35 results about "Structured illumination" patented technology

The invention provides a mounting structure, a lighting system and exposure equipment, the mounting structure comprises a first mounting assembly fixed on a bottom plate, a second mounting assembly movably arranged on the bottom plate, and a flexible connecting piece connecting the first mounting assembly and the second mounting assembly, the first mounting assembly clamps one end of a dodging rod, and the second mounting assembly clamps the other end of the dodging rod. The second installation assembly clamps the other end of the light uniformizing rod, and the equivalent rigidity of the flexible connecting piece is smaller than the clamping rigidity of the first installation assembly and smaller than the clamping rigidity of the second installation assembly. Therefore, when the light homogenizing rod generates thermal deformation due to the change of the environment temperature caused by illumination in the illumination system, the light homogenizing rod can be ensured to generate thermal deformation only in the direction from the first mounting assembly to the second mounting assembly through the single-degree-of-freedom characteristic (namely, the flexible deformation in the axial direction of the light homogenizing rod) of the flexible connecting piece; the light-emitting surface on the light-homogenizing rod is prevented from deviating after thermal deformation, and the working index of the lighting system is ensured.

A self-supervised multimodal structured illumination microscopic reconstruction method comprises: exciting a biological sample with structured illumination to obtain J raw fluorescence image sequences, wherein J is an integer greater than or equal to 1, each raw fluorescence image sequence comprises S fluorescence images, S being an integer greater than or equal to 2; generating a training set for each raw fluorescence image sequence among the J raw fluorescence image sequences; training a denoising neural network on the basis of the training set; and performing super-resolution reconstruction on the S fluorescence images in each of the J raw fluorescence image sequences using a standard structured illumination super-resolution reconstruction algorithm to form a super-resolution image, which is input into the denoising neural network to obtain a final super-resolution reconstructed image.

Systems and methods for image reconstruction are provided. The method may include: implementing a first thread to acquire image data of an object by imaging the object in at least one structured illumination direction using an imaging device, and storing the image data into a buffer; implementing a second thread to obtain one or more image sequences from the buffer; and / or implementing a third thread to extract at least one set of raw images from the one or more image sequences, and transmit the at least one set of raw images to a graphics processing unit (GPU) of the one or more processors, to facilitate the GPU to generate a target image based on the at least one set of raw images, each set of the at least one set of raw images corresponding to one of the at least one structured illumination direction.

The utility model discloses a self-adaptive lighting headlamp mechanism of an electric motor car, and relates to the technical field of lighting lamps. A self-adaptive lighting headlamp mechanism of an electric locomotive comprises an electric locomotive shell, a lamp mounting plate, a lighting lamp and a lighting adjusting structure, four fixing lugs are fixedly connected to the outer surface of the lamp mounting plate and fixedly mounted on the electric locomotive shell through bolts, and the lighting lamp is mounted on the lamp mounting plate. The lighting adjusting structure is located on the lamp mounting plate and comprises a fixing plate, a fixing box, two rotating shafts and a rotating plate, the light sensation sensor capable of being turned over is arranged on the headlamp, when the headlamp is turned on, the light sensation sensor automatically turns over to an effective sensing position, the headlamp is automatically controlled to be turned off in the daytime, and the lighting adjusting structure can be automatically turned off. Therefore, the unnecessary turn-on time of the headlamp is reduced, the working time of the bulb and related circuits is shortened, the aging speed is slowed down, and the service life of the headlamp is further prolonged.

The invention relates to a method for localizing emitters (E) in a sample (2), wherein the sample (2) is illuminated with structured illumination light (B) to localize an emitter (E), wherein after at least one localization of an emitter (E), it is checked whether an accuracy criterion is met, wherein the accuracy criterion indicates whether the position of the emitter (E) is known with a predetermined expected accuracy, wherein a check is carried out using the position of the emitter (E) determined in the localization if the accuracy criterion is met, wherein during the check information about the emitter (E) or the environment of the emitter (E) is collected, wherein the information is taken into account in at least one evaluation step following the localization or in at least one further localization of an emitter (E), and a device,a localization microscope (1) and a computer program for carrying out the procedure.

An apparatus (2) for use in optical image processing is disclosed. The apparatus (2) comprises a medium (7) capable of supporting overlapping optical modes (10) such that in response to a spatially structured illumination (4), a set of overlapping optical modes (10) in the medium (7) are non-uniformly excited and output a spatially structured response (6).

This application relates to structured illumination of a sample. A system includes a light source, a first grating and a second grating, and at least one reflecting component that, in a first position, forms a first optical path originating from the light source and extending to the first grating and then to a subsequent component in the system, and that, in a second position, forms a second optical path originating from the light source and extending to the second grating and then to the subsequent component.

The invention discloses a system and method for smart manufacturing quality control with few-shot visual reasoning, wherein the system integrates optical sensing, structured illumination, and adaptive calibration with an artificial intelligence-based visual reasoning architecture. The system comprises a physical inspection device equipped with an adaptive optical sensing unit, a structured illumination unit, an embedded artificial intelligence processing unit, and an adaptive calibration unit. The artificial intelligence processing unit executes a few-shot visual embedding technique that generates feature representations from limited labeled samples. These feature embeddings are structured into a relational graph. A graph attention-based reasoning processor performs relational inference over this graph to identify defect type, severity, and spatial context with minimal training data. The adaptive calibration unit continuously monitors environmental conditions such as illumination, vibration, and temperature, and autonomously adjusts camera exposure, focus, and illumination intensity.

An apparatus (2) for use in optical image processing is disclosed. The apparatus (2) comprises a medium (7) capable of supporting overlapping optical modes (10) such that in response to a spatially structured illumination (4), a set of overlapping optical modes (10) in the medium (7) are non-uniformly excited and output a spatially structured response (6).

This invention belongs to the technical field of surface morphology measurement, and discloses a method and apparatus for measuring morphology based on pixel-by-pixel high dynamic structured illumination. The method includes: S1: calibrating the DMD and camera to obtain the grayscale response relationship and coordinate mapping relationship between the DMD and the camera; S2: correcting the phase-shifting fringes generated by the DMD using the grayscale response relationship, coordinate mapping relationship, and optical transfer function; S3: projecting the corrected phase-shifting fringes onto the sample surface and acquiring a phase-shifted structured illumination image by the camera; S4: performing slicing imaging on the phase-shifted structured illumination image to extract the three-dimensional morphology of the sample surface. This application can improve the imaging quality of the camera pixel by pixel, and then obtain the three-dimensional morphology of the sample surface by optical slicing multiple high-quality structured illumination images.

The invention relates to a microscope and a microscopy method. The microscope includes: a light source for supplying illumination light; an illumination beam path for directing illumination light to a sample space, the sample space comprising at least one light manipulation device for generating structured illumination light; a detector for detecting emission light emitted by the sample in the sample space; detecting a beam path comprising a microscope objective for directing the emitted light to a detector; and a control unit configured for collecting and evaluating the measurement data Ck from the detector. According to the invention, the control unit is configured to perform the following steps: a measurement data collection step in which measurement data Ck is collected from the detector while the sample is sequentially illuminated with at least two different sample illumination patterns Jk; and a sample information calculation step in which microscopic sample information S is calculated that minimizes or maximizes a scalar cost function L = D [rho (Jk * S), rho (Ck)], where rho is a measure of statistical deviation with respect to the sample illumination pattern index k, and D is a distance measure.

2.1. When evaluating microscope images illuminated in a structured manner in different phases, an intermediate result image (f) is obtained. r ) with increased resolution based on the raw images (gi) using a SIM reconstruction method, especially when using a common reconstruction method (jSIM), unmodulated contributions (wide-field contributions) produce artifacts that reduce the contrast of the intermediate result image (f r ) reduce. Such artifacts remain even with jSIM using order-selective filtering. The invention aims to suppress artifacts more effectively. 2.2 By removing elements of the intermediate result image (f r ) are weighted based on elements of the cross-sectional image, and the intermediate result image weighted in this way (f r By outputting the weighted elements as a result image (f̃), artifacts can be effectively reduced or suppressed. It is particularly advantageous to generate a cross-sectional image (f) before weighting. os) of the sample using a reconstruction method and the weighting based on elements of the cross-sectional image (f os to carry out. 2.3. Fluorescence microscopy

To suppress an error between measurements when a plurality of workpieces having the same shape are measured. A three-dimensional shape data generation apparatus includes: a storage unit that stores a plurality of measurement files in which each of measurement conditions of a workpiece and each of alignment images are associated with each other; a display control unit that causes a display unit to display a live image of the workpiece and an alignment image associated with one measurement file from among the plurality of measurement files stored in the storage unit; a reception unit that receives a measurement start instruction of the workpiece; and a measurement control unit that controls a structured illumination unit and an imaging unit based on the measurement condition associated with the one measurement file in response to the measurement start instruction.

This invention discloses a defect detection device and method based on phase differentiation. The device includes an coded illumination module, a microscopic imaging module, a motion platform module, and a signal detection module. The method uses striped structured illumination to perform multi-frame phase-shift imaging of the surface under test, calculates the complex amplitude, extracts phase information, and performs high-order differential calculations on the phase to achieve simultaneous super-resolution detection of intensity-type and phase-type defects. The advantages of this invention are: it utilizes a structured illumination defect detection device to acquire images and construct complex amplitudes; combined with high-frequency signals amplified and extended by high-order phase differentiation, it overcomes the diffraction limit of the optical system; it also acquires sensitivity to phase-type defects; and it eliminates the need for complex spectral separation and image reconstruction algorithms, significantly reducing system complexity and data processing time.

A backlight module includes a lighting board, a light guide panel, a shielding sheet, and a protrusion structure. The lighting board includes two non-intersected traces, a plurality of microstructure regions and at least one illuminant unit. The illuminant unit is connected between the microstructure regions. The microstructure regions do not overlap with the two non-intersected traces. The light guide panel is disposed above the lighting board, and the shielding sheet is disposed above the light guide panel. The protrusion structure is formed on the shielding sheet, protrudes towards the lighting board, and is between the microstructure regions. The protrusion structure diffuses light of the illuminant unit at an early section of its light path to enter into the light guide panel for lateral transmission. The plural microstructure regions reflect and recycle the light at a following section of the light path to jointly enhance the uniformity of illuminating a keycap.

The application discloses an up-conversion structured light illumination three-dimensional super-resolution imaging system and method, and belongs to the field of microscopic imaging. Light emitted by a laser light source is transmitted into a Fourier lens group to become diffraction light spots of various orders after phase control by a reflective spatial light modulator, and light of diffraction orders of +1 and +2 is filtered out by a light filtering pinhole. The light of diffraction orders of +1 and +2 is irradiated to a sample on an objective table by a microscope objective, and the sample generates a fluorescence band after being excited. The fluorescence is focused to the target surface of a first camera to form an image after being focused by a focusing assembly after passing through the microscope objective. A second super-lens modulator performs axial zoom layer scanning on the fluorescence, and a second camera performs focal plane imaging to lock the best focal plane to adjust the focal length of the first camera. The focal plane searching is more stable, and the introduced interference factors are less. Three-dimensional structured illumination light is formed by interference of the diffraction light of +1 and +2 orders, and three-dimensional super-resolution imaging with a lateral resolution of 100 nm and an axial resolution of 300 nm is realized.

The present invention provides a device of photocatalytic redox reaction based on structured light illumination, comprising: a computer module, sending control instructions to structured light generation module and image acquisition module, to control generations of structured illumination field and total internal reflection illumination field, and acquisition of images of single-molecule fluorescence; the structured light generation module, comprising lasers, an acousto-optic tunable filter (AOTF) and a spatial light modulator (SLM), and controlling the lasers, the AOTF and the SLM to generate and regulate the structured illumination field for exciting a photocatalytic redox reaction, and generate and regulate the total internal reflection illumination field for exciting the single-molecule fluorescence; and, the image acquisition module, acquiring images of fluorescence signals in a process of the photocatalytic redox reaction.

The invention discloses a structured illumination obvious micro-imaging method based on high modulation degree coefficient mapping, and the method comprises the steps: constructing a mathematical model of the Euclidean distance between the positive and negative first-order poles of an illumination structured light spectrum and the original point of the spectrum, and the light frequency of a stripe structure; the whole pixel position of a fringe frequency spectrum pole is estimated only through the structured light fringe frequency and the illumination direction angle, illumination parameter dimensionality reduction is achieved through a method based on principal component analysis, interference components irrelevant to illumination vectors in actual imaging are removed, a first principal component of a core in illumination parameters is obtained, and the first principal component of the core in the illumination parameters is obtained. Therefore, the transverse illumination wave vector with sub-pixel-level precision can be rapidly and accurately obtained without iteration, the problem of illumination parameter estimation errors caused by the fact that the fringe frequency is increased along with the increase of the super-resolution multiplying power of a DMD-SIM system in the prior art is solved, and the super-resolution multiplying power upper limit and the microscopic imaging capability of the system are improved.

The invention belongs to the field of three-dimensional topography measurement, and particularly discloses a structural illumination microscope surface topography reconstruction method and system based on virtual double differential motion, and the method comprises the steps: carrying out the SIM reconstruction and modulation and demodulation of a scanned and collected structural light image, and obtaining an axial modulation response curve of a full-view-field pixel; respectively and virtually moving the scanning position forwards and backwards for the same axial offset distance along the scanning direction to obtain corresponding virtual front and back focus modulation response curves AMR1 and AMR2; based on the sum of Gaussian functions approximated by AMR1 and AMR2, a virtual double-difference modulation response curve is constructed, and z represents a scanning position; when the virtual double-differential modulation response curve crosses a zero point, the corresponding scanning position is the surface height of the sample, and corresponds to the peak position of AMR; therefore, the surface morphology reconstruction of the sample is realized. According to the invention, rapid and high-precision morphology reconstruction of a complex surface can be realized.

Systems are generally described that include a curved light guide for thin structure illumination. An example system includes a light sub-assembly comprising a curved light sub-assembly backing ring and a plurality of light-emitting diodes (LEDs), each LED of the plurality of LEDs being coupled to the curved light sub-assembly backing ring. The example system also includes a curved light guide having an edge coupled to the light sub-assembly, the curved light guide including a pattern of optical extraction features that distribute light and are positioned on the exterior surface of the curved light guide for uniformly distributing light from the plurality of LEDs. The example system also includes a curved reflector including an exterior surface coupled to an interior surface of the curved light guide, wherein the exterior surface is reflective, and a volumetric diffuser coupled to the exterior surface of the curved light guide.

The utility model provides a three-proofing mobile phone with a flashlight waterproof structure and a lighting function, which relates to the technical field of electronic equipment and comprises a mobile phone shell, a display screen assembly fixed on the surface of the mobile phone shell, a mobile phone lamp groove arranged on the back of the mobile phone shell, a back shell lens fixed on the inner wall of the mobile phone lamp groove, and a sinking groove arranged on the surface of the back shell lens. A lampshade is fixed to the bottom of the back shell lens, waterproof foam is fixed to the bottom of the back shell lens, a rack is fixed to the interior of the mobile phone shell, a lamp holder module is fixed to the upper end of the rack, a lamp is fixed to the interior of the lamp holder module, and the surface of the mobile phone lamp groove is fixedly connected with the bottom of the waterproof foam. The novel waterproof lighting structure is adopted, so that the flashlight can achieve a bright lighting effect even under water. Due to the design, the waterproof performance of the flashlight is improved, the underwater lighting effect of the flashlight is enhanced, and a more reliable and bright lighting tool is provided for underwater operation.

Microscopy methods and devices that use structured illumination are described. An example method includes providing multiple digital raw images of a sample that are recorded sequentially a microscope and obtained by illuminating the sample in different phases with periodically structured illumination light. The method includes, determining, using a structured illumination microscopy (SIM) reconstruction method, an intermediate imaging result for the sample with an increased resolution compared to the raw images, where the resolution is higher than the diffraction-limited resolving power of the microscope. In addition, the above method reduces or suppressed image artifacts.

A defect detection system configured to control an energy source to transmit a deep ultraviolet (DUV) laser light at a wavelength along an incident path at different time periods; direct the laser light from an initial angle of transmission to obtain first angles of incidence of the laser light along the incident path at the different time periods; control a polarizer to generate polarized DUV laser light at the different time periods; obtain far-field images for each of the near-field interaction patterns with an image sensor based on the polarized DUV laser light; compare reference patterns with the far-field images in a neural network model, wherein each of the reference patterns is an image of an expected designed patterned reflector transmitter (PRT) pattern with sub-micron features; and obtain an inference on nanometer scale defective distributions in the PRT based on comparing the far-field images with the reference patterns.

The invention discloses a structured light excitation unit and a plasma structured lighting source, and the structured light excitation unit comprises a transparent substrate; and a first metal layer disposed on the first surface of the transparent substrate, the first metal layer being configured to generate an anti-ermitus conjugate coupling effect. Because the first metal layer can generate an anti-Erhi conjugate coupling effect, localized surface plasmon polaritons can be excited to serve as structured light under the irradiation of light, the illumination light source with the different structures is different from a traditional illumination light source with the different structures, and the spatial resolution limit of the SIM is improved under the condition of low illumination intensity.

The invention relates to the technical field of biological materials, in particular to a fluorescent probe for targeting mitochondrial G4DNA, a preparation method and fluorescence lifetime imaging application. The fluorescent probe for targeting mitochondrial G4DNA has membrane permeability, high light stability, mitochondrial targeting and selective recognition capability on mtDNA, especially mitochondrial G4 quadruplex (mtG4DNA), and can realize real-time analysis of mtDNA abundance, conformation and mtG4DNA dynamic change under the condition of no need of gene manipulation, fixation or amplification. Through combination of stimulated radiation depletion (STED) or structured illumination super-resolution imaging (SIM) and a fluorescence lifetime imaging microscope (FLIM), the probe can provide intensity and lifetime dual signals at the same time, accurately maps mtDNA aggregate remodeling, oxidative damage and three-dimensional spatial-temporal dynamics participated by a G4 structure, and can be used for detecting the fluorescence lifetime of the mtDNA aggregate. A universal and non-invasive imaging platform is provided for researching related mechanisms of mitochondrial dysfunction, senescence and neurodegenerative diseases (such as ALS).

The utility model provides a night visible hand-holding-free breast pump, which relates to the field of breast pumps and comprises a breast pump main body, a feeding bottle and a lighting device, the feeding bottle is fixed on the breast pump main body; the breast pump main body comprises a hollow shell structure; the lighting device is fixed in the shell structure; the lighting device comprises a plurality of lamp strips; the lamp strip can emit light rays which irradiate the inside of the feeding bottle; the feeding bottle is transparent. The nighttime visible breast pump free of holding by hands solves the problems that in the prior art, when a mother uses the breast pump free of holding by hands in the environment with weak light, the milk amount in a milk bottle of the breast pump is difficult to see clearly, and the breast pump is inconvenient to use, and the mother can conveniently use the breast pump under the condition with dark light.

This invention discloses a structured light digital speckle interferometry device and method for in-plane deformation measurement. The device includes a structured light illumination generation module, an imaging module, and a data processing module. The structured light illumination generation module includes a laser, a spatial light modulator, a TIR prism, and a spatial mask. The spatial light modulator is used to load fringe modulation patterns with different phase shifts along a first direction and a second direction, respectively, to modulate the illumination beam, forming multiple diffracted beams propagating in different directions and achieving phase shift operation. The spatial mask only selects +1... st -1 st A diffraction-level beam is interfered with to generate structured illumination light; an imaging module is used to record digital speckle phase-shift images corresponding to structured illumination light with different directions and phase shifts; a data processing module is used to obtain deformation information of the sample along different in-plane directions. This invention can achieve high-precision in-plane deformation measurement, is less affected by environmental disturbances, and has a compact device and high stability.

The utility model discloses a study desk with a front illuminating lamp, which comprises a desk body, a storage structure and an illuminating structure, and is characterized in that the storage structure comprises side plates, a back plate and a partition plate, the side plates and the back plate are vertical and form a semi-enclosed structure, the two side plates are respectively arranged on two sides of the back plate, the partition plate is horizontally arranged and fixed on the side plates and the back plate, and the front illuminating lamp is arranged on the front illuminating lamp. The lighting structure comprises a lighting main body and a connecting part, the lighting main body is fixedly connected with the connecting part, the connecting part is provided with two parts which are located at the two ends of the lighting main body respectively and fixedly connected with the side plates, the connecting part is of an arc-shaped structure extending forwards, and the lighting main body is arranged at the front end of the connecting part; the projection of the lighting body on the horizontal plane is not overlapped with the projection of the partition plate on the horizontal plane. According to the study desk with the front illuminating lamp, through the front illuminating body, it can be guaranteed that the illuminating effect is uniform and bright, the eyesight of a user is protected, meanwhile, the upper portion of the partition plate is not shielded, the storage space of the partition plate is not affected, and the structure is reasonable.

The invention provides a three-dimensional super-resolution microscopic imaging system and method. The three-dimensional super-resolution microscopic imaging system comprises an illumination unit, a phase modulation unit, a light conduction unit and an imaging unit. Wherein the illumination unit is used for outputting uniform and parallel exciting light to the phase modulation unit, the phase modulation unit is used for generating multiple beams of structured light with differentiated axial phases, the light conduction unit is used for precise projection of the structured light and efficient convergence of fluorescence, and multi-axial depth information is integrated in combination with a super-resolution reconstruction algorithm. And finally, obtaining the total-volume optical super-resolution image of the sample to be detected. According to the invention, the collection efficiency of a single volume image can be greatly improved, three-dimensional super-resolution imaging with high transverse resolution, high axial resolution and high fidelity is realized, and the application scene and practical value of the structured illumination obvious micro-imaging technology in the fields of life science and the like are expanded.

The disclosure relates to an imaging method and an imaging system for carrying out this method. The disclosure provides an improved multispectral ptychographic imaging method and a corresponding system. For this, the imaging method of the disclosure comprises the following steps: Generation of at least partially coherent electromagnetic radiation (3) which contains spectral components at at least two different wavelengths; spatial separation of the spectral components of the electromagnetic radiation, for example by angular dispersion; generation of structured illumination beams (9) by selecting respectively one of the spectral components by means of a structured aperture (8a, 8b, 8c); moving an object (6) in a direction transverse to the path of the illumination beams (9) into a plurality of positions; detecting an intensity pattern for each of the positions, wherein the intensity patterns are generated by scattering and / or diffraction of the illumination beams (9) at the object (6) in a detection plane; and reconstructing an image of the object (6) from the detected intensity patterns, wherein for each of the at least two wavelengths an image of the object (6) is calculated by means of a ptychographic reconstruction algorithm.