357 results about "Fluorescent imaging" patented technology

The invention discloses a reactive compensation equipment fault automatic early warning system based on an intelligent algorithm, which belongs to the technical field of intelligent power grids and comprises a fault data acquisition module, a feature processing module, a fault diagnosis and evaluation module and a visualization module. Matlab software, Simulink software, a configurable point fast Fourier transform algorithm, a fluorescence imaging technology, a capacitive detection technology and a related intelligent algorithm are combined, reactive power compensation equipment fault analysis is carried out from three angles of a lightning arrester, a capacitor and a reactor, and the fault of the reactive power compensation equipment is analyzed in a chart form. And the visual output of the output result of the reactive power compensation equipment fault diagnosis model is realized. According to the invention, intelligent and precise diagnosis and visual early warning of reactive power compensation equipment faults are realized by comprehensively collecting multi-dimensional data of the lightning arrester, the capacitor, the reactor and overall compensation.

Fluorescence imaging systems and methods of are described herein that enable imaging of sequencing samples with a light source of a single color and a single image sensor. The optical systems herein provides higher signal efficiency, simpler optical filter design, and lower costs for DNA sequencing analysis and other imaging applications.

The invention discloses an anti-caries early warning method and system based on oral health detection, and belongs to the technical field of data processing. The method comprises the following steps: acquiring and processing target detection data to generate a clear enamel demineralization feature image and a buffer capability score; analyzing pixel-level fluorescence intensity distribution by adopting a convolutional neural network model, determining a quantized value of the enamel demineralization degree, and further determining a comprehensive dental caries risk level category; judging a risk change trend according to the historical target detection data sequence to obtain a dynamic evaluation update value; a data fusion algorithm is adopted to obtain a final decayed tooth risk assessment index, and risk early warning information is generated according to the early warning template; by integrating fluorescence imaging, an image enhancement algorithm, a convolutional neural network, spectral analysis, k-means clustering and time sequence analysis, a complete process from enamel demineralization feature extraction to comprehensive risk assessment is constructed, the accuracy and individuation level of decayed tooth risk assessment are remarkably improved, and a scientific basis is provided for early prevention and intervention.

The invention discloses a bubble self-stratification digital detection method and system for miRNA quantification of extracellular vesicles, and belongs to the technical field of biomedical engineering and molecular diagnosis. The technical problem to be solved is to provide a simple and sensitive tumor extracellular vesicle miRNA detection method capable of simultaneously detecting various miRNAs. According to the scheme, a to-be-detected sample and immunocapture bubbles coupled with an anti-EpCAM antibody are incubated to enrich tumor extracellular vesicles, a to-be-detected miRNA sample is obtained through in-situ ultrasonic lysis, then the to-be-detected miRNA sample, a fluorescence / DNA double-coding magnetic bead conjugate and the like are used for constructing an enzyme digestion system for enzyme digestion, multifunctional click glass microbubbles are added to capture unreacted magnetic beads, standing is conducted, self-stratification is conducted, bottom magnetic beads are collected, and the EpCAM antibody is obtained. After fluorescence imaging, an image is input into the AI automatic fluorescence counting and decoding module for counting to obtain a result. The method is used for detecting miR-21 and miR-155 in tumor extracellular vesicles, and is suitable for noninvasive early diagnosis of tumors.

The present disclosure relates to systems and methods for continuously detecting, and optionally classifying, abnormal perfusion patterns in tissue by means of fluorescence imaging. One embodiment relates to a computer implemented method for detecting (and / or identifying) one or more areas having an abnormal perfusion pattern in tissue of a subject, for example during a medical procedure, the method comprising the steps of: continuously acquiring fluorescence images of the tissue, wherein the fluorescence images are associated with a fluorescent output signal correlated with an input signal defined by a series of boluses of at least one fluorescent imaging agent, and wherein the series of boluses is administered with a predefined and / or controlled duration between subsequent boluses, analysing the fluorescence images, identifying at least one tissue area with normal perfusion, defining a normal perfusion pattern (in an intensity domain and) in a time domain, and detecting, in the fluorescence images, possible tissue areas with abnormal (non-normal) perfusion pattern.

The invention discloses an immunofluorescence imaging method for driving tyramine signal amplification based on chemiluminescence and application thereof, and relates to the technical field of biomedical imaging. The immunofluorescence imaging method comprises the following steps: carrying out first incubation on a to-be-detected sample and a peroxidase-labeled antibody; performing second incubation on the to-be-detected sample after the first incubation and a tyramine substrate containing a fluorophore under a dark condition; adding a chemiluminescent substrate solution into the to-be-detected sample after the second incubation, and carrying out image acquisition; wherein the antibody can be specifically bound with a target protein; the tyramine substrate can generate active free radicals under the catalysis of peroxidase, so that the active free radicals are covalently cross-linked with tyrosine residues; the chemiluminescent substrate solution comprises hydrogen peroxide and luminol. The immunofluorescence imaging method improves imaging signal and fluorescence stability, effectively reduces background interference and phototoxicity, is suitable for long-time stable fluorescence immunoimaging, and is especially suitable for high-sensitivity detection of low-expression protein markers.

The invention provides a wide-field imaging system and method based on a solid-state spin color center and a microwave characterization method.The wide-field imaging system comprises a probe, an optical detection unit, a microwave unit and a data processing unit, multiple times of microwave radiation opening and closing combined operation are carried out at the same frequency, fluorescence imaging is carried out respectively, and the detection result is obtained. And accumulating the fluorescence detection signals when the microwave radiation is started and accumulating the fluorescence detection signals when the microwave radiation is closed, and calculating the ratio of the two fluorescence detection signals. On one hand, the collection amount of fluorescence is increased through a method of accumulating exposure time, and the problem that camera exposure is easy to saturate is avoided; on the other hand, noise caused by factors such as light noise and vibration to detection can be effectively reduced through ratio processing, and the detection accuracy is improved; and the method can be well applied to characterization of a microwave field.

This invention relates to the field of biomedical image processing technology, and particularly to a cross-modal brain region image registration method based on differential homeomorphism. The method includes: registering the original T1-w image with an NMT-averaged standard template; registering the acquired cell architecture imaging and fluorescence imaging with the registered T1-w image; downsampling the fluorescence imaging and cell architecture imaging respectively; performing intensity correction; smoothing the cell architecture imaging / fluorescence imaging; re-downsampling the smoothed cell architecture imaging / fluorescence imaging; registering the T1-w image to the intensity-corrected cell architecture imaging / fluorescence imaging through affine transformation to obtain a deformation field; registering the T1-w D99 brain atlas to the cell architecture imaging / fluorescence imaging using the deformation field; and sampling the D99 brain atlas from the cell architecture imaging / fluorescence imaging to complete the registration. The advantages are: stronger robustness, higher accuracy, and no reliance on subsequent manual correction.

The invention discloses a preparation method and application of a mitochondrial targeting polypeptide modified carbon quantum dot probe, and relates to the technical field of nano biological materials and fluorescence imaging.The preparation method comprises the steps that citric acid and urea are dissolved in a dimethyl sulfoxide solution, mixed, heated, filtered, added with ethyl alcohol, centrifuged and freeze-dried, and carbon quantum dots are obtained; adding an MES buffer solution and an activating agent, reacting the polypeptide at room temperature, heating, reacting, centrifuging, dialyzing and freeze-drying to obtain polypeptide modified carbon quantum dots; the carbon quantum dots are synthesized from bottom to top, and the preparation method is simple. After polypeptide modification, the product has an obvious mitochondrial targeting effect, and the product is good in biocompatibility, so that the product can be used for imaging and dynamic tracking of living organelles. The probe developed by the invention shows obvious application potential in key cell events such as mitochondrial dynamic monitoring and the like.

The application belongs to the field of biomedical application, and more particularly relates to a cell fluorescence probe anchoring cell integrin, and a preparation method and application thereof. The cell fluorescence probe comprises a polypeptide complex, a fluorescent molecule and a cyclodextrin unit, the polypeptide complex comprises a cysteine-containing peptide, a peptide containing a bonding group and a targeting peptide which are connected in any order, the cyclodextrin unit contains a carbon-carbon double bond functional group, and is combined with the cysteine-containing peptide through a click reaction to form the polypeptide complex, the polypeptide complex is covalently connected to the fluorescent molecule through the bonding group, and the fluorescent molecule is an aggregation-induced emission compound. The double bond functional group of the modified cyclodextrin is bonded to the sulfhydryl group of the terminal cysteine of the polypeptide through a click reaction, and the sulfhydryl group of the terminal cysteine of the polypeptide is bonded to the double bond functional group of the modified cyclodextrin through a click reaction, so that the cyclodextrin is improved to protect the fluorescent molecule and prolong the fluorescent time effectiveness of the fluorescent imaging reagent.

Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the fields-of-view, improved imaging system duty cycle and a more compact system, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications at a lower cost.

A fluorescence imaging method and apparatus based on light source intensity adjustment, and a storage medium. The method comprises: in a PDD fluorescence mode, when a blue light source is turned on, reflecting blue light by observed tissue, and then filtering, by means of a notch filter, a spectrum of a green fluorescence band generated by excitation of riboflavin; and in a photo dynamic white light imaging mode, by means of a light compensation module and on the basis of preset optical power, adjusting each of red, green and blue light emitted by red, green and blue light sources, such that the light is reflected by the observed tissue, and then the light turns into white light after passing through the notch filter, wherein the light compensation module controls the output optical power of red light and the output optical power of blue light to be consistent, and controls the output power of green light to be higher than the optical power of red or blue light. By combining the notch filter and the light compensation module, the problem that green fluorescence generated by the excitation of riboflavin during PDD fluorescence imaging affects the contrast of PDD fluorescence imaging is solved, and color reproduction of conventional white light imaging can also be ensured.

The application belongs to the technical field of antitumor drugs, and particularly relates to an AuNRs@L / D-Ag2S QDs complex, a preparation method and application thereof. Sodium polystyrene sulfonate is electrostatically adsorbed on AuNRs to obtain 1-PSS-AuNRs; polyallylamine hydrochloride is electrostatically adsorbed on 1-PSS-AuNRs to obtain 2-PAH-AuNRs; polyallylamine hydrochloride is electrostatically adsorbed on 2-PAH-AuNRs to obtain a non-chiral nanomaterial; and the non-chiral nanomaterial is covalently crosslinked with L / D-Ag2S QDs to obtain the AuNRs@L / D-Ag2S QDs complex. The application combines the efficient photo-thermal conversion characteristics of AuNRs with the chiral-dependent targeting and potential fluorescence imaging ability of chiral Ag2S quantum dots by constructing a complex structure, so that a novel nanodiagnostic and therapeutic agent with efficient photo-thermal performance and chiral selectivity is obtained.

The invention relates to an estrogen receptor alpha and fibroblast activating protein dual-targeting near-infrared fluorescent probe as well as a preparation method and medical application thereof. Specifically, the probe can be used as a fluorescence probe for developing cells co-expressing the estrogen receptor alpha and the fibroblast activating protein, and can also be used as a near-infrared fluorescence imaging probe for diagnosing benign / malignant lesions such as nodules / tumors co-expressing the estrogen receptor alpha and the fibroblast activating protein. The fluorescent probe can also be applied to research on related biological events in cells for co-expressing the estrogen receptor alpha and the fibroblast activating protein, and particularly can be applied to fluorescent navigation in tumor resection operations for co-expressing the estrogen receptor alpha and the fibroblast activating protein; particularly, the composition can be applied to the synergistic treatment of an anti-estrogen therapy and a photodynamic therapy on a diseased region which co-expresses the estrogen receptor alpha and the fibroblast activating protein.

The invention provides a detection system of a composite silicon photomultiplier staggered array. The device is characterized by comprising a composite silicon photomultiplier array module (1), a dual-channel reading module (2), an ambient light monitoring module (3), a fusion control module (4), a multi-path bias power supply module (5), a high-sensitivity sub-array (6), a high-dynamic range sub-array (7), a metal bus line (8), a signal output channel 1 (high-sensitivity) (9), a signal output channel 2 (high-dynamic) (10) and a ceramic substrate (11). The system can be used for solving the technical problems that an existing single-type silicon photomultiplier detection system is low in photon detection efficiency under weak light and limited in dynamic range under strong light, and can be widely applied to the technical field of photoelectric detection with requirements for the dynamic range and sensitivity, such as automatic driving laser radar, biological fluorescence imaging and high-energy physical detection.

The invention discloses a targeted design and optimization method for an organic pollutant fluorescence sensing array and a signal acquisition device. The overall information of the array is obtained in a high-throughput manner in a fluorescence imaging mode; based on an inner filter effect sensing mechanism, the model selection of a sensing material and an optical device is carried out by taking the absorption spectrum characteristic of a target organic detection object as a starting point, and sensing optimization is carried out based on the controllability of weak interaction between the sensing material and the target detection object. The use process comprises two aspects of sample library construction and modeling and detection application. The method comprises the steps of sensing material selection, model selection of an excitation light source and a signal acquisition device, light path arrangement design and sensing performance optimization. The detection device disclosed by the invention has proper detection precision and portability, can replace a precise fluorescence spectrophotometer, and realizes low-cost, high-throughput and real-time field detection of a water body.

This invention discloses a bioactive glass and its preparation method, belonging to the field of bioactive glass technology. It aims to solve the problems of insufficient synergistic effect between antibacterial efficacy and bone-promoting activity in existing materials, as well as the inability to monitor in vivo degradation processes in real time. The bioactive glass comprises 44-48 parts silica; 22-27 parts calcium oxide; 22-27 parts sodium oxide; 5-7 parts phosphorus pentoxide; 2-4 parts europium zinc complex; 10-20 parts chitosan; 1-5 parts lidocaine; and 2-5 parts dilute acetic acid solution. The addition of the europium zinc complex promotes bone formation and enhances antibacterial activity, achieving a highly efficient synergy between anti-infection and tissue regeneration. Simultaneously, it provides optical tracking capabilities, allowing for non-destructive real-time monitoring of the material's degradation behavior and tissue integration in vivo via fluorescence imaging. This invention offers superior material performance and a simple preparation process, providing an innovative solution for the repair of infected bone defects.

This invention discloses a naphthimide-based fluorescent probe for detecting HClO and its preparation method. The fluorescent molecular probe uses naphthimide as the fluorophore and C=N as the specific reaction site for HClO. Due to the presence of the naphthimide fluorophore, the probe molecule exhibits two-photon properties, enabling deep tissue penetration, reducing background noise, and facilitating fluorescence imaging of biological systems. Furthermore, the introduced morpholine group allows for lysosomal targeting, enabling the monitoring of hypochlorous acid concentration levels in lysosomes, making it a valuable tool for early disease diagnosis. In addition, this type of probe molecule is simple to synthesize, operates under mild reaction conditions, exhibits stable optical properties, and has a high synthesis yield. It can detect a wide range of HClO, making it practically valuable in fields such as biochemistry and environmental science.

The embodiment of the invention provides a logging rock debris automatic detection method and system, and belongs to the technical field of logging rock debris detection. The method comprises the following steps: collecting a rock debris sample, and performing sample preparation treatment on the rock debris sample to obtain detected rock debris; respectively acquiring a white light dry illumination image and a fluorescence image of the detected rock debris, and performing image registration on the fluorescence image based on the white light dry illumination image; based on the white light dry illumination image, rock debris instance segmentation result recognition is executed, and a first recognition result is obtained; based on the registered fluorescence image, rock debris fluorescence imaging result recognition is executed, and a second recognition result is obtained; and performing exception removal processing on the first identification result and the second identification result, and outputting a detection result. According to the scheme, the problems that an existing logging rock debris detection scheme is low in detection efficiency and low in detection precision are solved.

The application discloses a method for imaging magnesium ions in living cells based on chemically modified deoxyribozyme, and belongs to the technical field of metal ion imaging. The method comprises the following steps: chemically modifying deoxyribozyme 10-23, screening and obtaining deoxyribozyme 10-23 (CaBn) which is modified with carboxyl (Ca) at the 8th position of the catalytic ring and benzene ring (Bn) at the 12th position; performing biochemical characterization and comparison; constructing a fluorescence sensor responding to magnesium ions; characterizing the performance; and applying the fluorescence sensor to imaging magnesium ions in living cells. Compared with the wild type, the application can catalyze the cleavage reaction under the condition of lower concentration of magnesium ions; therefore, the fluorescence sensor constructed from the deoxyribozyme can more effectively respond to lower concentration of magnesium ions in vitro, and the detection limit reaches 0.03 mM; in addition, the fluorescence sensor can perform fluorescence imaging on endogenous magnesium ions in living cells, and provides a new molecular tool for the field of molecular biology.

The invention discloses a macro lens for nucleic acid detection, and relates to the technical field of molecular detection. The macro lens for nucleic acid detection comprises a lens group, the lens group comprises a lens barrel, a first biconvex lens is fixedly arranged in the lens barrel, a first doublet lens is arranged on the right side of the first biconvex lens, a second doublet lens is arranged on the right side of the first doublet lens, and the second doublet lens is arranged on the right side of the second doublet lens. A third doublet lens is arranged on the right side of the second doublet lens, a fourth doublet lens is arranged on the right side of the third doublet lens, and a fifth doublet lens is arranged on the right side of the fourth doublet lens. According to the macro lens for nucleic acid detection, aberration is corrected through a specific bonding lens group so as to realize high-definition fluorescence imaging, the fluorescence collection sensitivity is improved through a large numerical aperture and long back focal length structure, an exciting light module is convenient to integrate by using a turning light path of a corner prism, and the overall layout is more compact.

The invention relates to the field of CAR-T cell concentration detection, in particular to a method for detecting CAR-T cells through combination of microfluidics and immunofluorescence. Through immunomagnetic bead enrichment and micro-fluidic chip partitioning, CAR-T cells are separated from a blood sample through immunomagnetic beads, then labeling is carried out or enhanced by adding a labeling antigen, anti-TCR-PE and DAPI, three-channel fluorescence labeling is achieved, quantitative analysis can be carried out on the number, the cell morphology and the function of the CAR-T cells through automatic immunofluorescence imaging, and the accuracy of CAR-T cell detection is improved. And the detection limit reaches 0.001%.

The invention provides a magnetic resonance compatible multispectral fluorescence mesoscopic imaging system, which comprises a fixing device located in a magnetic resonance cavity of magnetic resonance equipment and used for fixing an experimental object to be observed; the optical equipment comprises a light emitter, a light collector and a light conduction assembly, the light emitter is used for emitting light rays of at least two different wave bands, the light rays emitted by the light emitter are used for exciting fluorescence in an experiment object, and the light emitter and the light collector are both located outside the magnetic resonance cavity; and the computer is used for producing images. The system can realize synchronous acquisition of magnetic resonance imaging and multispectral mesoscopic fluorescence imaging when an experimental subject is in a waking state.

This disclosure relates to a surgical illumination device for generating a spot of light at a surgical site, used in conjunction with a fluorescence imaging device. The illumination device (1) includes: a support structure (2); a bottom surface (7) connectable to the support structure (2) to seal the lower surface of the device while allowing light to pass through; and a plurality of light sources (10) emitting white light and positioned between the support structure (2) and the bottom surface (7). The device also includes a plurality of NIR (near-infrared) filters (18), each NIR filter associated with a light source (10) and configured to substantially block transmission of wavelengths in the 680 nm to 900 nm band while minimizing variations in the color temperature of the spot of light.

The invention discloses a near-infrared ratio type fluorescent probe for rapidly and reversibly detecting histamine (HA), a preparation method of the fluorescent probe and application of the fluorescent probe in dynamic cell imaging, and belongs to the technical field of fluorescence imaging and biosensing. The preparation method comprises the following specific steps: synthesizing aza-BODIPY molecules (1a-1d, 2a-2d) with different halogenations, and meanwhile, connecting the aza-BODIPY molecules with hydroxyl groups at 3 and 5 sites with a hydrophilic PEG (Polyethylene Glycol) fragment and halogen to obtain an HA probe HAPCyto with good water solubility; in addition, the hydroxyl aza-BODIPY molecules are continuously subjected to substitution, halogenation and hydrolysis reaction to obtain carboxyl-containing aza-BODIPY molecules, then the aza-BODIPY molecules are subjected to amidation reaction by utilizing the site, and different organic fragments are respectively connected to obtain a cell membrane targeted HA probe HAPPM, a golgi apparatus targeted HA probe HAPGA and a HaloTag HA fluorescent probe HAPHaloTag. When HA exists in a solution, the developed probe can realize rapid reversible detection of the near-infrared ratio of histamine, also shows good reversible property in mast cells, and can realize dynamic monitoring of histamine in cells.

The invention relates to the field of medical image analysis, in particular to a drug distribution and metabolism dynamic analysis system of a multispectral microscopic image, which comprises a light source module, a drug distribution module, a drug distribution module, a drug metabolism dynamic analysis module and a drug metabolism dynamic analysis module, and is characterized in that the light source module emits a multiband light source comprising broadband white light and time sequence switching narrow-band monochromatic exciting light; the data acquisition module is used for acquiring a multi-channel spectral image signal and generating a multi-spectral image containing a spatial dimension and a spectral dimension; the pharmacokinetic analysis module constructs a geometric characterization framework of drug metabolism based on a differential geometry theory, and comprises a Riemannian manifold characterization unit for mapping multispectral data to a Riemannian manifold space, a curvature tensor analysis unit for calculating manifold curvature characteristics and a parallel transmission optimization unit for evaluating drug transmission efficiency; the application analysis module carries out drug target co-localization analysis, space-time metabolic map analysis and delivery system optimization based on the analysis result, the spectrum channel limitation of traditional fluorescence imaging is broken through, and the precision and dimension of drug metabolism analysis are remarkably improved.

The application discloses a fluorescence enhancement anchor agent based on a fluoroboron dipyrrin-tetrazine dye for inflation microscopy technology, and belongs to the technical field of biochemical analysis. The fluorophore of the fluorescence enhancement anchor agent is shown in formula (I), formula (II) or formula (III): the tetrazine group carried by the fluorescence enhancement anchor agent is removed from fluorescence quenching through a biological orthogonal reaction with a drug probe, and the drug probe is shown in formula (IV): the fluorescence enhancement anchor agent can covalently and non-covalently anchor small molecules in a hydrogel network based on a tetrazine biological orthogonal reaction, and realizes inflation microscopic fluorescence imaging of a double fluorescence enhancement mechanism. In addition, the drug probe selects a non-covalent drug, gefitinib, as a probe core, is modified with a BCN click handle, and successfully realizes in-situ fluorescence inflation microscopic imaging of an EGFR drug-target, thereby providing a new tool for research and clinical research on non-covalent drug target co-localization information of exosomes.

The application discloses a photosensitizer targeting beta amyloid, and a preparation method and application thereof. The photosensitizer is a photosensitizer with A-D-A configuration, and a structural formula thereof is shown as formula I. The photosensitizer synthesized in the application has a longer emission wavelength, and after being combined with beta amyloid, the fluorescence signal is obviously enhanced, and can effectively generate singlet oxygen, that is, the photosensitizer can target mark A beta protein, and is used for fluorescence imaging of the A beta protein; and after the photosensitizer synthesized in the application is combined with beta amyloid in the brain of an Alzheimer's disease (AD) model mouse, the cognitive ability of the AD mouse can be effectively improved, A beta-mediated neurotoxicity can be reduced, and clearance of A beta plaques can be promoted, that is, the photosensitizer can be used for early diagnosis and treatment of Alzheimer's disease.

This paper provides a method for detecting low concentrations of amphoteric compounds in a sample, comprising: (a) preparing an analytical sample mixture for capillary isoelectric focusing (CIEF) detection using the sample; and (b) detecting the analytical sample mixture using a fluorescence detector via a CIEF system; wherein the concentration of the low concentration amphoteric compound in the analytical sample mixture is less than 0.5 μg / μL. This method is more resistant to background noise interference and can sensitively detect and accurately quantify low concentrations of amphoteric compounds present in the sample.

The invention relates to a shock tube detonation test visualization device and a test method thereof, and the device comprises a shock tube which is sequentially divided into a premixing section, a detonation section, a development section and a measurement section along the axial direction, and the measurement section is provided with a plurality of optical visualization windows; the gas distribution ignition system is used for filling combustible premixed gas into the shock tube and generating a controllable ignition source at the detonation section; the synchronous signal acquisition system is used for acquiring a flame front position signal in the shock tube in real time; the schlieren system is used for acquiring a two-dimensional transient density gradient field of the shock wave and the flame front; the laser-induced fluorescence system is used for carrying out two-dimensional plane laser-induced fluorescence imaging on the flame reaction area; the high-speed photographing system is used for recording the overall propagation process of the flame in the shock tube; and the smoke grain acquisition unit is used for forming a smoke film on the inner wall of the measurement section to capture and retain the detonation cell structure. Compared with the prior art, the detonation wave three-dimensional structure and array surface change characteristics can be captured and observed at the same time through one detonation test.