Patents
Literature
70 results about "Structured illumination" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Self-Supervised Multimodal Structured Illumination Microscopic Reconstruction Method and System
A self-supervised multimodal structured illumination microscopic reconstruction method comprises: exciting a biological sample with structured illumination to obtain J raw fluorescence image sequences, wherein J is an integer greater than or equal to 1, each raw fluorescence image sequence comprises S fluorescence images, S being an integer greater than or equal to 2; generating a training set for each raw fluorescence image sequence among the J raw fluorescence image sequences; training a denoising neural network on the basis of the training set; and performing super-resolution reconstruction on the S fluorescence images in each of the J raw fluorescence image sequences using a standard structured illumination super-resolution reconstruction algorithm to form a super-resolution image, which is input into the denoising neural network to obtain a final super-resolution reconstructed image.
Owner:INSTITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
A method of monitoring the process of lysosomal acidification mediated by mitochondrial-lysosome interaction
The application belongs to the field of biomedicine, and discloses a method and a kit for monitoring a process of mitochondrion-lysosome interaction-mediated lysosome acidification. The method is characterized in that a cell lysosome content is first labeled with a LYSO-PZ probe with a specific structure, a pHrodo dye and a lysosome membrane marker sfGFP-LAMP1-mCherry are combined to monitor the pH value and membrane localization of the lysosome, respectively, then three-channel fluorescence images are acquired through a structured illumination microscope (SIM), and the fluorescence intensity ratio is quantitatively analyzed. In addition, the method further comprises a step of optogenetic regulation of mitochondrion-lysosome contact (MLC) formation, the fluorescence intensity change before and after the formation of MLC is compared through blue light induction of related protein binding, and the proton transport mediated by MLC is verified. The application first establishes a complete monitoring system, can accurately monitor lysosome acidification, and has important clinical application value and broad market prospect.
Owner:SOUTHERN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Mounting structure, lighting system and exposure equipment
PendingCN121596675APhotomechanical exposure apparatusMicrolithography exposure apparatusThermal deformationLighting system
The invention provides a mounting structure, a lighting system and exposure equipment, the mounting structure comprises a first mounting assembly fixed on a bottom plate, a second mounting assembly movably arranged on the bottom plate, and a flexible connecting piece connecting the first mounting assembly and the second mounting assembly, the first mounting assembly clamps one end of a dodging rod, and the second mounting assembly clamps the other end of the dodging rod. The second installation assembly clamps the other end of the light uniformizing rod, and the equivalent rigidity of the flexible connecting piece is smaller than the clamping rigidity of the first installation assembly and smaller than the clamping rigidity of the second installation assembly. Therefore, when the light homogenizing rod generates thermal deformation due to the change of the environment temperature caused by illumination in the illumination system, the light homogenizing rod can be ensured to generate thermal deformation only in the direction from the first mounting assembly to the second mounting assembly through the single-degree-of-freedom characteristic (namely, the flexible deformation in the axial direction of the light homogenizing rod) of the flexible connecting piece; the light-emitting surface on the light-homogenizing rod is prevented from deviating after thermal deformation, and the working index of the lighting system is ensured.
Owner:SHANGHAI MICRO ELECTRONICS EQUIP (GRP) CO LTD
Self-supervised multimodal structured illumination microscopic reconstruction method and system
A self-supervised multimodal structured illumination microscopic reconstruction method comprises: exciting a biological sample with structured illumination to obtain J raw fluorescence image sequences, wherein J is an integer greater than or equal to 1, each raw fluorescence image sequence comprises S fluorescence images, S being an integer greater than or equal to 2; generating a training set for each raw fluorescence image sequence among the J raw fluorescence image sequences; training a denoising neural network on the basis of the training set; and performing super-resolution reconstruction on the S fluorescence images in each of the J raw fluorescence image sequences using a standard structured illumination super-resolution reconstruction algorithm to form a super-resolution image, which is input into the denoising neural network to obtain a final super-resolution reconstructed image.
Owner:INSTITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
Realtime structured illumination super-resolution high-throughput imaging method
Systems and methods for image reconstruction are provided. The method may include: implementing a first thread to acquire image data of an object by imaging the object in at least one structured illumination direction using an imaging device, and storing the image data into a buffer; implementing a second thread to obtain one or more image sequences from the buffer; and / or implementing a third thread to extract at least one set of raw images from the one or more image sequences, and transmit the at least one set of raw images to a graphics processing unit (GPU) of the one or more processors, to facilitate the GPU to generate a target image based on the at least one set of raw images, each set of the at least one set of raw images corresponding to one of the at least one structured illumination direction.
Owner:GUANGZHOU COMPUTATIONAL SUPER RESOLUTION BIOTECH CO LTD
A three-dimensional shape measurement method and system based on continuous vertical scanning structured illumination
The application belongs to the technical field of three-dimensional topography measurement, and discloses a three-dimensional topography measurement method and system based on continuous vertical scanning structure illumination, which comprises the following steps: S1, collecting a structure light image of a sample to be measured at each scanning position; S2, estimating background light intensity by using adjacent structure light images in time domain, obtaining a uniform illumination image, and correcting the structure light image; S3, performing high-pass filtering on the uniform illumination image to obtain high-frequency in-focus information, and performing low-pass filtering on the corrected structure light image to obtain low-frequency in-focus information; fusing the two to obtain an optical tomographic image of the sample to be measured; and S4, obtaining the three-dimensional topography of the sample to be measured. The application adopts a continuous scanning structure light illumination measurement method, only one structure illumination phase shift cosine fringe pattern is collected at each scanning position, and high-precision tomographic extraction is performed, so that high-precision tomographic imaging in an uninterrupted continuous scanning mode can be realized, and the measurement speed and precision are greatly improved.
Owner:HUAZHONG UNIV OF SCI & TECH
Self-adaptive lighting headlamp mechanism of electric motor car
ActiveCN224131205Ureduce turn-on timeExtended service lifeOptical signalEnergy saving control techniquesAutomatic controlHeadlamp
The utility model discloses a self-adaptive lighting headlamp mechanism of an electric motor car, and relates to the technical field of lighting lamps. A self-adaptive lighting headlamp mechanism of an electric locomotive comprises an electric locomotive shell, a lamp mounting plate, a lighting lamp and a lighting adjusting structure, four fixing lugs are fixedly connected to the outer surface of the lamp mounting plate and fixedly mounted on the electric locomotive shell through bolts, and the lighting lamp is mounted on the lamp mounting plate. The lighting adjusting structure is located on the lamp mounting plate and comprises a fixing plate, a fixing box, two rotating shafts and a rotating plate, the light sensation sensor capable of being turned over is arranged on the headlamp, when the headlamp is turned on, the light sensation sensor automatically turns over to an effective sensing position, the headlamp is automatically controlled to be turned off in the daytime, and the lighting adjusting structure can be automatically turned off. Therefore, the unnecessary turn-on time of the headlamp is reduced, the working time of the bulb and related circuits is shortened, the aging speed is slowed down, and the service life of the headlamp is further prolonged.
Owner:JIYAN TECHNOLOGY (SHANGHAI) CO LTD
Engineered microscope slide for structured illumination
Microscope slide (100, 101, 102, 200, 300, 400) comprising: a transparent planar substrate (10, 30) for holding a sample (52), the substrate (10, 30) having at least one input port configured for receiving a light beam from a fiber (11, 21, 31) external to the substrate (10, 30); at least one optical circuit (12, 13, 14, 15, 16, 17, 18, 19, 20, 20', 22, 23, 23', 23'', 24, 24', 24'', 25, 25', 25'', 26, 26', 26'', 27, 27', 28, 29, 29', 29'', 31, 31', 32, 32', 33, 33', 41, 41', 42, 42', 43, 44, 45, 46, 47, 48, 49, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 170, 171, 172, 173, 174, 175) housed in the substrate (10, 30); said at least one optical circuit (12, 13, 14, 15, 16, 17, 18, 19, 20, 20', 22, 23, 23', 23'', 24, 24', 24'', 25, 25', 25'', 26, 26', 26'', 27, 27', 28, 29, 29', 29'', 31, 31', 32, 32', 33, 33', 41, 41', 42, 42', 43, 44, 45, 46, 47, 48, 49, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 170, 171, 172, 173, 174, 175) is configured for being coupled with the input port and for transforming the light beam in a structured illumination pattern outside the substrate (10, 30), the pattern having a cartesian component orthogonal to the substrate (10, 30).
Owner:POLITECNICO DI MILANO +1
Down lamp structure with air purification function and air purification system
ActiveCN223435135UDispersed particle filtrationLighting elementsStructural engineeringMechanical engineering
The down lamp structure with the air purification function comprises a panel, an illuminating lamp assembly, a first installation base, a photocatalyst filter element, a second installation base, a draught fan, a rear cover and the like, the panel, the first installation base, the second installation base and the rear cover form a shell structure, the illuminating lamp assembly is arranged on the panel and used for illuminating, and the photocatalyst filter element is arranged on the shell structure. The photocatalyst filter element is matched with the draught fan to form an air purification structure located in the shell. According to the down lamp structure with the air purification function, the air disinfection structure and the down lamp structure are combined, the down lamp structure capable of conducting illumination and air purification is obtained, and the whole structure can be installed at the original installation position used for installing a down lamp; additional arrangement of a new installation position and installation wiring is not needed, the system can be perfectly adapted to new and old buildings, and no additional space is occupied; the whole down lamp structure is simple in structure, convenient to install and maintain and high in practicability.
Owner:BENCHMARK SMART LIGHTING CO LTD
Lighting device
ActiveCN223814619ULight source combinationsLighting support devicesMechanical engineeringMaterials science
Owner:QIANHAI QIANYI SHENZHEN TECH CO LTD
METHOD, DEVICE, LOCATION MICROSCOPE AND COMPUTER PROGRAM FOR LOCATION OF EMISSIONERS IN A SAMPLE
The invention relates to a method for localizing emitters (E) in a sample (2), wherein the sample (2) is illuminated with structured illumination light (B) to localize an emitter (E), wherein after at least one localization of an emitter (E), it is checked whether an accuracy criterion is met, wherein the accuracy criterion indicates whether the position of the emitter (E) is known with a predetermined expected accuracy, wherein a check is carried out using the position of the emitter (E) determined in the localization if the accuracy criterion is met, wherein during the check information about the emitter (E) or the environment of the emitter (E) is collected, wherein the information is taken into account in at least one evaluation step following the localization or in at least one further localization of an emitter (E), and a device,a localization microscope (1) and a computer program for carrying out the procedure.
Owner:ABBERIOR INSTR GMBH
Marine speed reducer gear transmission device
The invention relates to the technical field of speed reducer equipment, in particular to a marine speed reducer gear transmission device which comprises a bottom plate, a main body structure, an observation structure, an illumination structure, an adjusting structure, a calibration structure, a motor and a coupler. Effective speed reduction transmission is achieved through the main body structure, the requirement of ship operation for power output is met, the structure is stable, and the transmission efficiency is high; due to the design of the observation structure, the interior of the speed reducer is convenient to observe, the sealing performance is good, meanwhile, the sealing plate is convenient to mount and dismount, and maintenance is convenient and fast; the illumination structure is matched with the observation structure for use, so that sufficient illumination can be provided for internal observation under the condition of insufficient light, and the observation is clearer and more accurate; through the arrangement of the adjusting structure and the calibration structure, accurate adjustment and rapid calibration of the position of the motor are achieved, and the efficiency and convenience of coaxiality calibration adjustment between the motor and the transmission shaft are improved.
Owner:TAIZHOU JIFENG TRANSMISSION TECH CO LTD
Computer-generated hologram reconstructing device and its structured illumination calibration method and program
To provide a device, method and program which can highly accurately calibrate a structured illumination image of animation CGH.SOLUTION: A camera 10 images an interference fringe region in which interference fringes are spatially multiplexed on a hologram surface 60 and an irradiation region of a structured illumination image irradiated to the hologram surface 60 by a projector 50. An observation image input unit 20 acquires images of the interference fringe region and the irradiation region of the structured illumination image captured by the camera 10 and temporarily stores them. An irradiation region decision unit 30 recognizes the position of the interference fringe region in a pixel coordinate system on the projection side on the basis of a camera image of the interference fringe region and decides the position of the interference fringe region as a target irradiation region of the structured illumination image. An irradiation adjustment unit 40 discriminates the irradiation region of the structured illumination image on the hologram surface 60 on the basis of the camera image and adjusts (calibrates) a projection condition of the structured illumination image with the pixel coordinate system such that the structured illumination image is mapped on the target irradiation region.SELECTED DRAWING: Figure 1
Owner:KDDI CORP +1
Optical image processing
An apparatus (2) for use in optical image processing is disclosed. The apparatus (2) comprises a medium (7) capable of supporting overlapping optical modes (10) such that in response to a spatially structured illumination (4), a set of overlapping optical modes (10) in the medium (7) are non-uniformly excited and output a spatially structured response (6).
Owner:IMPERIAL COLLEGE INNVOATIONS LTD
Lighting lamp structure, lighting assembly, and vehicle
PCT designated stageWO2026016724A1Lighting support devicesVehicle interior lightingMechanical engineeringStructured illumination
The present application relates to the technical field of vehicle lighting, and discloses a lighting lamp structure (20), a lighting assembly (10), and a vehicle. The lighting lamp structure (20) comprises: a lamp body (200), the light exit end of which is provided with a panel (210), wherein the lamp body (200) passes through a mounting hole (110), the panel (210) is located on one side of a first side surface of a mounting member (100), a protruding edge (212) is provided along the periphery of the panel (210), a first limiting structure is provided on one side of the lamp body (200), a second limiting structure (220) is provided on the other side of the lamp body (200), the first limiting structure (220) cooperates with the protruding edge (212) to define a first limiting slot (221) into which the mounting member (100) snaps, and the second limiting structure (230) cooperates with the protruding edge (212) to define a second limiting slot (231) into which the mounting member (100) snaps.
Owner:CHINA FAW CO LTD
Structured illumination of a sample
This application relates to structured illumination of a sample. A system includes a light source, a first grating and a second grating, and at least one reflecting component that, in a first position, forms a first optical path originating from the light source and extending to the first grating and then to a subsequent component in the system, and that, in a second position, forms a second optical path originating from the light source and extending to the second grating and then to the subsequent component.
Owner:ILLUMINA INC
System and method for smart manufacturing quality control with few-shot visual reasoning
PendingUS20260079453A1Adaptive controlProgramme total factory controlPattern recognitionQuality control
The invention discloses a system and method for smart manufacturing quality control with few-shot visual reasoning, wherein the system integrates optical sensing, structured illumination, and adaptive calibration with an artificial intelligence-based visual reasoning architecture. The system comprises a physical inspection device equipped with an adaptive optical sensing unit, a structured illumination unit, an embedded artificial intelligence processing unit, and an adaptive calibration unit. The artificial intelligence processing unit executes a few-shot visual embedding technique that generates feature representations from limited labeled samples. These feature embeddings are structured into a relational graph. A graph attention-based reasoning processor performs relational inference over this graph to identify defect type, severity, and spatial context with minimal training data. The adaptive calibration unit continuously monitors environmental conditions such as illumination, vibration, and temperature, and autonomously adjusts camera exposure, focus, and illumination intensity.
Owner:SINGH SANGEETA +2
Optical image processing
An apparatus (2) for use in optical image processing is disclosed. The apparatus (2) comprises a medium (7) capable of supporting overlapping optical modes (10) such that in response to a spatially structured illumination (4), a set of overlapping optical modes (10) in the medium (7) are non-uniformly excited and output a spatially structured response (6).
Owner:IMPERIAL COLLEGE INNVOATIONS LTD +2
High dynamic structured illumination topography measurement method and device based on pixel-by-pixel regulation
This invention belongs to the technical field of surface morphology measurement, and discloses a method and apparatus for measuring morphology based on pixel-by-pixel high dynamic structured illumination. The method includes: S1: calibrating the DMD and camera to obtain the grayscale response relationship and coordinate mapping relationship between the DMD and the camera; S2: correcting the phase-shifting fringes generated by the DMD using the grayscale response relationship, coordinate mapping relationship, and optical transfer function; S3: projecting the corrected phase-shifting fringes onto the sample surface and acquiring a phase-shifted structured illumination image by the camera; S4: performing slicing imaging on the phase-shifted structured illumination image to extract the three-dimensional morphology of the sample surface. This application can improve the imaging quality of the camera pixel by pixel, and then obtain the three-dimensional morphology of the sample surface by optical slicing multiple high-quality structured illumination images.
Owner:HUAZHONG UNIV OF SCI & TECH
Microscope and microscopy method
The invention relates to a microscope and a microscopy method. The microscope includes: a light source for supplying illumination light; an illumination beam path for directing illumination light to a sample space, the sample space comprising at least one light manipulation device for generating structured illumination light; a detector for detecting emission light emitted by the sample in the sample space; detecting a beam path comprising a microscope objective for directing the emitted light to a detector; and a control unit configured for collecting and evaluating the measurement data Ck from the detector. According to the invention, the control unit is configured to perform the following steps: a measurement data collection step in which measurement data Ck is collected from the detector while the sample is sequentially illuminated with at least two different sample illumination patterns Jk; and a sample information calculation step in which microscopic sample information S is calculated that minimizes or maximizes a scalar cost function L = D [rho (Jk * S), rho (Ck)], where rho is a measure of statistical deviation with respect to the sample illumination pattern index k, and D is a distance measure.
Owner:CARL ZEISS MICROSCOPY GMBH
Wide-field optical slice imaging method, device and equipment based on virtual illumination modulation
The invention relates to the technical field of optical imaging, and provides a wide-field optical section imaging method based on virtual illumination modulation, and the method comprises the steps: obtaining an initial wide-field illumination image and a modulation stripe image of the initial wide-field illumination image, and carrying out the virtual illumination modulation according to the modulation stripe image and the initial wide-field illumination image, obtaining a structural illumination image corresponding to the initial wide-field illumination image, obtaining high-frequency information and low-frequency information corresponding to the initial wide-field illumination image according to the initial wide-field illumination image and the structural illumination image, and obtaining the wide-field illumination image based on weighted fusion of the high-frequency information and the low-frequency information. And obtaining a wide-field optical section tomographic image corresponding to the initial wide-field illumination image. By means of the mode, use of an optical modulation device is avoided, the wide-field optical section tomography effect is achieved only through one initial wide-field illumination image, the wide-field optical section imaging efficiency is improved, the problem that a traditional optical section tomography technology is affected by tissue scattering is solved, and the wide-field optical section imaging quality is improved.
Owner:XIAMEN UNIV OF TECH
Methods for evaluating structured illuminated microscope images and microscopes with structured illumination
2.1. When evaluating microscope images illuminated in a structured manner in different phases, an intermediate result image (f) is obtained. r ) with increased resolution based on the raw images (gi) using a SIM reconstruction method, especially when using a common reconstruction method (jSIM), unmodulated contributions (wide-field contributions) produce artifacts that reduce the contrast of the intermediate result image (f r ) reduce. Such artifacts remain even with jSIM using order-selective filtering. The invention aims to suppress artifacts more effectively. 2.2 By removing elements of the intermediate result image (f r ) are weighted based on elements of the cross-sectional image, and the intermediate result image weighted in this way (f r By outputting the weighted elements as a result image (f̃), artifacts can be effectively reduced or suppressed. It is particularly advantageous to generate a cross-sectional image (f) before weighting. os) of the sample using a reconstruction method and the weighting based on elements of the cross-sectional image (f os to carry out. 2.3. Fluorescence microscopy
Owner:CARL ZEISS MICROSCOPY GMBH
Optical configuration for high resolution microscopy
The invention relates to an optical configuration for high resolution microscopy. An optical configuration for a confocal structured illumination Raman (C-SIM Raman) microscopy system is provided. One example includes: a light source configured to project light; a first set of optical components fixedly aligned along a first optical path, where the first set of optical components includes a vortex phase plate and a quarter wave plate, where the first set of optical components is configured to receive the light; and a second set of optical components configured to be adjusted between a first operating position and a second operating position. When in the first operational position, the second set of optical components is aligned on the first optical path and is configured to expand the light into expanded light in conjunction with the first set of optical components. When in the second operational position, the second set of optical components are not aligned on the first optical path.
Owner:THERMO ELECTRONICS SCI INSTR LLC
Advertising lamp box with advertising board convenient to replace
The invention relates to the technical field of advertising equipment, in particular to an advertising lamp box convenient for replacing an advertisement board, which comprises a lamp box body structure, an illuminating lamp and two groups of advertisement board mounting structures, advertisement watching windows are arranged on two sides of a box body outer cover of the lamp box body structure, and a replacement in-out mechanism is arranged on the side surface of the lamp box body structure; the mechanism is composed of sealing arc-shaped plates fixed through bolts and is convenient to open and close, the advertisement board installation structure comprises two sets of rotating shafts which are driven by a motor through a chain to rotate synchronously, the rotating shafts are sleeved with transmission installation belts, and advertisement board installation groove pieces are fixed to the outer sides of belt bodies. And a plurality of groups of advertisement board limiting mechanisms consisting of springs, adjusting columns and fixing discs are arranged in the groove piece and are used for quickly clamping advertisement boards. Through the side opening type replacement mechanism and the spring limiting mechanism, rapid and safe replacement and reliable fixation of the advertisement board are achieved; the transmission belt is driven by the motor, multi-picture automatic carousel is achieved, and the automatic carousel has excellent sealing performance and is particularly suitable for outdoor advertisement display.
Owner:BEIJING BAISHENG FAMOUS CRAFTSMAN LOGO PROD CO LTD
Structured light digital speckle interference device and method capable of realizing in-plane deformation measurement
The invention discloses a structured light digital speckle interference device and method capable of realizing in-plane deformation measurement, the device comprises a structured light illumination generation module, an imaging module and a data processing module, the structured light illumination generation module comprises a laser, a spatial light modulator, a TIR prism and a spatial mask plate, and the spatial light modulator is connected with the imaging module. The spatial light modulator is used for loading stripe modulation patterns which are in the first direction and the second direction and have different phase shift amounts so as to modulate the illumination light beam, multiple diffraction light beams propagating in the different directions are formed, and phase shift operation is achieved. The spatial mask plate only selects + 1st and-1st diffraction order light beams for interference so as to generate structural illumination light; the imaging module is used for recording corresponding digital speckle phase shift images under the structural illumination light with different directions and different phase shift amounts; and the data processing module is used for obtaining deformation information of the sample along different in-plane directions. The in-plane deformation measuring device can realize high-precision in-plane deformation measurement, is slightly influenced by environmental disturbance, and is compact and high in stability.
Owner:XIDIAN UNIV
Visual inspection system for hub baking varnish quality
The invention belongs to the technical field of hub production, and particularly relates to a hub baking varnish quality visual detection system, which comprises S1, a transmission and positioning module for stably transmitting a hub to be detected to a detection station and clamping and rotating, and S2, a multi-modal data acquisition module for synchronously acquiring multi-dimensional information of the surface of the hub, the module comprises a high-resolution 2D visual unit, a high-precision 3D contour scanning unit and a dynamic structured illumination unit. According to the visual detection system for the hub baking varnish quality, the high-resolution 2D visual unit, the high-precision 3D contour scanning unit and the dynamic structured illumination unit are combined, multi-dimensional information of the surface of a hub is synchronously collected, illumination compensation and reflection elimination are performed on 2D images in different illumination modes by using a Phong illumination model and a pixel value compensation algorithm, and the hub baking varnish quality is detected. A standard image without reflection distortion is generated, clean and reliable input data is provided for subsequent defect recognition, and the misjudgment and omission ratio is remarkably reduced.
Owner:ANHUI TONGJIU TECH CO LTD
Three-dimensional shape data generation apparatus
To suppress an error between measurements when a plurality of workpieces having the same shape are measured. A three-dimensional shape data generation apparatus includes: a storage unit that stores a plurality of measurement files in which each of measurement conditions of a workpiece and each of alignment images are associated with each other; a display control unit that causes a display unit to display a live image of the workpiece and an alignment image associated with one measurement file from among the plurality of measurement files stored in the storage unit; a reception unit that receives a measurement start instruction of the workpiece; and a measurement control unit that controls a structured illumination unit and an imaging unit based on the measurement condition associated with the one measurement file in response to the measurement start instruction.
Owner:KEYENCE CORP
A near-infrared fluorescent probe and a preparation method and application thereof
The application provides a near-infrared fluorescent probe and a preparation method and application thereof, and belongs to the technical field of fluorescent probes.The application takes flavones and heterocyclic compounds as asymmetric molecular building blocks, and controls the electric property, steric hindrance and water solubility of the molecular building blocks through a molecular engineering strategy to prepare the near-infrared fluorescent probe.The near-infrared fluorescent probe has the advantages of long fluorescent emission wavelength, large stokes shift and the like, and effectively avoids the interference of biological background fluorescence.In the aspect of nucleic acid labeling, the near-infrared fluorescent probe has the significant advantages of high signal-to-noise ratio, good selectivity and good biocompatibility.The near-infrared fluorescent probe is imaged by using a laser confocal microscope and a structured illumination super-resolution fluorescence microscope, and the RNA in HeLa cells and mouse white blood cells is successfully traced and displayed, and the fine structure of nucleoli can be visualized at a high resolution.
Owner:EAST CHINA UNIV OF SCI & TECH
Online detection device and method for crystal bar defects
The invention discloses an on-line detection device and method for crystal bar defects, the on-line detection device comprises a shell, a conveying structure, a lighting assembly, a 2D detection assembly, a 3D detection assembly and a computer, a through hole is formed in the thickness direction of the shell, the conveying structure is horizontally arranged in the through hole and is used for placing a to-be-detected crystal bar, and the lighting assembly is used for lighting the to-be-detected crystal bar. The lighting assembly is arranged in the shell and located over the conveying structure, the 2D detection assembly comprises an infrared light source and a linear array industrial camera, the infrared light source and the linear array industrial camera are symmetrically arranged in the shell along a crystal bar, the 3D detection assembly comprises four area array industrial cameras, the four area array industrial cameras are arranged in the shell in the circumferential direction of the crystal bar, and the four area array industrial cameras are arranged in the shell in the circumferential direction of the crystal bar. Each area array industrial camera is aligned with one chamfer edge of the crystal bar, the computer is arranged in the shell, and the computer is connected with the conveying structure, the lighting assembly, the 2D detection assembly and the 3D detection assembly.
Owner:BEIJING WAVE NEW TECH CO LTD
Microscope and microscopy techniques
The invention relates to a microscope comprising a light source for providing excitation light, an illumination beam path for directing the excitation light to a sample chamber, wherein the illumination beam path includes a light manipulation unit for generating a structured illumination pattern in the sample chamber and an illumination objective for directing the excitation light into the sample chamber, at least one drive for changing the illumination pattern with respect to a sample in the sample chamber, a camera with a plurality of camera pixels for detecting emission light emitted by the sample in the sample chamber, a detection beam path comprising a microscope objective for directing the emission light to the camera, and a control unit for evaluating measurement data from the camera.The microscope is characterized in that at least some of the camera pixels are asynchronous camera pixels and that the control unit is configured to read an asynchronous measurement data stream from the camera during changes in the illumination pattern with respect to the sample and to generate image data from the asynchronous measurement data stream from the camera. The invention further relates to a microscopy method.
Owner:CARL ZEISS MICROSCOPY GMBH