations and developing antibacterial materials, and can also be applied to the fields of environment and public health and tumor treatment. The problems that an existing photosensitizer is insufficient in broad spectrum, different in fungus drug resistance mechanism, limited in single treatment effect and the like can be effectively solved, and the photosensitizer has wide application prospects.

The present disclosure relates generally to medical imaging, and more specifically to techniques for identifying at least one lymph node of a subject using fluorescence images. An exemplary method comprises obtaining a fluorescence image of a field of view including the at least one lymph node of the subject; obtaining a template image; comparing the template image with the fluorescence image to obtain one or more similarity values; and identifying at least one portion of the fluorescence image that corresponds to a location of the at least one lymph node based on the one or more similarity values.

The invention discloses a UNET supervised network slice HE virtual staining method and system for pathological analysis, and belongs to the technical field of artificial intelligence digital pathological analysis. Global scanning imaging is performed on a pathological slice to obtain an HE scanning image and a fluorescence confocal scanning image, and a UNET supervised network data set is established; a UNET supervised virtual dyeing network model is constructed; training and verifying the UNET supervised virtual dyeing network model by using the UNET supervised network data set; and carrying out HE virtual staining on the fluorescence imaging pathological section image by using the trained UNET supervised virtual staining network model. The method has the remarkable advantages in the aspects of dyeing precision, dyeing efficiency and robustness, and automatic and rapid virtual dyeing of different types of biological tissue sections can be achieved without accurate image registration.

The invention relates to a structural design, synthesis and application of a biomass carbon dot modified poly L-cysteine-S-S-carboxylic acid amphiphilic polypeptide. The invention provides a structural design, synthesis and application of poly (L-cysteine-S-S-carboxylic acid) amphiphilic polypeptide (P-L-cys) modified by biomass carbon dots (CDs). Comprising synthesis of P-L-cys which contains hydrophobic groups, provides hydrophilic and functionalized carboxyl and provides reduction response by disulfide bonds, synthesis of CDs and CDs modified P-L-cys (CDs (at) P-L-cys), and the macroscopic performance change of the polypeptide is explored from regulation factors of a polypeptide secondary structure and a self-assembly nanostructure assembly mechanism. The P-L-cys and the CDs (at) P-L-cys prepared by the invention have functionalization, fluorescence imaging and dual-response drug release and have particle sizes suitable for drug delivery and enrichment, and the CDs also provide a photo-thermal and photodynamic synergistic treatment effect and have good application prospects in the fields of drug delivery, cancer resistance and the like.

The invention discloses a cell culture and microscopic imaging integrated device which adopts an integrated design and comprises an imaging assembly, a fluorescence excitation assembly, an objective lens assembly, an automatic shading assembly, a two-dimensional displacement platform, a cell culture assembly, a bright field light source assembly and a shell, the imaging assembly, the fluorescence excitation assembly, the objective lens assembly, the automatic shading assembly and the two-dimensional displacement platform are integrated in the shell, the cell culture assembly is arranged on the two-dimensional displacement platform, and the bright field light source assembly is arranged above the shell. The device has the advantages that the bright field light source, the fluorescence excitation module, the imaging assembly and the cell culture assembly are integrated together, the device is complete in overall function, compact in structure, free of multiple sets of systems and convenient and fast to operate, the temperature and gas environment needed by cell culture are provided through the cell culture assembly, and the requirement for long-time real-time observation of living cell imaging is met; cell culture, multichannel fluorescence imaging and analysis can be completely realized through a single device, the operation process is simplified, and the working efficiency is improved.

Disclosed are systems and methods for continuously detecting, and optionally classifying, abnormal perfusion patterns in tissue by means of fluorescence imaging. A computer implemented method for detecting (and / or identifying) one or more areas having an abnormal perfusion pattern in tissue of a subject, for example during a medical procedure, includes: continuously acquiring fluorescence images of the tissue, wherein the fluorescence images are associated with a fluorescent output signal correlated with an input signal defined by a series of boluses of at least one fluorescent imaging agent, and wherein the series of boluses is administered with a predefined and / or controlled duration between subsequent boluses, analyzing the fluorescence images, identifying at least one tissue area with normal perfusion, defining a normal perfusion pattern (in an intensity domain and) in a time domain, and detecting, in the fluorescence images, possible tissue areas with abnormal (non-normal) perfusion pattern.

The invention relates to the technical field of plant living body nondestructive testing, and discloses a plant living body flavonol imaging detection method based on a multi-excitation light chlorophyll fluorescence imaging technology, which comprises the following steps: on the basis of the multi-excitation light chlorophyll fluorescence imaging technology, using a multi-excitation light chlorophyll fluorescence hyperspectral imaging function of a FluorTron multifunctional hyperspectral imaging system to carry out multi-excitation light chlorophyll fluorescence hyperspectral imaging on the basis of the multi-excitation light chlorophyll fluorescence imaging technology; collecting chlorophyll fluorescence spectrum images of the ginkgo leaves in a non-invasive and high-throughput manner; then, carrying out de-noising processing and fluorescence spectrum analysis on the spectral image by using FluorVision hyperspectral fluorescence imaging analysis software; and finally, analyzing Flav indexes of the flavonol model by using FluorVision hyperspectral fluorescence imaging analysis software, and making a graph. Compared with a traditional flavonol detection method, the method has the advantages of high throughput, non-contact, non-damage, digitization, visualization, in-vivo imaging detection and the like, in addition, a multi-excitation chlorophyll fluorescence spectrum imaging technology is adopted, the chlorophyll content, the anthocyanin content, the plant N element state and the like can be detected and analyzed at the same time, and the method is suitable for large-scale popularization and application. Therefore, the method has important application value and prospect in the fields of plant flavonol and anthocyanin high-throughput in-vivo detection, medicinal plant phenotype analysis, secondary metabolite imaging analysis, non-destructive rapid detection of traditional Chinese medicinal materials, in-vivo detection of functional components of fruits and vegetables, postharvest biology, remote sensing and the like.

The invention relates to an exterior mirror system and method for fluorescence imaging, and relates to the technical field of image processing, the system can detect fluorescence under a white light operation condition in a tumor excision process, and imaging in a dark or dim operating room is not needed; white light in the fluorescent light is separated, so that a clear fluorescent light image is presented; in addition, fluorescence images can be displayed in a 3D mode, and development is more visual. According to the invention, the technical problems of dark fluorescence image, poor definition and complicated operation in the developing process in the prior art are solved.

The invention discloses an intelligent endoscope system with a multi-mode imaging function, and relates to the technical field of medical equipment, and the intelligent endoscope system comprises an imaging module which comprises a white light imaging subunit, a fluorescence imaging subunit and an infrared thermal imaging subunit; the data preprocessing module comprises noise suppression, SIFT registration and normalization; the image fusion module is used for decomposing four layers in a Laplacian pyramid, and reconstructing after feature weighting; the intelligent analysis module is used for extracting multiple types of features, performing CNN-Transform model reasoning and performing historical case verification; the display control module is used for 4K display, AR labeling and touch operation; and the data storage module is used for AES-256 encryption, DICOM conversion and three-level permission. The diagnosis accuracy is improved through multi-modal fusion, lesions are analyzed and efficiently recognized, operation risks are reduced through operation navigation, safety is guaranteed through data double backup, the system is suitable for multi-cavity diagnosis and treatment, and clinical precise diagnosis and safe operation are assisted.

A system for determining a bacterial load of a target is provided. The system includes an adaptor for configuring a mobile communication device for tissue imaging and a mobile communication device. The adaptor includes a housing configured to be removably coupled to a mobile communication device and, an excitation light source for fluorescent imaging. The excitation light source is configured to emit light in one of ultraviolet, visible, near-infrared, and infrared ranges.

The invention provides a ratiometric fluorescent probe for adoptive NK cell marking and living body tracing and application of the ratiometric fluorescent probe. The ratiometric fluorescent probe comprises rare earth down-conversion nanoparticles of a core-shell structure, granzyme B response type polypeptide and a sensitizer, the surface of the rare earth down-conversion nano particle with the core-shell structure is coated with stearoyl phosphatidyl ethanolamine-polyethylene glycol. The ratiometric fluorescent probe disclosed by the invention has the characteristics of variable fluorescence signals under 808nm and 980nm laser excitation and self-carrying reference signals, and errors and instrument errors caused by different attenuation coefficients in tissues can be eliminated. The fluorescent probe can be used for adoptive NK marking, does not influence the biological characteristics of adoptive NK cells, and can be used for tracing the biological distribution and survival conditions of the adoptive NK cells in a host living body in a fluorescence imaging living body and evaluating the curative effect of adoptive NK cell treatment.

The invention provides an aza-BODIPY molecule with NIR-II fluorescence emission and I-type photodynamic activity and a preparation method thereof. The aza-BODIPY molecule has good light absorption in a near-infrared (NIR) region, the emission peak of the aza-BODIPY molecule is prolonged to a near-infrared second region (NIR-II), and the aza-BODIPY molecule shows an ultrahigh NIR-II fluorescence quantum yield of 13%. Meanwhile, the photosensitizer prepared by adopting aza-BODIPY molecules can effectively generate I-type active oxygen species, namely superoxide anion free radicals, under the excitation of 808nm laser. On the basis, high-resolution tumor fluorescence imaging can be realized by utilizing NIR-II fluorescence of the aza-fluorine boron fluorescent sensitizer, and the aza-fluorine boron fluorescent sensitizer is used for accurate and efficient NIR-II fluorescence imaging mediated I-type photodynamic therapy of deep tumor tissues.

A system for determining a bacterial load of a target is provided. The system includes an adaptor for configuring a mobile communication device for tissue imaging and a mobile communication device. The adaptor includes a housing configured to be removably coupled to a mobile communication device and, an excitation light source for fluorescent imaging. The excitation light source is configured to emit light in one of ultraviolet, visible, near-infrared, and infrared ranges.

The invention discloses a recurrence-resistant cell strain for colorectal cancer large segmentation radiotherapy and application, and relates to the technical field of cell biology. The colorectal cancer large segmentation radiotherapy recurrence-resistant cell strain is preserved in the China Center for Type Culture Collection on January 2, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: C202533. By constructing the colorectal cancer large segmentation radiotherapy recurrence-resistant cell strain, a new biological material is provided for studying the radiotherapy recurrence-resistant colorectal cancer. The cell strain is screened in a mouse body with a sound immune system, has better physiological correlation, and is beneficial to research on the influence of an immune microenvironment on tumor cell radiotherapy resistance and the remodeling effect of radiotherapy resistant cells on the immune microenvironment. The cell strain can also monitor tumor recurrence after radiotherapy in real time through fluorescence imaging.

The invention discloses an optical detection device and a sample analyzer, in the optical detection device, a bright / dark field illumination assembly is used for emitting an illumination light beam, and a fluorescence excitation assembly is used for emitting an excitation light beam; the bearing table is used for bearing a to-be-detected sample; the infinite objective lens and the barrel lens are used for imaging a to-be-detected sample in a bright field to form a bright field imaging light beam, imaging the to-be-detected sample in a dark field to form a dark field imaging light beam, and performing microscopic imaging on fluorescent particles in the to-be-detected sample to form a fluorescent imaging light beam; the imaging receiving assembly is used for receiving the bright field imaging light beam, the dark field imaging light beam and the fluorescence imaging light beam. The optical detection device is an infinite microscopic optical system, can perform bright / dark field detection on the to-be-detected sample, can perform fluorescence detection on the to-be-detected sample, is compact in structure, high in sharing rate of all optical elements, saves optical element materials, and is low in cost.

The invention relates to the technical field of medical image processing, in particular to a combined narrowband and fluorescence imaging method and system for bronchoscope focus detection. According to the method provided by the invention, based on deep learning, features with discrimination capability in a narrow-band imaging image and an auto-fluorescence imaging image are selected and extracted, fusion of different imaging technology advantages of focus detection is realized through decision fusion, and the speed and accuracy of focus identification are improved; meanwhile, the method provided by the invention can better adapt to different scenes and lesion features, has better generalization ability, improves the specificity and sensitivity of focus detection, and is beneficial to improving the diagnosis accuracy and efficiency.

The invention provides a multi-modal image fusion endoscope operation target positioning device and method, and the method comprises the steps: calibrating internal and external parameters of a binocular camera and a fluorescence camera, and obtaining a pose conversion matrix between the fluorescence camera and the binocular camera; using a U-Net network model to identify and track the position of the fluorescent mark point in the fluorescent image in real time, and obtaining the two-dimensional image coordinate of the fluorescent mark point; processing a left image and a right image of the binocular camera by using a region-based local stereo matching method in binocular stereo imaging to obtain a point cloud image of a target space; and obtaining three-dimensional pose information of the fluorescent mark point by combining the two-dimensional image coordinate of the fluorescent mark point and the point cloud image of the target space. The binocular stereoscopic vision and the fluorescence imaging technology are combined, the three-dimensional pose and depth information of the fluorescence mark point is obtained by registering the information between the fluorescence camera and the binocular camera, more accurate and real-time vision assistance is provided for an operation, and the safety and efficiency of the operation are improved.