73 results about "Burn wound" patented technology

The invention discloses a burn wound image detection method based on an attention-enhanced convolutional neural network, and belongs to the field of medical image artificial intelligence target detection. The method comprises the following steps: establishing and marking a burn wound skin image data set, introducing a Swin Transform and an LSKA attention module in series behind a spatial pyramid pooling fast module (SPPF) at a backbone part of a YOLOv11 network, forming an enhanced Backbone of SPPF-Swin-LSKA, and realizing collaborative optimization of global modeling and local detail perception; and cross-layer feature routing and multi-scale prediction layer configuration are redesigned at a Head end so as to improve the detection capability of a small target and the recognition performance under a complex background. The method can effectively reduce missing detection and false detection, improves the detection stability and robustness, and has good real-time performance and clinical application value.

The invention provides a burn wound area segmentation method and system, and the method comprises the steps: obtaining a to-be-segmented image through constructing an anatomy database, and employing a lightweight CNN classifier to extract a feature vector; determining a healing stage of the burn wound according to the feature vector, and adjusting boundary parameters according to the healing stage; inputting an image to be segmented into the improved U-Net encoder network, and outputting a segmentation mask and a boundary probability graph; determining an interaction area according to the boundary probability graph, carrying out interaction in the interaction area by referring to the segmentation mask, and generating a smooth boundary according to interaction data; and post-processing optimization fused with prior constraints is performed on the smooth boundary to obtain a target boundary so as to complete burn wound area segmentation, specific and prior knowledge provides basic constraints for segmentation, interactive operation pertinently corrects a complex area, post-processing realizes global consistency optimization, and a'constraint-correction-optimization 'closed-loop processing flow is formed.

The invention relates to the technical field of temperature mapping, in particular to a burn wound infection early warning method and system based on infrared thermal imaging monitoring, and the method comprises the following steps: extracting an image temperature recombination thermal matrix, recognizing a contour, screening edge fluctuation, trimming a thermal difference to track a heat flow path, calculating a drift trajectory, and analyzing heat value mutation. And a wound infection early warning scheme is obtained. According to the invention, through temperature reconstruction and thermal difference distribution analysis of an infrared image, a wound surface hot area range and a thermal energy gathering trend are accurately identified, dynamic tracking of edge thermal drift is realized in combination with a heat flow path and a heat value change direction, and a multi-layer heat map structure is constructed by using heat value increasing and climbing mutation characteristics. According to the method, the identification precision and infection risk early warning capability of a thermal abnormal region are improved, the sequential expression and spatial structure definition of temperature change are enhanced, the fuzzy problem of a static heat map is improved, and continuous monitoring of the thermal evolution process of a wound surface and directional positioning of a high-risk region are realized.

The formation of scars at a wound site is reduced by contacting the wound site with an effective dose of an inhibitor of focal adhesion kinase (FAK) formulated in a pullulan hydrogel. The release profile of the FAK inhibitor can be adjusted according to the nature of the wound, e.g., excisional wounds, burn wounds, etc.

The invention discloses oil gel based on hermetia illucens oil as well as a preparation method and application of the oil gel, and relates to the technical field of biological medicines. According to the oil gel based on the hermetia illucens oil, the hermetia illucens oil is used as a grease matrix, and a stable lipid network structure is constructed by combining monoglyceride from hermetia illucens and vegetable wax. The hermetia illucens oil is derived from an animal lipid metabolism system, the fatty acid chain length distribution, the polarity characteristic and other aspects of the hermetia illucens oil are highly similar to those of a human skin cuticle lipid system, a continuous lipid covering layer can be formed on the surface of a burn wound, and the wound barrier condition is effectively improved; meanwhile, antioxidant fat-soluble components contained in the hermetia illucens oil are beneficial to reducing the local oxidative stress level of the wound surface and improving the microenvironment of the wound surface. The oleogel provided by the invention is suitable for external nursing of burn wounds, has dual functions of bionic barrier construction and antioxidant synergistic regulation, and can provide a novel and efficient biomedical material for burn wound repair.

The invention provides a traditional Chinese medicine composition for promoting burn wound healing and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine. The traditional Chinese medicine composition comprises the following components in parts by weight: 15-20 parts of fructus cnidii; 20-40 parts of sargentgloryvine stem; 20 to 40 parts of dandelion; 20 to 40 parts of polygonum cuspidatum; 25 to 30 parts of cortex phellodendri; 25 to 30 parts of radix sophorae flavescentis; 10 to 15 parts of Chinese gall; 1-5 parts of a temperature slow release agent; 1 to 5 parts of mucus polysaccharide; wherein the temperature slow-release agent is prepared from menthol glucoside, tris (hydroxymethyl) aminomethane and sodium alginate. The traditional Chinese medicine composition is prepared by compounding fructus cnidii, sargentgloryvine stem, dandelion, polygonum cuspidatum, golden cypress, radix sophorae flavescentis and gallnut, has the efficacy of resisting inflammation, resisting bacteria, promoting healing, astringing to relieve pain and the like, and is combined with the temperature slow-release agent and mucous polysaccharide, so that the temperature required during fumigation and washing is effectively regulated and controlled while the efficacy is promoted, and the fumigation and washing effects are improved. And the secondary injury of the wound surface caused by improper control of the fumigation and washing temperature is avoided.

The present application relates to the field of biotechnology, in particular to the application of Radix Rabdosiae Rubescens derived exosomes in the preparation of a drug for promoting wound healing, wherein the prepared Radix Rabdosiae Rubescens derived exosomes are uniform in size, good in appearance, can promote cell proliferation, have good effects of promoting fibroblast migration and reducing scar formation; and a scar-free wound healing hydrogel containing Radix Rabdosiae Rubescens derived exosomes is also provided, wherein the hydrogel can encapsulate rEVs or ORI, can effectively increase the proportion of type III collagen in the new skin tissue, effectively reduce the formation of scars, effectively promote the healing of deep second-degree burn wounds, and effectively inhibit the MMT process of macrophages in the wound healing process, and has a wide application prospect in the field of scar prevention and treatment.

This invention provides a traditional Chinese medicine composition for treating skin trauma, its preparation method, and its application, relating to the field of traditional Chinese medicine technology. The traditional Chinese medicine composition of this invention comprises the following raw materials in parts by weight: Astragalus membranaceus 20-30 parts, Piper kadsura vine 20-30 parts, Dragon's blood 10-20 parts, Rheum palmatum 3-8 parts, Coptis chinensis 3-8 parts, Phellodendron chinense 3-8 parts, Angelica sinensis 8-15 parts, Panax notoginseng 1-5 parts, Angelica dahurica 5-10 parts, Rehmannia glutinosa 5-10 parts, Scrophularia ningpoensis 3-8 parts, Aucklandia lappa 1-5 parts, Paeonia suffruticosa 5-10 parts, Rosin 3-8 parts, Vespa nidus 10-15 parts, and Carbonized human hair 1-5 parts. The various medicinal materials of this invention interact and complement each other, possessing the effects of promoting diuresis and reducing swelling, promoting tissue regeneration and detoxification, anti-inflammatory and analgesic effects, and activating blood circulation and removing blood stasis. It can reduce tissue swelling and redness, effectively prevent infection of burn wounds, and improve wound healing speed. It has significant therapeutic effects on skin trauma caused by burns and scalds, with no toxic side effects.

The present application belongs to the technical field of medical equipment, and discloses a moisturizing device for burn plastic surgery nursing, which comprises a moisturizing cover, a water tank, a heater, an atomizer, a fan and a power supply. The moisturizing cover is hemispherical and made of transparent plastic, the inner surface of the moisturizing cover is covered with multiple layers of microporous membranes, the outer surface of the moisturizing cover is provided with multiple ventilation holes, the lower end of the moisturizing cover is open and used for being sleeved on a burn wound surface, the upper end of the moisturizing cover is provided with an air inlet and an air outlet; the water tank is used for storing a solution, the lower part of the water tank is provided with a water outlet connected with a water pump, the other end of the water pump is connected with the air inlet of the moisturizing cover through a water pipe, a valve is arranged on the water pipe and used for controlling the size of water flow; the heater is used for heating the solution in the water tank; the atomizer is used for atomizing the solution delivered by the water pump; the fan is used for discharging air and water mist in the moisturizing cover; the power supply is used for providing power for the water pump, the heater, the atomizer and the fan, a switch is arranged on the power supply and used for controlling the on-off of the power supply; and the controller is used for controlling the working state of the moisturizing device.

The application discloses a flagellum inhibiting peptide of pseudomonas aeruginosa and a preparation method and application thereof, and the sequence of the flagellum inhibiting peptide is Lys-Ile-Gly-Leu-Phe-Arg-Trp-Arg. The synthetic inhibiting peptide has a time-dependent self-assembly ability, can gradually evolve from scattered granular into filamentous structure, and finally forms a stable net-like morphology. The synthetic inhibiting peptide can significantly inhibit the flagellum motility of PAO1 and other strains of pseudomonas aeruginosa, and has synergistic antibacterial activity when combined with allicin, ciprofloxacin, meropenem, levofloxacin, polymyxin B and the like. Moreover, the synthetic inhibiting peptide has obvious bacteriostatic and healing-promoting effects on burn wound infection.

The invention relates to a bacteriostatic repair medical gel containing glycosylglycerol and a preparation method thereof, and belongs to the technical field of medical dressings. The gel is prepared by taking glycosylglycerol as a main active component and cooperating with chitosan, collagen, a silver ion antibacterial agent and other components through a specific process. The gel has triple synergistic effects: glycosylglycerol enhances the skin barrier function and provides lasting moisture retention by activating aquaporin; chitosan and silver ions provide broad-spectrum antibacterial activity; and collagen and glucan promote tissue repair and cell proliferation. Experiments show that the antibacterial rate of the gel on common pathogenic bacteria such as staphylococcus aureus and escherichia coli exceeds 99.9%, meanwhile, the wound healing speed can be increased by 40% or above, and the gel is good in biocompatibility and free of skin irritation. The gel can be used for repairing and nursing various skin injuries such as burn wounds, chronic ulcers and surgical wounds.

The present application relates to a kind of zinc-doped antibacterial carbon dots and its preparation method and application, the preparation method of the zinc-doped antibacterial carbon dots is that biomass, anhydrous zinc acetate is dispersed in deionized water according to mass ratio, after ultrasonic stirring, mixed solution is obtained, after hydrothermal reaction, mixed solution is obtained after natural cooling, centrifugal, filtration treatment, carbon dot solution is obtained, carbon dot solution is obtained after freeze-drying treatment, and powder zinc-doped antibacterial carbon dots are obtained.Then zinc-doped antibacterial carbon dots are dissolved in double distilled water with carboxymethyl chitosan, bioactive glass according to proportion, stirring is obtained at room temperature, and composite hydrogel is obtained.The zinc-doped antibacterial carbon dots and composite hydrogel prepared in the present application have good antibacterial effect, the preparation process of composite hydrogel is simple, low in cost, low in cytotoxicity, and zinc-doped antibacterial carbon dots have wide application prospect in the preparation of antibacterial material and the treatment of skin deep lesions, diabetic wound and burn wound dressing material.

This invention belongs to the field of medical pharmaceuticals, specifically relating to a traditional Chinese medicine alcohol dressing and its application method; a traditional Chinese medicine alcohol dressing comprising one of Lithospermum erythrorhizon, borneol, or Polygonum cuspidatum, and alcohol; the weight ratio of one of Lithospermum erythrorhizon, borneol, or Polygonum cuspidatum to alcohol is 1.5–2:10; a method of applying the traditional Chinese medicine alcohol dressing includes the following steps: clearing static electricity; spraying the traditional Chinese medicine and alcohol onto the wound and blisters, avoiding the mouth, eyes, ears, and nose, for rapid pain relief and heat dissipation; preserving the intact wound and blisters; implementing corresponding treatment measures according to the type of burn; discharge from the hospital after treatment and rest at home to allow the biological cells' electromagnetic sensing self-healing ability to recover; during the operation of the aforementioned method, to avoid secondary trauma, the burn wound must never be bandaged or disinfected with iodine; only spraying and application are permitted. This application invents a traditional Chinese medicine alcohol dressing and its application method, which is highly targeted, effectively relieves pain and prevents infection, reduces scar formation, and is suitable for different types of burns.

The invention discloses a modified exosome as well as a preparation method and application thereof, and relates to the technical field of biological medicines, CD44 targeted modification and composite active ingredients are synergistic to realize efficient repair of burn wounds and sensitive skin, and the modified exosome can be specifically enriched on the burn wounds or damaged sensitive skin parts through CD44 targeted peptide fragment coupling, so that the effect of repairing the burn wounds and the sensitive skin is achieved. The defects that a traditional repairing product is poor in targeting property and low in active ingredient utilization rate are overcome, the loaded dipotassium glycyrrhizinate, glutathione and FGF form a synergistic effect, wound healing and skin barrier repairing can be promoted through the FGF, the dipotassium glycyrrhizinate and the glutathione can be used for strongly resisting inflammation and oxidation, release of sensitive skin inflammatory factors is reduced, and the repairing effect is achieved. By matching with nitrogen protection incubation and a pulse ultrasonic loading process, the stability of active ingredients is greatly improved, finally, the repair effects that the healing process of burn wounds is remarkably accelerated, and sensitive skin percutaneous moisture loss is obviously reduced are achieved, and safety and effectiveness are both considered.

The invention discloses a burn wound negative pressure treatment and medicine perfusion combined drainage device which comprises a shell, a negative pressure mechanism, an infusion mechanism, a drainage opening, a supporting mechanism, a pressing mechanism and an adsorption mechanism, a rotating ring is installed at the top of the shell, and a guide column is arranged on the inner wall of the rotating ring; the supporting mechanism is fixed in the shell and provided with a supporting channel, and the negative pressure mechanism penetrates through the supporting channel. A drainage opening is formed in the surface of the shell; the negative pressure mechanism comprises a connecting port, a negative pressure pipeline and a protruding block, and the connecting port is formed in the top of the negative pressure pipeline. Therefore, negative pressure treatment of the wound surface and medicine perfusion can be carried out synergistically, and wound surface infection can be controlled in time; a negative pressure system does not need to be disassembled and assembled in each time of perfusion, operation is greatly simplified, medical care burden is relieved, frequent operation on a wound surface is reduced, damage to new granulation tissue is avoided, pain of a patient is relieved, and the risk of secondary pollution caused by repeated exposure of the wound surface is reduced.

This invention relates to the field of burn wound type identification technology, specifically disclosing a method and system for burn identification and effect prediction based on neural networks, comprising the following steps: S1, acquiring burn wound images and performing preprocessing; S2, constructing a convolutional neural network classification model, employing a transfer learning strategy, and training the convolutional neural network classification model using the preprocessed burn wound images to obtain a trained burn identification model; S3, inputting the burn wound image to be identified into the trained burn identification model, and outputting the burn grade and corresponding thermal damage effect identification results; wherein the burn grades include: first-degree burns, superficial second-degree burns, deep second-degree burns, and third-degree burns; this invention achieves the identification of detailed burn grades and thermal damage effects by constructing and training a convolutional neural network classification model employing a transfer learning strategy.

The invention discloses an artificial intelligence-driven burn wound assessment method and system, and relates to the technical field of medical information processing and intelligent diagnosis, and the method comprises the following steps: S001, employing a fixed imaging attitude to collect continuous short-sequence wound image frames, calculating a brightness change curve of adjacent image frames, generating a light and shade rhythm track, and drawing a beat frequency prompt band, the method is used for marking a light source flicker rule and establishing an initial reference baseline of brightness change. Through brightness breakpoint identification, error shooting parameter calculation, light source phase jitter and inverse rhythm optical shutter regulation and control, active separation and reverse coupling of illumination rhythm and sampling rhythm are realized, periodic flicker interference is effectively eliminated, the brightness stability and texture reduction degree of a wound image are improved, an artificial intelligence evaluation result is more accurate and reliable, and the artificial intelligence evaluation efficiency is improved. And the objectivity and safety of clinical diagnosis are improved.

The invention discloses medical amorphous hydrogel and a preparation method thereof, and relates to the technical field of medical amorphous hydrogel, and the medical amorphous hydrogel is prepared from the following components by mass: 0.2-3% of a gel matrix; 0.1%-0.8% of a thickening agent; 0.8%-5% of a humectant; 0.01%-0.4% of a preservative; 0.2%-0.5% of a neutralizing agent; and the balance of purified water. The amorphous hydrogel provided by the invention can be tightly attached to a burn wound surface, not only can be used for moistening the wound surface and efficiently absorbing or discharging wound surface seepage, but also can be used for absorbing wound surface energy so as to relieve pain and cool skin, and meanwhile, air pollution protection is realized by virtue of a base material, so that an excellent environment is provided for healing of the burn wound surface.

The invention discloses an application device for burn wound repair, and belongs to the technical field of burn application equipment.The application device comprises a body surface application layer, a reagent limiting and releasing layer and a medicine storage layer, the body surface application layer is tightly attached to burn skin, net-shaped small holes are formed in the body surface application layer, the reagent limiting and releasing layer is arranged above the body surface application layer, and the medicine storage layer is arranged above the reagent limiting and releasing layer. The body surface pasting layer is fixedly connected with the reagent limiting and releasing layer, and the medicine storage layer is connected with the reagent limiting and releasing layer. In order to overcome the defects that an existing burn application is inconvenient to change medicine, uneven in medicine absorption, poor in application adaptability and the like, the invention provides the application device for repairing burn wounds, and the problems that secondary trauma is easily caused when a traditional application is replaced, residual medicine affects new medicine effect exertion, and the traditional application is difficult to attach to wounds in different shapes are effectively solved.

The invention relates to a multifunctional biopolymer synthesized from coconut shell and / or coconut shell fiber wood pith powder and application thereof. The present invention relates to the synthesis and production of biopolymers from natural waste-coconut shell and / or coconut shell fiber wood pith powder. More specifically, it relates to a novel multifunctional biopolymer obtained from a copolymer, cross-linking agent and plasticizer combination with coconut shell and / or coconut shell fiber wood pith powder (coconut fruit) for use in the pharmaceutical, nutraceutical, cosmetic and leather industries. Lignin components are extracted and purified from coconut shells, physicochemical characteristics of the lignin components are studied, and the lignin components are processed into various biopolymers to be used as burn wound dressings and leather substitutes.

The invention relates to a shikonin-loaded self-healing hydrogel wound dressing as well as a preparation method and application thereof. The method comprises the following steps: modifying TA on TOCNF to obtain TA-coated TOCNF; and compounding the TA-coated TOCNF with PVA, and sequentially adding an alkannin solution and a borax solution to prepare the hydrogel wound dressing. The hydrogel has the design characteristics that TA with a convergence effect on scalds and burns is introduced, so that the hydrogel is endowed with certain antibacterial activity and adhesion; on the basis of boric acid ester bonds formed by borax and PVA, the hydrogel is endowed with good self-healing property; meanwhile, the hydrogel is loaded with an active small molecular medicine shikonin with a repairing effect on scalds, so that the antibacterial activity and the oxidation resistance of the hydrogel are further enhanced. The preparation method is simple, can well adapt to irregular scald wounds, integrates antibacterial activity and oxidation resistance, and prepares the shikonin-loaded self-healing hydrogel wound dressing which can be produced on a large scale and has a good healing effect on scalds.

The invention relates to the technical field of traditional Chinese medicine preparations, and discloses a traditional Chinese medicine preparation for treating skin scald. The invention discloses a traditional Chinese medicine preparation for treating skin scald. The traditional Chinese medicine preparation sequentially comprises a hydrogel bottom layer, a functional layer and a hydrogel covering layer from bottom to top. The traditional Chinese medicine preparation for treating skin scald provided by the invention has a good wound healing effect, has a remarkable inhibition effect on inflammatory response of tissues around a scald wound, and has an excellent inhibition effect on common pathogenic bacteria on the scald wound.

The invention discloses a preparation method and application of Chinese herbal medicine hydrogel. The preparation method of the Chinese herbal medicine hydrogel comprises the following steps: (1) preparing keratinocyte vesicles by taking keratinocytes as a raw material, then adding asiaticoside, and extruding back and forth through a cellulose acetate membrane to obtain keratinocyte loaded asiaticoside vesicles; and (2) diluting the keratinocyte loaded asiaticoside vesicles with a buffer solution, then adding calcium carbonate powder, then adding atriplex obscura leaf powder, uniformly mixing, and then shaping with a mold to obtain the Chinese herbal medicine hydrogel. According to the Chinese herbal medicine hydrogel, the asiaticoside is combined with the keratinocyte bionic nano-vesicles, so that the medicine can be promoted to better reach the interior of skin cells, and the Chinese herbal medicine hydrogel is particularly suitable for treating burn wounds, diabetic wounds and other wounds difficult to heal.

This disclosure provides the use of molecular complexes of polyammonium bisulfate alone and in combination with certain metals or metal salts to treat burn wounds.

A non-enzymatic debridement agent that is less expensive, less cytotoxic, and more effective than enzymatic and sharp surgical debridement of eschar material. The non-enzymatic debridement agent is based on a formulation of detergent with a high critical micelle concentration and a denaturing agent along with an optional optimized buffer. The non-enzymatic wound debriding agent is able to dissolve eschar material in under one week. The buffer or ointment may contain an optimized pH, salt concentration, reducing agent, and other components that aid in the debridement of eschar material from chronic wounds and burn wounds. The non-enzymatic debridement agent can be formulated as an ointment, liquid rinse under pressure, and / or as a combination product with an advanced wound dressing that can act as a sink to remove the solubilized eschar material from the surface of the wound to assist in the debridment and proper healing of wounds.

This invention discloses an intelligent burn diagnosis system based on multimodal data fusion and four-dimensional assessment, comprising the following steps: collecting multimodal data of burn wounds and preprocessing it; fusing the acquired multimodal data of burn wounds and extracting key features; after completing multimodal data fusion and feature extraction, constructing a four-dimensional burn assessment system including tissue damage index, thermal damage coefficient, infection risk value, and healing potential score; determining the severity of burns based on the four-dimensional burn assessment system; and providing corresponding treatment suggestions based on the output of the burn assessment results. This invention employs the aforementioned intelligent burn diagnosis system based on multimodal data fusion and four-dimensional assessment, integrating multi-source data such as visual images, infrared thermal imaging, and TOF ranging, combined with artificial intelligence analysis technology, to provide more objective and accurate assessment results through automated data acquisition and intelligent analysis, achieving precise assessment of burn wounds.

The invention discloses a burn wound nursing device, and belongs to the technical field of medical instruments, the burn wound nursing device comprises a nursing assembly, a supporting assembly and a base, the nursing assembly and the supporting assembly are arranged on the base, and the supporting assembly is located on the front side of the nursing assembly; when a medical worker pulls a driving rod in a reciprocating mode and pulls the driving rod anticlockwise, a driving ring outer gear drives a lead screw gear to rotate, a lead screw pushes a nursing assembly to move forwards along an inclined bottom groove, meanwhile, a double-gear piece drives a nursing ring to rotate by more than 360 degrees, and medicine is taken while the nursing ring is moved; during clockwise resetting, the lead screw drives the lead screw gear to be disengaged, and repeated medicine feeding is avoided (only the shell needs to be pushed by hands during resetting). The distance of the supporting assembly can be adjusted through a sliding plate, an adjusting screw drives a supporting base to be opened and closed to adapt to arms, shanks and other limbs with different thicknesses, an elastic cushion can reduce hard compression and protect wound surface surrounding tissue and blood circulation, operation is convenient and fast, efficiency is high, and the nursing device is suitable for various burn wound surface nursing scenes.

The present application relates to the technical field of medical auxiliary apparatus, disclose a kind of burn wound medicine application auxiliary system, including operating cylinder and smearing block, and smearing block is detachably connected with operating cylinder.Arch-shaped through-hole is provided in smearing block, and partition is provided in through-hole, and the top of partition is used to store plaster, and partition includes spiral segment, horizontal segment, the end of spiral segment of partition and the adjacent end of through-hole are left with blanking hole, and the bottom port of blanking hole is provided with discharge pipe;Squeezing mechanism is installed on operating cylinder to squeeze the plaster stored in through-hole, so that the plaster is squeezed out from discharge pipe.The present application can facilitate quantitative application of ointment by the above scheme, and one hand can hold operating cylinder to work, improve work efficiency, and use comfortable and convenient.

This invention discloses a traditional Chinese medicine composition for bone setting and burn treatment based on a family secret recipe, and its application. The composition is a powder preparation, processed using a specific technique. For internal use in bone setting and tendon repair, it should be taken with warm yellow rice wine. After taking the medicine, the patient should sweat profusely, with dampness in the wrists and ankles, and strictly avoid foods such as eggs, beef, mutton, donkey, horse, dog meat, old sow, and boar meat until the fracture has clinically healed. For external use in treating burns, the powder should be mixed with sesame oil to form a paste and applied to the wound. No pre-sterilization is required. Blisters should be punctured before applying the medicine. This composition significantly promotes bone healing (cycle 2-8 weeks) and burn wound healing (cycle 1-8 weeks), leaving no scars after burn healing, with a cure rate of over 99.7% and no toxic side effects.

The present application relates to a bacillus subtilis TN, a live bacterial preparation and a preparation method and application thereof, and belongs to the technical field of microorganisms. The bacillus subtilis TN was preserved in the Guangdong Provincial Microbial Culture Collection Center on March 8, 2023, and the strain preservation number is GDMCC No: 63246, and the preservation address is No. 100, Martyr Road, Guangzhou City, Guangdong Province. The bacillus subtilis TN and the live bacterial preparation provided in the present application can effectively avoid the problem of secondary infection of a burn wound caused by an unsuitable bandaging therapy, or the problem of dysbacteriosis and drug resistance caused by the abuse of biological preparations such as antibiotics when the bacillus subtilis TN and the live bacterial preparation are applied to treat a burn wound, and have the effects of anti-inflammation, antioxidant, inhibition of melanin production and moisturizing, are beneficial to promoting wound recovery and preventing scarring, and through compounding with a nutrient solution, the problems of difficulty in survival of the bacillus subtilis when applied to a wound and poor film-forming hemostatic effect are effectively solved.