The invention provides an extraction method of autologous neutrophil physical activation multi-component
protein and application of the autologous neutrophil physical activation multi-component
protein in chronic infectious
wound treatment. According to the method, the neutrophil is physically activated, so that chemical irritant residues are avoided, and the compound
protein component rich in MPO, NE,
Lysozyme,
Lactoferrin and other multiple bactericidal proteins is obtained. And the protein component is directly delivered to the basal part of the
chronic wound surface in a multi-point and layered injection mode (1 time / 3 days, 250 [mu] L / time) in a
focus area, so that bacterial biofilms and necrotic tissues can be effectively removed,
wound debridement,
cell migration and matrix remodeling are promoted, and the healing of the chronic infectious
wound surface is accelerated. The method disclosed by the invention has the advantages of autologous source, good
biocompatibility, accurate treatment and the like, is particularly suitable for treating chronic infectious wounds such as
diabetic foot ulcer and the like, and provides a new thought for treating the chronic wounds.