13 results about "Tendon tissue" patented technology

The invention belongs to the field of tendon tissue scaffold preparation, and provides a tendon tissue scaffold preparation method based on a decellularization technology. Although the existing decellularization technology can realize removal of cell components and retention of a matrix structure, contradiction still exists in the aspects of considering decellularization efficiency and matrix integrity. The method comprises three steps of freeze thawing treatment, trypsin digestion ultrasonic treatment and nuclease treatment, and perfect balance between decellularization efficiency and matrix retention is realized by optimizing freeze thawing parameters, regulating ultrasonic conditions and reasonably matching enzyme types and concentrations. According to the method, the cell structure can be accurately destroyed, the release of cell contents is promoted, and meanwhile, the extracellular matrix structure and bioactive molecules are protected. Experiments show that the tendon tissue scaffold prepared by the invention is thorough in cell removal, retains an extracellular matrix structure of natural tendons to the greatest extent, is closer to natural tissues in mechanical properties and biological functions, and provides a new thought for tendon repair research and application.

The invention relates to the technical field of medical surgery, and discloses an improved parachute technology and bone marrow stimulation combined rotator cuff repair method, which comprises the following steps: establishing at least five operation admission paths: a posterior lateral admission path, an anterior lateral admission path, a far-end anterior lateral admission path, a posterior lateral front admission path and a BMS (Battery Management System) and anchor channel admission path, and bone marrow stimulation operation is carried out in the rotator cuff footprint area, specifically, a drill bit with the diameter being 2 mm is used for drilling holes with the hole depth being 5 mm and the interval being 5 mm till bone marrow seeps out, and at least one inner side anchor nail is placed at the position about 2 mm away from the outer edge of the joint cartilage of the humerus head and close to the front edge of the rotator cuff tear. According to the improved parachute technology and bone marrow stimulation combined rotator cuff repairing method, the improved Mason-Allen suture bridge technology and the bone marrow stimulation technology are organically combined, a parachute suture structure is introduced, double optimization of mechanical stability and biological healing is achieved, meanwhile, the parachute structure only needs three times of threading, the threading path is clear, the operation is simple, and the operation is convenient. The operation difficulty is obviously reduced, the operation time is shortened, and the iatrogenic injury to the tendon tissue is reduced.

Surgical mesh material, especially for use in hernia surgery, comprising biological tendon tissue and manufacturing processes therefor, and the use of a mesh graft device.

The invention relates to the technical field of biochemistry and biological materials, in particular to a method for extracting high-activity I-type and III-type collagen from bovine achilles tendon tissues. The method aims at solving the technical problems that in the extraction process, a collagen triple helix active structure is difficult to completely extract, and inactivation and heterologous collagen immunological rejection are easily caused by high temperature. According to the invention, oligopeptide is introduced to protect a triple helix structure and a functional segment of collagen, so that the immunogenicity is reduced. According to the method, the complex enzyme is added, the pretreated bovine achilles tendons are subjected to enzymolysis under the low-temperature condition, and the complete triple-helix structures of the I-type collagen and the III-type collagen are protected. According to the method disclosed by the invention, the stability of the collagen structure is regulated and controlled by utilizing the difference of denaturation temperatures of the type I collagen and the type III collagen and controlling the temperature gradient in the purification stage, so that the collagen with high stability and low immunogenicity is obtained, and the complete triple-helix structure and biological activity are kept; the method can be applied to beauty injection, skin soft tissue filling, tissue repair and the like in the field of medical beauty.

The present invention discloses a quercetin nanofiber anti-inflammatory hydrogel, its preparation method and application, including the steps: a hydrogel is obtained by physically mixing nanofibers formed by self-assembly of the natural flavonoid quercetin with polyvinyl alcohol and then performing orientation freezing, salting out, pre-stretching and swelling strategies. Among them, the introduction of quercetin nanofibers improves the mechanical strength of the hydrogel and a more oriented network structure, and also effectively alleviates the inflammatory reaction brought about after implantation in the body based on its excellent antioxidant and anti-inflammatory effects. Then, salting out, pre-stretching and swelling enable the hydrogel to achieve the mechanical properties required for tendons and good biocompatibility, so as to be more effectively applied to the regeneration of tendon tissue structure.

The application provides a high-strength high-biocompatibility tough hydrogel and a preparation method and application thereof. The preparation method is a method of repeatedly stretching in a sulfate solution and soaking in a PBS buffer solution in cycles, and a high-strength high-biocompatibility tough hydrogel with high order, high crystallinity and anisotropy is prepared. The preparation method is simple and easy to operate, low in cost and high in commercial value; the preparation method is combined with a 3D printing technology to prepare a customized high-strength high-biocompatibility repair bracket. The tough hydrogel prepared by the above method is applied to tissue engineering and regenerative medicine research, can realize mechanical compensation conduction force, and due to obvious toughness and biocompatibility, in-vivo self-degradability and similarity with natural tissue components, is directly sutured to a broken tendon of an adult rabbit, and tendon tissue repair is completed within 8 weeks. In addition, liquid metal can be injected into the tough hydrogel to realize detection of various parts of the human body.

A wearable ultrasound apparatus for the diagnosis and treatment of pathological tendon tissue is provided including a flexible transducer array housing comprising a plurality of transducer sub-arrays further comprised of a plurality of piezoelectric transducer elements. Wherein the flexible transducer housing includes at least two or more transducer sub-arrays the placed within adjacent segments, each segment separated by a flexible hinge that allows for contouring of the array to a patient's anatomy. The bottom side of the flexible transducer housing being secured to an acoustic impedance layer and the bottom side of the acoustic impedance layer being secured to an adhesive layer. The adhesive layer holds the flexible transducer housing and the entirety of its contents onto a patient's skin for imaging. The wearable ultrasound diagnosis and therapeutic apparatus is multifunctional with improved real time accuracy in targeting of pathological tendon tissue that is modular, flexible, wearable, and user and patient friendly.

The invention discloses a knotting-free biceps brachii long head tendon anchoring system which comprises a fastener and a locking clamping piece, one end of the fastener is installed on a bone, the locking clamping piece is connected with the other end of the fastener, the locking clamping piece comprises a first part and a second part which can be relatively separated, and the first part is connected with the second part. One end of the first part and one end of the second part are arranged in a lockable manner; an accommodating channel is formed between the first part and the second part, and when one end of the first part and one end of the second part are locked, the tendon tissue is positioned in the accommodating channel. Through the application of the anchoring system, the anchoring system suitable for knotting-free fixation between the bone and the tendon tissue of a patient is provided, the anchoring system is particularly suitable for relative fixation of the near end of a diseased long-head tendon and the intertuberous sulcus or the peripheral bone surface of the humerus, the structure is simple, operation is convenient, the operation difficulty is reduced, the operation time is shortened, and the operation efficiency is improved. Secondary injury possibly brought to a patient by a traditional suture is avoided, and postoperative rehabilitation of the patient is facilitated.

The present invention discloses a two-component bone-inducing collagen membrane and a preparation method thereof. The collagen membrane is prepared by blending and cross-linking collagen extracted from the skin or tendon tissue of pigs or cattle with bone morphogenetic protein-2, and then injecting the mixture into a prefabricated mold and freeze-drying. The specific preparation method comprises cutting the animal tissue into thin slices, performing enzymatic hydrolysis, filtering, and precipitation to obtain collagen, which is then redissolved, and EDC and NHS cross-linking agents are added in batches. The collagen is then blended with bone morphogenetic protein-2, and finally injection-molded, freeze-dried, and irradiated for sterilization to obtain a bone morphogenetic protein + collagen two-component structure membrane. The collagen membrane not only has excellent spatial maintenance ability, but also can control the amount of BMP added and the degradation time of the product, and has good medical and practical value.

The application provides a biomechanically adaptive Achilles tendon support system capable of monitoring the repair state, which comprises a negative Poisson's ratio structure body in a sheet shape with microneedles, which is used for fixedly adhering to the Achilles tendon and covering the gap of the Achilles tendon end. When the Achilles tendon is stretched, the cross-sectional area of the Achilles tendon is increased, and through the design of the structural parameters, the Achilles tendon support system of the application is elongated along the length direction of the Achilles tendon and is circumferentially deformed and expanded with the stretching of the Achilles tendon, so as to be matched with the deformation of the cross-sectional area of the Achilles tendon. The negative Poisson's ratio structure body is a patch structure, which has good adhesion to the Achilles tendon. The Achilles tendon support system is provided with a biosensor, the sensor can sense the deformation response of the Achilles tendon tissue, and the repair state thereof is monitored. The Achilles tendon support system can be self-degraded by introducing degradable materials, and will not remain in the body as a foreign body.

This invention provides a pharmaceutical composition comprising: i) isolated periosteum-derived stem / progenitor cells, wherein said cells have reduced PIEZO1 levels or activity; and ii) a pharmaceutically acceptable carrier. This invention also provides a method for inducing differentiation of periosteum-derived stem / progenitor cells into tendon tissue and a method for treating tendon or ligament injuries or defects with periosteum-derived stem / progenitor cells.

The invention relates to a preparation method of I-type collagen, which comprises the following steps: pretreatment of raw materials: obtaining animal tendon tissues, disinfecting and sterilizing the animal tendon tissues, and removing impurities in the animal tendon tissues; collagen extraction: crushing the pretreated tendon tissues, adding dilute acid and protease, and mixing and stirring to obtain a collagen extracting solution; primary centrifugation: centrifuging the collagen extracting solution, collecting supernate, precipitating and redissolving; standing the supernate and then collecting fibrous white precipitates; redissolving the fibrous white precipitate by using an acid solution, carrying out secondary centrifugation on the redissolved solution, collecting the supernatant, and repeating the operation until the conductivity of the obtained solution is 380-480 [mu] s / cm; and freeze-drying: spreading the solution subjected to precipitation and redissolution treatment to a certain thickness, and freeze-drying to form the collagen freeze-dried tissue. According to the method, the use of hydrochloric acid is reduced, the pH value does not need to be adjusted after enzymolysis, high-concentration collagen can be separated out by adding water, and the discharge amount of salt and neutralization liquid is reduced.

The application discloses a medical collagen extraction method and belongs to the technical field of biomedical materials. The extraction steps are as follows: after removing surface foreign matters from a bovine Achilles tendon, the bovine Achilles tendon is cut into particles, washed by a NaCl solution and soaked by a Tris-HCl buffer solution containing EDTA-Na2; the pretreated bovine Achilles tendon tissue is added into a swelling treatment liquid prepared by taking lactic acid and betaine as raw materials to perform soaking, so that swelled bovine Achilles tendon tissue is obtained; the swelled bovine Achilles tendon tissue is subjected to enzymolysis by pepsin, so that a crude collagen extraction liquid is obtained; poly(N-isopropyl acrylamide) is added into the crude collagen extraction liquid to precipitate a collagen-containing precipitate; after resuspension of the precipitate, constant-volume ultrafiltration diafiltration purification is performed, so that a collagen concentrate liquid is obtained, and the medical collagen extraction is completed. The product has the characteristics of high purity, structural integrity and low endotoxin, meets medical-grade safety standards and is suitable for large-scale popularization and use.