The invention relates to a preparation and using method of medical biocompatible stent material. The medical biocompatible stent material is prepared by applying the small intestine submucosal and the tendon tissue of people or animals as the raw materials. The invention has good biocompatibility, appropriate porosity and mechanical strength and stable spatial structure, and the invention contains various growth factors to induce the tissue for regeneration and reconstruction, thereby improving the success rate of the repair of the various tissue defects and the reconstruction of tissue structures, therefore, the invention is an ideal tissue engineering material. The medical biocompatible stent material which is provided by the invention is applicable to the preparation of various tissue engineering equipments and the application in the repair of various tissue defects and injuries, including skin, blood vessels, nerves, tendons, bones, cartilages, abdominal walls, urinary tracts, oral mucosa and so on, and the invention can be used in the treatment of trauma, abdominal external hernia, anal fistula, rectovaginal fistula, rectum anterior protrusion, bone and cartilage defects, vascular injuries, nerve injuries, tendon injuries, oral mucosal injuries, relaxed pelvic floor, urinary tract injuries and other diseases.