8 results about "Tendon tissue" patented technology

The invention belongs to the field of tendon tissue scaffold preparation, and provides a tendon tissue scaffold preparation method based on a decellularization technology. Although the existing decellularization technology can realize removal of cell components and retention of a matrix structure, contradiction still exists in the aspects of considering decellularization efficiency and matrix integrity. The method comprises three steps of freeze thawing treatment, trypsin digestion ultrasonic treatment and nuclease treatment, and perfect balance between decellularization efficiency and matrix retention is realized by optimizing freeze thawing parameters, regulating ultrasonic conditions and reasonably matching enzyme types and concentrations. According to the method, the cell structure can be accurately destroyed, the release of cell contents is promoted, and meanwhile, the extracellular matrix structure and bioactive molecules are protected. Experiments show that the tendon tissue scaffold prepared by the invention is thorough in cell removal, retains an extracellular matrix structure of natural tendons to the greatest extent, is closer to natural tissues in mechanical properties and biological functions, and provides a new thought for tendon repair research and application.

The invention relates to the technical field of medical surgery, and discloses an improved parachute technology and bone marrow stimulation combined rotator cuff repair method, which comprises the following steps: establishing at least five operation admission paths: a posterior lateral admission path, an anterior lateral admission path, a far-end anterior lateral admission path, a posterior lateral front admission path and a BMS (Battery Management System) and anchor channel admission path, and bone marrow stimulation operation is carried out in the rotator cuff footprint area, specifically, a drill bit with the diameter being 2 mm is used for drilling holes with the hole depth being 5 mm and the interval being 5 mm till bone marrow seeps out, and at least one inner side anchor nail is placed at the position about 2 mm away from the outer edge of the joint cartilage of the humerus head and close to the front edge of the rotator cuff tear. According to the improved parachute technology and bone marrow stimulation combined rotator cuff repairing method, the improved Mason-Allen suture bridge technology and the bone marrow stimulation technology are organically combined, a parachute suture structure is introduced, double optimization of mechanical stability and biological healing is achieved, meanwhile, the parachute structure only needs three times of threading, the threading path is clear, the operation is simple, and the operation is convenient. The operation difficulty is obviously reduced, the operation time is shortened, and the iatrogenic injury to the tendon tissue is reduced.

Surgical mesh material, especially for use in hernia surgery, comprising biological tendon tissue and manufacturing processes therefor, and the use of a mesh graft device.

The application provides a high-strength high-biocompatibility tough hydrogel and a preparation method and application thereof. The preparation method is a method of repeatedly stretching in a sulfate solution and soaking in a PBS buffer solution in cycles, and a high-strength high-biocompatibility tough hydrogel with high order, high crystallinity and anisotropy is prepared. The preparation method is simple and easy to operate, low in cost and high in commercial value; the preparation method is combined with a 3D printing technology to prepare a customized high-strength high-biocompatibility repair bracket. The tough hydrogel prepared by the above method is applied to tissue engineering and regenerative medicine research, can realize mechanical compensation conduction force, and due to obvious toughness and biocompatibility, in-vivo self-degradability and similarity with natural tissue components, is directly sutured to a broken tendon of an adult rabbit, and tendon tissue repair is completed within 8 weeks. In addition, liquid metal can be injected into the tough hydrogel to realize detection of various parts of the human body.

A wearable ultrasound apparatus for the diagnosis and treatment of pathological tendon tissue is provided including a flexible transducer array housing comprising a plurality of transducer sub-arrays further comprised of a plurality of piezoelectric transducer elements. Wherein the flexible transducer housing includes at least two or more transducer sub-arrays the placed within adjacent segments, each segment separated by a flexible hinge that allows for contouring of the array to a patient's anatomy. The bottom side of the flexible transducer housing being secured to an acoustic impedance layer and the bottom side of the acoustic impedance layer being secured to an adhesive layer. The adhesive layer holds the flexible transducer housing and the entirety of its contents onto a patient's skin for imaging. The wearable ultrasound diagnosis and therapeutic apparatus is multifunctional with improved real time accuracy in targeting of pathological tendon tissue that is modular, flexible, wearable, and user and patient friendly.

The invention discloses a knotting-free biceps brachii long head tendon anchoring system which comprises a fastener and a locking clamping piece, one end of the fastener is installed on a bone, the locking clamping piece is connected with the other end of the fastener, the locking clamping piece comprises a first part and a second part which can be relatively separated, and the first part is connected with the second part. One end of the first part and one end of the second part are arranged in a lockable manner; an accommodating channel is formed between the first part and the second part, and when one end of the first part and one end of the second part are locked, the tendon tissue is positioned in the accommodating channel. Through the application of the anchoring system, the anchoring system suitable for knotting-free fixation between the bone and the tendon tissue of a patient is provided, the anchoring system is particularly suitable for relative fixation of the near end of a diseased long-head tendon and the intertuberous sulcus or the peripheral bone surface of the humerus, the structure is simple, operation is convenient, the operation difficulty is reduced, the operation time is shortened, and the operation efficiency is improved. Secondary injury possibly brought to a patient by a traditional suture is avoided, and postoperative rehabilitation of the patient is facilitated.

This invention provides a pharmaceutical composition comprising: i) isolated periosteum-derived stem / progenitor cells, wherein said cells have reduced PIEZO1 levels or activity; and ii) a pharmaceutically acceptable carrier. This invention also provides a method for inducing differentiation of periosteum-derived stem / progenitor cells into tendon tissue and a method for treating tendon or ligament injuries or defects with periosteum-derived stem / progenitor cells.

The application discloses a medical collagen extraction method and belongs to the technical field of biomedical materials. The extraction steps are as follows: after removing surface foreign matters from a bovine Achilles tendon, the bovine Achilles tendon is cut into particles, washed by a NaCl solution and soaked by a Tris-HCl buffer solution containing EDTA-Na2; the pretreated bovine Achilles tendon tissue is added into a swelling treatment liquid prepared by taking lactic acid and betaine as raw materials to perform soaking, so that swelled bovine Achilles tendon tissue is obtained; the swelled bovine Achilles tendon tissue is subjected to enzymolysis by pepsin, so that a crude collagen extraction liquid is obtained; poly(N-isopropyl acrylamide) is added into the crude collagen extraction liquid to precipitate a collagen-containing precipitate; after resuspension of the precipitate, constant-volume ultrafiltration diafiltration purification is performed, so that a collagen concentrate liquid is obtained, and the medical collagen extraction is completed. The product has the characteristics of high purity, structural integrity and low endotoxin, meets medical-grade safety standards and is suitable for large-scale popularization and use.